3p3f - Revision history

OCA at 11:20, 21 February 2024

OCA — Wed, 21 Feb 2024 11:20:26 GMT

←Older revision		Revision as of 11:20, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='3p3f' size='340' side='right'caption='[[3p3f]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3p3f' size='340' side='right'caption='[[3p3f]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3p3f]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"streptomyces_cattleya"_kahan_et_al._1979 "streptomyces~~ cattleya~~" kahan et al. 1979~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P3F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3P3F FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3p3f]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_cattleya Streptomyces cattleya]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P3F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3P3F FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[3p2q\|3p2q]], [[3p2r\|3p2r]], [[3p2s\|3p2s]], [[3p3i\|3p3i]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">flK ([https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=29303 "Streptomyces cattleya" Kahan et al. 1979])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3p3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p3f OCA], [https://pdbe.org/3p3f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3p3f RCSB], [https://www.ebi.ac.uk/pdbsum/3p3f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3p3f ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3p3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p3f OCA], [https://pdbe.org/3p3f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3p3f RCSB], [https://www.ebi.ac.uk/pdbsum/3p3f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3p3f ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/FLK_STRCT FLK_STRCT]] Hydrolyzes fluoroacetyl-CoA before it can react with citrate synthase, and thus confers fluoroacetate resistance. Can not use acetyl-CoA as substrate.<ref>PMID:16720268</ref> <ref>PMID:20836570</ref>	+	[https://www.uniprot.org/uniprot/FLK_STRCT FLK_STRCT] Hydrolyzes fluoroacetyl-CoA before it can react with citrate synthase, and thus confers fluoroacetate resistance. Can not use acetyl-CoA as substrate.<ref>PMID:16720268</ref> <ref>PMID:20836570</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	We have initiated a broad-based program aimed at understanding the molecular basis of fluorine specificity in enzymatic systems, and in this context, we report crystallographic and biochemical studies on a fluoroacetyl-coenzyme A (CoA) specific thioesterase (FlK) from Streptomyces cattleya. Our data establish that FlK is competent to protect its host from fluoroacetate toxicity in vivo and demonstrate a 10(6)-fold discrimination between fluoroacetyl-CoA (k(cat)/K(M) = 5 x 10(7) M(-1) s(-1)) and acetyl-CoA (k(cat)/K(M) = 30 M(-1) s(-1)) based on a single fluorine substitution that originates from differences in both substrate reactivity and binding. We show that Thr 42, Glu 50, and His 76 are key catalytic residues and identify several factors that influence substrate selectivity. We propose that FlK minimizes interaction with the thioester carbonyl, leading to selection against acetyl-CoA binding that can be recovered in part by new C horizontal lineO interactions in the T42S and T42C mutants. We hypothesize that the loss of these interactions is compensated by the entropic driving force for fluorinated substrate binding in a hydrophobic binding pocket created by a lid structure, containing Val 23, Leu 26, Phe 33, and Phe 36, that is not found in other structurally characterized members of this superfamily. We further suggest that water plays a critical role in fluorine specificity based on biochemical and structural studies focused on the unique Phe 36 "gate" residue, which functions to exclude water from the active site. Taken together, the findings from these studies offer molecular insights into organofluorine recognition and design of fluorine-specific enzymes.	+
-		+
-	Structural and Biochemical Studies of a Fluoroacetyl-CoA-Specific Thioesterase Reveal a Molecular Basis for Fluorine Selectivity.,Weeks AM, Coyle SM, Jinek M, Doudna JA, Chang MC Biochemistry. 2010 Oct 11. PMID:20836570<ref>PMID:20836570</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3p3f" style="background-color:#fffaf0;"></div~~>	+

	==See Also==		==See Also==
Line 26:		Line 16:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Streptomyces cattleya kahan et al. 1979]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Chang, M C.Y~~]]	+	[[Category: Streptomyces cattleya]]
-	[[Category: ~~Coyle, S M~~]]	+	[[Category: Chang MCY]]
-	[[Category: ~~Doudna, J A~~]]	+	[[Category: Coyle SM]]
-	[[Category: ~~Jinek, M~~]]	+	[[Category: Doudna JA]]
-	[[Category: ~~Weeks, A~~ M]]	+	[[Category: Jinek M]]
-	[[Category: ~~Hot dog-fold]]~~	+	[[Category: Weeks AM]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Thioesterase~~]]	+

OCA at 11:17, 18 May 2022

OCA — Wed, 18 May 2022 11:17:10 GMT

←Older revision		Revision as of 11:17, 18 May 2022
Line 1:		Line 1:

	==Crystal structure of the F36A mutant of the fluoroacetyl-CoA-specific thioesterase FlK==		==Crystal structure of the F36A mutant of the fluoroacetyl-CoA-specific thioesterase FlK==
-	<StructureSection load='3p3f' size='340' side='right' caption='[[3p3f]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='3p3f' size='340' side='right'caption='[[3p3f]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3p3f]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"streptomyces_cattleya"_kahan_et_al._1979 "streptomyces cattleya" kahan et al. 1979]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P3F OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3P3F FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3p3f]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/"streptomyces_cattleya"_kahan_et_al._1979 "streptomyces cattleya" kahan et al. 1979]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P3F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3P3F FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3p2q\|3p2q]], [[3p2r\|3p2r]], [[3p2s\|3p2s]], [[3p3i\|3p3i]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3p2q\|3p2q]], [[3p2r\|3p2r]], [[3p2s\|3p2s]], [[3p3i\|3p3i]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">flK ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29303 "Streptomyces cattleya" Kahan et al. 1979])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">flK ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29303 "Streptomyces cattleya" Kahan et al. 1979])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3p3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p3f OCA], [~~http~~://pdbe.org/3p3f PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3p3f RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3p3f PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3p3f ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3p3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p3f OCA], [https://pdbe.org/3p3f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3p3f RCSB], [https://www.ebi.ac.uk/pdbsum/3p3f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3p3f ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/FLK_STRCT FLK_STRCT]] Hydrolyzes fluoroacetyl-CoA before it can react with citrate synthase, and thus confers fluoroacetate resistance. Can not use acetyl-CoA as substrate.<ref>PMID:16720268</ref> <ref>PMID:20836570</ref>	+	[[https://www.uniprot.org/uniprot/FLK_STRCT FLK_STRCT]] Hydrolyzes fluoroacetyl-CoA before it can react with citrate synthase, and thus confers fluoroacetate resistance. Can not use acetyl-CoA as substrate.<ref>PMID:16720268</ref> <ref>PMID:20836570</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:

	==See Also==		==See Also==
-	*[[Thioesterase\|Thioesterase]]	+	*[[Thioesterase 3D structures\|Thioesterase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 27:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Streptomyces cattleya kahan et al. 1979]]		[[Category: Streptomyces cattleya kahan et al. 1979]]
		+	[[Category: Large Structures]]
	[[Category: Chang, M C.Y]]		[[Category: Chang, M C.Y]]
	[[Category: Coyle, S M]]		[[Category: Coyle, S M]]

OCA at 17:39, 4 August 2016

OCA — Thu, 04 Aug 2016 17:39:38 GMT

←Older revision		Revision as of 17:39, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of the F36A mutant of the fluoroacetyl-CoA-specific thioesterase FlK==		==Crystal structure of the F36A mutant of the fluoroacetyl-CoA-specific thioesterase FlK==
	<StructureSection load='3p3f' size='340' side='right' caption='[[3p3f]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='3p3f' size='340' side='right' caption='[[3p3f]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3p3f]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptomyces_cattleya Streptomyces~~ cattleya]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P3F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3P3F FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3p3f]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"streptomyces_cattleya"_kahan_et_al._1979 "streptomyces cattleya" kahan et al. 1979]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P3F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3P3F FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3p2q\|3p2q]], [[3p2r\|3p2r]], [[3p2s\|3p2s]], [[3p3i\|3p3i]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3p2q\|3p2q]], [[3p2r\|3p2r]], [[3p2s\|3p2s]], [[3p3i\|3p3i]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">flK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29303 Streptomyces cattleya])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">flK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29303 "Streptomyces cattleya" Kahan et al. 1979])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3p3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p3f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3p3f RCSB], [http://www.ebi.ac.uk/pdbsum/3p3f PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3p3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p3f OCA], [http://pdbe.org/3p3f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3p3f RCSB], [http://www.ebi.ac.uk/pdbsum/3p3f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3p3f ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~Q1EMV2_STRCT Q1EMV2_STRCT~~]] Hydrolyzes fluoroacetyl-CoA before it can react with citrate synthase, and thus confers fluoroacetate resistance. Can not use acetyl-CoA as substrate.<ref>PMID:16720268</ref> <ref>PMID:20836570</ref>	+	[[http://www.uniprot.org/uniprot/FLK_STRCT FLK_STRCT]] Hydrolyzes fluoroacetyl-CoA before it can react with citrate synthase, and thus confers fluoroacetate resistance. Can not use acetyl-CoA as substrate.<ref>PMID:16720268</ref> <ref>PMID:20836570</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3p3f" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 24:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Streptomyces cattleya]]	+	[[Category: Streptomyces cattleya kahan et al. 1979]]
	[[Category: Chang, M C.Y]]		[[Category: Chang, M C.Y]]
	[[Category: Coyle, S M]]		[[Category: Coyle, S M]]

OCA at 17:43, 24 December 2014

OCA — Wed, 24 Dec 2014 17:43:01 GMT

←Older revision		Revision as of 17:43, 24 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3p3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p3f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3p3f RCSB], [http://www.ebi.ac.uk/pdbsum/3p3f PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3p3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p3f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3p3f RCSB], [http://www.ebi.ac.uk/pdbsum/3p3f PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q1EMV2_STRCT Q1EMV2_STRCT]] Hydrolyzes fluoroacetyl-CoA before it can react with citrate synthase, and thus confers fluoroacetate resistance. Can not use acetyl-CoA as substrate.<ref>PMID:16720268</ref> <ref>PMID:20836570</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 11:56, 9 December 2014

OCA — Tue, 09 Dec 2014 11:56:43 GMT

←Older revision		Revision as of 11:56, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3p3f.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of the F36A mutant of the fluoroacetyl-CoA-specific thioesterase FlK==
		+	<StructureSection load='3p3f' size='340' side='right' caption='[[3p3f]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3p3f]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_cattleya Streptomyces cattleya]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P3F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3P3F FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3p2q\|3p2q]], [[3p2r\|3p2r]], [[3p2s\|3p2s]], [[3p3i\|3p3i]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">flK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29303 Streptomyces cattleya])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3p3f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p3f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3p3f RCSB], [http://www.ebi.ac.uk/pdbsum/3p3f PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We have initiated a broad-based program aimed at understanding the molecular basis of fluorine specificity in enzymatic systems, and in this context, we report crystallographic and biochemical studies on a fluoroacetyl-coenzyme A (CoA) specific thioesterase (FlK) from Streptomyces cattleya. Our data establish that FlK is competent to protect its host from fluoroacetate toxicity in vivo and demonstrate a 10(6)-fold discrimination between fluoroacetyl-CoA (k(cat)/K(M) = 5 x 10(7) M(-1) s(-1)) and acetyl-CoA (k(cat)/K(M) = 30 M(-1) s(-1)) based on a single fluorine substitution that originates from differences in both substrate reactivity and binding. We show that Thr 42, Glu 50, and His 76 are key catalytic residues and identify several factors that influence substrate selectivity. We propose that FlK minimizes interaction with the thioester carbonyl, leading to selection against acetyl-CoA binding that can be recovered in part by new C horizontal lineO interactions in the T42S and T42C mutants. We hypothesize that the loss of these interactions is compensated by the entropic driving force for fluorinated substrate binding in a hydrophobic binding pocket created by a lid structure, containing Val 23, Leu 26, Phe 33, and Phe 36, that is not found in other structurally characterized members of this superfamily. We further suggest that water plays a critical role in fluorine specificity based on biochemical and structural studies focused on the unique Phe 36 "gate" residue, which functions to exclude water from the active site. Taken together, the findings from these studies offer molecular insights into organofluorine recognition and design of fluorine-specific enzymes.

-	~~{{STRUCTURE_3p3f\| PDB=3p3f \| SCENE= }}~~	+	Structural and Biochemical Studies of a Fluoroacetyl-CoA-Specific Thioesterase Reveal a Molecular Basis for Fluorine Selectivity.,Weeks AM, Coyle SM, Jinek M, Doudna JA, Chang MC Biochemistry. 2010 Oct 11. PMID:20836570<ref>PMID:20836570</ref>

-	~~===Crystal structure~~ of the ~~F36A mutant of the fluoroacetyl-CoA-specific thioesterase FlK===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_20836570}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3p3f]] is a 6 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Streptomyces_cattleya Streptomyces cattleya]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3P3F OCA].~~	+

	==See Also==		==See Also==
	*[[Thioesterase\|Thioesterase]]		*[[Thioesterase\|Thioesterase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:020836570<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Streptomyces cattleya]]		[[Category: Streptomyces cattleya]]
-	[[Category: Chang, M C.Y.]]	+	[[Category: Chang, M C.Y]]
-	[[Category: Coyle, S M.]]	+	[[Category: Coyle, S M]]
-	[[Category: Doudna, J A.]]	+	[[Category: Doudna, J A]]
-	[[Category: Jinek, M.]]	+	[[Category: Jinek, M]]
-	[[Category: Weeks, A M.]]	+	[[Category: Weeks, A M]]
	[[Category: Hot dog-fold]]		[[Category: Hot dog-fold]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Thioesterase]]		[[Category: Thioesterase]]

OCA at 07:38, 20 October 2012

OCA — Sat, 20 Oct 2012 07:38:17 GMT

←Older revision		Revision as of 07:38, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[3p3f]] is a 6 chain structure ~~of [[Thioesterase]]~~ with sequence from [http://en.wikipedia.org/wiki/Streptomyces_cattleya Streptomyces cattleya]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P3F OCA].	+	[[3p3f]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_cattleya Streptomyces cattleya]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P3F OCA].

	==See Also==		==See Also==

OCA at 16:57, 25 July 2012

OCA — Wed, 25 Jul 2012 16:57:48 GMT

←Older revision		Revision as of 16:57, 25 July 2012
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	[[Image:3p3f.png\|left\|200px]]		[[Image:3p3f.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_3p3f\| PDB=3p3f \| SCENE= }}		{{STRUCTURE_3p3f\| PDB=3p3f \| SCENE= }}

	===Crystal structure of the F36A mutant of the fluoroacetyl-CoA-specific thioesterase FlK===		===Crystal structure of the F36A mutant of the fluoroacetyl-CoA-specific thioesterase FlK===

-
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	{{ABSTRACT_PUBMED_20836570}}		{{ABSTRACT_PUBMED_20836570}}

	==About this Structure==		==About this Structure==
-	[[3p3f]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_cattleya Streptomyces cattleya]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P3F OCA].	+	[[3p3f]] is a 6 chain structure of [[Thioesterase]] with sequence from [http://en.wikipedia.org/wiki/Streptomyces_cattleya Streptomyces cattleya]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P3F OCA].
		+
		+	==See Also==
		+	*[[Thioesterase\|Thioesterase]]

	==Reference==		==Reference==

OCA: Protected "3p3f" [edit=sysop:move=sysop]

OCA — Wed, 16 Nov 2011 11:03:29 GMT

Protected "3p3f" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:03, 16 November 2011

OCA at 11:03, 16 November 2011

OCA — Wed, 16 Nov 2011 11:03:27 GMT

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	[[Image:3p3f.png\|left\|200px]]		[[Image:3p3f.png\|left\|200px]]

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	==About this Structure==		==About this Structure==
-	~~3P3F~~ is a 6 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Streptomyces_cattleya Streptomyces cattleya]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P3F OCA].	+	[[3p3f]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_cattleya Streptomyces cattleya]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P3F OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~20836570~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:020836570</ref><references group="xtra"/>
	[[Category: Streptomyces cattleya]]		[[Category: Streptomyces cattleya]]
	[[Category: Chang, M C.Y.]]		[[Category: Chang, M C.Y.]]
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	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Thioesterase]]		[[Category: Thioesterase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 10 06:56:38 2010''

OCA at 03:45, 10 November 2010

OCA — Wed, 10 Nov 2010 03:45:43 GMT

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