3p9s - Revision history

OCA at 13:29, 1 March 2024

OCA — Fri, 01 Mar 2024 13:29:53 GMT

←Older revision		Revision as of 13:29, 1 March 2024
Line 3:		Line 3:
	<StructureSection load='3p9s' size='340' side='right'caption='[[3p9s]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3p9s' size='340' side='right'caption='[[3p9s]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3p9s]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P9S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3P9S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3p9s]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P9S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3P9S FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=HDD:CIS~~-~~HEME+D+HYDROXYCHLORIN+GAMMA-SPIROLACTONE'>HDD</scene>~~, ~~<scene name='pdbligand=HDE:CIS-HEME+D+HYDROXYCHLORIN+GAMMA-SPIROLACTONE+17R,+18S'>HDE</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~OCS~~:~~CYSTEINESULFONIC~~+~~ACID~~'>~~OCS~~</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HDD:CIS-HEME+D+HYDROXYCHLORIN+GAMMA-SPIROLACTONE'>HDD</scene>, <scene name='pdbligand=HDE:CIS-HEME+D+HYDROXYCHLORIN+GAMMA-SPIROLACTONE+17R,+18S'>HDE</scene>, <scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b></td><td class="sblockDat"><div style='overflow: auto; max~~-~~height: 3em;'>[[3p9p\|3p9p]]~~, ~~[[3p9q\|3p9q]], [[3p9r\|3p9r]]</div></td></tr>~~	+
-	~~<tr id='gene~~'>~~<td class="sblockLbl"><b>[[Gene\|Gene:]]~~</b>~~</td><td class="sblockDat">b1732~~, ~~JW1721, katE ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])~~<~~/td></tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Catalase Catalase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3p9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p9s OCA], [https://pdbe.org/3p9s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3p9s RCSB], [https://www.ebi.ac.uk/pdbsum/3p9s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3p9s ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3p9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p9s OCA], [https://pdbe.org/3p9s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3p9s RCSB], [https://www.ebi.ac.uk/pdbsum/3p9s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3p9s ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CATE_ECOLI CATE_ECOLI]] Decomposes hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide.	+	[https://www.uniprot.org/uniprot/CATE_ECOLI CATE_ECOLI] Decomposes hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Heme-containing catalases have been extensively studied, revealing the roles of many residues, the existence of two heme orientations, flipped 180 degrees relative to one another along the propionate-vinyl axis, and the presence of both heme b and heme d. The focus of this report is a residue, situated adjacent to the vinyl groups of the heme at the entrance of the lateral channel, with an unusual main chain geometry that is conserved in all catalase structures so far determined. In Escherichia coli catalase HPII, the residue is Ile274, and replacing it with Gly, Ala, and Val, found at the same location in other catalases, results in a reduction in catalytic efficiency, a reduced intensity of the Soret absorbance band, and a mixture of heme orientations and species. The reduced turnover rates and higher H(2)O(2) concentrations required to attain equivalent reaction velocities are explained in terms of less efficient containment of substrate H(2)O(2) in the heme cavity arising from easier escape through the more open entrance to the lateral channel created by the smaller side chains of Gly and Ala. Inserting a Cys at position 274 resulted in the heme being covalently linked to the protein through a Cys-vinyl bond that is hypersensitive to X-ray irradiation being largely degraded within seconds of exposure to the X-ray beam. Two heme orientations, flipped along the propionate-vinyl axis, are found in the Ala, Val, and Cys variants.	+
-		+
-	Modulation of heme orientation and binding by a single residue in catalase HPII of Escherichia coli.,Jha V, Louis S, Chelikani P, Carpena X, Donald LJ, Fita I, Loewen PC Biochemistry. 2011 Mar 29;50(12):2101-10. Epub 2011 Mar 3. PMID:21332158<ref>PMID:21332158</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3p9s" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Catalase 3D structures\|Catalase 3D structures]]		*[[Catalase 3D structures\|Catalase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Catalase]]~~	+	[[Category: Escherichia coli K-12]]
-	~~[[Category: Ecoli~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Carpena, X]]	+	[[Category: Carpena X]]
-	[[Category: Chelikani, P]]	+	[[Category: Chelikani P]]
-	[[Category: Fita, I]]	+	[[Category: Fita I]]
-	[[Category: Jha, V]]	+	[[Category: Jha V]]
-	[[Category: Loewen~~, P C~~]]	+	[[Category: Loewen PC]]
-	[[Category: Louis, S]]	+	[[Category: Louis S]]
-	~~[[Category: Heme orientation]]~~	+
-	~~[[Category: I274a variant]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 08:30, 25 May 2022

OCA — Wed, 25 May 2022 08:30:47 GMT

←Older revision		Revision as of 08:30, 25 May 2022
Line 1:		Line 1:

	==Structure of I274A variant of E. coli KatE==		==Structure of I274A variant of E. coli KatE==
-	<StructureSection load='3p9s' size='340' side='right' caption='[[3p9s]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='3p9s' size='340' side='right'caption='[[3p9s]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3p9s]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P9S OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3P9S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3p9s]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P9S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3P9S FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HDD:CIS-HEME+D+HYDROXYCHLORIN+GAMMA-SPIROLACTONE'>HDD</scene>, <scene name='pdbligand=HDE:CIS-HEME+D+HYDROXYCHLORIN+GAMMA-SPIROLACTONE+17R,+18S'>HDE</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HDD:CIS-HEME+D+HYDROXYCHLORIN+GAMMA-SPIROLACTONE'>HDD</scene>, <scene name='pdbligand=HDE:CIS-HEME+D+HYDROXYCHLORIN+GAMMA-SPIROLACTONE+17R,+18S'>HDE</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3p9p\|3p9p]], [[3p9q\|3p9q]], [[3p9r\|3p9r]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3p9p\|3p9p]], [[3p9q\|3p9q]], [[3p9r\|3p9r]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b1732, JW1721, katE ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b1732, JW1721, katE ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Catalase Catalase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Catalase Catalase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3p9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p9s OCA], [~~http~~://pdbe.org/3p9s PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3p9s RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3p9s PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3p9s ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3p9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p9s OCA], [https://pdbe.org/3p9s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3p9s RCSB], [https://www.ebi.ac.uk/pdbsum/3p9s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3p9s ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CATE_ECOLI CATE_ECOLI]] Decomposes hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide.	+	[[https://www.uniprot.org/uniprot/CATE_ECOLI CATE_ECOLI]] Decomposes hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	</div>		</div>
	<div class="pdbe-citations 3p9s" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3p9s" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Catalase 3D structures\|Catalase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 28:		Line 31:
	[[Category: Catalase]]		[[Category: Catalase]]
	[[Category: Ecoli]]		[[Category: Ecoli]]
		+	[[Category: Large Structures]]
	[[Category: Carpena, X]]		[[Category: Carpena, X]]
	[[Category: Chelikani, P]]		[[Category: Chelikani, P]]

OCA at 08:57, 8 November 2017

OCA — Wed, 08 Nov 2017 08:57:19 GMT

←Older revision		Revision as of 08:57, 8 November 2017
Line 22:		Line 22:
	</div>		</div>
	<div class="pdbe-citations 3p9s" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3p9s" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Catalase\|Catalase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 22:49, 4 August 2016

OCA — Thu, 04 Aug 2016 22:49:30 GMT

←Older revision		Revision as of 22:49, 4 August 2016
Line 1:		Line 1:
		+
	==Structure of I274A variant of E. coli KatE==		==Structure of I274A variant of E. coli KatE==
	<StructureSection load='3p9s' size='340' side='right' caption='[[3p9s]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3p9s' size='340' side='right' caption='[[3p9s]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3p9s]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia coli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P9S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3P9S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3p9s]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P9S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3P9S FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HDD:CIS-HEME+D+HYDROXYCHLORIN+GAMMA-SPIROLACTONE'>HDD</scene>, <scene name='pdbligand=HDE:CIS-HEME+D+HYDROXYCHLORIN+GAMMA-SPIROLACTONE+17R,+18S'>HDE</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HDD:CIS-HEME+D+HYDROXYCHLORIN+GAMMA-SPIROLACTONE'>HDD</scene>, <scene name='pdbligand=HDE:CIS-HEME+D+HYDROXYCHLORIN+GAMMA-SPIROLACTONE+17R,+18S'>HDE</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3p9p\|3p9p]], [[3p9q\|3p9q]], [[3p9r\|3p9r]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3p9p\|3p9p]], [[3p9q\|3p9q]], [[3p9r\|3p9r]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b1732, JW1721, katE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~562 Escherichia coli~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b1732, JW1721, katE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Catalase Catalase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Catalase Catalase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3p9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p9s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3p9s RCSB], [http://www.ebi.ac.uk/pdbsum/3p9s PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3p9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p9s OCA], [http://pdbe.org/3p9s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3p9s RCSB], [http://www.ebi.ac.uk/pdbsum/3p9s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3p9s ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 20:		Line 21:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3p9s" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 28:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Catalase]]		[[Category: Catalase]]
-	[[Category: ~~Escherichia coli~~]]	+	[[Category: Ecoli]]
	[[Category: Carpena, X]]		[[Category: Carpena, X]]
	[[Category: Chelikani, P]]		[[Category: Chelikani, P]]

OCA at 13:45, 25 December 2014

OCA — Thu, 25 Dec 2014 13:45:16 GMT

←Older revision		Revision as of 13:45, 25 December 2014
Line 10:		Line 10:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3p9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p9s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3p9s RCSB], [http://www.ebi.ac.uk/pdbsum/3p9s PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3p9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p9s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3p9s RCSB], [http://www.ebi.ac.uk/pdbsum/3p9s PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CATE_ECOLI CATE_ECOLI]] Decomposes hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 12:36, 9 December 2014

OCA — Tue, 09 Dec 2014 12:36:45 GMT

←Older revision		Revision as of 12:36, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3p9s.~~png~~\|~~left~~\|~~200px~~]]	+	==Structure of I274A variant of E. coli KatE==
		+	<StructureSection load='3p9s' size='340' side='right' caption='[[3p9s]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3p9s]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P9S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3P9S FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HDD:CIS-HEME+D+HYDROXYCHLORIN+GAMMA-SPIROLACTONE'>HDD</scene>, <scene name='pdbligand=HDE:CIS-HEME+D+HYDROXYCHLORIN+GAMMA-SPIROLACTONE+17R,+18S'>HDE</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OCS:CYSTEINESULFONIC+ACID'>OCS</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3p9p\|3p9p]], [[3p9q\|3p9q]], [[3p9r\|3p9r]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">b1732, JW1721, katE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Catalase Catalase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.6 1.11.1.6] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3p9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p9s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3p9s RCSB], [http://www.ebi.ac.uk/pdbsum/3p9s PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Heme-containing catalases have been extensively studied, revealing the roles of many residues, the existence of two heme orientations, flipped 180 degrees relative to one another along the propionate-vinyl axis, and the presence of both heme b and heme d. The focus of this report is a residue, situated adjacent to the vinyl groups of the heme at the entrance of the lateral channel, with an unusual main chain geometry that is conserved in all catalase structures so far determined. In Escherichia coli catalase HPII, the residue is Ile274, and replacing it with Gly, Ala, and Val, found at the same location in other catalases, results in a reduction in catalytic efficiency, a reduced intensity of the Soret absorbance band, and a mixture of heme orientations and species. The reduced turnover rates and higher H(2)O(2) concentrations required to attain equivalent reaction velocities are explained in terms of less efficient containment of substrate H(2)O(2) in the heme cavity arising from easier escape through the more open entrance to the lateral channel created by the smaller side chains of Gly and Ala. Inserting a Cys at position 274 resulted in the heme being covalently linked to the protein through a Cys-vinyl bond that is hypersensitive to X-ray irradiation being largely degraded within seconds of exposure to the X-ray beam. Two heme orientations, flipped along the propionate-vinyl axis, are found in the Ala, Val, and Cys variants.

-	~~{{STRUCTURE_3p9s\| PDB=3p9s \| SCENE= }}~~	+	Modulation of heme orientation and binding by a single residue in catalase HPII of Escherichia coli.,Jha V, Louis S, Chelikani P, Carpena X, Donald LJ, Fita I, Loewen PC Biochemistry. 2011 Mar 29;50(12):2101-10. Epub 2011 Mar 3. PMID:21332158<ref>PMID:21332158</ref>

-	~~===Structure~~ of ~~I274A variant~~ of E. ~~coli KatE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_21332158}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3p9s]] is a 4 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P9S OCA].~~	+

	==See Also==		==See Also==
	*[[Catalase\|Catalase]]		*[[Catalase\|Catalase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:021332158<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Catalase]]		[[Category: Catalase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Carpena, X.]]	+	[[Category: Carpena, X]]
-	[[Category: Chelikani, P.]]	+	[[Category: Chelikani, P]]
-	[[Category: Fita, I.]]	+	[[Category: Fita, I]]
-	[[Category: Jha, V.]]	+	[[Category: Jha, V]]
-	[[Category: Loewen, P C.]]	+	[[Category: Loewen, P C]]
-	[[Category: Louis, S~~.]]~~	+	[[Category: Louis, S]]
-	~~[[Category: Catalase~~]]	+
	[[Category: Heme orientation]]		[[Category: Heme orientation]]
	[[Category: I274a variant]]		[[Category: I274a variant]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 17:24, 20 October 2012

OCA — Sat, 20 Oct 2012 17:24:36 GMT

←Older revision		Revision as of 17:24, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[3p9s]] is a 4 chain structure ~~of [[Catalase]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P9S OCA].	+	[[3p9s]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P9S OCA].

	==See Also==		==See Also==

OCA at 00:47, 27 July 2012

OCA — Fri, 27 Jul 2012 00:47:54 GMT

←Older revision		Revision as of 00:47, 27 July 2012
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	[[Image:3p9s.png\|left\|200px]]		[[Image:3p9s.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_3p9s\| PDB=3p9s \| SCENE= }}		{{STRUCTURE_3p9s\| PDB=3p9s \| SCENE= }}

	===Structure of I274A variant of E. coli KatE===		===Structure of I274A variant of E. coli KatE===

-
-	<!--
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	{{ABSTRACT_PUBMED_21332158}}		{{ABSTRACT_PUBMED_21332158}}

	==About this Structure==		==About this Structure==
-	[[3p9s]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P9S OCA].	+	[[3p9s]] is a 4 chain structure of [[Catalase]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P9S OCA].
		+
		+	==See Also==
		+	*[[Catalase\|Catalase]]

	==Reference==		==Reference==

OCA: Protected "3p9s" [edit=sysop:move=sysop]

OCA — Wed, 27 Jul 2011 04:55:52 GMT

Protected "3p9s" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:55, 27 July 2011

OCA at 04:55, 27 July 2011

OCA — Wed, 27 Jul 2011 04:55:51 GMT

←Older revision		Revision as of 04:55, 27 July 2011
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-	~~{{Seed}}~~	+	[[Image:3p9s.png\|left\|200px]]
-	[[Image:3p9s.~~jpg~~\|left\|200px]]	+

	<!--		<!--
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	===Structure of I274A variant of E. coli KatE===		===Structure of I274A variant of E. coli KatE===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21332158}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21332158 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21332158}}

	==About this Structure==		==About this Structure==
-	~~3P9S~~ is a 4 ~~chains~~ structure with ~~sequences~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P9S OCA].	+	[[3p9s]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P9S OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021332158</ref><references group="xtra"/>
	[[Category: Catalase]]		[[Category: Catalase]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
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	[[Category: I274a variant]]		[[Category: I274a variant]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 22 09:58:32 2010''