3pal - Revision history

OCA at 10:48, 13 February 2019

OCA — Wed, 13 Feb 2019 10:48:34 GMT

←Older revision		Revision as of 10:48, 13 February 2019
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pa/3pal_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pa/3pal_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 12:43, 5 August 2016

OCA — Fri, 05 Aug 2016 12:43:02 GMT

←Older revision		Revision as of 12:43, 5 August 2016
Line 1:		Line 1:
		+
	==IONIC INTERACTIONS WITH PARVALBUMINS. CRYSTAL STRUCTURE DETERMINATION OF PIKE 4.10 PARVALBUMIN IN FOUR DIFFERENT IONIC ENVIRONMENTS==		==IONIC INTERACTIONS WITH PARVALBUMINS. CRYSTAL STRUCTURE DETERMINATION OF PIKE 4.10 PARVALBUMIN IN FOUR DIFFERENT IONIC ENVIRONMENTS==
	<StructureSection load='3pal' size='340' side='right' caption='[[3pal]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='3pal' size='340' side='right' caption='[[3pal]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3pal]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Esox_lucius Esox lucius~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PAL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PAL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3pal]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Esolu Esolu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PAL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PAL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pal FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pal OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pal RCSB], [http://www.ebi.ac.uk/pdbsum/3pal PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pal FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pal OCA], [http://pdbe.org/3pal PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3pal RCSB], [http://www.ebi.ac.uk/pdbsum/3pal PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3pal ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3pal ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 27:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3pal" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Esox lucius~~]]	+	[[Category: Esolu]]
	[[Category: Declercq, J P]]		[[Category: Declercq, J P]]
	[[Category: Parello, J]]		[[Category: Parello, J]]

OCA at 18:56, 25 December 2014

OCA — Thu, 25 Dec 2014 18:56:19 GMT

←Older revision		Revision as of 18:56, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3pal]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Esox_lucius Esox lucius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PAL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PAL FirstGlance]. <br>		<table><tr><td colspan='2'>[[3pal]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Esox_lucius Esox lucius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PAL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PAL FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pal FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pal OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pal RCSB], [http://www.ebi.ac.uk/pdbsum/3pal PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pal FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pal OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pal RCSB], [http://www.ebi.ac.uk/pdbsum/3pal PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PRVB_ESOLU PRVB_ESOLU]] In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Esox lucius]]		[[Category: Esox lucius]]
-	[[Category: Declercq, J P.]]	+	[[Category: Declercq, J P]]
-	[[Category: Parello, J.]]	+	[[Category: Parello, J]]
-	[[Category: Rambaud, J.]]	+	[[Category: Rambaud, J]]
-	[[Category: Tinant, B.]]	+	[[Category: Tinant, B]]
	[[Category: Calcium binding protein]]		[[Category: Calcium binding protein]]

OCA at 13:30, 29 September 2014

OCA — Mon, 29 Sep 2014 13:30:59 GMT

←Older revision		Revision as of 13:30, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~3pal.~~png~~\|~~left~~\|200px]]	+	==IONIC INTERACTIONS WITH PARVALBUMINS. CRYSTAL STRUCTURE DETERMINATION OF PIKE 4.10 PARVALBUMIN IN FOUR DIFFERENT IONIC ENVIRONMENTS==
		+	<StructureSection load='3pal' size='340' side='right' caption='[[3pal]], [[Resolution\|resolution]] 2.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3pal]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Esox_lucius Esox lucius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PAL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PAL FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pal FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pal OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pal RCSB], [http://www.ebi.ac.uk/pdbsum/3pal PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pa/3pal_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The crystal structure of the Ca-loaded form of pike 4.10 parvalbumin (minor component from pike muscle belonging to the beta phylogenetic series), with both its primary sites CD and EF occupied by Ca2+ ions and its third site occupied by an ammonium ion, as previously determined at 1.93 A resolution, has now been refined to a resolution of 1.65 A. The crystallization of this parvalbumin in different ionic environments has allowed three novel non-isomorphous crystalline forms to be obtained: (1) a first form, crystallized in the presence of a mixture of ammonium sulphate and manganese sulphate, for which all the cation binding sites in the protein are occupied by Mn2+; (2) a second form crystallized in the presence of MgSO4 as the precipitating agent, only differs from the Ca/NH4 form by the occupation of the third site by Mg2+, whereas the primary sites remain occupied by Ca2+; (3) a third form, also crystallized in the presence of MgSO4, corresponds to a well-defined molecular species with both the primary EF site and the third site occupied by Mg2+, whereas the primary CD site remains occupied by CA2+. The corresponding molecular structures reported here have been determined at resolutions between 1.8 and 2.4 A. The comparison of the different crystal structures allows the structural modifications accompanying the substitution of the primary sites by cations differing significantly in their ionic radii (Ca2+, Mn2+, Mg2+) to be investigated in detail, and it also leads to a precise description of the third site in a typical beta parvalbumin. The substitution Ca2+ by Mg2+ within the primary site EF is characterized by a "contraction" of the co-ordination sphere, with a decrease of the mean oxygen-metal distance by a value of 0.25 A and a decrease of the co-ordination number from 7 to 6, as a consequence of the loss of a bidentate ligand (Glu101), which becomes a monodentate one. Such an adaptation of the co-ordination sphere around a cation of smaller size involves, among others, the transformation of the Glu101 side-chain from the stable gauche(+) form to the less stable gauche(-) form. The third site is clearly described as a satellite of the CD primary site, since both sites possess common protein ligands, such as Asp53 and Glu59. Furthermore, Asp61 appears as a specific ligand of the third site in the different environments investigated in this work. We finally discuss the relevance of the third site to parvalbumin phylogeny.

-	~~{{STRUCTURE_3pal\| PDB=3pal \| SCENE= }}~~	+	Ionic interactions with parvalbumins. Crystal structure determination of pike 4.10 parvalbumin in four different ionic environments.,Declercq JP, Tinant B, Parello J, Rambaud J J Mol Biol. 1991 Aug 20;220(4):1017-39. PMID:1880797<ref>PMID:1880797</ref>

-	~~===IONIC INTERACTIONS WITH PARVALBUMINS. CRYSTAL STRUCTURE DETERMINATION OF PIKE 4.10 PARVALBUMIN IN FOUR DIFFERENT IONIC ENVIRONMENTS===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_1880797}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3pal]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Esox_lucius Esox lucius]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3PAL OCA].~~	+

	==See Also==		==See Also==
	*[[Parvalbumin\|Parvalbumin]]		*[[Parvalbumin\|Parvalbumin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:001880797<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Esox lucius]]		[[Category: Esox lucius]]
	[[Category: Declercq, J P.]]		[[Category: Declercq, J P.]]

OCA at 20:14, 20 October 2012

OCA — Sat, 20 Oct 2012 20:14:49 GMT

←Older revision		Revision as of 20:14, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3pal]] is a 1 chain structure ~~of [[Parvalbumin]]~~ with sequence from [http://en.wikipedia.org/wiki/Esox_lucius Esox lucius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PAL OCA].	+	[[3pal]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Esox_lucius Esox lucius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PAL OCA].

	==See Also==		==See Also==

OCA: Protected "3pal" [edit=sysop:move=sysop]

OCA — Fri, 27 Jul 2012 09:52:07 GMT

Protected "3pal" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:52, 27 July 2012

OCA at 09:52, 27 July 2012

OCA — Fri, 27 Jul 2012 09:52:05 GMT

←Older revision		Revision as of 09:52, 27 July 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:3pal.png\|left\|200px]]		[[Image:3pal.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3pal", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3pal\| PDB=3pal \| SCENE= }}		{{STRUCTURE_3pal\| PDB=3pal \| SCENE= }}

	===IONIC INTERACTIONS WITH PARVALBUMINS. CRYSTAL STRUCTURE DETERMINATION OF PIKE 4.10 PARVALBUMIN IN FOUR DIFFERENT IONIC ENVIRONMENTS===		===IONIC INTERACTIONS WITH PARVALBUMINS. CRYSTAL STRUCTURE DETERMINATION OF PIKE 4.10 PARVALBUMIN IN FOUR DIFFERENT IONIC ENVIRONMENTS===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_1880797}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 1880797 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_1880797}}		{{ABSTRACT_PUBMED_1880797}}

	==About this Structure==		==About this Structure==
-	~~3PAL~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Esox_lucius Esox lucius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PAL OCA].	+	[[3pal]] is a 1 chain structure of [[Parvalbumin]] with sequence from [http://en.wikipedia.org/wiki/Esox_lucius Esox lucius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PAL OCA].
		+
		+	==See Also==
		+	*[[Parvalbumin\|Parvalbumin]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~1880797~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:001880797</ref><references group="xtra"/>
	[[Category: Esox lucius]]		[[Category: Esox lucius]]
	[[Category: Declercq, J P.]]		[[Category: Declercq, J P.]]
Line 30:		Line 21:
	[[Category: Tinant, B.]]		[[Category: Tinant, B.]]
	[[Category: Calcium binding protein]]		[[Category: Calcium binding protein]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 03:38:45 2009''

OCA at 01:38, 18 February 2009

OCA — Wed, 18 Feb 2009 01:38:47 GMT

←Older revision		Revision as of 01:38, 18 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	3PAL is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Esox_lucius Esox lucius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PAL OCA].	+	3PAL is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Esox_lucius Esox lucius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PAL OCA].

	==Reference==		==Reference==
-	Ionic interactions with parvalbumins. Crystal structure determination of pike 4.10 parvalbumin in four different ionic environments., Declercq JP, Tinant B, Parello J, Rambaud J, J Mol Biol. 1991 Aug 20;220(4):1017-39. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/1880797 1880797]~~	+	<ref group="xtra">PMID:1880797</ref><references group="xtra"/>
	[[Category: Esox lucius]]		[[Category: Esox lucius]]
-	[[Category: Single protein]]
	[[Category: Declercq, J P.]]		[[Category: Declercq, J P.]]
	[[Category: Parello, J.]]		[[Category: Parello, J.]]
Line 32:		Line 31:
	[[Category: Calcium binding protein]]		[[Category: Calcium binding protein]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu Jul 3 12~~:58:~~58 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 03:38:45 2009''

OCA at 09:58, 3 July 2008

OCA — Thu, 03 Jul 2008 09:58:58 GMT

←Older revision		Revision as of 09:58, 3 July 2008
Line 1:		Line 1:
-	[[Image:3pal.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:3pal.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_3pal\| PDB=3pal \| SCENE= }}		{{STRUCTURE_3pal\| PDB=3pal \| SCENE= }}

-	~~'''~~IONIC INTERACTIONS WITH PARVALBUMINS. CRYSTAL STRUCTURE DETERMINATION OF PIKE 4.10 PARVALBUMIN IN FOUR DIFFERENT IONIC ENVIRONMENTS~~'''~~	+	===IONIC INTERACTIONS WITH PARVALBUMINS. CRYSTAL STRUCTURE DETERMINATION OF PIKE 4.10 PARVALBUMIN IN FOUR DIFFERENT IONIC ENVIRONMENTS===


-	~~==Overview==~~	+	<!--
-	The crystal structure of the Ca-loaded form of pike 4.10 parvalbumin (minor component from pike muscle belonging to the beta phylogenetic series), with both its primary sites CD and EF occupied by Ca2+ ions and its third site occupied by an ammonium ion, as previously determined at 1.93 A resolution, has now been refined to a resolution of 1.65 A. The crystallization of this ~~parvalbumin in different ionic environments has allowed three novel non-isomorphous crystalline forms to be obtained: (1) a first form~~, ~~crystallized in the presence of a mixture of ammonium sulphate and manganese sulphate~~, ~~for which all~~ the ~~cation binding sites in~~ the ~~protein are occupied by Mn2+;~~ (~~2) a second form crystallized in the presence of MgSO4~~ as ~~the precipitating agent, only differs from the Ca~~/NH4 form by the occupation of the third site by Mg2+, whereas the primary sites remain occupied by Ca2+; (3) a third form, also crystallized in the presence of MgSO4, corresponds to a well-defined molecular species with both the primary EF site and the third site occupied by Mg2+, whereas the primary CD site remains occupied by CA2+. ~~The corresponding molecular structures reported here have been determined at resolutions between 1~~.8 and 2.4 A. The comparison of the different crystal structures allows the structural modifications accompanying the substitution of the primary sites by cations differing significantly in their ionic radii (Ca2+, Mn2+, Mg2+) ~~to be investigated in detail~~, ~~and it also leads to a precise description of the third site in a typical beta parvalbumin. The substitution Ca2+ by Mg2+ within the primary site EF~~ is ~~characterized by a "contraction" of~~ the ~~co-ordination sphere, with a decrease of the mean oxygen-metal distance by a value of 0.25 A and a decrease of the co-ordination~~ number ~~from 7 to 6, as a consequence of the loss of a bidentate ligand (Glu101), which becomes a monodentate one~~. ~~Such an adaptation of the co~~-~~ordination sphere around a cation of smaller size involves, among others, the transformation of the Glu101 side~~-chain from the stable gauche(+) form to the less stable gauche(-) form. The third site is clearly described as a satellite of the CD primary site, since both sites possess common protein ligands, such as Asp53 and Glu59. Furthermore, Asp61 appears as a specific ligand of the third site in the different environments investigated in this work. We finally discuss the relevance of the third site to parvalbumin phylogeny.	+	The line below this paragraph, {{ABSTRACT_PUBMED_1880797}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 1880797 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_1880797}}

	==About this Structure==		==About this Structure==
Line 27:		Line 31:
	[[Category: Tinant, B.]]		[[Category: Tinant, B.]]
	[[Category: Calcium binding protein]]		[[Category: Calcium binding protein]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May~~ ~~4 22~~:08:44 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 12:58:58 2008''

OCA at 19:08, 4 May 2008

OCA — Sun, 04 May 2008 19:08:46 GMT

←Older revision		Revision as of 19:08, 4 May 2008
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	[[Image:3pal.jpg\|left\|200px]]		[[Image:3pal.jpg\|left\|200px]]

-	~~{{Structure~~	+	<!--
-	~~\|PDB= 3pal \|SIZE=350\|CAPTION= <scene name='initialview01'>3pal</scene>~~, ~~resolution 2~~.~~4Å~~	+	The line below this paragraph, containing "STRUCTURE_3pal", creates the "Structure Box" on the page.
-	~~\|SITE=~~	+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	~~\|LIGAND= <~~scene ~~name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>~~, ~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~	+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	~~\|ACTIVITY=~~	+	or leave the SCENE parameter empty for the default display.
-	~~\|GENE=~~	+	-->
-	\|~~DOMAIN~~=	+	{{STRUCTURE_3pal\| PDB=3pal \| SCENE= }}
-	\|~~RELATEDENTRY~~=	+
-	\|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pal FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pal OCA], [http://www.ebi.ac.uk/pdbsum/3pal PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3pal RCSB]</span>	+
-	}}	+

	'''IONIC INTERACTIONS WITH PARVALBUMINS. CRYSTAL STRUCTURE DETERMINATION OF PIKE 4.10 PARVALBUMIN IN FOUR DIFFERENT IONIC ENVIRONMENTS'''		'''IONIC INTERACTIONS WITH PARVALBUMINS. CRYSTAL STRUCTURE DETERMINATION OF PIKE 4.10 PARVALBUMIN IN FOUR DIFFERENT IONIC ENVIRONMENTS'''
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	[[Category: Rambaud, J.]]		[[Category: Rambaud, J.]]
	[[Category: Tinant, B.]]		[[Category: Tinant, B.]]
-	[[Category: ~~calcium~~ binding protein]]	+	[[Category: Calcium binding protein]]
-		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:08:44 2008''
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Mar 31 05~~:34:59 2008''	+