3pcy - Revision history

OCA at 10:40, 21 February 2024

OCA — Wed, 21 Feb 2024 10:40:06 GMT

←Older revision		Revision as of 10:40, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='3pcy' size='340' side='right'caption='[[3pcy]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3pcy' size='340' side='right'caption='[[3pcy]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3pcy]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lombardy_poplar Lombardy poplar~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PCY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3PCY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3pcy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Populus_nigra Populus nigra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PCY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PCY FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3pcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pcy OCA], [~~http~~://pdbe.org/3pcy PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3pcy RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3pcy PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3pcy ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pcy OCA], [https://pdbe.org/3pcy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pcy RCSB], [https://www.ebi.ac.uk/pdbsum/3pcy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pcy ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/PLAS1_POPNI PLAS1_POPNI]] Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I.	+	[https://www.uniprot.org/uniprot/PLAS1_POPNI PLAS1_POPNI] Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3pcy ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3pcy ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The crystal structure of Hg(II)-plastocyanin has been determined and refined at a resolution of 1.9 A. The crystals were prepared by soaking crystals of Cu(II)-plastocyanin from poplar leaves (Populus nigra var. italica) in a solution of a mercuric salt. Replacement of the Cu(II) atom in plastocyanin by Hg(II) causes only minor changes in the geometry of the metal site, and there are few significant changes elsewhere in the molecule. It is concluded that, as in the case of the native protein, the geometry of the metal site is determined by the polypeptide. The weak metal-S(methionine) bond found in Cu(II)-plastocyanin remains weak in Hg(II)-plastocyanin. The "flip" of a proline side chain close to the metal site from a C gamma-exo conformation in Cu(II)-plastocyanin to a C gamma-endo conformation in Hg(II)-plastocyanin suggests that this region of the molecule is particularly flexible. Crystallographic evidence for the close similarity of the Hg(II)- and Cu(II)-plastocyanin structures was originally obtained from electron density difference maps at 2.5-A resolution. The refinement of the structure was begun with a set of atomic coordinates taken from the structure of Cu(II)-plastocyanin. A Hg(II) atom was substituted for the Cu(II) atom, and the side chains of 6 residues in the vicinity of the metal site were omitted. Three series of stereochemically restrained least-squares refinement calculations were interspersed with two stages of model adjustment followed by phase extension. Fifty-nine water molecules were located. The final structure has a crystallographic residual R = 0.16.

-	The crystal structure of mercury-substituted poplar plastocyanin at 1.9-A resolution.,Church WB, Guss JM, Potter JJ, Freeman HC J Biol Chem. 1986 Jan 5;261(1):234-7. PMID:3941073<ref>PMID:3941073</ref>	+	==See Also==
-		+	*[[Plastocyanin 3D structures\|Plastocyanin 3D structures]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3pcy" style="background-color:#fffaf0;"></div>~~	+
-	== ~~References~~ ==	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lombardy poplar~~]]	+	[[Category: Populus nigra]]
-	[[Category: Church~~, W B~~]]	+	[[Category: Church WB]]
-	[[Category: Freeman~~, H C~~]]	+	[[Category: Freeman HC]]
-	[[Category: Guss~~, J M~~]]	+	[[Category: Guss JM]]
-	[[Category: Potter~~, J J~~]]	+	[[Category: Potter JJ]]

OCA at 13:49, 25 December 2019

OCA — Wed, 25 Dec 2019 13:49:29 GMT

←Older revision		Revision as of 13:49, 25 December 2019
Line 1:		Line 1:

	==THE CRYSTAL STRUCTURE OF MERCURY-SUBSTITUTED POPLAR PLASTOCYANIN AT 1.9-ANGSTROMS RESOLUTION==		==THE CRYSTAL STRUCTURE OF MERCURY-SUBSTITUTED POPLAR PLASTOCYANIN AT 1.9-ANGSTROMS RESOLUTION==
-	<StructureSection load='3pcy' size='340' side='right' caption='[[3pcy]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='3pcy' size='340' side='right'caption='[[3pcy]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3pcy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lombardy_poplar Lombardy poplar]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PCY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PCY FirstGlance]. <br>		<table><tr><td colspan='2'>[[3pcy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lombardy_poplar Lombardy poplar]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PCY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PCY FirstGlance]. <br>
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pc/3pcy_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pc/3pcy_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 32:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Lombardy poplar]]		[[Category: Lombardy poplar]]
	[[Category: Church, W B]]		[[Category: Church, W B]]

OCA at 00:22, 5 August 2016

OCA — Fri, 05 Aug 2016 00:22:26 GMT

←Older revision		Revision as of 00:22, 5 August 2016
Line 1:		Line 1:
		+
	==THE CRYSTAL STRUCTURE OF MERCURY-SUBSTITUTED POPLAR PLASTOCYANIN AT 1.9-ANGSTROMS RESOLUTION==		==THE CRYSTAL STRUCTURE OF MERCURY-SUBSTITUTED POPLAR PLASTOCYANIN AT 1.9-ANGSTROMS RESOLUTION==
	<StructureSection load='3pcy' size='340' side='right' caption='[[3pcy]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='3pcy' size='340' side='right' caption='[[3pcy]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3pcy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Populus_nigra Populus nigra~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PCY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PCY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3pcy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lombardy_poplar Lombardy poplar]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PCY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PCY FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pcy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pcy RCSB], [http://www.ebi.ac.uk/pdbsum/3pcy PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pcy OCA], [http://pdbe.org/3pcy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3pcy RCSB], [http://www.ebi.ac.uk/pdbsum/3pcy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3pcy ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3pcy ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 26:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3pcy" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Populus nigra~~]]	+	[[Category: Lombardy poplar]]
	[[Category: Church, W B]]		[[Category: Church, W B]]
	[[Category: Freeman, H C]]		[[Category: Freeman, H C]]
	[[Category: Guss, J M]]		[[Category: Guss, J M]]
	[[Category: Potter, J J]]		[[Category: Potter, J J]]

OCA at 14:20, 25 December 2014

OCA — Thu, 25 Dec 2014 14:20:27 GMT

←Older revision		Revision as of 14:20, 25 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pcy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pcy RCSB], [http://www.ebi.ac.uk/pdbsum/3pcy PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pcy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pcy RCSB], [http://www.ebi.ac.uk/pdbsum/3pcy PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PLAS1_POPNI PLAS1_POPNI]] Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 06:52, 19 December 2014

OCA — Fri, 19 Dec 2014 06:52:50 GMT

←Older revision		Revision as of 06:52, 19 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3pcy\| PDB=3pcy \| SCENE= }}~~	+	==THE CRYSTAL STRUCTURE OF MERCURY-SUBSTITUTED POPLAR PLASTOCYANIN AT 1.9-ANGSTROMS RESOLUTION==
-	===THE CRYSTAL STRUCTURE OF MERCURY-SUBSTITUTED POPLAR PLASTOCYANIN AT 1.9-ANGSTROMS RESOLUTION===	+	<StructureSection load='3pcy' size='340' side='right' caption='[[3pcy]], [[Resolution\|resolution]] 1.90Å' scene=''>
-	~~{{ABSTRACT_PUBMED_3941073}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3pcy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Populus_nigra Populus nigra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PCY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PCY FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pcy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pcy RCSB], [http://www.ebi.ac.uk/pdbsum/3pcy PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pc/3pcy_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The crystal structure of Hg(II)-plastocyanin has been determined and refined at a resolution of 1.9 A. The crystals were prepared by soaking crystals of Cu(II)-plastocyanin from poplar leaves (Populus nigra var. italica) in a solution of a mercuric salt. Replacement of the Cu(II) atom in plastocyanin by Hg(II) causes only minor changes in the geometry of the metal site, and there are few significant changes elsewhere in the molecule. It is concluded that, as in the case of the native protein, the geometry of the metal site is determined by the polypeptide. The weak metal-S(methionine) bond found in Cu(II)-plastocyanin remains weak in Hg(II)-plastocyanin. The "flip" of a proline side chain close to the metal site from a C gamma-exo conformation in Cu(II)-plastocyanin to a C gamma-endo conformation in Hg(II)-plastocyanin suggests that this region of the molecule is particularly flexible. Crystallographic evidence for the close similarity of the Hg(II)- and Cu(II)-plastocyanin structures was originally obtained from electron density difference maps at 2.5-A resolution. The refinement of the structure was begun with a set of atomic coordinates taken from the structure of Cu(II)-plastocyanin. A Hg(II) atom was substituted for the Cu(II) atom, and the side chains of 6 residues in the vicinity of the metal site were omitted. Three series of stereochemically restrained least-squares refinement calculations were interspersed with two stages of model adjustment followed by phase extension. Fifty-nine water molecules were located. The final structure has a crystallographic residual R = 0.16.

-	~~==Function==~~	+	The crystal structure of mercury-substituted poplar plastocyanin at 1.9-A resolution.,Church WB, Guss JM, Potter JJ, Freeman HC J Biol Chem. 1986 Jan 5;261(1):234-7. PMID:3941073<ref>PMID:3941073</ref>
-	~~[[http://www~~.~~uniprot~~.~~org/uniprot/PLAS1_POPNI PLAS1_POPNI]] Participates in electron transfer between P700 and the cytochrome b6~~-~~f complex in photosystem I~~.	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3pcy]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Populus_nigra Populus nigra]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3PCY OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:003941073</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Populus nigra]]		[[Category: Populus nigra]]
-	[[Category: Church, W B.]]	+	[[Category: Church, W B]]
-	[[Category: Freeman, H C.]]	+	[[Category: Freeman, H C]]
-	[[Category: Guss, J M.]]	+	[[Category: Guss, J M]]
-	[[Category: Potter, J J.]]	+	[[Category: Potter, J J]]

OCA: Protected "3pcy" [edit=sysop:move=sysop]

OCA — Thu, 02 May 2013 08:33:46 GMT

Protected "3pcy" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:33, 2 May 2013

OCA at 08:33, 2 May 2013

OCA — Thu, 02 May 2013 08:33:43 GMT

←Older revision		Revision as of 08:33, 2 May 2013
Line 1:		Line 1:
-	{{Seed}}
-	[[Image:3pcy.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3pcy", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3pcy\| PDB=3pcy \| SCENE= }}		{{STRUCTURE_3pcy\| PDB=3pcy \| SCENE= }}
-
	===THE CRYSTAL STRUCTURE OF MERCURY-SUBSTITUTED POPLAR PLASTOCYANIN AT 1.9-ANGSTROMS RESOLUTION===		===THE CRYSTAL STRUCTURE OF MERCURY-SUBSTITUTED POPLAR PLASTOCYANIN AT 1.9-ANGSTROMS RESOLUTION===
		+	{{ABSTRACT_PUBMED_3941073}}

-		+	==Function==
-	~~<!--~~	+	[[http://www.uniprot.org/uniprot/PLAS1_POPNI PLAS1_POPNI]] Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I.
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_3941073}}, adds the Publication Abstract to the page~~	+
-	~~(as it appears on PubMed at~~ http://www.~~pubmed~~.~~gov), where 3941073 is~~ the ~~PubMed ID number~~.	+
-	~~-->~~	+
-	~~{{ABSTRACT_PUBMED_3941073}}~~	+

	==About this Structure==		==About this Structure==
-	~~3PCY~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Populus_nigra Populus nigra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PCY OCA].	+	[[3pcy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Populus_nigra Populus nigra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PCY OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~3941073~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:003941073</ref><references group="xtra"/><references/>
	[[Category: Populus nigra]]		[[Category: Populus nigra]]
	[[Category: Church, W B.]]		[[Category: Church, W B.]]
Line 29:		Line 16:
	[[Category: Guss, J M.]]		[[Category: Guss, J M.]]
	[[Category: Potter, J J.]]		[[Category: Potter, J J.]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 18:32:44 2009''

OCA at 16:32, 17 February 2009

OCA — Tue, 17 Feb 2009 16:32:45 GMT

←Older revision		Revision as of 16:32, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	3PCY is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Populus_nigra Populus nigra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PCY OCA].	+	3PCY is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Populus_nigra Populus nigra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PCY OCA].

	==Reference==		==Reference==
-	~~The crystal structure of mercury-substituted poplar plastocyanin at 1.9-A resolution., Church WB, Guss JM, Potter JJ, Freeman HC, J Biol Chem. 1986 Jan 5;261(1):234-7.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/3941073 3941073]~~	+	<ref group="xtra">PMID:3941073</ref><references group="xtra"/>
	[[Category: Populus nigra]]		[[Category: Populus nigra]]
-	[[Category: Single protein]]
	[[Category: Church, W B.]]		[[Category: Church, W B.]]
	[[Category: Freeman, H C.]]		[[Category: Freeman, H C.]]
Line 31:		Line 30:
	[[Category: Potter, J J.]]		[[Category: Potter, J J.]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu Jul 3 13~~:00:~~57 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 18:32:44 2009''

OCA at 10:00, 3 July 2008

OCA — Thu, 03 Jul 2008 10:00:58 GMT

←Older revision		Revision as of 10:00, 3 July 2008
Line 1:		Line 1:
-	[[Image:3pcy.~~jpg~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:3pcy.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_3pcy\| PDB=3pcy \| SCENE= }}		{{STRUCTURE_3pcy\| PDB=3pcy \| SCENE= }}

-	~~'''~~THE CRYSTAL STRUCTURE OF MERCURY-SUBSTITUTED POPLAR PLASTOCYANIN AT 1.9-ANGSTROMS RESOLUTION~~'''~~	+	===THE CRYSTAL STRUCTURE OF MERCURY-SUBSTITUTED POPLAR PLASTOCYANIN AT 1.9-ANGSTROMS RESOLUTION===


-	~~==Overview==~~	+	<!--
-	The crystal structure of Hg(II)-plastocyanin has been determined and refined at a resolution of 1.9 A. The crystals were prepared by soaking crystals of Cu(II)-plastocyanin from poplar leaves (Populus nigra var. italica) in a solution of a mercuric salt. Replacement of the Cu(II) atom in plastocyanin by Hg(II) causes only minor changes in the geometry of the metal site, ~~and there are few significant changes elsewhere in the molecule. It is concluded that~~, ~~as in~~ the case of the native protein, the geometry of the metal site is determined by the polypeptide. The weak metal-S(methionine) bond found in Cu(II)-plastocyanin remains weak in Hg(II)-plastocyanin. The "flip" of a proline side chain close to the ~~metal site from a C gamma-exo conformation in Cu~~(II)-plastocyanin to a C gamma-endo conformation in Hg(II)-plastocyanin suggests that this region of the molecule is particularly flexible. Crystallographic evidence for the close similarity of the Hg(II)- and Cu(II)-plastocyanin structures was originally obtained from electron density difference maps at 2.~~5-A resolution~~. ~~The refinement of the structure was begun with a set of atomic coordinates taken from the structure of Cu(II~~)~~-plastocyanin. A Hg(II) atom was substituted for the Cu(II) atom~~, ~~and~~ the ~~side chains of 6 residues in the vicinity of the metal site were omitted~~. ~~Three series of stereochemically restrained least~~-~~squares refinement calculations were interspersed with two stages of model adjustment followed by phase extension. Fifty~~-~~nine water molecules were located. The final structure has a crystallographic residual R = 0.16.~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_3941073}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 3941073 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_3941073}}

	==About this Structure==		==About this Structure==
Line 26:		Line 30:
	[[Category: Guss, J M.]]		[[Category: Guss, J M.]]
	[[Category: Potter, J J.]]		[[Category: Potter, J J.]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May~~ ~~4 22~~:09:56 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 13:00:57 2008''

OCA at 19:09, 4 May 2008

OCA — Sun, 04 May 2008 19:09:58 GMT

←Older revision		Revision as of 19:09, 4 May 2008
Line 1:		Line 1:
	[[Image:3pcy.jpg\|left\|200px]]		[[Image:3pcy.jpg\|left\|200px]]

-	~~{{Structure~~	+	<!--
-	~~\|PDB= 3pcy \|SIZE=350\|CAPTION= <scene name='initialview01'>3pcy</scene>~~, ~~resolution 1~~.~~9Å~~	+	The line below this paragraph, containing "STRUCTURE_3pcy", creates the "Structure Box" on the page.
-	~~\|SITE=~~	+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	~~\|LIGAND= <~~scene ~~name='pdbligand=HG:MERCURY+(II~~)~~+ION'>HG</scene>~~	+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	~~\|ACTIVITY=~~	+	or leave the SCENE parameter empty for the default display.
-	~~\|GENE=~~	+	-->
-	\|~~DOMAIN~~=	+	{{STRUCTURE_3pcy\| PDB=3pcy \| SCENE= }}
-	\|~~RELATEDENTRY~~=	+
-	\|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pcy OCA], [http://www.ebi.ac.uk/pdbsum/3pcy PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3pcy RCSB]</span>	+
-	}}	+

	'''THE CRYSTAL STRUCTURE OF MERCURY-SUBSTITUTED POPLAR PLASTOCYANIN AT 1.9-ANGSTROMS RESOLUTION'''		'''THE CRYSTAL STRUCTURE OF MERCURY-SUBSTITUTED POPLAR PLASTOCYANIN AT 1.9-ANGSTROMS RESOLUTION'''
Line 29:		Line 26:
	[[Category: Guss, J M.]]		[[Category: Guss, J M.]]
	[[Category: Potter, J J.]]		[[Category: Potter, J J.]]
-	~~[[Category: electron transport protein(cuproprotein)]]~~	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:09:56 2008''
-		+
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Mar 31 05~~:35:19 2008''	+