3pf4 - Revision history

OCA at 09:53, 6 September 2023

OCA — Wed, 06 Sep 2023 09:53:08 GMT

←Older revision		Revision as of 09:53, 6 September 2023
Line 3:		Line 3:
	<StructureSection load='3pf4' size='340' side='right'caption='[[3pf4]], [[Resolution\|resolution]] 1.38Å' scene=''>		<StructureSection load='3pf4' size='340' side='right'caption='[[3pf4]], [[Resolution\|resolution]] 1.38Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3pf4]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"vibrio_subtilis"_ehrenberg_1835 "vibrio~~ subtilis~~" ehrenberg 1835~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PF4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PF4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3pf4]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PF4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PF4 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~, ~~<scene name='pdbligand=NA:SODIUM+ION'>NA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.38Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1csp\|1csp]], [[2hax\|2hax]], [[2es2\|2es2]], [[2f52\|2f52]], [[1c9o\|1c9o]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">BSU09100, cspA, cspB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pf4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pf4 OCA], [https://pdbe.org/3pf4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pf4 RCSB], [https://www.ebi.ac.uk/pdbsum/3pf4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pf4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pf4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pf4 OCA], [https://pdbe.org/3pf4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pf4 RCSB], [https://www.ebi.ac.uk/pdbsum/3pf4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pf4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CSPB_BACSU CSPB_BACSU]] Binds to the pentamer sequences ATTGG and CCAAT with highest affinity in single-stranded DNA, and also to other sequences. Has greater affinity for ATTGG than CCAAT. Can act as transcriptional activator of cold shock genes by recognizing putative ATTGG-box elements present in promoter regions of genes induced under cold shock conditions.	+	[https://www.uniprot.org/uniprot/CSPB_BACSU CSPB_BACSU] Binds to the pentamer sequences ATTGG and CCAAT with highest affinity in single-stranded DNA, and also to other sequences. Has greater affinity for ATTGG than CCAAT. Can act as transcriptional activator of cold shock genes by recognizing putative ATTGG-box elements present in promoter regions of genes induced under cold shock conditions.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Vibrio~~ subtilis ~~ehrenberg 1835~~]]	+	[[Category: Bacillus subtilis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Heinemann, U]]	+	[[Category: Heinemann U]]
-	[[Category: Max~~, K E.A~~]]	+	[[Category: Max KEA]]
-	[[Category: Sachs, R]]	+	[[Category: Sachs R]]
-	~~[[Category: Beta barrel]]~~	+
-	~~[[Category: Cold shock domain]]~~	+
-	~~[[Category: Cold shock response]]~~	+
-	~~[[Category: Cytosol]]~~	+
-	~~[[Category: Gene regulation-rna complex]]~~	+
-	~~[[Category: Ob fold]]~~	+
-	~~[[Category: Protein-rna complex]]~~	+
-	~~[[Category: Rna/dna binding]]~~	+
-	~~[[Category: Single-stranded rna and dna]]~~	+
-	~~[[Category: Transcription regulation]]~~	+
-	~~[[Category: Translation regulation~~]]	+

OCA at 08:36, 25 May 2022

OCA — Wed, 25 May 2022 08:36:44 GMT

←Older revision		Revision as of 08:36, 25 May 2022
Line 1:		Line 1:

	==Crystal structure of Bs-CspB in complex with r(GUCUUUA)==		==Crystal structure of Bs-CspB in complex with r(GUCUUUA)==
-	<StructureSection load='3pf4' size='340' side='right' caption='[[3pf4]], [[Resolution\|resolution]] 1.38Å' scene=''>	+	<StructureSection load='3pf4' size='340' side='right'caption='[[3pf4]], [[Resolution\|resolution]] 1.38Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3pf4]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"~~bacillus_globigii~~"~~_migula_1900~~ "~~bacillus globigii~~" ~~migula 1900~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PF4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3PF4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3pf4]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PF4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PF4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1csp\|1csp]], [[2hax\|2hax]], [[2es2\|2es2]], [[2f52\|2f52]], [[1c9o\|1c9o]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1csp\|1csp]], [[2hax\|2hax]], [[2es2\|2es2]], [[2f52\|2f52]], [[1c9o\|1c9o]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU09100, cspA, cspB ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "~~Bacillus globigii~~" ~~Migula 1900~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU09100, cspA, cspB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3pf4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pf4 OCA], [~~http~~://pdbe.org/3pf4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3pf4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3pf4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3pf4 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pf4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pf4 OCA], [https://pdbe.org/3pf4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pf4 RCSB], [https://www.ebi.ac.uk/pdbsum/3pf4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pf4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CSPB_BACSU CSPB_BACSU]] Binds to the pentamer sequences ATTGG and CCAAT with highest affinity in single-stranded DNA, and also to other sequences. Has greater affinity for ATTGG than CCAAT. Can act as transcriptional activator of cold shock genes by recognizing putative ATTGG-box elements present in promoter regions of genes induced under cold shock conditions.	+	[[https://www.uniprot.org/uniprot/CSPB_BACSU CSPB_BACSU]] Binds to the pentamer sequences ATTGG and CCAAT with highest affinity in single-stranded DNA, and also to other sequences. Has greater affinity for ATTGG than CCAAT. Can act as transcriptional activator of cold shock genes by recognizing putative ATTGG-box elements present in promoter regions of genes induced under cold shock conditions.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus globigii migula 1900~~]]	+	[[Category: Vibrio subtilis ehrenberg 1835]]
		+	[[Category: Large Structures]]
	[[Category: Heinemann, U]]		[[Category: Heinemann, U]]
	[[Category: Max, K E.A]]		[[Category: Max, K E.A]]

OCA at 10:35, 5 August 2016

OCA — Fri, 05 Aug 2016 10:35:45 GMT

←Older revision		Revision as of 10:35, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Bs-CspB in complex with r(GUCUUUA)==		==Crystal structure of Bs-CspB in complex with r(GUCUUUA)==
	<StructureSection load='3pf4' size='340' side='right' caption='[[3pf4]], [[Resolution\|resolution]] 1.38Å' scene=''>		<StructureSection load='3pf4' size='340' side='right' caption='[[3pf4]], [[Resolution\|resolution]] 1.38Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3pf4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_subtilis Bacillus subtilis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PF4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PF4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3pf4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PF4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PF4 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1csp\|1csp]], [[2hax\|2hax]], [[2es2\|2es2]], [[2f52\|2f52]], [[1c9o\|1c9o]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1csp\|1csp]], [[2hax\|2hax]], [[2es2\|2es2]], [[2f52\|2f52]], [[1c9o\|1c9o]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU09100, cspA, cspB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus ~~subtilis~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU09100, cspA, cspB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Bacillus globigii" Migula 1900])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pf4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pf4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pf4 RCSB], [http://www.ebi.ac.uk/pdbsum/3pf4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pf4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pf4 OCA], [http://pdbe.org/3pf4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3pf4 RCSB], [http://www.ebi.ac.uk/pdbsum/3pf4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3pf4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3pf4" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus ~~subtilis~~]]	+	[[Category: Bacillus globigii migula 1900]]
	[[Category: Heinemann, U]]		[[Category: Heinemann, U]]
	[[Category: Max, K E.A]]		[[Category: Max, K E.A]]

OCA at 01:38, 25 December 2014

OCA — Thu, 25 Dec 2014 01:38:42 GMT

←Older revision		Revision as of 01:38, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3pf4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PF4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PF4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3pf4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PF4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PF4 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1csp\|1csp]], [[2hax\|2hax]], [[2es2\|2es2]], [[2f52\|2f52]], [[1c9o\|1c9o]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1csp\|1csp]], [[2hax\|2hax]], [[2es2\|2es2]], [[2f52\|2f52]], [[1c9o\|1c9o]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU09100, cspA, cspB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU09100, cspA, cspB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pf4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pf4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pf4 RCSB], [http://www.ebi.ac.uk/pdbsum/3pf4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pf4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pf4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pf4 RCSB], [http://www.ebi.ac.uk/pdbsum/3pf4 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CSPB_BACSU CSPB_BACSU]] Binds to the pentamer sequences ATTGG and CCAAT with highest affinity in single-stranded DNA, and also to other sequences. Has greater affinity for ATTGG than CCAAT. Can act as transcriptional activator of cold shock genes by recognizing putative ATTGG-box elements present in promoter regions of genes induced under cold shock conditions.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 16:
	RNA single strands bind to a conserved surface of the major cold shock protein in crystals and solution.,Sachs R, Max KE, Heinemann U, Balbach J RNA. 2011 Nov 29. PMID:22128343<ref>PMID:22128343</ref>		RNA single strands bind to a conserved surface of the major cold shock protein in crystals and solution.,Sachs R, Max KE, Heinemann U, Balbach J RNA. 2011 Nov 29. PMID:22128343<ref>PMID:22128343</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 21:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus subtilis]]		[[Category: Bacillus subtilis]]
-	[[Category: Heinemann, U.]]	+	[[Category: Heinemann, U]]
-	[[Category: Max, K E.A.]]	+	[[Category: Max, K E.A]]
-	[[Category: Sachs, R.]]	+	[[Category: Sachs, R]]
	[[Category: Beta barrel]]		[[Category: Beta barrel]]
	[[Category: Cold shock domain]]		[[Category: Cold shock domain]]

OCA at 05:23, 4 June 2014

OCA — Wed, 04 Jun 2014 05:23:25 GMT

←Older revision		Revision as of 05:23, 4 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3pf4.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of Bs-CspB in complex with r(GUCUUUA)==
		+	<StructureSection load='3pf4' size='340' side='right' caption='[[3pf4]], [[Resolution\|resolution]] 1.38Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3pf4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PF4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PF4 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1csp\|1csp]], [[2hax\|2hax]], [[2es2\|2es2]], [[2f52\|2f52]], [[1c9o\|1c9o]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU09100, cspA, cspB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pf4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pf4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pf4 RCSB], [http://www.ebi.ac.uk/pdbsum/3pf4 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacterial cold shock proteins (CSPs) regulate the cellular response to temperature downshift. Their general principle of function involves RNA chaperoning and transcriptional antitermination. Here we present two crystal structures of cold shock protein B from Bacillus subtilis (Bs-CspB) in complex with either a hexanucleotide (5'-UUUUUU-3') or heptanucleotide (5'-GUCUUUA-3') single-stranded RNA (ssRNA). Hydrogen bonds and stacking interactions between RNA bases and aromatic sidechains characterize individual binding subsites. Additional binding subsites which are not occupied by the ligand in the crystal structure were revealed by NMR spectroscopy in solution on Bs-CspB.RNA complexes. Binding studies demonstrate that Bs-CspB associates with ssDNA as well as ssRNA with moderate sequence specificity. Varying affinities of oligonucleotides are reflected mainly in changes of the dissociation rates. The generally lower binding affinity of ssRNA compared to its ssDNA analog is attributed solely to the substitution of thymine by uracil bases in RNA.

-	~~<!--~~	+	RNA single strands bind to a conserved surface of the major cold shock protein in crystals and solution.,Sachs R, Max KE, Heinemann U, Balbach J RNA. 2011 Nov 29. PMID:22128343<ref>PMID:22128343</ref>
-	~~The line below this paragraph, containing "STRUCTURE_3pf4", creates the "Structure Box" on~~ the ~~page~~.	+
-	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded)~~,	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3pf4\| PDB=3pf4 \| SCENE= }}~~	+

-	~~===Crystal structure~~ of ~~Bs-CspB in complex with r(GUCUUUA)===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22128343}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22128343 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_22128343}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3pf4]] is a 3 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PF4 OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022128343<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Bacillus subtilis]]		[[Category: Bacillus subtilis]]
	[[Category: Heinemann, U.]]		[[Category: Heinemann, U.]]

OCA at 10:40, 14 December 2011

OCA — Wed, 14 Dec 2011 10:40:47 GMT

←Older revision		Revision as of 10:40, 14 December 2011
Line 1:		Line 1:
-	[[Image:3pf4.~~jpg~~\|left\|200px]]	+	[[Image:3pf4.png\|left\|200px]]

	<!--		<!--
Line 11:		Line 11:
	===Crystal structure of Bs-CspB in complex with r(GUCUUUA)===		===Crystal structure of Bs-CspB in complex with r(GUCUUUA)===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22128343}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22128343 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22128343}}

	==About this Structure==		==About this Structure==
	[[3pf4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PF4 OCA].		[[3pf4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PF4 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022128343</ref><references group="xtra"/>
	[[Category: Bacillus subtilis]]		[[Category: Bacillus subtilis]]
	[[Category: Heinemann, U.]]		[[Category: Heinemann, U.]]

OCA: Protected "3pf4" [edit=sysop:move=sysop]

OCA — Wed, 21 Sep 2011 06:08:25 GMT

Protected "3pf4" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:08, 21 September 2011

OCA at 06:08, 21 September 2011

OCA — Wed, 21 Sep 2011 06:08:19 GMT

←Older revision		Revision as of 06:08, 21 September 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3pf4.jpg\|left\|200px]]

-	The ~~entry 3pf4~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3pf4", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3pf4\| PDB=3pf4 \| SCENE= }}

-	~~Authors: Sachs, R., Max, K.E.A., Heinemann, U.~~	+	===Crystal structure of Bs-CspB in complex with r(GUCUUUA)===

-	Description: Crystal structure of Bs-CspB in complex with r(GUCUUUA)

-	~~''Page seeded by~~ [http://oca.weizmann.ac.il/oca OCA ] ~~on Wed Dec 15 08~~:23:~~35 2010''~~	+	==About this Structure==
		+	[[3pf4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PF4 OCA].
		+	[[Category: Bacillus subtilis]]
		+	[[Category: Heinemann, U.]]
		+	[[Category: Max, K E.A.]]
		+	[[Category: Sachs, R.]]
		+	[[Category: Beta barrel]]
		+	[[Category: Cold shock domain]]
		+	[[Category: Cold shock response]]
		+	[[Category: Cytosol]]
		+	[[Category: Gene regulation-rna complex]]
		+	[[Category: Ob fold]]
		+	[[Category: Protein-rna complex]]
		+	[[Category: Rna/dna binding]]
		+	[[Category: Single-stranded rna and dna]]
		+	[[Category: Transcription regulation]]
		+	[[Category: Translation regulation]]

OCA at 05:11, 15 December 2010

OCA — Wed, 15 Dec 2010 05:11:15 GMT

←Older revision		Revision as of 05:11, 15 December 2010
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3pf4 is ON HOLD	+	The entry 3pf4 is ON HOLD until Paper Publication

	Authors: Sachs, R., Max, K.E.A., Heinemann, U.		Authors: Sachs, R., Max, K.E.A., Heinemann, U.
Line 7:		Line 7:
	Description: Crystal structure of Bs-CspB in complex with r(GUCUUUA)		Description: Crystal structure of Bs-CspB in complex with r(GUCUUUA)

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ~~Nov 3 10:~~08:32 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 15 08:23:35 2010''

OCA: New page: '''Unreleased structure''' The entry 3pf4 is ON HOLD Authors: Sachs, R., Max, K.E.A., Heinemann, U. Description: Crystal structure of Bs-CspB in complex with r(GUCUUUA) ''Page seeded ...

OCA — Wed, 03 Nov 2010 06:57:53 GMT

New page: '''Unreleased structure''' The entry 3pf4 is ON HOLD Authors: Sachs, R., Max, K.E.A., Heinemann, U. Description: Crystal structure of Bs-CspB in complex with r(GUCUUUA) ''Page seeded ...

New page

'''Unreleased structure'''

The entry 3pf4 is ON HOLD

Authors: Sachs, R., Max, K.E.A., Heinemann, U.

Description: Crystal structure of Bs-CspB in complex with r(GUCUUUA)

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 3 10:08:32 2010''