3pp5 - Revision history

OCA at 10:42, 21 February 2024

OCA — Wed, 21 Feb 2024 10:42:27 GMT

←Older revision		Revision as of 10:42, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='3pp5' size='340' side='right'caption='[[3pp5]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='3pp5' size='340' side='right'caption='[[3pp5]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3pp5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_11735 Atcc 11735~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PP5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PP5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3pp5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PP5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PP5 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">brk1, DDB_G0279175, hspc300 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 ATCC 11735])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pp5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pp5 OCA], [https://pdbe.org/3pp5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pp5 RCSB], [https://www.ebi.ac.uk/pdbsum/3pp5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pp5 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pp5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pp5 OCA], [https://pdbe.org/3pp5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pp5 RCSB], [https://www.ebi.ac.uk/pdbsum/3pp5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pp5 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/BRK1_DICDI BRK1_DICDI]] Involved in regulation of actin and microtubule organization.	+	[https://www.uniprot.org/uniprot/BRK1_DICDI BRK1_DICDI] Involved in regulation of actin and microtubule organization.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The Scar/WAVE-complex links upstream Rho-GTPase signaling to the activation of the conserved Arp2/3-complex. Scar/WAVE-induced and Arp2/3-complex-mediated actin nucleation is crucial for actin assembly in protruding lamellipodia to drive cell migration. The heteropentameric Scar/WAVE-complex is composed of Scar/WAVE, Abi, Nap, Pir and a small polypeptide Brk1/HSPC300, and recent work suggested that free Brk1 serves as a homooligomeric precursor in the assembly of this complex. Here we characterized the Brk1 trimer from Dictyostelium by analytical ultracentrifugation and gelfiltration. We show for the first time its dissociation at concentrations in the nanomolar range as well as an exchange of subunits within different DdBrk1 containing complexes. Moreover, we determined the three-dimensional structure of DdBrk1 at 1.5 A resolution by X-ray crystallography. Three chains of DdBrk1 are associated with each other forming a parallel triple coiled-coil bundle. Notably, this structure is highly similar to the heterotrimeric alpha-helical bundle of HSPC300/WAVE1/Abi2 within the human Scar/WAVE-complex. This finding, together with the fact that Brk1 is collectively sandwiched by the remaining subunits and also constitutes the main subunit connecting the triple-coil domain of the HSPC300/WAVE1/Abi2/ heterotrimer to Sra1(Pir1), implies a critical function of this subunit in the assembly process of the entire Scar/WAVE-complex.	+
-		+
-	High-resolution X-ray structure of the trimeric Scar/WAVE-complex precursor Brk1.,Linkner J, Witte G, Stradal T, Curth U, Faix J PLoS One. 2011;6(6):e21327. Epub 2011 Jun 20. PMID:21701600<ref>PMID:21701600</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3pp5" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 11735~~]]	+	[[Category: Dictyostelium discoideum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Curth, U]]	+	[[Category: Curth U]]
-	[[Category: Faix, J]]	+	[[Category: Faix J]]
-	[[Category: Linkner, J]]	+	[[Category: Linkner J]]
-	[[Category: Witte, G]]	+	[[Category: Witte G]]
-	~~[[Category: Abi]]~~	+
-	~~[[Category: Precursor of the scar-wave complex]]~~	+
-	~~[[Category: Scar]]~~	+
-	~~[[Category: Structural protein]]~~	+
-	~~[[Category: Triple coiled-coil~~]]	+

OCA at 08:49, 25 May 2022

OCA — Wed, 25 May 2022 08:49:04 GMT

←Older revision		Revision as of 08:49, 25 May 2022
Line 1:		Line 1:

	==High-resolution structure of the trimeric Scar/WAVE complex precursor Brk1==		==High-resolution structure of the trimeric Scar/WAVE complex precursor Brk1==
-	<StructureSection load='3pp5' size='340' side='right' caption='[[3pp5]], [[Resolution\|resolution]] 1.50Å' scene=''>	+	<StructureSection load='3pp5' size='340' side='right'caption='[[3pp5]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3pp5]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_11735 Atcc 11735]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PP5 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3PP5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3pp5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_11735 Atcc 11735]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PP5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PP5 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">brk1, DDB_G0279175, hspc300 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 ATCC 11735])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">brk1, DDB_G0279175, hspc300 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 ATCC 11735])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3pp5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pp5 OCA], [~~http~~://pdbe.org/3pp5 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3pp5 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3pp5 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3pp5 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pp5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pp5 OCA], [https://pdbe.org/3pp5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pp5 RCSB], [https://www.ebi.ac.uk/pdbsum/3pp5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pp5 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/BRK1_DICDI BRK1_DICDI]] Involved in regulation of actin and microtubule organization.	+	[[https://www.uniprot.org/uniprot/BRK1_DICDI BRK1_DICDI]] Involved in regulation of actin and microtubule organization.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 11735]]		[[Category: Atcc 11735]]
		+	[[Category: Large Structures]]
	[[Category: Curth, U]]		[[Category: Curth, U]]
	[[Category: Faix, J]]		[[Category: Faix, J]]

OCA at 09:45, 4 August 2016

OCA — Thu, 04 Aug 2016 09:45:29 GMT

←Older revision		Revision as of 09:45, 4 August 2016
Line 1:		Line 1:
		+
	==High-resolution structure of the trimeric Scar/WAVE complex precursor Brk1==		==High-resolution structure of the trimeric Scar/WAVE complex precursor Brk1==
	<StructureSection load='3pp5' size='340' side='right' caption='[[3pp5]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='3pp5' size='340' side='right' caption='[[3pp5]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3pp5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Dictyostelium_discoideum Dictyostelium discoideum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PP5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PP5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3pp5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_11735 Atcc 11735]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PP5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PP5 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">brk1, DDB_G0279175, hspc300 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 ~~Dictyostelium discoideum~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">brk1, DDB_G0279175, hspc300 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 ATCC 11735])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pp5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pp5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pp5 RCSB], [http://www.ebi.ac.uk/pdbsum/3pp5 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pp5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pp5 OCA], [http://pdbe.org/3pp5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3pp5 RCSB], [http://www.ebi.ac.uk/pdbsum/3pp5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3pp5 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3pp5" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Dictyostelium discoideum~~]]	+	[[Category: Atcc 11735]]
	[[Category: Curth, U]]		[[Category: Curth, U]]
	[[Category: Faix, J]]		[[Category: Faix, J]]

OCA at 08:29, 24 December 2014

OCA — Wed, 24 Dec 2014 08:29:55 GMT

←Older revision		Revision as of 08:29, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3pp5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PP5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PP5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3pp5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PP5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PP5 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">brk1, DDB_G0279175, hspc300 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 Dictyostelium discoideum])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">brk1, DDB_G0279175, hspc300 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 Dictyostelium discoideum])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pp5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pp5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pp5 RCSB], [http://www.ebi.ac.uk/pdbsum/3pp5 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pp5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pp5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pp5 RCSB], [http://www.ebi.ac.uk/pdbsum/3pp5 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BRK1_DICDI BRK1_DICDI]] Involved in regulation of actin and microtubule organization.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 13:		Line 15:
	High-resolution X-ray structure of the trimeric Scar/WAVE-complex precursor Brk1.,Linkner J, Witte G, Stradal T, Curth U, Faix J PLoS One. 2011;6(6):e21327. Epub 2011 Jun 20. PMID:21701600<ref>PMID:21701600</ref>		High-resolution X-ray structure of the trimeric Scar/WAVE-complex precursor Brk1.,Linkner J, Witte G, Stradal T, Curth U, Faix J PLoS One. 2011;6(6):e21327. Epub 2011 Jun 20. PMID:21701600<ref>PMID:21701600</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 20:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Dictyostelium discoideum]]		[[Category: Dictyostelium discoideum]]
-	[[Category: Curth, U.]]	+	[[Category: Curth, U]]
-	[[Category: Faix, J.]]	+	[[Category: Faix, J]]
-	[[Category: Linkner, J.]]	+	[[Category: Linkner, J]]
-	[[Category: Witte, G.]]	+	[[Category: Witte, G]]
	[[Category: Abi]]		[[Category: Abi]]
	[[Category: Precursor of the scar-wave complex]]		[[Category: Precursor of the scar-wave complex]]

OCA at 05:22, 4 June 2014

OCA — Wed, 04 Jun 2014 05:22:32 GMT

←Older revision		Revision as of 05:22, 4 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3pp5.~~png~~\|~~left~~\|~~200px~~]]	+	==High-resolution structure of the trimeric Scar/WAVE complex precursor Brk1==
		+	<StructureSection load='3pp5' size='340' side='right' caption='[[3pp5]], [[Resolution\|resolution]] 1.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3pp5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PP5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PP5 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">brk1, DDB_G0279175, hspc300 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=44689 Dictyostelium discoideum])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pp5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pp5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pp5 RCSB], [http://www.ebi.ac.uk/pdbsum/3pp5 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Scar/WAVE-complex links upstream Rho-GTPase signaling to the activation of the conserved Arp2/3-complex. Scar/WAVE-induced and Arp2/3-complex-mediated actin nucleation is crucial for actin assembly in protruding lamellipodia to drive cell migration. The heteropentameric Scar/WAVE-complex is composed of Scar/WAVE, Abi, Nap, Pir and a small polypeptide Brk1/HSPC300, and recent work suggested that free Brk1 serves as a homooligomeric precursor in the assembly of this complex. Here we characterized the Brk1 trimer from Dictyostelium by analytical ultracentrifugation and gelfiltration. We show for the first time its dissociation at concentrations in the nanomolar range as well as an exchange of subunits within different DdBrk1 containing complexes. Moreover, we determined the three-dimensional structure of DdBrk1 at 1.5 A resolution by X-ray crystallography. Three chains of DdBrk1 are associated with each other forming a parallel triple coiled-coil bundle. Notably, this structure is highly similar to the heterotrimeric alpha-helical bundle of HSPC300/WAVE1/Abi2 within the human Scar/WAVE-complex. This finding, together with the fact that Brk1 is collectively sandwiched by the remaining subunits and also constitutes the main subunit connecting the triple-coil domain of the HSPC300/WAVE1/Abi2/ heterotrimer to Sra1(Pir1), implies a critical function of this subunit in the assembly process of the entire Scar/WAVE-complex.

-	<!--	+	High-resolution X-ray structure of the trimeric Scar/WAVE-complex precursor Brk1.,Linkner J, Witte G, Stradal T, Curth U, Faix J PLoS One. 2011;6(6):e21327. Epub 2011 Jun 20. PMID:21701600<ref>PMID:21701600</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3pp5"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3pp5\| PDB=3pp5 \| SCENE= }}~~	+

-	~~===High-resolution structure of the trimeric Scar~~/~~WAVE complex precursor Brk1===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph~~, ~~{{ABSTRACT_PUBMED_21701600}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 21701600 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_21701600}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3pp5]] is a 1 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PP5 OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:021701600<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Dictyostelium discoideum]]		[[Category: Dictyostelium discoideum]]
	[[Category: Curth, U.]]		[[Category: Curth, U.]]

OCA at 06:25, 28 March 2012

OCA — Wed, 28 Mar 2012 06:25:42 GMT

←Older revision		Revision as of 06:25, 28 March 2012
Line 11:		Line 11:
	===High-resolution structure of the trimeric Scar/WAVE complex precursor Brk1===		===High-resolution structure of the trimeric Scar/WAVE complex precursor Brk1===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21701600}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21701600 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21701600}}

	==About this Structure==		==About this Structure==
	[[3pp5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PP5 OCA].		[[3pp5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PP5 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021701600</ref><references group="xtra"/>
	[[Category: Dictyostelium discoideum]]		[[Category: Dictyostelium discoideum]]
	[[Category: Curth, U.]]		[[Category: Curth, U.]]

OCA at 17:57, 6 July 2011

OCA — Wed, 06 Jul 2011 17:57:02 GMT

←Older revision		Revision as of 17:57, 6 July 2011
Line 1:		Line 1:
-	[[Image:3pp5.~~jpg~~\|left\|200px]]	+	[[Image:3pp5.png\|left\|200px]]

	<!--		<!--
Line 19:		Line 19:
	[[Category: Linkner, J.]]		[[Category: Linkner, J.]]
	[[Category: Witte, G.]]		[[Category: Witte, G.]]
		+	[[Category: Abi]]
		+	[[Category: Precursor of the scar-wave complex]]
		+	[[Category: Scar]]
		+	[[Category: Structural protein]]
		+	[[Category: Triple coiled-coil]]

OCA: Protected "3pp5" [edit=sysop:move=sysop]

OCA — Wed, 08 Jun 2011 05:22:01 GMT

Protected "3pp5" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:22, 8 June 2011

OCA at 05:21, 8 June 2011

OCA — Wed, 08 Jun 2011 05:21:55 GMT

←Older revision		Revision as of 05:21, 8 June 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3pp5.jpg\|left\|200px]]

-	The ~~entry 3pp5~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3pp5", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3pp5\| PDB=3pp5 \| SCENE= }}

-	~~Authors: Linkner, J., Witte, G., Curth, U., Faix, J.~~	+	===High-resolution structure of the trimeric Scar/WAVE complex precursor Brk1===

-	Description: High-resolution structure of the trimeric Scar/WAVE complex precursor Brk1

-	~~''Page seeded by~~ [http://oca.weizmann.ac.il/oca OCA ] ~~on Wed Dec 15 08~~:24:~~38 2010''~~	+	==About this Structure==
		+	[[3pp5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PP5 OCA].
		+	[[Category: Dictyostelium discoideum]]
		+	[[Category: Curth, U.]]
		+	[[Category: Faix, J.]]
		+	[[Category: Linkner, J.]]
		+	[[Category: Witte, G.]]

OCA at 05:12, 15 December 2010

OCA — Wed, 15 Dec 2010 05:12:18 GMT

←Older revision		Revision as of 05:12, 15 December 2010
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3pp5 is ON HOLD	+	The entry 3pp5 is ON HOLD until Paper Publication

	Authors: Linkner, J., Witte, G., Curth, U., Faix, J.		Authors: Linkner, J., Witte, G., Curth, U., Faix, J.
Line 7:		Line 7:
	Description: High-resolution structure of the trimeric Scar/WAVE complex precursor Brk1		Description: High-resolution structure of the trimeric Scar/WAVE complex precursor Brk1

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec ~~1 11:~~15:02 2010''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Dec 15 08:24:38 2010''