3pwa - Revision history

OCA at 13:34, 1 March 2024

OCA — Fri, 01 Mar 2024 13:34:03 GMT

←Older revision		Revision as of 13:34, 1 March 2024
Line 3:		Line 3:
	<StructureSection load='3pwa' size='340' side='right'caption='[[3pwa]], [[Resolution\|resolution]] 2.04Å' scene=''>		<StructureSection load='3pwa' size='340' side='right'caption='[[3pwa]], [[Resolution\|resolution]] 2.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3pwa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Plafa Plafa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PWA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PWA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3pwa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PWA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PWA FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2~~-~~ETHANEDIOL'>EDO</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.04Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[3pvf\|3pvf]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~TPI ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=5833 PLAFA])</td~~></tr>	+
-	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pwa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pwa OCA], [https://pdbe.org/3pwa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pwa RCSB], [https://www.ebi.ac.uk/pdbsum/3pwa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pwa ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pwa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pwa OCA], [https://pdbe.org/3pwa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pwa RCSB], [https://www.ebi.ac.uk/pdbsum/3pwa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pwa ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/TPIS_PLAFA TPIS_PLAFA]
-	Cys 126 is a completely conserved residue in triosephosphate isomerase, which is proximal to the active site, but has been ascribed no specific role in catalysis. A previous study of the C126S and C126A mutants of yeast TIM reported substantial catalytic activity for the mutant enzymes leading to suggest that this residue is implicated in folding and stability [Gonzalez-Mondragon et al, (2004) Biochemistry 43, 3255-3263]. We have re-examined the role of Cys 126 using the Plasmodium falciparum enzyme as a model. Five mutants, C126S, C126A, C126V, C126M and C126T have been characterized. Crystal structures of 3-phosphoglycolate (PGA) bound C126S mutant and the unliganded forms of the C126S and C126A mutants have been determined at a resolution of 1.7 A to 2.1 A. Kinetic studies reveal a approximately 5 fold drop in k(cat) for C126S and C126A mutants, while a approximately 10 fold drop is observed for the other three mutants. At ambient temperature, the wild type enzyme and all five mutants show no concentration dependence of activity. At higher temperatures (> 40 degrees C) the mutant enzymes show a significant concentration dependence, with a dramatic loss in activity below 15 muM. The mutant enzymes also have diminished thermal stability at low concentration as monitored by far UV circular dichroism. These results suggest that Cys 126 contributes to the stability of the dimer interface through a network of interactions involving His 95, Glu 97 and Arg 98, which form direct contacts across the dimer interface. Structured digital abstract: ~~Tim binds to Tim by x-ray crystallography (View interaction).~~	+
-		+
-	Probing the Role of the Fully Conserved Cys 126 Residue in Triosephosphate Isomerase by Site Specific Mutagenesis. Distal Effects on Dimer Stability.,Samanta M, Banerjee M, Murthy MR, Balaram H, Balaram P FEBS J. 2011 Mar 29. doi: 10.1111/~~j.1742-4658.2011.08110.x. PMID:21447068<ref>PMID:21447068</ref>~~	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3pwa" style="background-color:#fffaf0;"><~~/~~div>~~	+

	==See Also==		==See Also==
	*[[Triose phosphate isomerase 3D structures\|Triose phosphate isomerase 3D structures]]		*[[Triose phosphate isomerase 3D structures\|Triose phosphate isomerase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Plafa]]~~	+	[[Category: Plasmodium falciparum]]
-	~~[[Category: Triose-phosphate isomerase~~]]	+	[[Category: Balaram H]]
-	[[Category: Balaram, H]]	+	[[Category: Balaram P]]
-	[[Category: Balaram, P]]	+	[[Category: Banerjee M]]
-	[[Category: Banerjee, M]]	+	[[Category: Murthy MRN]]
-	[[Category: Murthy~~, M R.N~~]]	+	[[Category: Samanta M]]
-	[[Category: Samanta, M]]	+
-	~~[[Category: Isomerase]]~~	+
-	~~[[Category: Tim barrel~~]]	+

OCA at 08:58, 25 May 2022

OCA — Wed, 25 May 2022 08:58:50 GMT

←Older revision		Revision as of 08:58, 25 May 2022
Line 1:		Line 1:

	==Structure of C126A mutant of Plasmodium falciparum triosephosphate isomerase==		==Structure of C126A mutant of Plasmodium falciparum triosephosphate isomerase==
-	<StructureSection load='3pwa' size='340' side='right' caption='[[3pwa]], [[Resolution\|resolution]] 2.04Å' scene=''>	+	<StructureSection load='3pwa' size='340' side='right'caption='[[3pwa]], [[Resolution\|resolution]] 2.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3pwa]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Plafa Plafa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PWA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3PWA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3pwa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Plafa Plafa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PWA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PWA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3pvf\|3pvf]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3pvf\|3pvf]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TPI ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5833 PLAFA])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TPI ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5833 PLAFA])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3pwa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pwa OCA], [~~http~~://pdbe.org/3pwa PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3pwa RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3pwa PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3pwa ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pwa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pwa OCA], [https://pdbe.org/3pwa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pwa RCSB], [https://www.ebi.ac.uk/pdbsum/3pwa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pwa ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 21:

	==See Also==		==See Also==
-	*[[Triose ~~Phosphate Isomerase~~\|Triose ~~Phosphate Isomerase~~]]	+	*[[Triose phosphate isomerase 3D structures\|Triose phosphate isomerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Plafa]]		[[Category: Plafa]]
	[[Category: Triose-phosphate isomerase]]		[[Category: Triose-phosphate isomerase]]

OCA at 10:29, 4 August 2016

OCA — Thu, 04 Aug 2016 10:29:34 GMT

←Older revision		Revision as of 10:29, 4 August 2016
Line 1:		Line 1:
		+
	==Structure of C126A mutant of Plasmodium falciparum triosephosphate isomerase==		==Structure of C126A mutant of Plasmodium falciparum triosephosphate isomerase==
	<StructureSection load='3pwa' size='340' side='right' caption='[[3pwa]], [[Resolution\|resolution]] 2.04Å' scene=''>		<StructureSection load='3pwa' size='340' side='right' caption='[[3pwa]], [[Resolution\|resolution]] 2.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3pwa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Plasmodium_falciparum Plasmodium falciparum~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PWA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PWA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3pwa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Plafa Plafa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PWA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PWA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3pvf\|3pvf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3pvf\|3pvf]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TPI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5833 ~~Plasmodium falciparum~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TPI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5833 PLAFA])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pwa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pwa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pwa RCSB], [http://www.ebi.ac.uk/pdbsum/3pwa PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pwa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pwa OCA], [http://pdbe.org/3pwa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3pwa RCSB], [http://www.ebi.ac.uk/pdbsum/3pwa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3pwa ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3pwa" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 24:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Plasmodium falciparum~~]]	+	[[Category: Plafa]]
	[[Category: Triose-phosphate isomerase]]		[[Category: Triose-phosphate isomerase]]
	[[Category: Balaram, H]]		[[Category: Balaram, H]]

OCA at 10:49, 19 December 2014

OCA — Fri, 19 Dec 2014 10:49:52 GMT

←Older revision		Revision as of 10:49, 19 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3pwa~~\| ~~PDB~~=3pwa \| ~~SCENE~~= }}	+	==Structure of C126A mutant of Plasmodium falciparum triosephosphate isomerase==
-	===~~Structure of C126A mutant of~~ Plasmodium falciparum ~~triosephosphate~~ isomerase===	+	<StructureSection load='3pwa' size='340' side='right' caption='[[3pwa]], [[Resolution\|resolution]] 2.04Å' scene=''>
-	~~{{ABSTRACT_PUBMED_21447068}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3pwa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PWA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PWA FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3pvf\|3pvf]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TPI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5833 Plasmodium falciparum])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pwa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pwa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pwa RCSB], [http://www.ebi.ac.uk/pdbsum/3pwa PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cys 126 is a completely conserved residue in triosephosphate isomerase, which is proximal to the active site, but has been ascribed no specific role in catalysis. A previous study of the C126S and C126A mutants of yeast TIM reported substantial catalytic activity for the mutant enzymes leading to suggest that this residue is implicated in folding and stability [Gonzalez-Mondragon et al, (2004) Biochemistry 43, 3255-3263]. We have re-examined the role of Cys 126 using the Plasmodium falciparum enzyme as a model. Five mutants, C126S, C126A, C126V, C126M and C126T have been characterized. Crystal structures of 3-phosphoglycolate (PGA) bound C126S mutant and the unliganded forms of the C126S and C126A mutants have been determined at a resolution of 1.7 A to 2.1 A. Kinetic studies reveal a approximately 5 fold drop in k(cat) for C126S and C126A mutants, while a approximately 10 fold drop is observed for the other three mutants. At ambient temperature, the wild type enzyme and all five mutants show no concentration dependence of activity. At higher temperatures (> 40 degrees C) the mutant enzymes show a significant concentration dependence, with a dramatic loss in activity below 15 muM. The mutant enzymes also have diminished thermal stability at low concentration as monitored by far UV circular dichroism. These results suggest that Cys 126 contributes to the stability of the dimer interface through a network of interactions involving His 95, Glu 97 and Arg 98, which form direct contacts across the dimer interface. Structured digital abstract: Tim binds to Tim by x-ray crystallography (View interaction).

-	~~==About this Structure==~~	+	Probing the Role of the Fully Conserved Cys 126 Residue in Triosephosphate Isomerase by Site Specific Mutagenesis. Distal Effects on Dimer Stability.,Samanta M, Banerjee M, Murthy MR, Balaram H, Balaram P FEBS J. 2011 Mar 29. doi: 10.1111/j.1742-4658.2011.08110.x. PMID:21447068<ref>PMID:21447068</ref>
-	~~[[3pwa]] is a 2 chain structure with sequence from [http~~:/~~/en~~.~~wikipedia~~.~~org/wiki/Plasmodium_falciparum Plasmodium falciparum]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=3PWA OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[Triose Phosphate Isomerase\|Triose Phosphate Isomerase]]		*[[Triose Phosphate Isomerase\|Triose Phosphate Isomerase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:021447068</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Plasmodium falciparum]]		[[Category: Plasmodium falciparum]]
	[[Category: Triose-phosphate isomerase]]		[[Category: Triose-phosphate isomerase]]
-	[[Category: Balaram, H.]]	+	[[Category: Balaram, H]]
-	[[Category: Balaram, P.]]	+	[[Category: Balaram, P]]
-	[[Category: Banerjee, M.]]	+	[[Category: Banerjee, M]]
-	[[Category: Murthy, M R.N.]]	+	[[Category: Murthy, M R.N]]
-	[[Category: Samanta, M.]]	+	[[Category: Samanta, M]]
	[[Category: Isomerase]]		[[Category: Isomerase]]
	[[Category: Tim barrel]]		[[Category: Tim barrel]]

OCA at 05:15, 28 August 2013

OCA — Wed, 28 Aug 2013 05:15:49 GMT

←Older revision		Revision as of 05:15, 28 August 2013
Line 10:		Line 10:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:021447068</ref><references group="xtra"/>	+	<ref group="xtra">PMID:021447068</ref><references group="xtra"/><references/>
	[[Category: Plasmodium falciparum]]		[[Category: Plasmodium falciparum]]
	[[Category: Triose-phosphate isomerase]]		[[Category: Triose-phosphate isomerase]]

OCA at 10:32, 11 March 2013

OCA — Mon, 11 Mar 2013 10:32:50 GMT

←Older revision		Revision as of 10:32, 11 March 2013
Line 1:		Line 1:
-	[[Image:3pwa.png\|left\|200px]]
-
	{{STRUCTURE_3pwa\| PDB=3pwa \| SCENE= }}		{{STRUCTURE_3pwa\| PDB=3pwa \| SCENE= }}
-
	===Structure of C126A mutant of Plasmodium falciparum triosephosphate isomerase===		===Structure of C126A mutant of Plasmodium falciparum triosephosphate isomerase===
-
	{{ABSTRACT_PUBMED_21447068}}		{{ABSTRACT_PUBMED_21447068}}

OCA at 05:58, 20 October 2012

OCA — Sat, 20 Oct 2012 05:58:30 GMT

←Older revision		Revision as of 05:58, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3pwa]] is a 2 chain structure ~~of [[Triose Phosphate Isomerase]]~~ with sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PWA OCA].	+	[[3pwa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PWA OCA].

	==See Also==		==See Also==

OCA at 02:47, 29 August 2012

OCA — Wed, 29 Aug 2012 02:47:13 GMT

←Older revision		Revision as of 02:47, 29 August 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3pwa]] is a 2 chain structure of ~~[[TIM test]] and~~ [[Triose Phosphate Isomerase]] with sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PWA OCA].	+	[[3pwa]] is a 2 chain structure of [[Triose Phosphate Isomerase]] with sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PWA OCA].

	==See Also==		==See Also==
-	*[[TIM test\|TIM test]]
	*[[Triose Phosphate Isomerase\|Triose Phosphate Isomerase]]		*[[Triose Phosphate Isomerase\|Triose Phosphate Isomerase]]

OCA at 11:42, 25 July 2012

OCA — Wed, 25 Jul 2012 11:42:01 GMT

←Older revision		Revision as of 11:42, 25 July 2012
Line 1:		Line 1:
-	[[Image:3pwa.~~jpg~~\|left\|200px]]	+	[[Image:3pwa.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3pwa", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3pwa\| PDB=3pwa \| SCENE= }}		{{STRUCTURE_3pwa\| PDB=3pwa \| SCENE= }}

	===Structure of C126A mutant of Plasmodium falciparum triosephosphate isomerase===		===Structure of C126A mutant of Plasmodium falciparum triosephosphate isomerase===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21447068}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 21447068 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_21447068}}		{{ABSTRACT_PUBMED_21447068}}

	==About this Structure==		==About this Structure==
-	[[3pwa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PWA OCA].	+	[[3pwa]] is a 2 chain structure of [[TIM test]] and [[Triose Phosphate Isomerase]] with sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PWA OCA].
		+
		+	==See Also==
		+	*[[TIM test\|TIM test]]
		+	*[[Triose Phosphate Isomerase\|Triose Phosphate Isomerase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~21447068~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:021447068</ref><references group="xtra"/>
	[[Category: Plasmodium falciparum]]		[[Category: Plasmodium falciparum]]
	[[Category: Triose-phosphate isomerase]]		[[Category: Triose-phosphate isomerase]]
Line 30:		Line 23:
	[[Category: Murthy, M R.N.]]		[[Category: Murthy, M R.N.]]
	[[Category: Samanta, M.]]		[[Category: Samanta, M.]]
		+	[[Category: Isomerase]]
		+	[[Category: Tim barrel]]

OCA at 07:15, 27 April 2011

OCA — Wed, 27 Apr 2011 07:15:34 GMT

←Older revision		Revision as of 07:15, 27 April 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3pwa.jpg\|left\|200px]]

-	The ~~entry 3pwa~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3pwa", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3pwa\| PDB=3pwa \| SCENE= }}

-	~~Authors: Samanta, M., Banerjee, M., Murthy, M.R.N., Balaram, H., Balaram, P.~~	+	===Structure of C126A mutant of Plasmodium falciparum triosephosphate isomerase===

-	~~Description~~: Structure ~~of C126A mutant of~~ Plasmodium falciparum ~~triosephosphate~~ isomerase	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21447068}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21447068 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21447068}}
		+
		+	==About this Structure==
		+	[[3pwa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PWA OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:21447068</ref><references group="xtra"/>
		+	[[Category: Plasmodium falciparum]]
		+	[[Category: Triose-phosphate isomerase]]
		+	[[Category: Balaram, H.]]
		+	[[Category: Balaram, P.]]
		+	[[Category: Banerjee, M.]]
		+	[[Category: Murthy, M R.N.]]
		+	[[Category: Samanta, M.]]