3pzu - Revision history

OCA at 10:44, 21 February 2024

OCA — Wed, 21 Feb 2024 10:44:45 GMT

←Older revision		Revision as of 10:44, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='3pzu' size='340' side='right'caption='[[3pzu]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3pzu' size='340' side='right'caption='[[3pzu]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3pzu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bacsu Bacsu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PZU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PZU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3pzu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PZU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PZU FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max~~-~~height: 3em;'>[[3pzt\|3pzt]]~~, [[~~3pzv~~\|~~3pzv~~]]~~</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>eglS, bglC, gld, BSU18130 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~224308 BACSU])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pzu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pzu OCA], [https://pdbe.org/3pzu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pzu RCSB], [https://www.ebi.ac.uk/pdbsum/3pzu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pzu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pzu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pzu OCA], [https://pdbe.org/3pzu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pzu RCSB], [https://www.ebi.ac.uk/pdbsum/3pzu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pzu ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/GUN2_BACSU GUN2_BACSU]
-	Cellulases participate in a number of biological events such as plant cell wall remodeling, nematode parasitism and microbial carbon uptake. Their ability to depolymerize crystalline cellulose is of great biotechnological interest for environmentally-compatible production of fuels from lignocellulosic biomass. However, industrial use of cellulases is somewhat limited both by their low catalytic efficiency and stability. In this work, we conducted a detailed functional and structural characterization of the thermostable cellulase 5A from Bacillus subtilis (BsCel5A), which consists of a GH5 catalytic domain fused to a family 3 carbohydrate-binding module (CBM3). NMR structural analysis revealed that the Bacillus CBM3 represents a new subfamily, which lacks the classical calcium-binding motif and variations in NMR frequencies in the presence of cellopentaose showed the importance of polar residues in the carbohydrate interaction. Together with the catalytic domain, the CBM3 forms a large planar surface for cellulose recognition, which conducts the substrate in a proper conformation to the active site and increases enzymatic efficiency. Notably, the manganese ion demonstrated to have a hyper-stabilizing effect on BsCel5A and by using deletion constructs and X-ray crystallography, we determined that this effect maps to a negatively charged motif located at the opposite face of the catalytic site.	+
-		+
-	Dissecting structure-function-stability relationships of a thermostable GH5-CBM3 cellulase from Bacillus subtilis 168.,Santos C, Paiva J, Sforca M, Neves J, Navarro R, Cota J, Akao P, Hoffmam ZB, Meza A, Smetana J, Nogueira M, Polikarpov I, Xavier-Neto J, Squina F, Ward RJ, Ruller R, Zeri A, Murakami MT Biochem J. 2011 Sep 1. PMID:~~21880019<ref>PMID:21880019<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3pzu" style="background-color:#fffaf0;"><~~/~~div>~~	+

	==See Also==		==See Also==
	*[[Glucanase 3D structures\|Glucanase 3D structures]]		*[[Glucanase 3D structures\|Glucanase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacsu]]~~	+	[[Category: Bacillus subtilis subsp. subtilis str. 168]]
-	~~[[Category: Cellulase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Akao~~, P K~~]]	+	[[Category: Akao PK]]
-	[[Category: Meza~~, A N~~]]	+	[[Category: Meza AN]]
-	[[Category: Murakami~~, M T~~]]	+	[[Category: Murakami MT]]
-	[[Category: Paiva~~, J H~~]]	+	[[Category: Paiva JH]]
-	[[Category: Ruller, R]]	+	[[Category: Ruller R]]
-	[[Category: Santos~~, C R~~]]	+	[[Category: Santos CR]]
-	[[Category: Silva~~, J C~~]]	+	[[Category: Silva JC]]
-	[[Category: Squina~~, F M~~]]	+	[[Category: Squina FM]]
-	[[Category: Ward~~, R J]]~~	+	[[Category: Ward RJ]]
-	~~[[Category: Alpha/beta barrel]]~~	+
-	~~[[Category: Cellulose binding]]~~	+
-	~~[[Category: Glycosyl hydrolase]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 09:03, 25 May 2022

OCA — Wed, 25 May 2022 09:03:30 GMT

←Older revision		Revision as of 09:03, 25 May 2022
Line 1:		Line 1:

	==P212121 crystal form of the endo-1,4-beta-glucanase from Bacillus subtilis 168==		==P212121 crystal form of the endo-1,4-beta-glucanase from Bacillus subtilis 168==
-	<StructureSection load='3pzu' size='340' side='right' caption='[[3pzu]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='3pzu' size='340' side='right'caption='[[3pzu]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3pzu]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PZU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3PZU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3pzu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PZU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PZU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3pzt\|3pzt]], [[3pzv\|3pzv]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3pzt\|3pzt]], [[3pzv\|3pzv]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eglS, bglC, gld, BSU18130 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eglS, bglC, gld, BSU18130 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3pzu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pzu OCA], [~~http~~://pdbe.org/3pzu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3pzu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3pzu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3pzu ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pzu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pzu OCA], [https://pdbe.org/3pzu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pzu RCSB], [https://www.ebi.ac.uk/pdbsum/3pzu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pzu ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 3pzu" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3pzu" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glucanase 3D structures\|Glucanase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 25:		Line 28:
	[[Category: Bacsu]]		[[Category: Bacsu]]
	[[Category: Cellulase]]		[[Category: Cellulase]]
		+	[[Category: Large Structures]]
	[[Category: Akao, P K]]		[[Category: Akao, P K]]
	[[Category: Meza, A N]]		[[Category: Meza, A N]]

OCA at 07:45, 15 November 2017

OCA — Wed, 15 Nov 2017 07:45:59 GMT

←Older revision		Revision as of 07:45, 15 November 2017
Line 5:		Line 5:
	<table><tr><td colspan='2'>[[3pzu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PZU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PZU FirstGlance]. <br>		<table><tr><td colspan='2'>[[3pzu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PZU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PZU FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3pzv\|3pzv]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3pzt\|3pzt]], [[3pzv\|3pzv]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eglS, bglC, gld, BSU18130 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eglS, bglC, gld, BSU18130 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 3pzu" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3pzu" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Glucanase\|Glucanase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:22, 10 December 2016

OCA — Sat, 10 Dec 2016 11:22:49 GMT

←Older revision		Revision as of 11:22, 10 December 2016
Line 1:		Line 1:
		+
	==P212121 crystal form of the endo-1,4-beta-glucanase from Bacillus subtilis 168==		==P212121 crystal form of the endo-1,4-beta-glucanase from Bacillus subtilis 168==
	<StructureSection load='3pzu' size='340' side='right' caption='[[3pzu]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3pzu' size='340' side='right' caption='[[3pzu]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3pzu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_subtilis_subsp._subtilis Bacillus subtilis subsp. subtilis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PZU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PZU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3pzu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PZU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PZU FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3pzv\|3pzv]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3pzv\|3pzv]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eglS, bglC, gld, BSU18130 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~135461 Bacillus subtilis subsp. subtilis~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eglS, bglC, gld, BSU18130 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pzu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pzu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pzu RCSB], [http://www.ebi.ac.uk/pdbsum/3pzu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pzu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pzu OCA], [http://pdbe.org/3pzu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3pzu RCSB], [http://www.ebi.ac.uk/pdbsum/3pzu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3pzu ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3pzu" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 24:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus subtilis subsp. subtilis~~]]	+	[[Category: Bacsu]]
	[[Category: Cellulase]]		[[Category: Cellulase]]
	[[Category: Akao, P K]]		[[Category: Akao, P K]]

OCA at 11:01, 9 December 2014

OCA — Tue, 09 Dec 2014 11:01:42 GMT

←Older revision		Revision as of 11:01, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3pzu.~~png~~\|~~left~~\|~~200px~~]]	+	==P212121 crystal form of the endo-1,4-beta-glucanase from Bacillus subtilis 168==
		+	<StructureSection load='3pzu' size='340' side='right' caption='[[3pzu]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3pzu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis Bacillus subtilis subsp. subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PZU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PZU FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3pzv\|3pzv]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">eglS, bglC, gld, BSU18130 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=135461 Bacillus subtilis subsp. subtilis])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pzu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pzu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pzu RCSB], [http://www.ebi.ac.uk/pdbsum/3pzu PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cellulases participate in a number of biological events such as plant cell wall remodeling, nematode parasitism and microbial carbon uptake. Their ability to depolymerize crystalline cellulose is of great biotechnological interest for environmentally-compatible production of fuels from lignocellulosic biomass. However, industrial use of cellulases is somewhat limited both by their low catalytic efficiency and stability. In this work, we conducted a detailed functional and structural characterization of the thermostable cellulase 5A from Bacillus subtilis (BsCel5A), which consists of a GH5 catalytic domain fused to a family 3 carbohydrate-binding module (CBM3). NMR structural analysis revealed that the Bacillus CBM3 represents a new subfamily, which lacks the classical calcium-binding motif and variations in NMR frequencies in the presence of cellopentaose showed the importance of polar residues in the carbohydrate interaction. Together with the catalytic domain, the CBM3 forms a large planar surface for cellulose recognition, which conducts the substrate in a proper conformation to the active site and increases enzymatic efficiency. Notably, the manganese ion demonstrated to have a hyper-stabilizing effect on BsCel5A and by using deletion constructs and X-ray crystallography, we determined that this effect maps to a negatively charged motif located at the opposite face of the catalytic site.

-	~~{{STRUCTURE_3pzu\| PDB=3pzu \| SCENE= }}~~	+	Dissecting structure-function-stability relationships of a thermostable GH5-CBM3 cellulase from Bacillus subtilis 168.,Santos C, Paiva J, Sforca M, Neves J, Navarro R, Cota J, Akao P, Hoffmam ZB, Meza A, Smetana J, Nogueira M, Polikarpov I, Xavier-Neto J, Squina F, Ward RJ, Ruller R, Zeri A, Murakami MT Biochem J. 2011 Sep 1. PMID:21880019<ref>PMID:21880019</ref>

-	~~===P212121 crystal form~~ of the ~~endo-1,4-beta-glucanase from Bacillus subtilis 168===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_21880019}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3pzu]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bacillus_subtilis_subsp~~.~~_subtilis Bacillus subtilis subsp. subtilis]. Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3PZU OCA].~~	+

	==See Also==		==See Also==
	*[[Glucanase\|Glucanase]]		*[[Glucanase\|Glucanase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:021880019<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bacillus subtilis subsp. subtilis]]		[[Category: Bacillus subtilis subsp. subtilis]]
	[[Category: Cellulase]]		[[Category: Cellulase]]
-	[[Category: Akao, P K.]]	+	[[Category: Akao, P K]]
-	[[Category: Meza, A N.]]	+	[[Category: Meza, A N]]
-	[[Category: Murakami, M T.]]	+	[[Category: Murakami, M T]]
-	[[Category: Paiva, J H.]]	+	[[Category: Paiva, J H]]
-	[[Category: Ruller, R.]]	+	[[Category: Ruller, R]]
-	[[Category: Santos, C R.]]	+	[[Category: Santos, C R]]
-	[[Category: Silva, J C.]]	+	[[Category: Silva, J C]]
-	[[Category: Squina, F M.]]	+	[[Category: Squina, F M]]
-	[[Category: Ward, R J.]]	+	[[Category: Ward, R J]]
	[[Category: Alpha/beta barrel]]		[[Category: Alpha/beta barrel]]
	[[Category: Cellulose binding]]		[[Category: Cellulose binding]]
	[[Category: Glycosyl hydrolase]]		[[Category: Glycosyl hydrolase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 15:31, 20 October 2012

OCA — Sat, 20 Oct 2012 15:31:05 GMT

←Older revision		Revision as of 15:31, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3pzu]] is a 2 chain structure ~~of [[Glucanase]]~~ with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis Bacillus subtilis subsp. subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PZU OCA].	+	[[3pzu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis Bacillus subtilis subsp. subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PZU OCA].

	==See Also==		==See Also==

OCA at 18:37, 26 July 2012

OCA — Thu, 26 Jul 2012 18:37:46 GMT

←Older revision		Revision as of 18:37, 26 July 2012
Line 1:		Line 1:
	[[Image:3pzu.png\|left\|200px]]		[[Image:3pzu.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3pzu", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3pzu\| PDB=3pzu \| SCENE= }}		{{STRUCTURE_3pzu\| PDB=3pzu \| SCENE= }}

	===P212121 crystal form of the endo-1,4-beta-glucanase from Bacillus subtilis 168===		===P212121 crystal form of the endo-1,4-beta-glucanase from Bacillus subtilis 168===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21880019}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 21880019 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_21880019}}		{{ABSTRACT_PUBMED_21880019}}

Line 22:		Line 11:

	==See Also==		==See Also==
-	*[[Glucanase]]	+	*[[Glucanase\|Glucanase]]

	==Reference==		==Reference==

OCA at 05:58, 28 December 2011

OCA — Wed, 28 Dec 2011 05:58:34 GMT

←Older revision		Revision as of 05:58, 28 December 2011
Line 1:		Line 1:
-	[[Image:3pzu.~~jpg~~\|left\|200px]]	+	[[Image:3pzu.png\|left\|200px]]

	<!--		<!--
Line 19:		Line 19:

	==About this Structure==		==About this Structure==
-	[[3pzu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis Bacillus subtilis subsp. subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PZU OCA].	+	[[3pzu]] is a 2 chain structure of [[Glucanase]] with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis Bacillus subtilis subsp. subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PZU OCA].
		+
		+	==See Also==
		+	*[[Glucanase]]

	==Reference==		==Reference==

OCA at 06:29, 14 September 2011

OCA — Wed, 14 Sep 2011 06:29:05 GMT

←Older revision		Revision as of 06:29, 14 September 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3pzu.jpg\|left\|200px]]

-	The ~~entry 3pzu~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3pzu", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3pzu\| PDB=3pzu \| SCENE= }}

-	~~Authors: Santos~~, ~~C.R., Paiva, J.H., Akao, P.K., Meza, A.N., Silva, J.C., Squina, F.M., Ward, R.J., Ruller, R., Murakami, M.T.~~	+	===P212121 crystal form of the endo-1,4-beta-glucanase from Bacillus subtilis 168===

-	~~Description~~: ~~P212121 crystal form of~~ the ~~endo~~-~~1,4~~-~~beta-glucanase~~ from Bacillus subtilis ~~168~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21880019}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21880019 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21880019}}
		+
		+	==About this Structure==
		+	[[3pzu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis Bacillus subtilis subsp. subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PZU OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021880019</ref><references group="xtra"/>
		+	[[Category: Bacillus subtilis subsp. subtilis]]
		+	[[Category: Cellulase]]
		+	[[Category: Akao, P K.]]
		+	[[Category: Meza, A N.]]
		+	[[Category: Murakami, M T.]]
		+	[[Category: Paiva, J H.]]
		+	[[Category: Ruller, R.]]
		+	[[Category: Santos, C R.]]
		+	[[Category: Silva, J C.]]
		+	[[Category: Squina, F M.]]
		+	[[Category: Ward, R J.]]
		+	[[Category: Alpha/beta barrel]]
		+	[[Category: Cellulose binding]]
		+	[[Category: Glycosyl hydrolase]]
		+	[[Category: Hydrolase]]

OCA: Protected "3pzu" [edit=sysop:move=sysop]

OCA — Wed, 12 Jan 2011 04:49:48 GMT

Protected "3pzu" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:49, 12 January 2011