3q15 - Revision history

OCA at 10:45, 21 February 2024

OCA — Wed, 21 Feb 2024 10:45:18 GMT

←Older revision		Revision as of 10:45, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='3q15' size='340' side='right'caption='[[3q15]], [[Resolution\|resolution]] 2.19Å' scene=''>		<StructureSection load='3q15' size='340' side='right'caption='[[3q15]], [[Resolution\|resolution]] 2.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3q15]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"vibrio_subtilis"_ehrenberg_1835 "vibrio~~ subtilis~~" ehrenberg 1835~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q15 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3Q15 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3q15]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q15 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3Q15 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.192Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU06830, rapH, yeeH, yzqA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835]), BSU37130, spo0F ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3q15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q15 OCA], [https://pdbe.org/3q15 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3q15 RCSB], [https://www.ebi.ac.uk/pdbsum/3q15 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3q15 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3q15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q15 OCA], [https://pdbe.org/3q15 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3q15 RCSB], [https://www.ebi.ac.uk/pdbsum/3q15 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3q15 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~SP0F_BACSU SP0F_BACSU~~]~~] Key element in the phosphorelay regulating sporulation initiation. Phosphorylation of spo0B during sporulation initiation.~~	+	[https://www.uniprot.org/uniprot/RAPH_BACSU RAPH_BACSU]
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Bacterial Rap family proteins have been most extensively studied in Bacillus subtilis, where they regulate activities including sporulation, genetic competence, antibiotic expression, and the movement of the ICEBs1 transposon. One subset of Rap proteins consists of phosphatases that control B. subtilis and B. anthracis sporulation by dephosphorylating the response regulator Spo0F. The mechanistic basis of Rap phosphatase activity was unknown. Here we present the RapH-Spo0F X-ray crystal structure, which shows that Rap proteins consist of a 3-helix bundle and a tetratricopeptide repeat domain. Extensive biochemical and genetic functional studies reveal the importance of the observed RapH-Spo0F interactions, including the catalytic role of a glutamine in the RapH 3-helix bundle that inserts into the Spo0F active site. We show that in addition to dephosphorylating Spo0F, RapH can antagonize sporulation by sterically blocking phosphoryl transfer to and from Spo0F. Our structure-function analysis of the RapH-Spo0F interaction identified Rap protein residues critical for Spo0F phosphatase activity. This information enabled us to assign Spo0F phosphatase activity to a Rap protein based on sequence alone, which was not previously possible. Finally, as the ultimate test of our newfound understanding of the structural requirements for Rap phosphatase function, a non-phosphatase Rap protein that inhibits the binding of the response regulator ComA to DNA was rationally engineered to dephosphorylate Spo0F. In addition to revealing the mechanistic basis of response regulator dephosphorylation by Rap proteins, our studies support the previously proposed T-loop-Y allostery model of receiver domain regulation that restricts the aromatic "switch" residue to an internal position when the beta4-alpha4 loop adopts an active-site proximal conformation.	+
-		+
-	~~Structural basis of response regulator dephosphorylation by Rap phosphatases.,Parashar V, Mirouze N, Dubnau DA, Neiditch MB PLoS Biol. 2011 Feb 8;9(2):e1000589. PMID:21346797<ref>PMID:21346797</ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3q15" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Phosphotransferase 3D structures\|Phosphotransferase 3D structures]]		*[[Phosphotransferase 3D structures\|Phosphotransferase 3D structures]]
	*[[Response regulator 3D structure\|Response regulator 3D structure]]		*[[Response regulator 3D structure\|Response regulator 3D structure]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Vibrio~~ subtilis ~~ehrenberg 1835~~]]	+	[[Category: Bacillus subtilis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Neiditch~~, M B~~]]	+	[[Category: Neiditch MB]]
-	[[Category: Parashar, V]]	+	[[Category: Parashar V]]
-	~~[[Category: 3-helix bundle]]~~	+
-	~~[[Category: Hydrolase-kinase complex]]~~	+
-	~~[[Category: Phosphatase]]~~	+
-	~~[[Category: Phosphorelay signal transduction]]~~	+
-	~~[[Category: Response regulator receiver]]~~	+
-	~~[[Category: Tetratricopeptide repeat~~]]	+

OCA at 09:05, 25 May 2022

OCA — Wed, 25 May 2022 09:05:37 GMT

←Older revision		Revision as of 09:05, 25 May 2022
Line 1:		Line 1:

	==Crystal Structure of RapH complexed with Spo0F==		==Crystal Structure of RapH complexed with Spo0F==
-	<StructureSection load='3q15' size='340' side='right' caption='[[3q15]], [[Resolution\|resolution]] 2.19Å' scene=''>	+	<StructureSection load='3q15' size='340' side='right'caption='[[3q15]], [[Resolution\|resolution]] 2.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3q15]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"~~bacillus_globigii~~"~~_migula_1900~~ "~~bacillus globigii~~" ~~migula 1900~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q15 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3Q15 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3q15]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q15 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3Q15 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU06830, rapH, yeeH, yzqA ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "~~Bacillus globigii~~" ~~Migula 1900~~]), BSU37130, spo0F ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "~~Bacillus globigii~~" ~~Migula 1900~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU06830, rapH, yeeH, yzqA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835]), BSU37130, spo0F ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3q15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q15 OCA], [~~http~~://pdbe.org/3q15 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3q15 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3q15 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3q15 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3q15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q15 OCA], [https://pdbe.org/3q15 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3q15 RCSB], [https://www.ebi.ac.uk/pdbsum/3q15 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3q15 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/SP0F_BACSU SP0F_BACSU]] Key element in the phosphorelay regulating sporulation initiation. Phosphorylation of spo0B during sporulation initiation.	+	[[https://www.uniprot.org/uniprot/SP0F_BACSU SP0F_BACSU]] Key element in the phosphorelay regulating sporulation initiation. Phosphorylation of spo0B during sporulation initiation.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:

	==See Also==		==See Also==
-	*[[Phosphotransferase\|Phosphotransferase]]	+	*[[Phosphotransferase 3D structures\|Phosphotransferase 3D structures]]
-	*[[Response regulator\|Response regulator]]	+	*[[Response regulator 3D structure\|Response regulator 3D structure]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus globigii migula 1900~~]]	+	[[Category: Vibrio subtilis ehrenberg 1835]]
		+	[[Category: Large Structures]]
	[[Category: Neiditch, M B]]		[[Category: Neiditch, M B]]
	[[Category: Parashar, V]]		[[Category: Parashar, V]]

OCA at 20:04, 5 August 2016

OCA — Fri, 05 Aug 2016 20:04:58 GMT

←Older revision		Revision as of 20:04, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of RapH complexed with Spo0F==		==Crystal Structure of RapH complexed with Spo0F==
	<StructureSection load='3q15' size='340' side='right' caption='[[3q15]], [[Resolution\|resolution]] 2.19Å' scene=''>		<StructureSection load='3q15' size='340' side='right' caption='[[3q15]], [[Resolution\|resolution]] 2.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3q15]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_subtilis Bacillus subtilis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q15 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3Q15 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3q15]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q15 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3Q15 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU06830, rapH, yeeH, yzqA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus ~~subtilis~~]), BSU37130, spo0F ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus ~~subtilis~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU06830, rapH, yeeH, yzqA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Bacillus globigii" Migula 1900]), BSU37130, spo0F ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Bacillus globigii" Migula 1900])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3q15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q15 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3q15 RCSB], [http://www.ebi.ac.uk/pdbsum/3q15 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3q15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q15 OCA], [http://pdbe.org/3q15 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3q15 RCSB], [http://www.ebi.ac.uk/pdbsum/3q15 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3q15 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3q15" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus ~~subtilis~~]]	+	[[Category: Bacillus globigii migula 1900]]
	[[Category: Neiditch, M B]]		[[Category: Neiditch, M B]]
	[[Category: Parashar, V]]		[[Category: Parashar, V]]

OCA at 21:31, 25 December 2014

OCA — Thu, 25 Dec 2014 21:31:24 GMT

←Older revision		Revision as of 21:31, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3q15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q15 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3q15 RCSB], [http://www.ebi.ac.uk/pdbsum/3q15 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3q15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q15 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3q15 RCSB], [http://www.ebi.ac.uk/pdbsum/3q15 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SP0F_BACSU SP0F_BACSU]] Key element in the phosphorelay regulating sporulation initiation. Phosphorylation of spo0B during sporulation initiation.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 12:56, 9 December 2014

OCA — Tue, 09 Dec 2014 12:56:37 GMT

←Older revision		Revision as of 12:56, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3q15.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal Structure of RapH complexed with Spo0F==
		+	<StructureSection load='3q15' size='340' side='right' caption='[[3q15]], [[Resolution\|resolution]] 2.19Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3q15]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q15 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3Q15 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BSU06830, rapH, yeeH, yzqA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis]), BSU37130, spo0F ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3q15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q15 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3q15 RCSB], [http://www.ebi.ac.uk/pdbsum/3q15 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacterial Rap family proteins have been most extensively studied in Bacillus subtilis, where they regulate activities including sporulation, genetic competence, antibiotic expression, and the movement of the ICEBs1 transposon. One subset of Rap proteins consists of phosphatases that control B. subtilis and B. anthracis sporulation by dephosphorylating the response regulator Spo0F. The mechanistic basis of Rap phosphatase activity was unknown. Here we present the RapH-Spo0F X-ray crystal structure, which shows that Rap proteins consist of a 3-helix bundle and a tetratricopeptide repeat domain. Extensive biochemical and genetic functional studies reveal the importance of the observed RapH-Spo0F interactions, including the catalytic role of a glutamine in the RapH 3-helix bundle that inserts into the Spo0F active site. We show that in addition to dephosphorylating Spo0F, RapH can antagonize sporulation by sterically blocking phosphoryl transfer to and from Spo0F. Our structure-function analysis of the RapH-Spo0F interaction identified Rap protein residues critical for Spo0F phosphatase activity. This information enabled us to assign Spo0F phosphatase activity to a Rap protein based on sequence alone, which was not previously possible. Finally, as the ultimate test of our newfound understanding of the structural requirements for Rap phosphatase function, a non-phosphatase Rap protein that inhibits the binding of the response regulator ComA to DNA was rationally engineered to dephosphorylate Spo0F. In addition to revealing the mechanistic basis of response regulator dephosphorylation by Rap proteins, our studies support the previously proposed T-loop-Y allostery model of receiver domain regulation that restricts the aromatic "switch" residue to an internal position when the beta4-alpha4 loop adopts an active-site proximal conformation.

-	~~{{STRUCTURE_3q15\| PDB=3q15 \| SCENE= }}~~	+	Structural basis of response regulator dephosphorylation by Rap phosphatases.,Parashar V, Mirouze N, Dubnau DA, Neiditch MB PLoS Biol. 2011 Feb 8;9(2):e1000589. PMID:21346797<ref>PMID:21346797</ref>

-	~~===Crystal Structure~~ of ~~RapH complexed with Spo0F===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_21346797}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3q15]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bacillus_subtilis Bacillus subtilis]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3Q15 OCA].~~	+

	==See Also==		==See Also==
	*[[Phosphotransferase\|Phosphotransferase]]		*[[Phosphotransferase\|Phosphotransferase]]
	*[[Response regulator\|Response regulator]]		*[[Response regulator\|Response regulator]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:021346797<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bacillus subtilis]]		[[Category: Bacillus subtilis]]
-	[[Category: Neiditch, M B.]]	+	[[Category: Neiditch, M B]]
-	[[Category: Parashar, V.]]	+	[[Category: Parashar, V]]
	[[Category: 3-helix bundle]]		[[Category: 3-helix bundle]]
	[[Category: Hydrolase-kinase complex]]		[[Category: Hydrolase-kinase complex]]

OCA at 21:54, 20 October 2012

OCA — Sat, 20 Oct 2012 21:54:03 GMT

←Older revision		Revision as of 21:54, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3q15]] is a 4 chain structure ~~of [[Phosphotransferase]] and [[Response regulator]]~~ with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q15 OCA].	+	[[3q15]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q15 OCA].

	==See Also==		==See Also==

OCA at 14:36, 27 July 2012

OCA — Fri, 27 Jul 2012 14:36:08 GMT

←Older revision		Revision as of 14:36, 27 July 2012
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	[[Image:3q15.png\|left\|200px]]		[[Image:3q15.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3q15", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3q15\| PDB=3q15 \| SCENE= }}		{{STRUCTURE_3q15\| PDB=3q15 \| SCENE= }}

	===Crystal Structure of RapH complexed with Spo0F===		===Crystal Structure of RapH complexed with Spo0F===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21346797}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 21346797 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_21346797}}		{{ABSTRACT_PUBMED_21346797}}

	==About this Structure==		==About this Structure==
-	[[3q15]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q15 OCA].	+	[[3q15]] is a 4 chain structure of [[Phosphotransferase]] and [[Response regulator]] with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q15 OCA].
		+
		+	==See Also==
		+	*[[Phosphotransferase\|Phosphotransferase]]
		+	*[[Response regulator\|Response regulator]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~21346797~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:021346797</ref><references group="xtra"/>
	[[Category: Bacillus subtilis]]		[[Category: Bacillus subtilis]]
	[[Category: Neiditch, M B.]]		[[Category: Neiditch, M B.]]
	[[Category: Parashar, V.]]		[[Category: Parashar, V.]]
		+	[[Category: 3-helix bundle]]
		+	[[Category: Hydrolase-kinase complex]]
		+	[[Category: Phosphatase]]
		+	[[Category: Phosphorelay signal transduction]]
		+	[[Category: Response regulator receiver]]
		+	[[Category: Tetratricopeptide repeat]]

OCA at 09:55, 21 April 2011

OCA — Thu, 21 Apr 2011 09:55:41 GMT

←Older revision		Revision as of 09:55, 21 April 2011
Line 1:		Line 1:
-	[[Image:3q15.~~jpg~~\|left\|200px]]	+	[[Image:3q15.png\|left\|200px]]

	<!--		<!--
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	===Crystal Structure of RapH complexed with Spo0F===		===Crystal Structure of RapH complexed with Spo0F===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21346797}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21346797 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21346797}}

	==About this Structure==		==About this Structure==
	[[3q15]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q15 OCA].		[[3q15]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q15 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:21346797</ref><references group="xtra"/>
	[[Category: Bacillus subtilis]]		[[Category: Bacillus subtilis]]
	[[Category: Neiditch, M B.]]		[[Category: Neiditch, M B.]]
	[[Category: Parashar, V.]]		[[Category: Parashar, V.]]

OCA at 09:59, 23 February 2011

OCA — Wed, 23 Feb 2011 09:59:36 GMT

←Older revision		Revision as of 09:59, 23 February 2011
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-	~~'''Unreleased structure'''~~	+	[[Image:3q15.jpg\|left\|200px]]

-	The ~~entry 3q15~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3q15", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3q15\| PDB=3q15 \| SCENE= }}

-	~~Authors: Parashar, V., Neiditch, M.B.~~	+	===Crystal Structure of RapH complexed with Spo0F===

-	~~Description: Crystal~~ Structure ~~of RapH complexed~~ with ~~Spo0F~~	+
		+	==About this Structure==
		+	[[3q15]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q15 OCA].
		+	[[Category: Bacillus subtilis]]
		+	[[Category: Neiditch, M B.]]
		+	[[Category: Parashar, V.]]

OCA: Protected "3q15" [edit=sysop:move=sysop]

OCA — Wed, 19 Jan 2011 05:49:58 GMT

Protected "3q15" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:49, 19 January 2011