3q2t - Revision history

OCA at 11:31, 14 March 2024

OCA — Thu, 14 Mar 2024 11:31:29 GMT

←Older revision		Revision as of 11:31, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3q2t' size='340' side='right'caption='[[3q2t]], [[Resolution\|resolution]] 3.06Å' scene=''>		<StructureSection load='3q2t' size='340' side='right'caption='[[3q2t]], [[Resolution\|resolution]] 3.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3q2t]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q2T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3Q2T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3q2t]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q2T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3Q2T FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[3q2s\|3q2s]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.061Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">CFIM25, CPSF25, CPSF5, NUDT21 ([https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=9606 HUMAN]), CFIM68, CPSF6 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3q2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q2t OCA], [https://pdbe.org/3q2t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3q2t RCSB], [https://www.ebi.ac.uk/pdbsum/3q2t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3q2t ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3q2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q2t OCA], [https://pdbe.org/3q2t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3q2t RCSB], [https://www.ebi.ac.uk/pdbsum/3q2t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3q2t ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CPSF5_HUMAN CPSF5_HUMAN]] Component of the cleavage factor Im (CFIm) complex that plays a key role in pre-mRNA 3'-processing. Involved in association with CPSF6 or CPSF7 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. NUDT21/CPSF5 binds to cleavage and polyadenylation RNA substrates. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides. May have a role in mRNA export.<ref>PMID:9659921</ref> <ref>PMID:8626397</ref> <ref>PMID:14690600</ref> <ref>PMID:20695905</ref> <ref>PMID:20479262</ref> <ref>PMID:21295486</ref> [[https://www.uniprot.org/uniprot/CPSF6_HUMAN CPSF6_HUMAN]] Component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3'-processing. Involved in association with NUDT21/CPSF5 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. CPSF6 binds to cleavage and polyadenylation RNA substrates and promotes RNA looping.<ref>PMID:9659921</ref> <ref>PMID:8626397</ref> <ref>PMID:14690600</ref> <ref>PMID:20695905</ref> <ref>PMID:21295486</ref>	+	[https://www.uniprot.org/uniprot/CPSF6_HUMAN CPSF6_HUMAN] Component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3'-processing. Involved in association with NUDT21/CPSF5 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. CPSF6 binds to cleavage and polyadenylation RNA substrates and promotes RNA looping.<ref>PMID:9659921</ref> <ref>PMID:8626397</ref> <ref>PMID:14690600</ref> <ref>PMID:20695905</ref> <ref>PMID:21295486</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Cleavage factor I(m) (CFI(m)) is a highly conserved component of the eukaryotic mRNA 3' processing machinery that functions in sequence-specific poly(A) site recognition through the collaboration of a 25 kDa subunit containing a Nudix domain and a larger subunit of 59, 68, or 72 kDa containing an RNA recognition motif (RRM). Our previous work demonstrated that CFI(m)25 is both necessary and sufficient for sequence-specific binding of the poly(A) site upstream element UGUA. Here, we report the crystal structure of CFI(m)25 complexed with the RRM domain of CFI(m)68 and RNA. The CFI(m)25 dimer is clasped on opposite sides by two CFI(m)68 RRM domains. Each CFI(m)25 subunit binds one UGUA element specifically. Biochemical analysis indicates that the CFI(m)68 RRMs serve to enhance RNA binding and facilitate RNA looping. The intrinsic ability of CFI(m) to direct RNA looping may provide a mechanism for its function in the regulation of alternative poly(A) site selection.	+
-		+
-	Crystal Structure of a Human Cleavage Factor CFI(m)25/CFI(m)68/RNA Complex Provides an Insight into Poly(A) Site Recognition and RNA Looping.,Yang Q, Coseno M, Gilmartin GM, Doublie S Structure. 2011 Feb 2. PMID:21295486<ref>PMID:21295486</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3q2t" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Coseno, M]]	+	[[Category: Coseno M]]
-	[[Category: Doublie, S]]	+	[[Category: Doublie S]]
-	[[Category: Gilmartin~~, G M~~]]	+	[[Category: Gilmartin GM]]
-	[[Category: Yang, Q]]	+	[[Category: Yang Q]]
-	~~[[Category: 3' end processing]]~~	+
-	~~[[Category: Cfim]]~~	+
-	~~[[Category: Cfim25]]~~	+
-	~~[[Category: Cfim68]]~~	+
-	~~[[Category: Cleavage factor]]~~	+
-	~~[[Category: Cpsf]]~~	+
-	~~[[Category: Cpsf5]]~~	+
-	~~[[Category: Cpsf6]]~~	+
-	~~[[Category: Nudix fold]]~~	+
-	~~[[Category: Nudix protein]]~~	+
-	~~[[Category: Protein-protein complex]]~~	+
-	~~[[Category: Protein-rna complex]]~~	+
-	~~[[Category: Rna binding protein-rna complex]]~~	+
-	~~[[Category: Rna processing]]~~	+
-	~~[[Category: Rrm~~]]	+

OCA at 05:49, 8 June 2022

OCA — Wed, 08 Jun 2022 05:49:28 GMT

←Older revision		Revision as of 05:49, 8 June 2022
Line 1:		Line 1:

	==Crystal Structure of CFIm68 RRM/CFIm25/RNA complex==		==Crystal Structure of CFIm68 RRM/CFIm25/RNA complex==
-	<StructureSection load='3q2t' size='340' side='right' caption='[[3q2t]], [[Resolution\|resolution]] 3.06Å' scene=''>	+	<StructureSection load='3q2t' size='340' side='right'caption='[[3q2t]], [[Resolution\|resolution]] 3.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3q2t]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q2T OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3Q2T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3q2t]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q2T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3Q2T FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3q2s\|3q2s]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3q2s\|3q2s]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CFIM25, CPSF25, CPSF5, NUDT21 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CFIM68, CPSF6 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CFIM25, CPSF25, CPSF5, NUDT21 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CFIM68, CPSF6 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3q2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q2t OCA], [~~http~~://pdbe.org/3q2t PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3q2t RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3q2t PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3q2t ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3q2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q2t OCA], [https://pdbe.org/3q2t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3q2t RCSB], [https://www.ebi.ac.uk/pdbsum/3q2t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3q2t ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CPSF5_HUMAN CPSF5_HUMAN]] Component of the cleavage factor Im (CFIm) complex that plays a key role in pre-mRNA 3'-processing. Involved in association with CPSF6 or CPSF7 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. NUDT21/CPSF5 binds to cleavage and polyadenylation RNA substrates. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides. May have a role in mRNA export.<ref>PMID:9659921</ref> <ref>PMID:8626397</ref> <ref>PMID:14690600</ref> <ref>PMID:20695905</ref> <ref>PMID:20479262</ref> <ref>PMID:21295486</ref> [[~~http~~://www.uniprot.org/uniprot/CPSF6_HUMAN CPSF6_HUMAN]] Component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3'-processing. Involved in association with NUDT21/CPSF5 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. CPSF6 binds to cleavage and polyadenylation RNA substrates and promotes RNA looping.<ref>PMID:9659921</ref> <ref>PMID:8626397</ref> <ref>PMID:14690600</ref> <ref>PMID:20695905</ref> <ref>PMID:21295486</ref>	+	[[https://www.uniprot.org/uniprot/CPSF5_HUMAN CPSF5_HUMAN]] Component of the cleavage factor Im (CFIm) complex that plays a key role in pre-mRNA 3'-processing. Involved in association with CPSF6 or CPSF7 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. NUDT21/CPSF5 binds to cleavage and polyadenylation RNA substrates. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides. May have a role in mRNA export.<ref>PMID:9659921</ref> <ref>PMID:8626397</ref> <ref>PMID:14690600</ref> <ref>PMID:20695905</ref> <ref>PMID:20479262</ref> <ref>PMID:21295486</ref> [[https://www.uniprot.org/uniprot/CPSF6_HUMAN CPSF6_HUMAN]] Component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3'-processing. Involved in association with NUDT21/CPSF5 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. CPSF6 binds to cleavage and polyadenylation RNA substrates and promotes RNA looping.<ref>PMID:9659921</ref> <ref>PMID:8626397</ref> <ref>PMID:14690600</ref> <ref>PMID:20695905</ref> <ref>PMID:21295486</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Coseno, M]]		[[Category: Coseno, M]]
	[[Category: Doublie, S]]		[[Category: Doublie, S]]

OCA at 12:44, 5 August 2016

OCA — Fri, 05 Aug 2016 12:44:09 GMT

←Older revision		Revision as of 12:44, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of CFIm68 RRM/CFIm25/RNA complex==		==Crystal Structure of CFIm68 RRM/CFIm25/RNA complex==
	<StructureSection load='3q2t' size='340' side='right' caption='[[3q2t]], [[Resolution\|resolution]] 3.06Å' scene=''>		<StructureSection load='3q2t' size='340' side='right' caption='[[3q2t]], [[Resolution\|resolution]] 3.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3q2t]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q2T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3Q2T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3q2t]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q2T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3Q2T FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3q2s\|3q2s]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3q2s\|3q2s]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CFIM25, CPSF25, CPSF5, NUDT21 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~]), CFIM68, CPSF6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CFIM25, CPSF25, CPSF5, NUDT21 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CFIM68, CPSF6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3q2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q2t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3q2t RCSB], [http://www.ebi.ac.uk/pdbsum/3q2t PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3q2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q2t OCA], [http://pdbe.org/3q2t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3q2t RCSB], [http://www.ebi.ac.uk/pdbsum/3q2t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3q2t ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3q2t" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Coseno, M]]		[[Category: Coseno, M]]
	[[Category: Doublie, S]]		[[Category: Doublie, S]]

OCA at 18:56, 25 December 2014

OCA — Thu, 25 Dec 2014 18:56:47 GMT

←Older revision		Revision as of 18:56, 25 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3q2t\| PDB=3q2t \| SCENE= }}~~	+	==Crystal Structure of CFIm68 RRM/CFIm25/RNA complex==
-	===Crystal Structure of CFIm68 RRM/CFIm25/RNA complex===	+	<StructureSection load='3q2t' size='340' side='right' caption='[[3q2t]], [[Resolution\|resolution]] 3.06Å' scene=''>
-	~~{{ABSTRACT_PUBMED_21295486}}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[3q2t]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q2T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3Q2T FirstGlance]. <br>
-	==Function==	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3q2s\|3q2s]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CFIM25, CPSF25, CPSF5, NUDT21 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), CFIM68, CPSF6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3q2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q2t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3q2t RCSB], [http://www.ebi.ac.uk/pdbsum/3q2t PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
	[[http://www.uniprot.org/uniprot/CPSF5_HUMAN CPSF5_HUMAN]] Component of the cleavage factor Im (CFIm) complex that plays a key role in pre-mRNA 3'-processing. Involved in association with CPSF6 or CPSF7 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. NUDT21/CPSF5 binds to cleavage and polyadenylation RNA substrates. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides. May have a role in mRNA export.<ref>PMID:9659921</ref> <ref>PMID:8626397</ref> <ref>PMID:14690600</ref> <ref>PMID:20695905</ref> <ref>PMID:20479262</ref> <ref>PMID:21295486</ref> [[http://www.uniprot.org/uniprot/CPSF6_HUMAN CPSF6_HUMAN]] Component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3'-processing. Involved in association with NUDT21/CPSF5 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. CPSF6 binds to cleavage and polyadenylation RNA substrates and promotes RNA looping.<ref>PMID:9659921</ref> <ref>PMID:8626397</ref> <ref>PMID:14690600</ref> <ref>PMID:20695905</ref> <ref>PMID:21295486</ref>		[[http://www.uniprot.org/uniprot/CPSF5_HUMAN CPSF5_HUMAN]] Component of the cleavage factor Im (CFIm) complex that plays a key role in pre-mRNA 3'-processing. Involved in association with CPSF6 or CPSF7 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. NUDT21/CPSF5 binds to cleavage and polyadenylation RNA substrates. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides. May have a role in mRNA export.<ref>PMID:9659921</ref> <ref>PMID:8626397</ref> <ref>PMID:14690600</ref> <ref>PMID:20695905</ref> <ref>PMID:20479262</ref> <ref>PMID:21295486</ref> [[http://www.uniprot.org/uniprot/CPSF6_HUMAN CPSF6_HUMAN]] Component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3'-processing. Involved in association with NUDT21/CPSF5 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. CPSF6 binds to cleavage and polyadenylation RNA substrates and promotes RNA looping.<ref>PMID:9659921</ref> <ref>PMID:8626397</ref> <ref>PMID:14690600</ref> <ref>PMID:20695905</ref> <ref>PMID:21295486</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cleavage factor I(m) (CFI(m)) is a highly conserved component of the eukaryotic mRNA 3' processing machinery that functions in sequence-specific poly(A) site recognition through the collaboration of a 25 kDa subunit containing a Nudix domain and a larger subunit of 59, 68, or 72 kDa containing an RNA recognition motif (RRM). Our previous work demonstrated that CFI(m)25 is both necessary and sufficient for sequence-specific binding of the poly(A) site upstream element UGUA. Here, we report the crystal structure of CFI(m)25 complexed with the RRM domain of CFI(m)68 and RNA. The CFI(m)25 dimer is clasped on opposite sides by two CFI(m)68 RRM domains. Each CFI(m)25 subunit binds one UGUA element specifically. Biochemical analysis indicates that the CFI(m)68 RRMs serve to enhance RNA binding and facilitate RNA looping. The intrinsic ability of CFI(m) to direct RNA looping may provide a mechanism for its function in the regulation of alternative poly(A) site selection.

-	~~==About this~~ Structure==	+	Crystal Structure of a Human Cleavage Factor CFI(m)25/CFI(m)68/RNA Complex Provides an Insight into Poly(A) Site Recognition and RNA Looping.,Yang Q, Coseno M, Gilmartin GM, Doublie S Structure. 2011 Feb 2. PMID:21295486<ref>PMID:21295486</ref>
-	~~[[3q2t]] is~~ a ~~6 chain structure with sequence from [http:~~//en.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http~~:/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3Q2T OCA].~~	+

-	==~~Reference~~==	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~<ref group="xtra">PMID:021295486</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Coseno, M.]]	+	[[Category: Coseno, M]]
-	[[Category: Doublie, S.]]	+	[[Category: Doublie, S]]
-	[[Category: Gilmartin, G M.]]	+	[[Category: Gilmartin, G M]]
-	[[Category: Yang, Q.]]	+	[[Category: Yang, Q]]
	[[Category: 3' end processing]]		[[Category: 3' end processing]]
	[[Category: Cfim]]		[[Category: Cfim]]

OCA at 08:26, 8 May 2013

OCA — Wed, 08 May 2013 08:26:50 GMT

←Older revision		Revision as of 08:26, 8 May 2013
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-	[[Image:3q2t.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3q2t", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3q2t\| PDB=3q2t \| SCENE= }}		{{STRUCTURE_3q2t\| PDB=3q2t \| SCENE= }}
-
	===Crystal Structure of CFIm68 RRM/CFIm25/RNA complex===		===Crystal Structure of CFIm68 RRM/CFIm25/RNA complex===
		+	{{ABSTRACT_PUBMED_21295486}}

-		+	==Function==
-	<!--	+	[[http://www.uniprot.org/uniprot/CPSF5_HUMAN CPSF5_HUMAN]] Component of the cleavage factor Im (CFIm) complex that plays a key role in pre-mRNA 3'-processing. Involved in association with CPSF6 or CPSF7 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. NUDT21/CPSF5 binds to cleavage and polyadenylation RNA substrates. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides. May have a role in mRNA export.<ref>PMID:9659921</ref> <ref>PMID:8626397</ref> <ref>PMID:14690600</ref> <ref>PMID:20695905</ref> <ref>PMID:20479262</ref> <ref>PMID:21295486</ref> [[http://www.uniprot.org/uniprot/CPSF6_HUMAN CPSF6_HUMAN]] Component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3'-processing. Involved in association with NUDT21/CPSF5 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. CPSF6 binds to cleavage and polyadenylation RNA substrates and promotes RNA looping.<ref>PMID:9659921</ref> <ref>PMID:8626397</ref> <ref>PMID:14690600</ref> <ref>PMID:20695905</ref> <ref>PMID:21295486</ref>
-	The ~~line below this paragraph, {{ABSTRACT_PUBMED_21295486}}, adds~~ the ~~Publication Abstract~~ to ~~the page~~	+
-	~~(as it appears on PubMed at~~ http://www.~~pubmed~~.~~gov), where 21295486 is~~ the ~~PubMed ID number~~.	+
-	-->	+
-	~~{{ABSTRACT_PUBMED_21295486}}~~	+

	==About this Structure==		==About this Structure==
Line 22:		Line 10:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~21295486~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:021295486</ref><references group="xtra"/><references/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Coseno, M.]]		[[Category: Coseno, M.]]
Line 28:		Line 16:
	[[Category: Gilmartin, G M.]]		[[Category: Gilmartin, G M.]]
	[[Category: Yang, Q.]]		[[Category: Yang, Q.]]
		+	[[Category: 3' end processing]]
		+	[[Category: Cfim]]
		+	[[Category: Cfim25]]
		+	[[Category: Cfim68]]
		+	[[Category: Cleavage factor]]
		+	[[Category: Cpsf]]
		+	[[Category: Cpsf5]]
		+	[[Category: Cpsf6]]
		+	[[Category: Nudix fold]]
		+	[[Category: Nudix protein]]
		+	[[Category: Protein-protein complex]]
		+	[[Category: Protein-rna complex]]
		+	[[Category: Rna binding protein-rna complex]]
		+	[[Category: Rna processing]]
		+	[[Category: Rrm]]

OCA at 09:25, 23 February 2011

OCA — Wed, 23 Feb 2011 09:25:13 GMT

←Older revision		Revision as of 09:25, 23 February 2011
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-	[[Image:3q2t.~~jpg~~\|left\|200px]]	+	[[Image:3q2t.png\|left\|200px]]

	<!--		<!--
Line 24:		Line 24:
	<ref group="xtra">PMID:21295486</ref><references group="xtra"/>		<ref group="xtra">PMID:21295486</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
		+	[[Category: Coseno, M.]]
		+	[[Category: Doublie, S.]]
		+	[[Category: Gilmartin, G M.]]
	[[Category: Yang, Q.]]		[[Category: Yang, Q.]]

OCA at 05:42, 16 February 2011

OCA — Wed, 16 Feb 2011 05:42:12 GMT

←Older revision		Revision as of 05:42, 16 February 2011
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-	~~'''Unreleased structure'''~~	+	[[Image:3q2t.jpg\|left\|200px]]

-	The ~~entry 3q2t~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3q2t", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3q2t\| PDB=3q2t \| SCENE= }}

-	~~Authors: Yang, Q.~~	+	===Crystal Structure of CFIm68 RRM/CFIm25/RNA complex===

-	~~Description~~: ~~Crystal Stucture of nudt21~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21295486}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21295486 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21295486}}
		+
		+	==About this Structure==
		+	[[3q2t]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q2T OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:21295486</ref><references group="xtra"/>
		+	[[Category: Homo sapiens]]
		+	[[Category: Yang, Q.]]

OCA: Protected "3q2t" [edit=sysop:move=sysop]

OCA — Wed, 29 Dec 2010 18:17:42 GMT

Protected "3q2t" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:17, 29 December 2010

OCA: New page: '''Unreleased structure''' The entry 3q2t is ON HOLD Authors: Yang, Q. Description: Crystal Stucture of nudt21

OCA — Wed, 29 Dec 2010 18:17:41 GMT

New page: '''Unreleased structure''' The entry 3q2t is ON HOLD Authors: Yang, Q. Description: Crystal Stucture of nudt21

New page

'''Unreleased structure'''

The entry 3q2t is ON HOLD

Authors: Yang, Q.

Description: Crystal Stucture of nudt21