3q7p - Revision history

OCA at 11:36, 14 March 2024

OCA — Thu, 14 Mar 2024 11:36:02 GMT

←Older revision		Revision as of 11:36, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3q7p' size='340' side='right'caption='[[3q7p]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3q7p' size='340' side='right'caption='[[3q7p]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3q7p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q7P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3Q7P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3q7p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q7P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3Q7P FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID~~-~~GUANYLATE+ESTER'>GNP</scene>~~, ~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[2dpx\|2dpx]], [[3q72\|3q72]], [[3q7q\|3q7q]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">RRAD, RAD ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3q7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q7p OCA], [https://pdbe.org/3q7p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3q7p RCSB], [https://www.ebi.ac.uk/pdbsum/3q7p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3q7p ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3q7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q7p OCA], [https://pdbe.org/3q7p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3q7p RCSB], [https://www.ebi.ac.uk/pdbsum/3q7p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3q7p ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/RAD_HUMAN RAD_HUMAN]] May play an important role in cardiac antiarrhythmia via the strong suppression of voltage-gated L-type Ca(2+) currents. Regulates voltage-dependent L-type calcium channel subunit alpha-1C trafficking to the cell membrane (By similarity). Inhibits cardiac hypertrophy through the calmodulin-dependent kinase II (CaMKII) pathway. Inhibits phosphorylation and activation of CAMK2D.<ref>PMID:18056528</ref>	+	[https://www.uniprot.org/uniprot/RAD_HUMAN RAD_HUMAN] May play an important role in cardiac antiarrhythmia via the strong suppression of voltage-gated L-type Ca(2+) currents. Regulates voltage-dependent L-type calcium channel subunit alpha-1C trafficking to the cell membrane (By similarity). Inhibits cardiac hypertrophy through the calmodulin-dependent kinase II (CaMKII) pathway. Inhibits phosphorylation and activation of CAMK2D.<ref>PMID:18056528</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The RGK family of small G-proteins, including Rad, Gem, Rem1, and Rem2, is inducibly expressed in various mammalian tissues and interacts with voltage-dependent calcium channels and Rho kinase. Many questions remain regarding their physiological roles and molecular mechanism. Previous crystallographic studies reported RGK G-domain:guanosine di-phosphate structures. To test whether RGK proteins undergo a nucleotide-induced conformational change, we determined the crystallographic structures of Rad:GppNHp and Rem2:GppNHp to 1.7 and 1.8 A resolutions, respectively. Also, we characterized the nucleotide-binding properties and conformations for Gem, Rad, and several structure-based mutants using fluorescence spectroscopy. The results suggest that RGK G-proteins may not behave as Ras-like canonical nucleotide-induced molecular switches. Further, the RGK proteins have differing structures and nucleotide-binding properties, which may have implications for their varied action on effectors.	+
-		+
-	~~RGK Family G-Domain:GTP Analog Complex Structures and Nucleotide-Binding Properties.,Sasson Y, Navon-Perry L, Huppert D, Hirsch JA J Mol Biol. 2011 Aug 29. PMID:21903096<ref>PMID:21903096</ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3q7p" style="background-color:#fffaf0;"></div~~>	+

	==See Also==		==See Also==
Line 27:		Line 17:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hirsch~~, J A~~]]	+	[[Category: Hirsch JA]]
-	[[Category: Navon-Perry, L]]	+	[[Category: Navon-Perry L]]
-	[[Category: Sasson, Y]]	+	[[Category: Sasson Y]]
-	~~[[Category: Cav2 beta]]~~	+
-	~~[[Category: G-domain]]~~	+
-	~~[[Category: G-protein]]~~	+
-	~~[[Category: Signaling protein~~]]	+

OCA at 05:56, 8 June 2022

OCA — Wed, 08 Jun 2022 05:56:27 GMT

←Older revision		Revision as of 05:56, 8 June 2022
Line 1:		Line 1:

	==Crystal Structure of Rad G-domain-GTP Analog Complex==		==Crystal Structure of Rad G-domain-GTP Analog Complex==
-	<StructureSection load='3q7p' size='340' side='right' caption='[[3q7p]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='3q7p' size='340' side='right'caption='[[3q7p]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3q7p]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q7P OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3Q7P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3q7p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q7P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3Q7P FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2dpx\|2dpx]], [[3q72\|3q72]], [[3q7q\|3q7q]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2dpx\|2dpx]], [[3q72\|3q72]], [[3q7q\|3q7q]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RRAD, RAD ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RRAD, RAD ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3q7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q7p OCA], [~~http~~://pdbe.org/3q7p PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3q7p RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3q7p PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3q7p ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3q7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q7p OCA], [https://pdbe.org/3q7p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3q7p RCSB], [https://www.ebi.ac.uk/pdbsum/3q7p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3q7p ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/RAD_HUMAN RAD_HUMAN]] May play an important role in cardiac antiarrhythmia via the strong suppression of voltage-gated L-type Ca(2+) currents. Regulates voltage-dependent L-type calcium channel subunit alpha-1C trafficking to the cell membrane (By similarity). Inhibits cardiac hypertrophy through the calmodulin-dependent kinase II (CaMKII) pathway. Inhibits phosphorylation and activation of CAMK2D.<ref>PMID:18056528</ref>	+	[[https://www.uniprot.org/uniprot/RAD_HUMAN RAD_HUMAN]] May play an important role in cardiac antiarrhythmia via the strong suppression of voltage-gated L-type Ca(2+) currents. Regulates voltage-dependent L-type calcium channel subunit alpha-1C trafficking to the cell membrane (By similarity). Inhibits cardiac hypertrophy through the calmodulin-dependent kinase II (CaMKII) pathway. Inhibits phosphorylation and activation of CAMK2D.<ref>PMID:18056528</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:

	==See Also==		==See Also==
-	*[[GTP-binding protein\|GTP-binding protein]]	+	*[[GTP-binding protein 3D structures\|GTP-binding protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 28:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Hirsch, J A]]		[[Category: Hirsch, J A]]
	[[Category: Navon-Perry, L]]		[[Category: Navon-Perry, L]]

OCA at 10:58, 4 August 2016

OCA — Thu, 04 Aug 2016 10:58:16 GMT

←Older revision		Revision as of 10:58, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of Rad G-domain-GTP Analog Complex==		==Crystal Structure of Rad G-domain-GTP Analog Complex==
	<StructureSection load='3q7p' size='340' side='right' caption='[[3q7p]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3q7p' size='340' side='right' caption='[[3q7p]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3q7p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q7P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3Q7P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3q7p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q7P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3Q7P FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2dpx\|2dpx]], [[3q72\|3q72]], [[3q7q\|3q7q]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2dpx\|2dpx]], [[3q72\|3q72]], [[3q7q\|3q7q]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RRAD, RAD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RRAD, RAD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3q7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q7p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3q7p RCSB], [http://www.ebi.ac.uk/pdbsum/3q7p PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3q7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q7p OCA], [http://pdbe.org/3q7p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3q7p RCSB], [http://www.ebi.ac.uk/pdbsum/3q7p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3q7p ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3q7p" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Hirsch, J A]]		[[Category: Hirsch, J A]]
	[[Category: Navon-Perry, L]]		[[Category: Navon-Perry, L]]

OCA at 08:36, 25 December 2014

OCA — Thu, 25 Dec 2014 08:36:00 GMT

←Older revision		Revision as of 08:36, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3q7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q7p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3q7p RCSB], [http://www.ebi.ac.uk/pdbsum/3q7p PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3q7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q7p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3q7p RCSB], [http://www.ebi.ac.uk/pdbsum/3q7p PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RAD_HUMAN RAD_HUMAN]] May play an important role in cardiac antiarrhythmia via the strong suppression of voltage-gated L-type Ca(2+) currents. Regulates voltage-dependent L-type calcium channel subunit alpha-1C trafficking to the cell membrane (By similarity). Inhibits cardiac hypertrophy through the calmodulin-dependent kinase II (CaMKII) pathway. Inhibits phosphorylation and activation of CAMK2D.<ref>PMID:18056528</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 12:25, 9 December 2014

OCA — Tue, 09 Dec 2014 12:25:17 GMT

←Older revision		Revision as of 12:25, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3q7p.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal Structure of Rad G-domain-GTP Analog Complex==
		+	<StructureSection load='3q7p' size='340' side='right' caption='[[3q7p]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3q7p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q7P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3Q7P FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[2dpx\|2dpx]], [[3q72\|3q72]], [[3q7q\|3q7q]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RRAD, RAD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3q7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q7p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3q7p RCSB], [http://www.ebi.ac.uk/pdbsum/3q7p PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The RGK family of small G-proteins, including Rad, Gem, Rem1, and Rem2, is inducibly expressed in various mammalian tissues and interacts with voltage-dependent calcium channels and Rho kinase. Many questions remain regarding their physiological roles and molecular mechanism. Previous crystallographic studies reported RGK G-domain:guanosine di-phosphate structures. To test whether RGK proteins undergo a nucleotide-induced conformational change, we determined the crystallographic structures of Rad:GppNHp and Rem2:GppNHp to 1.7 and 1.8 A resolutions, respectively. Also, we characterized the nucleotide-binding properties and conformations for Gem, Rad, and several structure-based mutants using fluorescence spectroscopy. The results suggest that RGK G-proteins may not behave as Ras-like canonical nucleotide-induced molecular switches. Further, the RGK proteins have differing structures and nucleotide-binding properties, which may have implications for their varied action on effectors.

-	~~{{STRUCTURE_3q7p\| PDB=3q7p \| SCENE= }}~~	+	RGK Family G-Domain:GTP Analog Complex Structures and Nucleotide-Binding Properties.,Sasson Y, Navon-Perry L, Huppert D, Hirsch JA J Mol Biol. 2011 Aug 29. PMID:21903096<ref>PMID:21903096</ref>

-	~~===Crystal Structure~~ of ~~Rad G-domain-GTP Analog Complex===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_21903096}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3q7p]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3Q7P OCA].~~	+

	==See Also==		==See Also==
	*[[GTP-binding protein\|GTP-binding protein]]		*[[GTP-binding protein\|GTP-binding protein]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:021903096<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Hirsch, J A.]]	+	[[Category: Hirsch, J A]]
-	[[Category: Navon-Perry, L.]]	+	[[Category: Navon-Perry, L]]
-	[[Category: Sasson, Y.]]	+	[[Category: Sasson, Y]]
	[[Category: Cav2 beta]]		[[Category: Cav2 beta]]
	[[Category: G-domain]]		[[Category: G-domain]]
	[[Category: G-protein]]		[[Category: G-protein]]
	[[Category: Signaling protein]]		[[Category: Signaling protein]]

OCA at 14:41, 20 October 2012

OCA — Sat, 20 Oct 2012 14:41:53 GMT

←Older revision		Revision as of 14:41, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3q7p]] is a 2 chain structure ~~of [[GTP-binding protein]]~~ with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q7P OCA].	+	[[3q7p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q7P OCA].

	==See Also==		==See Also==

OCA at 15:56, 26 July 2012

OCA — Thu, 26 Jul 2012 15:56:33 GMT

←Older revision		Revision as of 15:56, 26 July 2012
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	[[Image:3q7p.png\|left\|200px]]		[[Image:3q7p.png\|left\|200px]]

-	<!--
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-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
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	{{STRUCTURE_3q7p\| PDB=3q7p \| SCENE= }}		{{STRUCTURE_3q7p\| PDB=3q7p \| SCENE= }}

	===Crystal Structure of Rad G-domain-GTP Analog Complex===		===Crystal Structure of Rad G-domain-GTP Analog Complex===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21903096}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 21903096 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_21903096}}		{{ABSTRACT_PUBMED_21903096}}

	==About this Structure==		==About this Structure==
-	[[3q7p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q7P OCA].	+	[[3q7p]] is a 2 chain structure of [[GTP-binding protein]] with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q7P OCA].
		+
		+	==See Also==
		+	*[[GTP-binding protein\|GTP-binding protein]]

	==Reference==		==Reference==

OCA at 07:13, 30 October 2011

OCA — Sun, 30 Oct 2011 07:13:22 GMT

←Older revision		Revision as of 07:13, 30 October 2011
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-	[[Image:3q7p.~~jpg~~\|left\|200px]]	+	[[Image:3q7p.png\|left\|200px]]

	<!--		<!--

OCA at 05:53, 21 September 2011

OCA — Wed, 21 Sep 2011 05:53:35 GMT

←Older revision		Revision as of 05:53, 21 September 2011
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-	~~'''Unreleased structure'''~~	+	[[Image:3q7p.jpg\|left\|200px]]

-	The ~~entry 3q7p~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3q7p", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3q7p\| PDB=3q7p \| SCENE= }}

-	~~Authors: Sasson, Y., Navon~~-~~Perry, L., Hirsch, J.A.~~	+	===Crystal Structure of Rad G-domain-GTP Analog Complex===

-	~~Description~~: ~~Crystal~~ Structure ~~of Rad~~ G-domain-~~GTP Analog Complex~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21903096}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21903096 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21903096}}
		+
		+	==About this Structure==
		+	[[3q7p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q7P OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021903096</ref><references group="xtra"/>
		+	[[Category: Homo sapiens]]
		+	[[Category: Hirsch, J A.]]
		+	[[Category: Navon-Perry, L.]]
		+	[[Category: Sasson, Y.]]
		+	[[Category: Cav2 beta]]
		+	[[Category: G-domain]]
		+	[[Category: G-protein]]
		+	[[Category: Signaling protein]]

OCA at 06:44, 13 July 2011

OCA — Wed, 13 Jul 2011 06:44:50 GMT

←Older revision		Revision as of 06:44, 13 July 2011
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3q7p is ON HOLD	+	The entry 3q7p is ON HOLD until Paper Publication

-	Authors: Sasson, Y., Navon-Perry, L., Hirsch, JA	+	Authors: Sasson, Y., Navon-Perry, L., Hirsch, J.A.

	Description: Crystal Structure of Rad G-domain-GTP Analog Complex		Description: Crystal Structure of Rad G-domain-GTP Analog Complex