3q7t - Revision history

OCA at 10:46, 21 February 2024

OCA — Wed, 21 Feb 2024 10:46:20 GMT

←Older revision		Revision as of 10:46, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='3q7t' size='340' side='right'caption='[[3q7t]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='3q7t' size='340' side='right'caption='[[3q7t]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3q7t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Chlt2 Chlt2~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q7T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3Q7T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3q7t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydia_trachomatis_434/Bu Chlamydia trachomatis 434/Bu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q7T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3Q7T FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NA:SODIUM+ION'>NA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.15Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[3q7r\|3q7r]], [[3q7s\|3q7s]]~~<~~/div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">CTLon_0888 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=471472 CHLT2])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3q7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q7t OCA], [https://pdbe.org/3q7t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3q7t RCSB], [https://www.ebi.ac.uk/pdbsum/3q7t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3q7t ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3q7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q7t OCA], [https://pdbe.org/3q7t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3q7t RCSB], [https://www.ebi.ac.uk/pdbsum/3q7t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3q7t ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/CHXR_CHLT2 CHXR_CHLT2] May be a global positive regulator of transcription (PubMed:21057008). Binds a cis-acting element of its own promoter DNA sequence and is hence probably also involved in its own transcription activation (PubMed:21057008, PubMed:21775428, PubMed:24646934). The recognition sequence is 5'-WHGAWNH-N(3-5)-WHGAWNH-3', where W is A/T, H is C/A/T, N is G/C/A/T and the linker length in the middle is 3 to 5 nucleotides (PubMed:21057008).<ref>PMID:21057008</ref> <ref>PMID:21775428</ref> <ref>PMID:24646934</ref>
-	~~Typically as a result of phosphorylation, OmpR~~/~~PhoB response regulators form homodimers through a receiver domain as an integral step in transcriptional activation~~. ~~Phosphorylation stabilizes the ionic and hydrophobic interactions between monomers~~. ~~Recent studies have shown that some response regulators retain functional activity in the absence~~ of ~~phosphorylation and are termed atypical response regulators~~. ~~The two currently available receiver domain structures of atypical response regulators are very similar to their phospho~~-~~accepting homologs,~~ and ~~their propensity to form homodimers~~ is ~~generally retained. An atypical response regulator~~, ~~ChxR~~, ~~from Chlamydia trachomatis, was previously reported to form homodimers; however, the residues critical to this interaction have not been elucidated~~. ~~We hypothesize that the intra~~- ~~and intermolecular interactions involved in forming a transcriptionally competent ChxR are distinct from the canonical phosphorylation~~ (~~activation~~) ~~paradigm in the OmpR~~/~~PhoB response regulator subfamily. To test this hypothesis~~, structural and functional studies were performed on the receiver domain of ChxR. Two crystal structures of the receiver domain were solved with the recently developed method using triiodo compound I3C. These structures revealed many characteristics unique to OmpR/~~PhoB subfamily members: typical or atypical. Included was the absence of two alpha-helices present in all other OmpR~~/~~PhoB response regulators. Functional studies on various dimer interface residues demonstrated that ChxR forms relatively stable homodimers through hydrophobic interactions~~, and ~~disruption of these can be accomplished with~~ the ~~introduction of a charged residue within~~ the ~~dimer interface. A gel shift study with monomeric ChxR supports that dimerization through the receiver domain~~ is ~~critical for interaction with DNA.~~	+
-		+
-	The atypical response regulator protein ChxR has structural characteristics and dimer interface interactions that are unique within the OmpR/PhoB subfamily.,Hickey JM, Lovell S, Battaile KP, Hu L, Middaugh CR, Hefty PS J Biol Chem. 2011 Sep 16;286(37)~~:32606-16~~. ~~Epub 2011 Jul 20. PMID:21775428~~<ref>PMID:~~21775428~~</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.~~<br>	+
-	</~~div~~>	+
-	<~~div class="pdbe-citations 3q7t" style="background-color~~:~~#fffaf0;">~~</~~div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Chlt2~~]]	+	[[Category: Chlamydia trachomatis 434/Bu]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Battaile~~, K P~~]]	+	[[Category: Battaile KP]]
-	[[Category: Hefty~~, P S~~]]	+	[[Category: Hefty PS]]
-	[[Category: Hickey, J]]	+	[[Category: Hickey J]]
-	[[Category: Hu, L]]	+	[[Category: Hu L]]
-	[[Category: Lovell, S]]	+	[[Category: Lovell S]]
-	[[Category: Middaugh~~, C R]]~~	+	[[Category: Middaugh CR]]
-	~~[[Category: Chlamydia]]~~	+
-	~~[[Category: Chxr]]~~	+
-	~~[[Category: Ompr]]~~	+
-	~~[[Category: Receiver domain]]~~	+
-	~~[[Category: Transcription]]~~	+
-	~~[[Category: Transcription factor~~]]	+

OCA at 05:56, 8 June 2022

OCA — Wed, 08 Jun 2022 05:56:35 GMT

←Older revision		Revision as of 05:56, 8 June 2022
Line 1:		Line 1:

	==2.15A resolution structure (I41 Form) of the ChxR receiver domain from Chlamydia trachomatis==		==2.15A resolution structure (I41 Form) of the ChxR receiver domain from Chlamydia trachomatis==
-	<StructureSection load='3q7t' size='340' side='right' caption='[[3q7t]], [[Resolution\|resolution]] 2.15Å' scene=''>	+	<StructureSection load='3q7t' size='340' side='right'caption='[[3q7t]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3q7t]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Chlt2 Chlt2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q7T OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3Q7T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3q7t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlt2 Chlt2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q7T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3Q7T FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3q7r\|3q7r]], [[3q7s\|3q7s]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3q7r\|3q7r]], [[3q7s\|3q7s]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CTLon_0888 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=471472 CHLT2])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CTLon_0888 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=471472 CHLT2])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3q7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q7t OCA], [~~http~~://pdbe.org/3q7t PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3q7t RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3q7t PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3q7t ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3q7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q7t OCA], [https://pdbe.org/3q7t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3q7t RCSB], [https://www.ebi.ac.uk/pdbsum/3q7t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3q7t ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Chlt2]]		[[Category: Chlt2]]
		+	[[Category: Large Structures]]
	[[Category: Battaile, K P]]		[[Category: Battaile, K P]]
	[[Category: Hefty, P S]]		[[Category: Hefty, P S]]

OCA at 16:46, 4 August 2016

OCA — Thu, 04 Aug 2016 16:46:33 GMT

←Older revision		Revision as of 16:46, 4 August 2016
Line 1:		Line 1:
		+
	==2.15A resolution structure (I41 Form) of the ChxR receiver domain from Chlamydia trachomatis==		==2.15A resolution structure (I41 Form) of the ChxR receiver domain from Chlamydia trachomatis==
	<StructureSection load='3q7t' size='340' side='right' caption='[[3q7t]], [[Resolution\|resolution]] 2.15Å' scene=''>		<StructureSection load='3q7t' size='340' side='right' caption='[[3q7t]], [[Resolution\|resolution]] 2.15Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3q7t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Chlamydia_trachomatis Chlamydia trachomatis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q7T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3Q7T FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3q7t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlt2 Chlt2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q7T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3Q7T FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3q7r\|3q7r]], [[3q7s\|3q7s]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3q7r\|3q7r]], [[3q7s\|3q7s]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CTLon_0888 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~813 Chlamydia trachomatis~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CTLon_0888 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=471472 CHLT2])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3q7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q7t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3q7t RCSB], [http://www.ebi.ac.uk/pdbsum/3q7t PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3q7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q7t OCA], [http://pdbe.org/3q7t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3q7t RCSB], [http://www.ebi.ac.uk/pdbsum/3q7t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3q7t ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3q7t" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Chlamydia trachomatis~~]]	+	[[Category: Chlt2]]
	[[Category: Battaile, K P]]		[[Category: Battaile, K P]]
	[[Category: Hefty, P S]]		[[Category: Hefty, P S]]

OCA at 23:22, 3 January 2015

OCA — Sat, 03 Jan 2015 23:22:49 GMT

←Older revision		Revision as of 23:22, 3 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3q7t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydia_trachomatis Chlamydia trachomatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q7T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3Q7T FirstGlance]. <br>		<table><tr><td colspan='2'>[[3q7t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydia_trachomatis Chlamydia trachomatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q7T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3Q7T FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3q7r\|3q7r]], [[3q7s\|3q7s]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3q7r\|3q7r]], [[3q7s\|3q7s]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CTLon_0888 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=813 Chlamydia trachomatis])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CTLon_0888 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=813 Chlamydia trachomatis])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3q7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q7t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3q7t RCSB], [http://www.ebi.ac.uk/pdbsum/3q7t PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3q7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q7t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3q7t RCSB], [http://www.ebi.ac.uk/pdbsum/3q7t PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 14:
	The atypical response regulator protein ChxR has structural characteristics and dimer interface interactions that are unique within the OmpR/PhoB subfamily.,Hickey JM, Lovell S, Battaile KP, Hu L, Middaugh CR, Hefty PS J Biol Chem. 2011 Sep 16;286(37):32606-16. Epub 2011 Jul 20. PMID:21775428<ref>PMID:21775428</ref>		The atypical response regulator protein ChxR has structural characteristics and dimer interface interactions that are unique within the OmpR/PhoB subfamily.,Hickey JM, Lovell S, Battaile KP, Hu L, Middaugh CR, Hefty PS J Biol Chem. 2011 Sep 16;286(37):32606-16. Epub 2011 Jul 20. PMID:21775428<ref>PMID:21775428</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Chlamydia trachomatis]]		[[Category: Chlamydia trachomatis]]
-	[[Category: Battaile, K P.]]	+	[[Category: Battaile, K P]]
-	[[Category: Hefty, P S.]]	+	[[Category: Hefty, P S]]
-	[[Category: Hickey, J.]]	+	[[Category: Hickey, J]]
-	[[Category: Hu, L.]]	+	[[Category: Hu, L]]
-	[[Category: Lovell, S.]]	+	[[Category: Lovell, S]]
-	[[Category: Middaugh, C R.]]	+	[[Category: Middaugh, C R]]
	[[Category: Chlamydia]]		[[Category: Chlamydia]]
	[[Category: Chxr]]		[[Category: Chxr]]

OCA at 05:33, 4 June 2014

OCA — Wed, 04 Jun 2014 05:33:40 GMT

←Older revision		Revision as of 05:33, 4 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3q7t.~~png~~\|~~left~~\|~~200px~~]]	+	==2.15A resolution structure (I41 Form) of the ChxR receiver domain from Chlamydia trachomatis==
		+	<StructureSection load='3q7t' size='340' side='right' caption='[[3q7t]], [[Resolution\|resolution]] 2.15Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3q7t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydia_trachomatis Chlamydia trachomatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q7T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3Q7T FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3q7r\|3q7r]], [[3q7s\|3q7s]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CTLon_0888 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=813 Chlamydia trachomatis])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3q7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q7t OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3q7t RCSB], [http://www.ebi.ac.uk/pdbsum/3q7t PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Typically as a result of phosphorylation, OmpR/PhoB response regulators form homodimers through a receiver domain as an integral step in transcriptional activation. Phosphorylation stabilizes the ionic and hydrophobic interactions between monomers. Recent studies have shown that some response regulators retain functional activity in the absence of phosphorylation and are termed atypical response regulators. The two currently available receiver domain structures of atypical response regulators are very similar to their phospho-accepting homologs, and their propensity to form homodimers is generally retained. An atypical response regulator, ChxR, from Chlamydia trachomatis, was previously reported to form homodimers; however, the residues critical to this interaction have not been elucidated. We hypothesize that the intra- and intermolecular interactions involved in forming a transcriptionally competent ChxR are distinct from the canonical phosphorylation (activation) paradigm in the OmpR/PhoB response regulator subfamily. To test this hypothesis, structural and functional studies were performed on the receiver domain of ChxR. Two crystal structures of the receiver domain were solved with the recently developed method using triiodo compound I3C. These structures revealed many characteristics unique to OmpR/PhoB subfamily members: typical or atypical. Included was the absence of two alpha-helices present in all other OmpR/PhoB response regulators. Functional studies on various dimer interface residues demonstrated that ChxR forms relatively stable homodimers through hydrophobic interactions, and disruption of these can be accomplished with the introduction of a charged residue within the dimer interface. A gel shift study with monomeric ChxR supports that dimerization through the receiver domain is critical for interaction with DNA.

-	~~<!--~~	+	The atypical response regulator protein ChxR has structural characteristics and dimer interface interactions that are unique within the OmpR/PhoB subfamily.,Hickey JM, Lovell S, Battaile KP, Hu L, Middaugh CR, Hefty PS J Biol Chem. 2011 Sep 16;286(37):32606-16. Epub 2011 Jul 20. PMID:21775428<ref>PMID:21775428</ref>
-	The ~~line below this paragraph~~, ~~containing "STRUCTURE_3q7t"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3q7t\| PDB=3q7t \| SCENE= }}~~	+

-	~~===2.15A resolution structure (I41 Form)~~ of the ~~ChxR receiver domain from Chlamydia trachomatis===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_21775428}}, adds the Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 21775428 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_21775428}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3q7t]] is a 2 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Chlamydia_trachomatis Chlamydia trachomatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q7T OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:021775428<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Chlamydia trachomatis]]		[[Category: Chlamydia trachomatis]]
	[[Category: Battaile, K P.]]		[[Category: Battaile, K P.]]

OCA at 07:41, 2 November 2011

OCA — Wed, 02 Nov 2011 07:41:20 GMT

←Older revision		Revision as of 07:41, 2 November 2011
Line 11:		Line 11:
	===2.15A resolution structure (I41 Form) of the ChxR receiver domain from Chlamydia trachomatis===		===2.15A resolution structure (I41 Form) of the ChxR receiver domain from Chlamydia trachomatis===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21775428}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21775428 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21775428}}

	==About this Structure==		==About this Structure==
	[[3q7t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydia_trachomatis Chlamydia trachomatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q7T OCA].		[[3q7t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydia_trachomatis Chlamydia trachomatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q7T OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021775428</ref><references group="xtra"/>
	[[Category: Chlamydia trachomatis]]		[[Category: Chlamydia trachomatis]]
	[[Category: Battaile, K P.]]		[[Category: Battaile, K P.]]

OCA at 06:14, 27 July 2011

OCA — Wed, 27 Jul 2011 06:14:28 GMT

←Older revision		Revision as of 06:14, 27 July 2011
Line 1:		Line 1:
-	[[Image:3q7t.~~jpg~~\|left\|200px]]	+	[[Image:3q7t.png\|left\|200px]]

	<!--		<!--

OCA at 06:55, 20 July 2011

OCA — Wed, 20 Jul 2011 06:55:33 GMT

←Older revision		Revision as of 06:55, 20 July 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3q7t.jpg\|left\|200px]]

-	The ~~entry 3q7t~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3q7t", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3q7t\| PDB=3q7t \| SCENE= }}

-	~~Authors: Hickey, J., Lovell, S., Battaile, K.P., Hu, L., Middaugh, C.R., Hefty, P.S~~.	+	===2.15A resolution structure (I41 Form) of the ChxR receiver domain from Chlamydia trachomatis===

-	~~Description:~~ 2~~.15A resolution~~ structure ~~(I41 Form) of the ChxR receiver domain~~ from Chlamydia trachomatis	+
		+	==About this Structure==
		+	[[3q7t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydia_trachomatis Chlamydia trachomatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q7T OCA].
		+	[[Category: Chlamydia trachomatis]]
		+	[[Category: Battaile, K P.]]
		+	[[Category: Hefty, P S.]]
		+	[[Category: Hickey, J.]]
		+	[[Category: Hu, L.]]
		+	[[Category: Lovell, S.]]
		+	[[Category: Middaugh, C R.]]
		+	[[Category: Chlamydia]]
		+	[[Category: Chxr]]
		+	[[Category: Ompr]]
		+	[[Category: Receiver domain]]
		+	[[Category: Transcription]]
		+	[[Category: Transcription factor]]

OCA at 06:03, 9 March 2011

OCA — Wed, 09 Mar 2011 06:03:44 GMT

←Older revision		Revision as of 06:03, 9 March 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3q7t is ON HOLD	+	The entry 3q7t is ON HOLD until Paper Publication

	Authors: Hickey, J., Lovell, S., Battaile, K.P., Hu, L., Middaugh, C.R., Hefty, P.S.		Authors: Hickey, J., Lovell, S., Battaile, K.P., Hu, L., Middaugh, C.R., Hefty, P.S.

	Description: 2.15A resolution structure (I41 Form) of the ChxR receiver domain from Chlamydia trachomatis		Description: 2.15A resolution structure (I41 Form) of the ChxR receiver domain from Chlamydia trachomatis

OCA: Protected "3q7t" [edit=sysop:move=sysop]

OCA — Wed, 12 Jan 2011 04:51:56 GMT

Protected "3q7t" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:51, 12 January 2011