3qdq - Revision history

OCA at 11:41, 14 March 2024

OCA — Thu, 14 Mar 2024 11:41:56 GMT

←Older revision		Revision as of 11:41, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3qdq' size='340' side='right'caption='[[3qdq]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='3qdq' size='340' side='right'caption='[[3qdq]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3qdq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"clostridium_aminobutyricum"_hardman_and_stadtman "clostridium~~ aminobutyricum~~" hardman and stadtman~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QDQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QDQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3qdq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_aminobutyricum Clostridium aminobutyricum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QDQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QDQ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=COA:COENZYME+A'>COA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[3gk7\|3gk7]]~~<~~/div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">abfT ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=33953 "Clostridium aminobutyricum" Hardman and Stadtman])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qdq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qdq OCA], [https://pdbe.org/3qdq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qdq RCSB], [https://www.ebi.ac.uk/pdbsum/3qdq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qdq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qdq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qdq OCA], [https://pdbe.org/3qdq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qdq RCSB], [https://www.ebi.ac.uk/pdbsum/3qdq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qdq ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q9RM86_CLOAM Q9RM86_CLOAM]
-	Clostridium aminobutyricum ferments 4-aminobutyrate (gamma-aminobutyrate, GABA) to ammonia, acetate and butyrate via 4-hydroxybutyrate that is activated to the CoA-thioester catalyzed by 4-hydroxybutyrate CoA-transferase. Then, 4-hydroxybutyryl-CoA is dehydrated to crotonyl-CoA, which disproportionates to butyryl-CoA and acetyl-CoA. Cocrystallization of the CoA-transferase with the alternate substrate butyryl-CoA yielded crystals with non-covalently bound CoA and two water molecules at the active site. Most likely, butyryl-CoA reacted with the active site Glu238 to CoA and the mixed anhydride, which slowly hydrolyzed during crystallization. The structure of the CoA is similar but less stretched than that of the CoA-moiety of the covalent enzyme-CoA-thioester in 4-hydroxybutyrate CoA-transferase from Shewanella oneidensis. In contrast to the structures of the apo-enzyme and enzyme-CoA-thioester, the structure described here has a closed conformation, probably caused by a flip of the active site loop (residues 215-219). During turnover, the closed conformation may protect the anhydride intermediate from hydrolysis and CoA from dissociation from the enzyme. Hence, one catalytic cycle changes conformation of the enzyme four times: ~~free enzyme-open conformation, CoA+ anhydride 1-closed, enzyme-CoA-thioester-open, CoA + anhydride-2-closed, free enzyme-open.~~	+
-		+
-	Crystal structure of the complex between 4-hydroxybutyrate CoA-transferase from Clostridium aminobutyricum and CoA.,Macieira S, Zhang J, Buckel W, Messerschmidt A Arch Microbiol. 2011 Aug 11. PMID:21833509<ref>PMID:21833509</~~ref>~~	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3qdq" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Clostridium aminobutyricum ~~hardman and stadtman~~]]	+	[[Category: Clostridium aminobutyricum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Macieira, S]]	+	[[Category: Macieira S]]
-	[[Category: Messerschmidt, A]]	+	[[Category: Messerschmidt A]]
-	~~[[Category: Coa]]~~	+
-	~~[[Category: Coa transferase family i]]~~	+
-	~~[[Category: Single subunit coa transferase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 06:03, 8 June 2022

OCA — Wed, 08 Jun 2022 06:03:54 GMT

←Older revision		Revision as of 06:03, 8 June 2022
Line 1:		Line 1:

	==Complex between 4-hydroxybutyrate CoA-transferase from Clostridium aminobutyricum and CoA==		==Complex between 4-hydroxybutyrate CoA-transferase from Clostridium aminobutyricum and CoA==
-	<StructureSection load='3qdq' size='340' side='right' caption='[[3qdq]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='3qdq' size='340' side='right'caption='[[3qdq]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3qdq]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"clostridium_aminobutyricum"_hardman_and_stadtman "clostridium aminobutyricum" hardman and stadtman]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QDQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3QDQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3qdq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"clostridium_aminobutyricum"_hardman_and_stadtman "clostridium aminobutyricum" hardman and stadtman]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QDQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QDQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gk7\|3gk7]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3gk7\|3gk7]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">abfT ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=33953 "Clostridium aminobutyricum" Hardman and Stadtman])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">abfT ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=33953 "Clostridium aminobutyricum" Hardman and Stadtman])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3qdq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qdq OCA], [~~http~~://pdbe.org/3qdq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3qdq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3qdq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3qdq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qdq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qdq OCA], [https://pdbe.org/3qdq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qdq RCSB], [https://www.ebi.ac.uk/pdbsum/3qdq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qdq ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Clostridium aminobutyricum hardman and stadtman]]		[[Category: Clostridium aminobutyricum hardman and stadtman]]
		+	[[Category: Large Structures]]
	[[Category: Macieira, S]]		[[Category: Macieira, S]]
	[[Category: Messerschmidt, A]]		[[Category: Messerschmidt, A]]

OCA at 23:07, 4 August 2016

OCA — Thu, 04 Aug 2016 23:07:31 GMT

←Older revision		Revision as of 23:07, 4 August 2016
Line 1:		Line 1:
		+
	==Complex between 4-hydroxybutyrate CoA-transferase from Clostridium aminobutyricum and CoA==		==Complex between 4-hydroxybutyrate CoA-transferase from Clostridium aminobutyricum and CoA==
	<StructureSection load='3qdq' size='340' side='right' caption='[[3qdq]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='3qdq' size='340' side='right' caption='[[3qdq]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3qdq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Clostridium_aminobutyricum Clostridium~~ aminobutyricum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QDQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QDQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3qdq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"clostridium_aminobutyricum"_hardman_and_stadtman "clostridium aminobutyricum" hardman and stadtman]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QDQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QDQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gk7\|3gk7]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gk7\|3gk7]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">abfT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=33953 Clostridium aminobutyricum])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">abfT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=33953 "Clostridium aminobutyricum" Hardman and Stadtman])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qdq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qdq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qdq RCSB], [http://www.ebi.ac.uk/pdbsum/3qdq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qdq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qdq OCA], [http://pdbe.org/3qdq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3qdq RCSB], [http://www.ebi.ac.uk/pdbsum/3qdq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3qdq ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3qdq" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Clostridium aminobutyricum]]	+	[[Category: Clostridium aminobutyricum hardman and stadtman]]
	[[Category: Macieira, S]]		[[Category: Macieira, S]]
	[[Category: Messerschmidt, A]]		[[Category: Messerschmidt, A]]

OCA at 22:53, 3 January 2015

OCA — Sat, 03 Jan 2015 22:53:37 GMT

←Older revision		Revision as of 22:53, 3 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3qdq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_aminobutyricum Clostridium aminobutyricum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QDQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QDQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[3qdq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_aminobutyricum Clostridium aminobutyricum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QDQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QDQ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gk7\|3gk7]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gk7\|3gk7]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">abfT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=33953 Clostridium aminobutyricum])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">abfT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=33953 Clostridium aminobutyricum])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qdq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qdq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qdq RCSB], [http://www.ebi.ac.uk/pdbsum/3qdq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qdq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qdq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qdq RCSB], [http://www.ebi.ac.uk/pdbsum/3qdq PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 14:
	Crystal structure of the complex between 4-hydroxybutyrate CoA-transferase from Clostridium aminobutyricum and CoA.,Macieira S, Zhang J, Buckel W, Messerschmidt A Arch Microbiol. 2011 Aug 11. PMID:21833509<ref>PMID:21833509</ref>		Crystal structure of the complex between 4-hydroxybutyrate CoA-transferase from Clostridium aminobutyricum and CoA.,Macieira S, Zhang J, Buckel W, Messerschmidt A Arch Microbiol. 2011 Aug 11. PMID:21833509<ref>PMID:21833509</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Clostridium aminobutyricum]]		[[Category: Clostridium aminobutyricum]]
-	[[Category: Macieira, S.]]	+	[[Category: Macieira, S]]
-	[[Category: Messerschmidt, A.]]	+	[[Category: Messerschmidt, A]]
	[[Category: Coa]]		[[Category: Coa]]
	[[Category: Coa transferase family i]]		[[Category: Coa transferase family i]]
	[[Category: Single subunit coa transferase]]		[[Category: Single subunit coa transferase]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 05:31, 4 June 2014

OCA — Wed, 04 Jun 2014 05:31:56 GMT

←Older revision		Revision as of 05:31, 4 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3qdq.~~png~~\|~~left~~\|~~200px~~]]	+	==Complex between 4-hydroxybutyrate CoA-transferase from Clostridium aminobutyricum and CoA==
		+	<StructureSection load='3qdq' size='340' side='right' caption='[[3qdq]], [[Resolution\|resolution]] 2.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3qdq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_aminobutyricum Clostridium aminobutyricum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QDQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QDQ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3gk7\|3gk7]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">abfT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=33953 Clostridium aminobutyricum])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qdq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qdq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qdq RCSB], [http://www.ebi.ac.uk/pdbsum/3qdq PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Clostridium aminobutyricum ferments 4-aminobutyrate (gamma-aminobutyrate, GABA) to ammonia, acetate and butyrate via 4-hydroxybutyrate that is activated to the CoA-thioester catalyzed by 4-hydroxybutyrate CoA-transferase. Then, 4-hydroxybutyryl-CoA is dehydrated to crotonyl-CoA, which disproportionates to butyryl-CoA and acetyl-CoA. Cocrystallization of the CoA-transferase with the alternate substrate butyryl-CoA yielded crystals with non-covalently bound CoA and two water molecules at the active site. Most likely, butyryl-CoA reacted with the active site Glu238 to CoA and the mixed anhydride, which slowly hydrolyzed during crystallization. The structure of the CoA is similar but less stretched than that of the CoA-moiety of the covalent enzyme-CoA-thioester in 4-hydroxybutyrate CoA-transferase from Shewanella oneidensis. In contrast to the structures of the apo-enzyme and enzyme-CoA-thioester, the structure described here has a closed conformation, probably caused by a flip of the active site loop (residues 215-219). During turnover, the closed conformation may protect the anhydride intermediate from hydrolysis and CoA from dissociation from the enzyme. Hence, one catalytic cycle changes conformation of the enzyme four times: free enzyme-open conformation, CoA+ anhydride 1-closed, enzyme-CoA-thioester-open, CoA + anhydride-2-closed, free enzyme-open.

-	<!--	+	Crystal structure of the complex between 4-hydroxybutyrate CoA-transferase from Clostridium aminobutyricum and CoA.,Macieira S, Zhang J, Buckel W, Messerschmidt A Arch Microbiol. 2011 Aug 11. PMID:21833509<ref>PMID:21833509</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3qdq"~~, ~~creates the "Structure Box" on the page.~~	+
-	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded)~~,	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3qdq\| PDB=3qdq \| SCENE= }}~~	+

-	~~===Complex between 4-hydroxybutyrate CoA-transferase from Clostridium aminobutyricum and CoA===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph~~, ~~{{ABSTRACT_PUBMED_21833509}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 21833509 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_21833509}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3qdq]] is a 1 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Clostridium_aminobutyricum Clostridium aminobutyricum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QDQ OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:021833509<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Clostridium aminobutyricum]]		[[Category: Clostridium aminobutyricum]]
	[[Category: Macieira, S.]]		[[Category: Macieira, S.]]

OCA at 07:56, 29 February 2012

OCA — Wed, 29 Feb 2012 07:56:28 GMT

←Older revision		Revision as of 07:56, 29 February 2012
Line 1:		Line 1:
-	[[Image:3qdq.~~jpg~~\|left\|200px]]	+	[[Image:3qdq.png\|left\|200px]]

	<!--		<!--

OCA at 07:31, 24 August 2011

OCA — Wed, 24 Aug 2011 07:31:47 GMT

←Older revision		Revision as of 07:31, 24 August 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3qdq.jpg\|left\|200px]]

-	The ~~entry 3qdq~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3qdq", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3qdq\| PDB=3qdq \| SCENE= }}

-	~~Authors: Messerschmidt, A., Macieira, S.~~	+	===Complex between 4-hydroxybutyrate CoA-transferase from Clostridium aminobutyricum and CoA===

-	~~Description~~: ~~Complex between 4~~-~~hydroxybutyrate CoA~~-~~transferase~~ from Clostridium aminobutyricum ~~and CoA~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21833509}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21833509 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21833509}}
		+
		+	==About this Structure==
		+	[[3qdq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_aminobutyricum Clostridium aminobutyricum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QDQ OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021833509</ref><references group="xtra"/>
		+	[[Category: Clostridium aminobutyricum]]
		+	[[Category: Macieira, S.]]
		+	[[Category: Messerschmidt, A.]]
		+	[[Category: Coa]]
		+	[[Category: Coa transferase family i]]
		+	[[Category: Single subunit coa transferase]]
		+	[[Category: Transferase]]

OCA at 06:04, 9 March 2011

OCA — Wed, 09 Mar 2011 06:04:30 GMT

←Older revision		Revision as of 06:04, 9 March 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3qdq is ON HOLD	+	The entry 3qdq is ON HOLD until Paper Publication

	Authors: Messerschmidt, A., Macieira, S.		Authors: Messerschmidt, A., Macieira, S.

	Description: Complex between 4-hydroxybutyrate CoA-transferase from Clostridium aminobutyricum and CoA		Description: Complex between 4-hydroxybutyrate CoA-transferase from Clostridium aminobutyricum and CoA

OCA: Protected "3qdq" [edit=sysop:move=sysop]

OCA — Wed, 26 Jan 2011 12:46:57 GMT

Protected "3qdq" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:46, 26 January 2011

OCA: New page: '''Unreleased structure''' The entry 3qdq is ON HOLD Authors: Messerschmidt, A., Macieira, S. Description: Complex between 4-hydroxybutyrate CoA-transferase from Clostridium aminobutyr...

OCA — Wed, 26 Jan 2011 12:46:56 GMT

New page: '''Unreleased structure''' The entry 3qdq is ON HOLD Authors: Messerschmidt, A., Macieira, S. Description: Complex between 4-hydroxybutyrate CoA-transferase from Clostridium aminobutyr...

New page

'''Unreleased structure'''

The entry 3qdq is ON HOLD

Authors: Messerschmidt, A., Macieira, S.

Description: Complex between 4-hydroxybutyrate CoA-transferase from Clostridium aminobutyricum and CoA