3qho - Revision history

OCA at 17:12, 1 November 2023

OCA — Wed, 01 Nov 2023 17:12:57 GMT

←Older revision		Revision as of 17:12, 1 November 2023
Line 3:		Line 3:
	<StructureSection load='3qho' size='340' side='right'caption='[[3qho]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='3qho' size='340' side='right'caption='[[3qho]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3qho]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~'pyrococcus_shinkaii' 'pyrococcus shinkaii'~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QHO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QHO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3qho]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QHO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QHO FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=BGC:BETA~~-~~D-GLUCOSE'>BGC</scene>~~, ~~<scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.65Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[3qhm\|3qhm]], [[3qhn\|3qhn]], [[3axx\|3axx]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=PRD_900011:beta-cellotetraose'>PRD_900011</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b></td><td class="sblockDat">PH1171 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953~~ '~~Pyrococcus shinkaii'])</td~~></tr>	+
-	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qho FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qho OCA], [https://pdbe.org/3qho PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qho RCSB], [https://www.ebi.ac.uk/pdbsum/3qho PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qho ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qho FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qho OCA], [https://pdbe.org/3qho PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qho RCSB], [https://www.ebi.ac.uk/pdbsum/3qho PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qho ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/O58925_PYRHO O58925_PYRHO]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Pyrococcus shinkaii]]
-	[[Category: Cellulase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Ishikawa, K~~]]	+	[[Category: Pyrococcus horikoshii]]
-	[[Category: ~~Kim, H W~~]]	+	[[Category: Ishikawa K]]
-	[[Category: ~~Catalytic domain]]~~	+	[[Category: Kim H-W]]
-	~~[[Category: Endoglucanase]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 06:09, 8 June 2022

OCA — Wed, 08 Jun 2022 06:09:15 GMT

←Older revision		Revision as of 06:09, 8 June 2022
Line 1:		Line 1:

	==Crystal analysis of the complex structure, Y299F-cellotetraose, of endocellulase from pyrococcus horikoshii==		==Crystal analysis of the complex structure, Y299F-cellotetraose, of endocellulase from pyrococcus horikoshii==
-	<StructureSection load='3qho' size='340' side='right' caption='[[3qho]], [[Resolution\|resolution]] 1.65Å' scene=''>	+	<StructureSection load='3qho' size='340' side='right'caption='[[3qho]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3qho]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/'pyrococcus_shinkaii' 'pyrococcus shinkaii']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QHO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3QHO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3qho]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/'pyrococcus_shinkaii' 'pyrococcus shinkaii']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QHO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QHO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~CTT~~:BETA-D-~~GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSE~~'>~~CTT~~</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qhm\|3qhm]], [[3qhn\|3qhn]], [[3axx\|3axx]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3qhm\|3qhm]], [[3qhn\|3qhn]], [[3axx\|3axx]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PH1171 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 'Pyrococcus shinkaii'])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PH1171 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 'Pyrococcus shinkaii'])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3qho FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qho OCA], [~~http~~://pdbe.org/3qho PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3qho RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3qho PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3qho ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qho FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qho OCA], [https://pdbe.org/3qho PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qho RCSB], [https://www.ebi.ac.uk/pdbsum/3qho PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qho ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 25:		Line 25:
	[[Category: Pyrococcus shinkaii]]		[[Category: Pyrococcus shinkaii]]
	[[Category: Cellulase]]		[[Category: Cellulase]]
		+	[[Category: Large Structures]]
	[[Category: Ishikawa, K]]		[[Category: Ishikawa, K]]
	[[Category: Kim, H W]]		[[Category: Kim, H W]]

OCA at 15:05, 4 August 2016

OCA — Thu, 04 Aug 2016 15:05:55 GMT

←Older revision		Revision as of 15:05, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal analysis of the complex structure, Y299F-cellotetraose, of endocellulase from pyrococcus horikoshii==		==Crystal analysis of the complex structure, Y299F-cellotetraose, of endocellulase from pyrococcus horikoshii==
	<StructureSection load='3qho' size='340' side='right' caption='[[3qho]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='3qho' size='340' side='right' caption='[[3qho]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3qho]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pyrococcus_horikoshii Pyrococcus horikoshii~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QHO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QHO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3qho]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/'pyrococcus_shinkaii' 'pyrococcus shinkaii']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QHO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QHO FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CTT:BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSE'>CTT</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CTT:BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSE'>CTT</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qhm\|3qhm]], [[3qhn\|3qhn]], [[3axx\|3axx]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qhm\|3qhm]], [[3qhn\|3qhn]], [[3axx\|3axx]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PH1171 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 Pyrococcus ~~horikoshii~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PH1171 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 'Pyrococcus shinkaii'])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qho FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qho OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qho RCSB], [http://www.ebi.ac.uk/pdbsum/3qho PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qho FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qho OCA], [http://pdbe.org/3qho PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3qho RCSB], [http://www.ebi.ac.uk/pdbsum/3qho PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3qho ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3qho" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Pyrococcus shinkaii]]
	[[Category: Cellulase]]		[[Category: Cellulase]]
-	[[Category: Pyrococcus horikoshii]]
	[[Category: Ishikawa, K]]		[[Category: Ishikawa, K]]
	[[Category: Kim, H W]]		[[Category: Kim, H W]]

OCA at 21:40, 3 January 2015

OCA — Sat, 03 Jan 2015 21:40:53 GMT

←Older revision		Revision as of 21:40, 3 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3qho]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QHO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QHO FirstGlance]. <br>		<table><tr><td colspan='2'>[[3qho]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QHO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QHO FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CTT:BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSE'>CTT</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CTT:BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSE'>CTT</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qhm\|3qhm]], [[3qhn\|3qhn]], [[3axx\|3axx]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qhm\|3qhm]], [[3qhn\|3qhn]], [[3axx\|3axx]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PH1171 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 Pyrococcus horikoshii])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PH1171 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 Pyrococcus horikoshii])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qho FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qho OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qho RCSB], [http://www.ebi.ac.uk/pdbsum/3qho PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qho FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qho OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qho RCSB], [http://www.ebi.ac.uk/pdbsum/3qho PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	[[Category: Cellulase]]		[[Category: Cellulase]]
	[[Category: Pyrococcus horikoshii]]		[[Category: Pyrococcus horikoshii]]
-	[[Category: Ishikawa, K.]]	+	[[Category: Ishikawa, K]]
-	[[Category: Kim, H W.]]	+	[[Category: Kim, H W]]
	[[Category: Catalytic domain]]		[[Category: Catalytic domain]]
-	[[Category: Cellulase]]
	[[Category: Endoglucanase]]		[[Category: Endoglucanase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 07:00, 9 June 2014

OCA — Mon, 09 Jun 2014 07:00:08 GMT

←Older revision		Revision as of 07:00, 9 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3qho.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal analysis of the complex structure, Y299F-cellotetraose, of endocellulase from pyrococcus horikoshii==
		+	<StructureSection load='3qho' size='340' side='right' caption='[[3qho]], [[Resolution\|resolution]] 1.65Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3qho]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QHO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QHO FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CTT:BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSYL-(1- 4)-BETA-D-GLUCOPYRANOSE'>CTT</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qhm\|3qhm]], [[3qhn\|3qhn]], [[3axx\|3axx]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PH1171 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 Pyrococcus horikoshii])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qho FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qho OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qho RCSB], [http://www.ebi.ac.uk/pdbsum/3qho PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A hyperthermophilic membrane-related beta-1,4-endoglucanase (family 5, cellulase) of the archaeon Pyrococcus horikoshii was found to be capable of hydrolysing cellulose at high temperatures. The hyperthermophilic cellulase has promise for applications in biomass utilization. To clarify its detailed function, we determined the crystal structures of mutants of the enzyme in complex with either the substrate or product ligands. We were able to resolve different kinds of complex structures at 1.65-2.01 A (1 A=0.1 nm). The structural analysis of various mutant enzymes yielded a sequence of crystallographic snapshots, which could be used to explain the catalytic process of the enzyme. The substrate position is fixed by the alignment of one cellobiose unit between the two aromatic amino acid residues at subsites +1 and +2. During the enzyme reaction, the glucose structure of cellulose substrates is distorted at subsite -1, and the beta-1,4-glucoside bond between glucose moieties is twisted between subsites -1 and +1. Subsite -2 specifically recognizes the glucose residue, but recognition by subsites +1 and +2 is loose during the enzyme reaction. This type of recognition is important for creation of the distorted boat form of the substrate at subsite -1. A rare enzyme-substrate complex was observed within the low-activity mutant Y299F, which suggested the existence of a trapped ligand structure before the formation by covalent bonding of the proposed intermediate structure. Analysis of the enzyme-substrate structure suggested that an incoming water molecule, essential for hydrolysis during the retention process, might be introduced to the cleavage position after the cellobiose product at subsites +1 and +2 was released from the active site.

-	~~{{STRUCTURE_3qho\| PDB=3qho \| SCENE= }}~~	+	Functional analysis of hyperthermophilic endocellulase from Pyrococcus horikoshii by crystallographic snapshots.,Kim HW, Ishikawa K Biochem J. 2011 Jul 15;437(2):223-30. PMID:21557724<ref>PMID:21557724</ref>

-	~~===Crystal analysis~~ of the ~~complex structure, Y299F-cellotetraose, of endocellulase from pyrococcus horikoshii===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_21557724}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3qho]] is a 3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3QHO OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:021557724<~~/~~ref~~><~~ref group="xtra">PMID:019052378</ref><ref group="xtra">PMID:019847920</ref><references group="xtra"~~/>	+
	[[Category: Cellulase]]		[[Category: Cellulase]]
	[[Category: Pyrococcus horikoshii]]		[[Category: Pyrococcus horikoshii]]

OCA at 10:28, 8 August 2012

OCA — Wed, 08 Aug 2012 10:28:49 GMT

←Older revision		Revision as of 10:28, 8 August 2012
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-	[[Image:3qho.~~jpg~~\|left\|200px]]	+	[[Image:3qho.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3qho", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3qho\| PDB=3qho \| SCENE= }}		{{STRUCTURE_3qho\| PDB=3qho \| SCENE= }}

	===Crystal analysis of the complex structure, Y299F-cellotetraose, of endocellulase from pyrococcus horikoshii===		===Crystal analysis of the complex structure, Y299F-cellotetraose, of endocellulase from pyrococcus horikoshii===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21557724}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 21557724 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_21557724}}		{{ABSTRACT_PUBMED_21557724}}

OCA at 07:24, 1 February 2012

OCA — Wed, 01 Feb 2012 07:24:11 GMT

←Older revision		Revision as of 07:24, 1 February 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3qho.jpg\|left\|200px]]

-	The ~~entry 3qho~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3qho", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3qho\| PDB=3qho \| SCENE= }}

-	~~Authors: Kim~~, H.-W., ~~Ishikawa, K.~~	+	===Crystal analysis of the complex structure, Y299F-cellotetraose, of endocellulase from pyrococcus horikoshii===

-	~~Description: Crystal analysis of~~ the ~~complex structure~~, ~~Y299F~~-~~cellotetraose, of endocellulase~~ from ~~pyrococcus~~ horikoshii	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21557724}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21557724 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21557724}}
		+
		+	==About this Structure==
		+	[[3qho]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QHO OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021557724</ref><ref group="xtra">PMID:019052378</ref><ref group="xtra">PMID:019847920</ref><references group="xtra"/>
		+	[[Category: Cellulase]]
		+	[[Category: Pyrococcus horikoshii]]
		+	[[Category: Ishikawa, K.]]
		+	[[Category: Kim, H W.]]
		+	[[Category: Catalytic domain]]
		+	[[Category: Cellulase]]
		+	[[Category: Endoglucanase]]
		+	[[Category: Hydrolase]]

OCA at 04:54, 13 April 2011

OCA — Wed, 13 Apr 2011 04:54:59 GMT

←Older revision		Revision as of 04:54, 13 April 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3qho is ON HOLD	+	The entry 3qho is ON HOLD until Paper Publication

	Authors: Kim, H.-W., Ishikawa, K.		Authors: Kim, H.-W., Ishikawa, K.

	Description: Crystal analysis of the complex structure, Y299F-cellotetraose, of endocellulase from pyrococcus horikoshii		Description: Crystal analysis of the complex structure, Y299F-cellotetraose, of endocellulase from pyrococcus horikoshii

OCA at 05:49, 9 February 2011

OCA — Wed, 09 Feb 2011 05:49:50 GMT

←Older revision		Revision as of 05:49, 9 February 2011
Line 5:		Line 5:
	Authors: Kim, H.-W., Ishikawa, K.		Authors: Kim, H.-W., Ishikawa, K.

-	Description: ~~CRYSTAL ANALYSIS OF THE COMPLEX STRUCTURE~~, Y299F-~~CELLOTETRAOSE~~, ~~OF ENDOCELLULASE FROM PYROCOCCUS HORIKOSHII~~	+	Description: Crystal analysis of the complex structure, Y299F-cellotetraose, of endocellulase from pyrococcus horikoshii

OCA: Protected "3qho" [edit=sysop:move=sysop]

OCA — Wed, 02 Feb 2011 06:12:57 GMT

Protected "3qho" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:12, 2 February 2011