3qhy - Revision history

OCA at 11:46, 14 March 2024

OCA — Thu, 14 Mar 2024 11:46:08 GMT

←Older revision		Revision as of 11:46, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3qhy' size='340' side='right'caption='[[3qhy]], [[Resolution\|resolution]] 2.06Å' scene=''>		<StructureSection load='3qhy' size='340' side='right'caption='[[3qhy]], [[Resolution\|resolution]] 2.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3qhy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"actinomyces_exfoliatus"_waksman_and_curtis_1916 "actinomyces exfoliatus" waksman and curtis 1916~~] and [https://en.wikipedia.org/wiki/~~"bacillus_cereus_var._anthracis"_(cohn_1872)_smith_et_al._1946 "bacillus cereus var. anthracis" (cohn 1872) smith et al. 1946~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QHY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QHY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3qhy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis] and [https://en.wikipedia.org/wiki/Streptomyces_exfoliatus Streptomyces exfoliatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QHY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QHY FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[1jtd\|1jtd]], [[1jtg\|1jtg]], [[3qi0\|3qi0]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.06Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">bla1, BA_2507, GBAA2507, GBAA_2507 ([https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&srchmode=5&id=1392 "Bacillus cereus var. anthracis" (Cohn 1872) Smith et al. 1946]), bliB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1905 "Actinomyces exfoliatus" Waksman and Curtis 1916])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qhy OCA], [https://pdbe.org/3qhy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qhy RCSB], [https://www.ebi.ac.uk/pdbsum/3qhy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qhy ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qhy OCA], [https://pdbe.org/3qhy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qhy RCSB], [https://www.ebi.ac.uk/pdbsum/3qhy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qhy ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q93T42_BACAN Q93T42_BACAN]
-	beta-Lactamases hydrolyze beta-lactam antibiotics to provide drug resistance to bacteria. beta-Lactamase inhibitory protein-II (BLIP-II) is a potent proteinaceous inhibitor that exhibits low picomolar affinity for class A beta-lactamases. This study examines the driving forces for binding between BLIP-II and beta-lactamases using a combination of presteady state kinetics, isothermal titration calorimetry, and x-ray crystallography. The measured dissociation rate constants for BLIP-II and various beta-lactamases ranged from 10(-4) to 10(-7) s(-1) and are comparable with those found in some of the tightest known protein-protein interactions. The crystal structures of BLIP-II alone and in complex with Bacillus anthracis Bla1 beta-lactamase revealed no significant side-chain movement in BLIP-II in the complex versus the monomer. The structural rigidity of BLIP-II minimizes the loss of the entropy upon complex formation and, as indicated by thermodynamics experiments, may be a key determinant of the observed potent inhibition of beta-lactamases.	+
-		+
-	Analysis of the binding forces driving the tight interactions between beta-lactamase inhibitory protein-II (BLIP-II) and class A beta-lactamases.,Brown NG, Chow DC, Sankaran B, Zwart P, Prasad BV, Palzkill T J Biol Chem. 2011 Sep 16;286(37):~~32723-35. Epub 2011 Jul 20. PMID:21775426<ref>PMID:21775426<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3qhy" style="background-color:#fffaf0;"><~~/~~div>~~	+

	==See Also==		==See Also==
	*[[Beta-lactamase 3D structures\|Beta-lactamase 3D structures]]		*[[Beta-lactamase 3D structures\|Beta-lactamase 3D structures]]
	*[[TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)\|TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)]]		*[[TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)\|TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Actinomyces exfoliatus waksman and curtis 1916]]~~	+	[[Category: Bacillus anthracis]]
-	~~[[Category: Beta-lactamase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Structural genomic~~]]	+	[[Category: Streptomyces exfoliatus]]
-	[[Category: Brown~~, N G~~]]	+	[[Category: Brown NG]]
-	[[Category: Chow~~, D C~~]]	+	[[Category: Chow DC]]
-	[[Category: Palzkill, T]]	+	[[Category: Palzkill T]]
-	[[Category: Prasad~~, B V.V~~]]	+	[[Category: Prasad BVV]]
-	[[Category: Sankaran, B]]	+	[[Category: Sankaran B]]
-	[[Category: Zwart, P]]	+	[[Category: Zwart P]]
-	~~[[Category: Beta-propeller]]~~	+
-	~~[[Category: Enyzme-inhibitor complex]]~~	+
-	~~[[Category: Hydrolase-hydrolase inhibitor complex]]~~	+
-	~~[[Category: Protein:protein interaction~~]]	+

OCA at 06:09, 8 June 2022

OCA — Wed, 08 Jun 2022 06:09:35 GMT

←Older revision		Revision as of 06:09, 8 June 2022
Line 1:		Line 1:

	==Structural, thermodynamic and kinetic analysis of the picomolar binding affinity interaction of the beta-lactamase inhibitor protein-II (BLIP-II) with class A beta-lactamases==		==Structural, thermodynamic and kinetic analysis of the picomolar binding affinity interaction of the beta-lactamase inhibitor protein-II (BLIP-II) with class A beta-lactamases==
-	<StructureSection load='3qhy' size='340' side='right' caption='[[3qhy]], [[Resolution\|resolution]] 2.06Å' scene=''>	+	<StructureSection load='3qhy' size='340' side='right'caption='[[3qhy]], [[Resolution\|resolution]] 2.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3qhy]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"actinomyces_exfoliatus"_waksman_and_curtis_1916 "actinomyces exfoliatus" waksman and curtis 1916] and [~~http~~://en.wikipedia.org/wiki/"bacillus_cereus_var._anthracis"_(cohn_1872)_smith_et_al._1946 "bacillus cereus var. anthracis" (cohn 1872) smith et al. 1946]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QHY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3QHY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3qhy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"actinomyces_exfoliatus"_waksman_and_curtis_1916 "actinomyces exfoliatus" waksman and curtis 1916] and [https://en.wikipedia.org/wiki/"bacillus_cereus_var._anthracis"_(cohn_1872)_smith_et_al._1946 "bacillus cereus var. anthracis" (cohn 1872) smith et al. 1946]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QHY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QHY FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jtd\|1jtd]], [[1jtg\|1jtg]], [[3qi0\|3qi0]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1jtd\|1jtd]], [[1jtg\|1jtg]], [[3qi0\|3qi0]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bla1, BA_2507, GBAA2507, GBAA_2507 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 "Bacillus cereus var. anthracis" (Cohn 1872) Smith et al. 1946]), bliB ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1905 "Actinomyces exfoliatus" Waksman and Curtis 1916])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bla1, BA_2507, GBAA2507, GBAA_2507 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 "Bacillus cereus var. anthracis" (Cohn 1872) Smith et al. 1946]), bliB ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1905 "Actinomyces exfoliatus" Waksman and Curtis 1916])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3qhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qhy OCA], [~~http~~://pdbe.org/3qhy PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3qhy RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3qhy PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3qhy ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qhy OCA], [https://pdbe.org/3qhy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qhy RCSB], [https://www.ebi.ac.uk/pdbsum/3qhy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qhy ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 20:

	==See Also==		==See Also==
-	*[[Beta-lactamase\|Beta-lactamase]]	+	*[[Beta-lactamase 3D structures\|Beta-lactamase 3D structures]]
	*[[TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)\|TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)]]		*[[TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)\|TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)]]
	== References ==		== References ==
Line 28:		Line 28:
	[[Category: Actinomyces exfoliatus waksman and curtis 1916]]		[[Category: Actinomyces exfoliatus waksman and curtis 1916]]
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
		+	[[Category: Large Structures]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]
	[[Category: Brown, N G]]		[[Category: Brown, N G]]

OCA at 11:24, 10 December 2016

OCA — Sat, 10 Dec 2016 11:24:36 GMT

←Older revision		Revision as of 11:24, 10 December 2016
Line 1:		Line 1:
		+
	==Structural, thermodynamic and kinetic analysis of the picomolar binding affinity interaction of the beta-lactamase inhibitor protein-II (BLIP-II) with class A beta-lactamases==		==Structural, thermodynamic and kinetic analysis of the picomolar binding affinity interaction of the beta-lactamase inhibitor protein-II (BLIP-II) with class A beta-lactamases==
	<StructureSection load='3qhy' size='340' side='right' caption='[[3qhy]], [[Resolution\|resolution]] 2.06Å' scene=''>		<StructureSection load='3qhy' size='340' side='right' caption='[[3qhy]], [[Resolution\|resolution]] 2.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3qhy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_anthracis Bacillus anthracis~~] and [http://en.wikipedia.org/wiki/~~Streptomyces_exfoliatus Streptomyces exfoliatus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QHY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QHY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3qhy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_exfoliatus"_waksman_and_curtis_1916 "actinomyces exfoliatus" waksman and curtis 1916] and [http://en.wikipedia.org/wiki/"bacillus_cereus_var._anthracis"_(cohn_1872)_smith_et_al._1946 "bacillus cereus var. anthracis" (cohn 1872) smith et al. 1946]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QHY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QHY FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jtd\|1jtd]], [[1jtg\|1jtg]], [[3qi0\|3qi0]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jtd\|1jtd]], [[1jtg\|1jtg]], [[3qi0\|3qi0]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bla1, BA_2507, GBAA2507, GBAA_2507 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 Bacillus anthracis]), bliB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1905 ~~Streptomyces~~ exfoliatus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bla1, BA_2507, GBAA2507, GBAA_2507 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 "Bacillus cereus var. anthracis" (Cohn 1872) Smith et al. 1946]), bliB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1905 "Actinomyces exfoliatus" Waksman and Curtis 1916])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qhy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qhy RCSB], [http://www.ebi.ac.uk/pdbsum/3qhy PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qhy OCA], [http://pdbe.org/3qhy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3qhy RCSB], [http://www.ebi.ac.uk/pdbsum/3qhy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3qhy ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3qhy" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 24:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus anthracis~~]]	+	[[Category: Actinomyces exfoliatus waksman and curtis 1916]]
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
-	[[Category: Streptomyces exfoliatus]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]
	[[Category: Brown, N G]]		[[Category: Brown, N G]]

OCA at 11:58, 9 December 2014

OCA — Tue, 09 Dec 2014 11:58:46 GMT

←Older revision		Revision as of 11:58, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3qhy.~~png~~\|~~left~~\|~~200px~~]]	+	==Structural, thermodynamic and kinetic analysis of the picomolar binding affinity interaction of the beta-lactamase inhibitor protein-II (BLIP-II) with class A beta-lactamases==
		+	<StructureSection load='3qhy' size='340' side='right' caption='[[3qhy]], [[Resolution\|resolution]] 2.06Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3qhy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis] and [http://en.wikipedia.org/wiki/Streptomyces_exfoliatus Streptomyces exfoliatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QHY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QHY FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1jtd\|1jtd]], [[1jtg\|1jtg]], [[3qi0\|3qi0]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bla1, BA_2507, GBAA2507, GBAA_2507 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 Bacillus anthracis]), bliB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1905 Streptomyces exfoliatus])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qhy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qhy RCSB], [http://www.ebi.ac.uk/pdbsum/3qhy PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	beta-Lactamases hydrolyze beta-lactam antibiotics to provide drug resistance to bacteria. beta-Lactamase inhibitory protein-II (BLIP-II) is a potent proteinaceous inhibitor that exhibits low picomolar affinity for class A beta-lactamases. This study examines the driving forces for binding between BLIP-II and beta-lactamases using a combination of presteady state kinetics, isothermal titration calorimetry, and x-ray crystallography. The measured dissociation rate constants for BLIP-II and various beta-lactamases ranged from 10(-4) to 10(-7) s(-1) and are comparable with those found in some of the tightest known protein-protein interactions. The crystal structures of BLIP-II alone and in complex with Bacillus anthracis Bla1 beta-lactamase revealed no significant side-chain movement in BLIP-II in the complex versus the monomer. The structural rigidity of BLIP-II minimizes the loss of the entropy upon complex formation and, as indicated by thermodynamics experiments, may be a key determinant of the observed potent inhibition of beta-lactamases.

-	~~{{STRUCTURE_3qhy\| PDB=3qhy \| SCENE= }}~~	+	Analysis of the binding forces driving the tight interactions between beta-lactamase inhibitory protein-II (BLIP-II) and class A beta-lactamases.,Brown NG, Chow DC, Sankaran B, Zwart P, Prasad BV, Palzkill T J Biol Chem. 2011 Sep 16;286(37):32723-35. Epub 2011 Jul 20. PMID:21775426<ref>PMID:21775426</ref>

-	~~===Structural~~, ~~thermodynamic and kinetic analysis~~ of the ~~picomolar binding affinity interaction of the beta-lactamase inhibitor protein-II (BLIP-II) with class A beta-lactamases===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_21775426}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3qhy]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bacillus_anthracis Bacillus anthracis] and [http://en~~.~~wikipedia.org~~/~~wiki/Streptomyces_exfoliatus Streptomyces exfoliatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QHY OCA].~~	+

	==See Also==		==See Also==
		+	*[[Beta-lactamase\|Beta-lactamase]]
	*[[TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)\|TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)]]		*[[TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)\|TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:021775426<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bacillus anthracis]]		[[Category: Bacillus anthracis]]
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
	[[Category: Streptomyces exfoliatus]]		[[Category: Streptomyces exfoliatus]]
-	[[Category: ~~BSGC, Berkeley~~ Structural ~~Genomics Center.~~]]	+	[[Category: Structural genomic]]
-	[[Category: Brown, N G.]]	+	[[Category: Brown, N G]]
-	[[Category: Chow, D C.]]	+	[[Category: Chow, D C]]
-	[[Category: Palzkill, T.]]	+	[[Category: Palzkill, T]]
-	[[Category: Prasad, B V.V.]]	+	[[Category: Prasad, B V.V]]
-	[[Category: Sankaran, B.]]	+	[[Category: Sankaran, B]]
-	[[Category: Zwart, P~~.]]~~	+	[[Category: Zwart, P]]
-	~~[[Category: Beta-lactamase~~]]	+
	[[Category: Beta-propeller]]		[[Category: Beta-propeller]]
	[[Category: Enyzme-inhibitor complex]]		[[Category: Enyzme-inhibitor complex]]
	[[Category: Hydrolase-hydrolase inhibitor complex]]		[[Category: Hydrolase-hydrolase inhibitor complex]]
	[[Category: Protein:protein interaction]]		[[Category: Protein:protein interaction]]

OCA at 14:36, 20 October 2012

OCA — Sat, 20 Oct 2012 14:36:55 GMT

←Older revision		Revision as of 14:36, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3qhy]] is a 2 chain structure ~~of [[TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)]]~~ with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis] and [http://en.wikipedia.org/wiki/Streptomyces_exfoliatus Streptomyces exfoliatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QHY OCA].	+	[[3qhy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis] and [http://en.wikipedia.org/wiki/Streptomyces_exfoliatus Streptomyces exfoliatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QHY OCA].

	==See Also==		==See Also==

OCA at 15:42, 26 July 2012

OCA — Thu, 26 Jul 2012 15:42:28 GMT

←Older revision		Revision as of 15:42, 26 July 2012
Line 1:		Line 1:
	[[Image:3qhy.png\|left\|200px]]		[[Image:3qhy.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3qhy", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3qhy\| PDB=3qhy \| SCENE= }}		{{STRUCTURE_3qhy\| PDB=3qhy \| SCENE= }}

	===Structural, thermodynamic and kinetic analysis of the picomolar binding affinity interaction of the beta-lactamase inhibitor protein-II (BLIP-II) with class A beta-lactamases===		===Structural, thermodynamic and kinetic analysis of the picomolar binding affinity interaction of the beta-lactamase inhibitor protein-II (BLIP-II) with class A beta-lactamases===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21775426}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 21775426 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_21775426}}		{{ABSTRACT_PUBMED_21775426}}

Line 22:		Line 11:

	==See Also==		==See Also==
-	*[[TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)]]	+	*[[TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)\|TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)]]

	==Reference==		==Reference==

OCA at 07:43, 29 February 2012

OCA — Wed, 29 Feb 2012 07:43:44 GMT

←Older revision		Revision as of 07:43, 29 February 2012
Line 11:		Line 11:
	===Structural, thermodynamic and kinetic analysis of the picomolar binding affinity interaction of the beta-lactamase inhibitor protein-II (BLIP-II) with class A beta-lactamases===		===Structural, thermodynamic and kinetic analysis of the picomolar binding affinity interaction of the beta-lactamase inhibitor protein-II (BLIP-II) with class A beta-lactamases===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21775426}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21775426 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21775426}}

	==About this Structure==		==About this Structure==
-	[[3qhy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis] and [http://en.wikipedia.org/wiki/Streptomyces_exfoliatus Streptomyces exfoliatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QHY OCA].	+	[[3qhy]] is a 2 chain structure of [[TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)]] with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis] and [http://en.wikipedia.org/wiki/Streptomyces_exfoliatus Streptomyces exfoliatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QHY OCA].
		+
		+	==See Also==
		+	*[[TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP)]]
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021775426</ref><references group="xtra"/>
	[[Category: Bacillus anthracis]]		[[Category: Bacillus anthracis]]
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]

OCA at 06:14, 27 July 2011

OCA — Wed, 27 Jul 2011 06:14:41 GMT

←Older revision		Revision as of 06:14, 27 July 2011
Line 1:		Line 1:
-	[[Image:3qhy.~~jpg~~\|left\|200px]]	+	[[Image:3qhy.png\|left\|200px]]

	<!--		<!--

OCA at 06:56, 20 July 2011

OCA — Wed, 20 Jul 2011 06:56:05 GMT

←Older revision		Revision as of 06:56, 20 July 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3qhy.jpg\|left\|200px]]

-	The ~~entry 3qhy~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3qhy", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3qhy\| PDB=3qhy \| SCENE= }}

-	~~Authors: Brown~~, ~~N.G., Chow, D.C., Sankaran, B., Zwart, P., Prasad, B.V.V., Palzkill, T., Berkeley Structural Genomics Center~~ (~~BSGC~~)	+	===Structural, thermodynamic and kinetic analysis of the picomolar binding affinity interaction of the beta-lactamase inhibitor protein-II (BLIP-II) with class A beta-lactamases===

-	~~Description~~: Structural, ~~thermodynamic and kinetic analysis of the picomolar binding affinity interaction of the beta~~-lactamase ~~inhibitor protein~~-~~II (BLIP~~-~~II) with class A beta~~-~~lactamases~~	+
		+	==About this Structure==
		+	[[3qhy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis] and [http://en.wikipedia.org/wiki/Streptomyces_exfoliatus Streptomyces exfoliatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QHY OCA].
		+	[[Category: Bacillus anthracis]]
		+	[[Category: Beta-lactamase]]
		+	[[Category: Streptomyces exfoliatus]]
		+	[[Category: BSGC, Berkeley Structural Genomics Center.]]
		+	[[Category: Brown, N G.]]
		+	[[Category: Chow, D C.]]
		+	[[Category: Palzkill, T.]]
		+	[[Category: Prasad, B V.V.]]
		+	[[Category: Sankaran, B.]]
		+	[[Category: Zwart, P.]]
		+	[[Category: Beta-lactamase]]
		+	[[Category: Beta-propeller]]
		+	[[Category: Enyzme-inhibitor complex]]
		+	[[Category: Hydrolase-hydrolase inhibitor complex]]
		+	[[Category: Protein:protein interaction]]

OCA at 05:49, 9 February 2011

OCA — Wed, 09 Feb 2011 05:49:54 GMT

←Older revision		Revision as of 05:49, 9 February 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3qhy is ON HOLD	+	The entry 3qhy is ON HOLD until Paper Publication

	Authors: Brown, N.G., Chow, D.C., Sankaran, B., Zwart, P., Prasad, B.V.V., Palzkill, T., Berkeley Structural Genomics Center (BSGC)		Authors: Brown, N.G., Chow, D.C., Sankaran, B., Zwart, P., Prasad, B.V.V., Palzkill, T., Berkeley Structural Genomics Center (BSGC)

-	Description: ~~Stuctural~~, thermodynamic and kinetic analysis of the picomolar binding affinity interaction of the beta-lactamase inhibitor protein-II (BLIP-II) with class A -lactamases	+	Description: Structural, thermodynamic and kinetic analysis of the picomolar binding affinity interaction of the beta-lactamase inhibitor protein-II (BLIP-II) with class A beta-lactamases