3qkw - Revision history

OCA at 10:48, 21 February 2024

OCA — Wed, 21 Feb 2024 10:48:21 GMT

←Older revision		Revision as of 10:48, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='3qkw' size='340' side='right'caption='[[3qkw]], [[Resolution\|resolution]] 2.29Å' scene=''>		<StructureSection load='3qkw' size='340' side='right'caption='[[3qkw]], [[Resolution\|resolution]] 2.29Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3qkw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_15912 Atcc 15912~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QKW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QKW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3qkw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_parasanguinis Streptococcus parasanguinis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QKW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QKW FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=UDP:URIDINE~~-~~5-DIPHOSPHATE'>UDP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.287Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~nss ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=5~~&id=1318 ATCC 15912])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qkw OCA], [https://pdbe.org/3qkw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qkw RCSB], [https://www.ebi.ac.uk/pdbsum/3qkw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qkw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qkw OCA], [https://pdbe.org/3qkw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qkw RCSB], [https://www.ebi.ac.uk/pdbsum/3qkw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qkw ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/GTF3_STRPA GTF3_STRPA] Required for polymorphic O-glycosylation of the serine-rich repeat protein Fap1. Catalyzes the second step in glycosylation of the serine-rich repeat protein in this bacteria. Transfers glucose from UDP-glucose to the terminal GlcNAc moiety of 3-O-(N-acetyl-alpha-D-glucosaminyl)-L-seryl-[protein] which results from the first glycosylation step of Fap1; does not use other sugar nucleotides as substrates.<ref>PMID:20164186</ref> <ref>PMID:21653318</ref> <ref>PMID:25404702</ref>
-	~~Serine~~-rich repeat ~~glycoproteins (SRRPs) are a growing family of bacterial adhesins found in many streptococci and staphylococci; they play important roles in bacterial biofilm formation and pathogenesis~~. ~~Glycosylation of this family of adhesins is essential for their biogenesis. A glucosyltransferase (Gtf3) catalyzes~~ the second step of glycosylation of ~~a SRRP (Fap1) from an oral streptococcus, Streptococcus parasanguinis. Although Gtf3 homologs are highly conserved~~ in ~~SRRP-containing streptococci, they share minimal homology with functionally known glycosyltransferases~~. ~~We report here the 2.3 A crystal structure of Gtf3. The structural analysis indicates that Gtf3 forms a tetramer and shares significant structural homology with glycosyltransferases~~ from ~~GT4, GT5, and GT20 subfamilies. Combining crystal structural analysis with site-directed mutagenesis and in vitro glycosyltransferase assays, we identified residues that are required for UDP or~~ UDP-~~Glucose binding and for oligomerization of Gtf3, and determined their contribution~~ to the ~~enzymatic activity~~ of ~~Gtf3. Further in vivo studies revealed that~~ the ~~critical amino acid residues identified by the structural analysis are crucial for~~ Fap1 ~~glycosylation in S. parasanguinis in vivo. Moreover, Gtf3 homologs from~~ other ~~streptococci were able to rescue the gtf3 knockout mutant of S. parasanguinis in vivo and catalyze the~~ sugar ~~transfer to the modified SRRP substrate in vitro, demonstrating the importance and conservation of the Gtf3 homologs in glycosylation of SRRPs~~. ~~As the Gtf3 homologs only exist in SRRP-containing streptococci, we conclude that the Gtf3 homologs represent a unique subfamily of glycosyltransferases.~~	+
-		+
-	Structural and functional analysis of a new subfamily of glycosyltransferases required for glycosylation of serine-rich streptococcal adhesins.,Zhu F, Erlandsen H, Ding L, Li J, Huang Y, Zhou M, Liang X, Ma J, Wu H J Biol Chem. 2011 Jun 7. PMID:21653318<ref>PMID:~~21653318~~</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.~~<br>	+
-	</~~div~~>	+
-	<~~div class="pdbe-citations 3qkw" style="background-color~~:~~#fffaf0;">~~</~~div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 15912]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ding, L]]	+	[[Category: Streptococcus parasanguinis]]
-	[[Category: Erlandsen, H]]	+	[[Category: Ding L]]
-	[[Category: Huang, Y]]	+	[[Category: Erlandsen H]]
-	[[Category: Liang, X]]	+	[[Category: Huang Y]]
-	[[Category: Ma, J B]]	+	[[Category: Liang X]]
-	[[Category: Wu, H]]	+	[[Category: Ma J-B]]
-	[[Category: Zhou, M]]	+	[[Category: Wu H]]
-	[[Category: Zhu, F]]	+	[[Category: Zhou M]]
-	~~[[Category: Glycosyltransferase]]~~	+	[[Category: Zhu F]]
-	~~[[Category: Gt-b fold]]~~	+
-	~~[[Category: Nucleotide sugar synthetase-like protein]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 06:13, 8 June 2022

OCA — Wed, 08 Jun 2022 06:13:13 GMT

←Older revision		Revision as of 06:13, 8 June 2022
Line 1:		Line 1:

	==Structure of Streptococcus parasangunini Gtf3 glycosyltransferase==		==Structure of Streptococcus parasangunini Gtf3 glycosyltransferase==
-	<StructureSection load='3qkw' size='340' side='right' caption='[[3qkw]], [[Resolution\|resolution]] 2.29Å' scene=''>	+	<StructureSection load='3qkw' size='340' side='right'caption='[[3qkw]], [[Resolution\|resolution]] 2.29Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3qkw]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_15912 Atcc 15912]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QKW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3QKW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3qkw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_15912 Atcc 15912]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QKW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QKW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nss ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1318 ATCC 15912])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nss ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1318 ATCC 15912])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3qkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qkw OCA], [~~http~~://pdbe.org/3qkw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3qkw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3qkw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3qkw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qkw OCA], [https://pdbe.org/3qkw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qkw RCSB], [https://www.ebi.ac.uk/pdbsum/3qkw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qkw ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 15912]]		[[Category: Atcc 15912]]
		+	[[Category: Large Structures]]
	[[Category: Ding, L]]		[[Category: Ding, L]]
	[[Category: Erlandsen, H]]		[[Category: Erlandsen, H]]

OCA at 17:56, 5 August 2016

OCA — Fri, 05 Aug 2016 17:56:49 GMT

←Older revision		Revision as of 17:56, 5 August 2016
Line 1:		Line 1:
		+
	==Structure of Streptococcus parasangunini Gtf3 glycosyltransferase==		==Structure of Streptococcus parasangunini Gtf3 glycosyltransferase==
	<StructureSection load='3qkw' size='340' side='right' caption='[[3qkw]], [[Resolution\|resolution]] 2.29Å' scene=''>		<StructureSection load='3qkw' size='340' side='right' caption='[[3qkw]], [[Resolution\|resolution]] 2.29Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3qkw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptococcus_parasanguinis Streptococcus parasanguinis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QKW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QKW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3qkw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_15912 Atcc 15912]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QKW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QKW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nss ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1318 ~~Streptococcus parasanguinis~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nss ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1318 ATCC 15912])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qkw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qkw RCSB], [http://www.ebi.ac.uk/pdbsum/3qkw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qkw OCA], [http://pdbe.org/3qkw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3qkw RCSB], [http://www.ebi.ac.uk/pdbsum/3qkw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3qkw ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3qkw" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Streptococcus parasanguinis~~]]	+	[[Category: Atcc 15912]]
	[[Category: Ding, L]]		[[Category: Ding, L]]
	[[Category: Erlandsen, H]]		[[Category: Erlandsen, H]]

OCA at 23:17, 3 January 2015

OCA — Sat, 03 Jan 2015 23:17:04 GMT

←Older revision		Revision as of 23:17, 3 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3qkw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_parasanguinis Streptococcus parasanguinis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QKW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QKW FirstGlance]. <br>		<table><tr><td colspan='2'>[[3qkw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_parasanguinis Streptococcus parasanguinis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QKW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QKW FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nss ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1318 Streptococcus parasanguinis])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nss ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1318 Streptococcus parasanguinis])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qkw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qkw RCSB], [http://www.ebi.ac.uk/pdbsum/3qkw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qkw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qkw RCSB], [http://www.ebi.ac.uk/pdbsum/3qkw PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 13:		Line 13:
	Structural and functional analysis of a new subfamily of glycosyltransferases required for glycosylation of serine-rich streptococcal adhesins.,Zhu F, Erlandsen H, Ding L, Li J, Huang Y, Zhou M, Liang X, Ma J, Wu H J Biol Chem. 2011 Jun 7. PMID:21653318<ref>PMID:21653318</ref>		Structural and functional analysis of a new subfamily of glycosyltransferases required for glycosylation of serine-rich streptococcal adhesins.,Zhu F, Erlandsen H, Ding L, Li J, Huang Y, Zhou M, Liang X, Ma J, Wu H J Biol Chem. 2011 Jun 7. PMID:21653318<ref>PMID:21653318</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 20:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Streptococcus parasanguinis]]		[[Category: Streptococcus parasanguinis]]
-	[[Category: Ding, L.]]	+	[[Category: Ding, L]]
-	[[Category: Erlandsen, H.]]	+	[[Category: Erlandsen, H]]
-	[[Category: Huang, Y.]]	+	[[Category: Huang, Y]]
-	[[Category: Liang, X.]]	+	[[Category: Liang, X]]
-	[[Category: Ma, J B.]]	+	[[Category: Ma, J B]]
-	[[Category: Wu, H.]]	+	[[Category: Wu, H]]
-	[[Category: Zhou, M.]]	+	[[Category: Zhou, M]]
-	[[Category: Zhu, F.]]	+	[[Category: Zhu, F]]
	[[Category: Glycosyltransferase]]		[[Category: Glycosyltransferase]]
	[[Category: Gt-b fold]]		[[Category: Gt-b fold]]
	[[Category: Nucleotide sugar synthetase-like protein]]		[[Category: Nucleotide sugar synthetase-like protein]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 05:29, 4 June 2014

OCA — Wed, 04 Jun 2014 05:29:56 GMT

←Older revision		Revision as of 05:29, 4 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3qkw.~~png~~\|~~left~~\|~~200px~~]]	+	==Structure of Streptococcus parasangunini Gtf3 glycosyltransferase==
		+	<StructureSection load='3qkw' size='340' side='right' caption='[[3qkw]], [[Resolution\|resolution]] 2.29Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3qkw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_parasanguinis Streptococcus parasanguinis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QKW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QKW FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nss ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1318 Streptococcus parasanguinis])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qkw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qkw RCSB], [http://www.ebi.ac.uk/pdbsum/3qkw PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Serine-rich repeat glycoproteins (SRRPs) are a growing family of bacterial adhesins found in many streptococci and staphylococci; they play important roles in bacterial biofilm formation and pathogenesis. Glycosylation of this family of adhesins is essential for their biogenesis. A glucosyltransferase (Gtf3) catalyzes the second step of glycosylation of a SRRP (Fap1) from an oral streptococcus, Streptococcus parasanguinis. Although Gtf3 homologs are highly conserved in SRRP-containing streptococci, they share minimal homology with functionally known glycosyltransferases. We report here the 2.3 A crystal structure of Gtf3. The structural analysis indicates that Gtf3 forms a tetramer and shares significant structural homology with glycosyltransferases from GT4, GT5, and GT20 subfamilies. Combining crystal structural analysis with site-directed mutagenesis and in vitro glycosyltransferase assays, we identified residues that are required for UDP or UDP-Glucose binding and for oligomerization of Gtf3, and determined their contribution to the enzymatic activity of Gtf3. Further in vivo studies revealed that the critical amino acid residues identified by the structural analysis are crucial for Fap1 glycosylation in S. parasanguinis in vivo. Moreover, Gtf3 homologs from other streptococci were able to rescue the gtf3 knockout mutant of S. parasanguinis in vivo and catalyze the sugar transfer to the modified SRRP substrate in vitro, demonstrating the importance and conservation of the Gtf3 homologs in glycosylation of SRRPs. As the Gtf3 homologs only exist in SRRP-containing streptococci, we conclude that the Gtf3 homologs represent a unique subfamily of glycosyltransferases.

-	<!--	+	Structural and functional analysis of a new subfamily of glycosyltransferases required for glycosylation of serine-rich streptococcal adhesins.,Zhu F, Erlandsen H, Ding L, Li J, Huang Y, Zhou M, Liang X, Ma J, Wu H J Biol Chem. 2011 Jun 7. PMID:21653318<ref>PMID:21653318</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3qkw"~~, ~~creates the "Structure Box" on the page.~~	+
-	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded)~~,	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3qkw\| PDB=3qkw \| SCENE= }}~~	+

-	~~===Structure~~ of ~~Streptococcus parasangunini Gtf3 glycosyltransferase===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_21653318}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 21653318 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_21653318}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3qkw]] is a 4 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Streptococcus_parasanguinis Streptococcus parasanguinis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QKW OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:021653318<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Streptococcus parasanguinis]]		[[Category: Streptococcus parasanguinis]]
	[[Category: Ding, L.]]		[[Category: Ding, L.]]

OCA at 05:06, 10 August 2011

OCA — Wed, 10 Aug 2011 05:06:30 GMT

←Older revision		Revision as of 05:06, 10 August 2011
Line 1:		Line 1:
-	[[Image:3qkw.~~jpg~~\|left\|200px]]	+	[[Image:3qkw.png\|left\|200px]]

	<!--		<!--

OCA at 15:54, 22 June 2011

OCA — Wed, 22 Jun 2011 15:54:50 GMT

←Older revision		Revision as of 15:54, 22 June 2011
Line 11:		Line 11:
	===Structure of Streptococcus parasangunini Gtf3 glycosyltransferase===		===Structure of Streptococcus parasangunini Gtf3 glycosyltransferase===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21653318}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21653318 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21653318}}

	==About this Structure==		==About this Structure==
	[[3qkw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_parasanguinis Streptococcus parasanguinis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QKW OCA].		[[3qkw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_parasanguinis Streptococcus parasanguinis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QKW OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021653318</ref><references group="xtra"/>
	[[Category: Streptococcus parasanguinis]]		[[Category: Streptococcus parasanguinis]]
	[[Category: Ding, L.]]		[[Category: Ding, L.]]
Line 23:		Line 32:
	[[Category: Zhou, M.]]		[[Category: Zhou, M.]]
	[[Category: Zhu, F.]]		[[Category: Zhu, F.]]
		+	[[Category: Glycosyltransferase]]
		+	[[Category: Gt-b fold]]
		+	[[Category: Nucleotide sugar synthetase-like protein]]
		+	[[Category: Transferase]]

OCA at 05:22, 8 June 2011

OCA — Wed, 08 Jun 2011 05:22:24 GMT

←Older revision		Revision as of 05:22, 8 June 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3qkw.jpg\|left\|200px]]

-	The ~~entry 3qkw~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3qkw", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3qkw\| PDB=3qkw \| SCENE= }}

-	~~Authors: Zhu, F., Erlandsen, H., Huang, Y., Ding, L., Zhou, M., Liang, X., Ma, J.-B., Wu, H.~~	+	===Structure of Streptococcus parasangunini Gtf3 glycosyltransferase===

-	~~Description:~~ Structure of Streptococcus ~~parasangunini Gtf3 glycosyltransferase~~	+
		+	==About this Structure==
		+	[[3qkw]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_parasanguinis Streptococcus parasanguinis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QKW OCA].
		+	[[Category: Streptococcus parasanguinis]]
		+	[[Category: Ding, L.]]
		+	[[Category: Erlandsen, H.]]
		+	[[Category: Huang, Y.]]
		+	[[Category: Liang, X.]]
		+	[[Category: Ma, J B.]]
		+	[[Category: Wu, H.]]
		+	[[Category: Zhou, M.]]
		+	[[Category: Zhu, F.]]

OCA: Protected "3qkw" [edit=sysop:move=sysop]

OCA — Wed, 09 Feb 2011 05:51:21 GMT

Protected "3qkw" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:51, 9 February 2011

OCA: New page: '''Unreleased structure''' The entry 3qkw is ON HOLD Authors: Zhu, F., Erlandsen, H., Huang, Y., Ding, L., Zhou, M., Liang, X., Ma, J.-B., Wu, H. Description: Structure of Streptococcu...

OCA — Wed, 09 Feb 2011 05:51:21 GMT

New page: '''Unreleased structure''' The entry 3qkw is ON HOLD Authors: Zhu, F., Erlandsen, H., Huang, Y., Ding, L., Zhou, M., Liang, X., Ma, J.-B., Wu, H. Description: Structure of Streptococcu...

New page

'''Unreleased structure'''

The entry 3qkw is ON HOLD

Authors: Zhu, F., Erlandsen, H., Huang, Y., Ding, L., Zhou, M., Liang, X., Ma, J.-B., Wu, H.

Description: Structure of Streptococcus parasangunini Gtf3 glycosyltransferase