3qrc - Revision history

OCA at 11:55, 14 March 2024

OCA — Thu, 14 Mar 2024 11:55:44 GMT

←Older revision		Revision as of 11:55, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3qrc' size='340' side='right'caption='[[3qrc]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='3qrc' size='340' side='right'caption='[[3qrc]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3qrc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_pestis"_(lehmann_and_neumann_1896)_migula_1900 "bacillus~~ pestis~~" (lehmann and neumann 1896) migula 1900~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QRC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QRC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3qrc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QRC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QRC FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>~~, ~~<scene name='pdbligand=GU4:2,3,4,6-TETRA-O-SULFONATO-ALPHA-D-GLUCOPYRANOSE'>GU4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.852Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~YYJ~~:'>~~YYJ~~</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>, <scene name='pdbligand=GU4:2,3,4,6-TETRA-O-SULFONATO-ALPHA-D-GLUCOPYRANOSE'>GU4</scene>, <scene name='pdbligand=PRD_900013:sucrose+octasulfate'>PRD_900013</scene>, <scene name='pdbligand=YYJ:1,3,4,6-tetra-O-sulfo-beta-D-fructofuranose'>YYJ</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b~~></td><~~td class~~=~~"sblockDat"><div style~~=~~'overflow~~: ~~auto; max-height: 3em;~~'>~~[[3qra\|3qra]]~~</~~div~~><~~/td></tr>~~	+
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">ail, YPO2905 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=632 "Bacillus pestis" (Lehmann and Neumann 1896) Migula 1900])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qrc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qrc OCA], [https://pdbe.org/3qrc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qrc RCSB], [https://www.ebi.ac.uk/pdbsum/3qrc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qrc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qrc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qrc OCA], [https://pdbe.org/3qrc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qrc RCSB], [https://www.ebi.ac.uk/pdbsum/3qrc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qrc ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q8D0Z7_YERPE Q8D0Z7_YERPE]
-	Ail is an outer membrane protein from Yersinia pestis that is highly expressed in a rodent model of bubonic plague, making it a good candidate for vaccine development. Ail is important for attaching to host cells and evading host immune responses, facilitating rapid progression of a plague infection. Binding to host cells is important for injection of cytotoxic Yersinia outer proteins. To learn more about how Ail mediates adhesion, we solved two high-resolution crystal structures of Ail, with no ligand bound and in complex with a heparin analog called sucrose octasulfate. We identified multiple adhesion targets, including laminin and heparin, and showed that a 40 kDa domain of laminin called LG4-5 specifically binds to Ail. We also evaluated the contribution of laminin to delivery of Yops to HEp-2 cells. This work constitutes a structural description of how a bacterial outer membrane protein uses a multivalent approach to bind host cells.	+
-		+
-	~~Structural Insights into Ail-Mediated Adhesion in Yersinia pestis.,Yamashita S, Lukacik P, Barnard TJ, Noinaj N, Felek S, Tsang TM, Krukonis ES, Hinnebusch BJ, Buchanan SK Structure. 2011 Nov 9;19(11)~~:~~1672-82. PMID:22078566<ref>PMID:22078566<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3qrc" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Buchanan~~, S K~~]]	+	[[Category: Yersinia pestis]]
-	[[Category: Lukacik, P]]	+	[[Category: Buchanan SK]]
-	[[Category: Noinaj, N]]	+	[[Category: Lukacik P]]
-	[[Category: Yamashita, S]]	+	[[Category: Noinaj N]]
-	~~[[Category: Attachment and invasion virulence]]~~	+	[[Category: Yamashita S]]
-	~~[[Category: Beta-barrel protein]]~~	+
-	~~[[Category: Cell invasion]]~~	+
-	~~[[Category: Heparin]]~~	+
-	~~[[Category: Laminin~~]]	+

OCA at 06:20, 8 June 2022

OCA — Wed, 08 Jun 2022 06:20:46 GMT

←Older revision		Revision as of 06:20, 8 June 2022
Line 1:		Line 1:

	==The crystal structure of Ail, the attachment invasion locus protein of Yersinia pestis, in complex with the heparin analogue sucrose octasulfate==		==The crystal structure of Ail, the attachment invasion locus protein of Yersinia pestis, in complex with the heparin analogue sucrose octasulfate==
-	<StructureSection load='3qrc' size='340' side='right' caption='[[3qrc]], [[Resolution\|resolution]] 1.85Å' scene=''>	+	<StructureSection load='3qrc' size='340' side='right'caption='[[3qrc]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3qrc]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_pestis"_(lehmann_and_neumann_1896)_migula_1900 "bacillus pestis" (lehmann and neumann 1896) migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QRC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3QRC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3qrc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_pestis"_(lehmann_and_neumann_1896)_migula_1900 "bacillus pestis" (lehmann and neumann 1896) migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QRC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QRC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>, <scene name='pdbligand=~~SCR~~:~~SUCROSE+OCTASULFATE~~'>~~SCR~~</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>, <scene name='pdbligand=GU4:2,3,4,6-TETRA-O-SULFONATO-ALPHA-D-GLUCOPYRANOSE'>GU4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qra\|3qra]]</td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=YYJ:'>YYJ</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ail, YPO2905 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=632 "Bacillus pestis" (Lehmann and Neumann 1896) Migula 1900])</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3qra\|3qra]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3qrc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qrc OCA], [~~http~~://pdbe.org/3qrc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3qrc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3qrc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3qrc ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ail, YPO2905 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=632 "Bacillus pestis" (Lehmann and Neumann 1896) Migula 1900])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qrc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qrc OCA], [https://pdbe.org/3qrc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qrc RCSB], [https://www.ebi.ac.uk/pdbsum/3qrc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qrc ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Buchanan, S K]]		[[Category: Buchanan, S K]]
	[[Category: Lukacik, P]]		[[Category: Lukacik, P]]

OCA at 19:54, 4 August 2016

OCA — Thu, 04 Aug 2016 19:54:15 GMT

←Older revision		Revision as of 19:54, 4 August 2016
Line 1:		Line 1:
		+
	==The crystal structure of Ail, the attachment invasion locus protein of Yersinia pestis, in complex with the heparin analogue sucrose octasulfate==		==The crystal structure of Ail, the attachment invasion locus protein of Yersinia pestis, in complex with the heparin analogue sucrose octasulfate==
	<StructureSection load='3qrc' size='340' side='right' caption='[[3qrc]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='3qrc' size='340' side='right' caption='[[3qrc]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3qrc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Yersinia_pestis Yersinia~~ pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QRC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QRC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3qrc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pestis"_(lehmann_and_neumann_1896)_migula_1900 "bacillus pestis" (lehmann and neumann 1896) migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QRC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QRC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>, <scene name='pdbligand=SCR:SUCROSE+OCTASULFATE'>SCR</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>, <scene name='pdbligand=SCR:SUCROSE+OCTASULFATE'>SCR</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qra\|3qra]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qra\|3qra]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ail, YPO2905 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=632 ~~Yersinia~~ pestis])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ail, YPO2905 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=632 "Bacillus pestis" (Lehmann and Neumann 1896) Migula 1900])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qrc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qrc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qrc RCSB], [http://www.ebi.ac.uk/pdbsum/3qrc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qrc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qrc OCA], [http://pdbe.org/3qrc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3qrc RCSB], [http://www.ebi.ac.uk/pdbsum/3qrc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3qrc ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3qrc" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Yersinia pestis]]
	[[Category: Buchanan, S K]]		[[Category: Buchanan, S K]]
	[[Category: Lukacik, P]]		[[Category: Lukacik, P]]

OCA at 22:08, 3 January 2015

OCA — Sat, 03 Jan 2015 22:08:19 GMT

←Older revision		Revision as of 22:08, 3 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3qrc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QRC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QRC FirstGlance]. <br>		<table><tr><td colspan='2'>[[3qrc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QRC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QRC FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>, <scene name='pdbligand=SCR:SUCROSE+OCTASULFATE'>SCR</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>, <scene name='pdbligand=SCR:SUCROSE+OCTASULFATE'>SCR</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qra\|3qra]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qra\|3qra]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ail, YPO2905 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=632 Yersinia pestis])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ail, YPO2905 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=632 Yersinia pestis])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qrc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qrc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qrc RCSB], [http://www.ebi.ac.uk/pdbsum/3qrc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qrc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qrc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qrc RCSB], [http://www.ebi.ac.uk/pdbsum/3qrc PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 14:
	Structural Insights into Ail-Mediated Adhesion in Yersinia pestis.,Yamashita S, Lukacik P, Barnard TJ, Noinaj N, Felek S, Tsang TM, Krukonis ES, Hinnebusch BJ, Buchanan SK Structure. 2011 Nov 9;19(11):1672-82. PMID:22078566<ref>PMID:22078566</ref>		Structural Insights into Ail-Mediated Adhesion in Yersinia pestis.,Yamashita S, Lukacik P, Barnard TJ, Noinaj N, Felek S, Tsang TM, Krukonis ES, Hinnebusch BJ, Buchanan SK Structure. 2011 Nov 9;19(11):1672-82. PMID:22078566<ref>PMID:22078566</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Yersinia pestis]]		[[Category: Yersinia pestis]]
-	[[Category: Buchanan, S K.]]	+	[[Category: Buchanan, S K]]
-	[[Category: Lukacik, P.]]	+	[[Category: Lukacik, P]]
-	[[Category: Noinaj, N.]]	+	[[Category: Noinaj, N]]
-	[[Category: Yamashita, S.]]	+	[[Category: Yamashita, S]]
	[[Category: Attachment and invasion virulence]]		[[Category: Attachment and invasion virulence]]
	[[Category: Beta-barrel protein]]		[[Category: Beta-barrel protein]]

OCA at 05:31, 4 June 2014

OCA — Wed, 04 Jun 2014 05:31:01 GMT

←Older revision		Revision as of 05:31, 4 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3qrc.~~jpg~~\|~~left~~\|~~200px~~]]	+	==The crystal structure of Ail, the attachment invasion locus protein of Yersinia pestis, in complex with the heparin analogue sucrose octasulfate==
		+	<StructureSection load='3qrc' size='340' side='right' caption='[[3qrc]], [[Resolution\|resolution]] 1.85Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3qrc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QRC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QRC FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>, <scene name='pdbligand=SCR:SUCROSE+OCTASULFATE'>SCR</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qra\|3qra]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ail, YPO2905 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=632 Yersinia pestis])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qrc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qrc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qrc RCSB], [http://www.ebi.ac.uk/pdbsum/3qrc PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ail is an outer membrane protein from Yersinia pestis that is highly expressed in a rodent model of bubonic plague, making it a good candidate for vaccine development. Ail is important for attaching to host cells and evading host immune responses, facilitating rapid progression of a plague infection. Binding to host cells is important for injection of cytotoxic Yersinia outer proteins. To learn more about how Ail mediates adhesion, we solved two high-resolution crystal structures of Ail, with no ligand bound and in complex with a heparin analog called sucrose octasulfate. We identified multiple adhesion targets, including laminin and heparin, and showed that a 40 kDa domain of laminin called LG4-5 specifically binds to Ail. We also evaluated the contribution of laminin to delivery of Yops to HEp-2 cells. This work constitutes a structural description of how a bacterial outer membrane protein uses a multivalent approach to bind host cells.

-	<!--	+	Structural Insights into Ail-Mediated Adhesion in Yersinia pestis.,Yamashita S, Lukacik P, Barnard TJ, Noinaj N, Felek S, Tsang TM, Krukonis ES, Hinnebusch BJ, Buchanan SK Structure. 2011 Nov 9;19(11):1672-82. PMID:22078566<ref>PMID:22078566</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3qrc"~~, ~~creates the "~~Structure ~~Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display.~~	+
-	-->	+
-	~~{{STRUCTURE_3qrc\| PDB=3qrc \| SCENE= }}~~	+

-	~~===The crystal structure of Ail~~, ~~the attachment invasion locus protein~~ of ~~Yersinia pestis, in complex with~~ the ~~heparin analogue sucrose octasulfate===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22078566}}, adds the Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22078566 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_22078566}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3qrc]] is a 2 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QRC OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022078566<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Yersinia pestis]]		[[Category: Yersinia pestis]]
	[[Category: Buchanan, S K.]]		[[Category: Buchanan, S K.]]

OCA at 19:03, 23 November 2011

OCA — Wed, 23 Nov 2011 19:03:29 GMT

←Older revision		Revision as of 19:03, 23 November 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3qrc.jpg\|left\|200px]]

-	The ~~entry 3qrc~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3qrc", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3qrc\| PDB=3qrc \| SCENE= }}

-	~~Authors: Yamashita~~, S., ~~Lukacik, P., Noinaj, N., Buchanan, S.K.~~	+	===The crystal structure of Ail, the attachment invasion locus protein of Yersinia pestis, in complex with the heparin analogue sucrose octasulfate===

-	~~Description:~~ The ~~crystal structure of Ail~~, the ~~attachment invasion locus protein of~~ Yersinia pestis, ~~in complex with the heparin analogue sucrose octasulfate~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22078566}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22078566 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22078566}}
		+
		+	==About this Structure==
		+	[[3qrc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QRC OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022078566</ref><references group="xtra"/>
		+	[[Category: Yersinia pestis]]
		+	[[Category: Buchanan, S K.]]
		+	[[Category: Lukacik, P.]]
		+	[[Category: Noinaj, N.]]
		+	[[Category: Yamashita, S.]]
		+	[[Category: Attachment and invasion virulence]]
		+	[[Category: Beta-barrel protein]]
		+	[[Category: Cell invasion]]
		+	[[Category: Heparin]]
		+	[[Category: Laminin]]

OCA at 06:06, 9 March 2011

OCA — Wed, 09 Mar 2011 06:06:25 GMT

←Older revision		Revision as of 06:06, 9 March 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3qrc is ON HOLD	+	The entry 3qrc is ON HOLD until Paper Publication

	Authors: Yamashita, S., Lukacik, P., Noinaj, N., Buchanan, S.K.		Authors: Yamashita, S., Lukacik, P., Noinaj, N., Buchanan, S.K.

	Description: The crystal structure of Ail, the attachment invasion locus protein of Yersinia pestis, in complex with the heparin analogue sucrose octasulfate		Description: The crystal structure of Ail, the attachment invasion locus protein of Yersinia pestis, in complex with the heparin analogue sucrose octasulfate

OCA at 06:42, 2 March 2011

OCA — Wed, 02 Mar 2011 06:42:05 GMT

←Older revision		Revision as of 06:42, 2 March 2011
Line 3:		Line 3:
	The entry 3qrc is ON HOLD		The entry 3qrc is ON HOLD

-	Authors: Yamashita, S, Lukacik, P, Noinaj, N, Buchanan, SK	+	Authors: Yamashita, S., Lukacik, P., Noinaj, N., Buchanan, S.K.

	Description: The crystal structure of Ail, the attachment invasion locus protein of Yersinia pestis, in complex with the heparin analogue sucrose octasulfate		Description: The crystal structure of Ail, the attachment invasion locus protein of Yersinia pestis, in complex with the heparin analogue sucrose octasulfate

OCA: Protected "3qrc" [edit=sysop:move=sysop]

OCA — Wed, 23 Feb 2011 09:41:28 GMT

Protected "3qrc" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:41, 23 February 2011

OCA: New page: '''Unreleased structure''' The entry 3qrc is ON HOLD Authors: Yamashita, S, Lukacik, P, Noinaj, N, Buchanan, SK Description: The crystal structure of Ail, the attachment invasion locus...

OCA — Wed, 23 Feb 2011 09:41:27 GMT

New page: '''Unreleased structure''' The entry 3qrc is ON HOLD Authors: Yamashita, S, Lukacik, P, Noinaj, N, Buchanan, SK Description: The crystal structure of Ail, the attachment invasion locus...

New page

'''Unreleased structure'''

The entry 3qrc is ON HOLD

Authors: Yamashita, S, Lukacik, P, Noinaj, N, Buchanan, SK

Description: The crystal structure of Ail, the attachment invasion locus protein of Yersinia pestis, in complex with the heparin analogue sucrose octasulfate