3qvv - Revision history

OCA at 12:00, 14 March 2024

OCA — Thu, 14 Mar 2024 12:00:37 GMT

←Older revision		Revision as of 12:00, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3qvv' size='340' side='right'caption='[[3qvv]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='3qvv' size='340' side='right'caption='[[3qvv]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3qvv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QVV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QVV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3qvv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QVV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QVV FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=3QV:7~~-~~HYDROXY-2-OXO-2H-CHROMENE-3-CARBONITRILE'>3QV</scene>~~, ~~<scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.35Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[3qvu\|3qvu]], [[1ls6\|1ls6]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3QV:7-HYDROXY-2-OXO-2H-CHROMENE-3-CARBONITRILE'>3QV</scene>, <scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>SULT1A1, STP, STP1, OK/SW-cl.88 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~9606 HUMAN])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Aryl_sulfotransferase Aryl sulfotransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.1 2.8.2.1]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qvv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qvv OCA], [https://pdbe.org/3qvv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qvv RCSB], [https://www.ebi.ac.uk/pdbsum/3qvv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qvv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qvv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qvv OCA], [https://pdbe.org/3qvv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qvv RCSB], [https://www.ebi.ac.uk/pdbsum/3qvv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qvv ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ST1A1_HUMAN ST1A1_HUMAN]] Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of catecholamines, phenolic drugs and neurotransmitters. Has also estrogen sulfotransferase activity. responsible for the sulfonation and activation of minoxidil. Is Mediates the metabolic activation of carcinogenic N-hydroxyarylamines to DNA binding products and could so participate as modulating factor of cancer risk.<ref>PMID:12471039</ref> <ref>PMID:16221673</ref>	+	[https://www.uniprot.org/uniprot/ST1A1_HUMAN ST1A1_HUMAN] Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of catecholamines, phenolic drugs and neurotransmitters. Has also estrogen sulfotransferase activity. responsible for the sulfonation and activation of minoxidil. Is Mediates the metabolic activation of carcinogenic N-hydroxyarylamines to DNA binding products and could so participate as modulating factor of cancer risk.<ref>PMID:12471039</ref> <ref>PMID:16221673</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Large libraries of randomly mutated genes are applied in directed evolution experiments in order to obtain sufficient variability. These libraries, however, contain mostly inactive variants, and the very low frequency of improved variants can only be isolated by high-throughput screening. Small but efficient libraries comprise an attractive alternative. Here, we describe the application of ancestral libraries-libraries based on mutations predicted by phylogenetic analysis and ancestral inference. We designed and constructed such libraries using serum paraoxonases and cytosolic sulfotransferases (SULTs) as model enzymes. Both of these enzyme families exhibit a range of activities in drug metabolism and detoxification of xenobiotics. The ancestral serum paraoxonase and SULT libraries were screened by low-throughput means, including HPLC, using substrates and/or reactions with which all family members exhibit low activity. The libraries showed a remarkably high frequency of highly polymorphic and functionally diverse variants. Screening of as few as 300 variants enabled the isolation of mutants with up to 50-fold higher activity than the starting point enzyme. Structural and kinetic characterizations of an evolved SULT variant show how few ancestral mutations reshaped the active site and modulated the enzyme's specificity. Ancestral libraries therefore comprise a means of focusing diversity to positions and mutations that readily trigger changes in substrate and/or reaction specificity, thereby facilitating the isolation of new enzyme variants for a variety of different substrates and reactions by medium-throughput or even low-throughput screens.	+
-		+
-	Directed evolution of sulfotransferases and paraoxonases by ancestral libraries.,Alcolombri U, Elias M, Tawfik DS J Mol Biol. 2011 Aug 26;411(4):837-53. Epub 2011 Jun 24. PMID:21723874<ref>PMID:21723874</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3qvv" style="background-color:#fffaf0;"></div~~>	+

	==See Also==		==See Also==
Line 28:		Line 17:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aryl sulfotransferase]]~~	+	[[Category: Homo sapiens]]
-	~~[[Category: Human~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Alkolombri, U]]	+	[[Category: Alkolombri U]]
-	[[Category: Elias, M]]	+	[[Category: Elias M]]
-	[[Category: Tawfik~~, D S]]~~	+	[[Category: Tawfik DS]]
-	~~[[Category: Transferase~~]]	+

OCA at 05:38, 15 June 2022

OCA — Wed, 15 Jun 2022 05:38:31 GMT

←Older revision		Revision as of 05:38, 15 June 2022
Line 1:		Line 1:

	==Crystal structure of Ancestral variant b9 of SULT 1A1 in complex with PAP and 3-CyC==		==Crystal structure of Ancestral variant b9 of SULT 1A1 in complex with PAP and 3-CyC==
-	<StructureSection load='3qvv' size='340' side='right' caption='[[3qvv]], [[Resolution\|resolution]] 2.35Å' scene=''>	+	<StructureSection load='3qvv' size='340' side='right'caption='[[3qvv]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3qvv]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QVV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3QVV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3qvv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QVV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QVV FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3QV:7-HYDROXY-2-OXO-2H-CHROMENE-3-CARBONITRILE'>3QV</scene>, <scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3QV:7-HYDROXY-2-OXO-2H-CHROMENE-3-CARBONITRILE'>3QV</scene>, <scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qvu\|3qvu]], [[1ls6\|1ls6]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3qvu\|3qvu]], [[1ls6\|1ls6]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SULT1A1, STP, STP1, OK/SW-cl.88 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SULT1A1, STP, STP1, OK/SW-cl.88 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Aryl_sulfotransferase Aryl sulfotransferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.1 2.8.2.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Aryl_sulfotransferase Aryl sulfotransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.1 2.8.2.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3qvv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qvv OCA], [~~http~~://pdbe.org/3qvv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3qvv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3qvv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3qvv ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qvv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qvv OCA], [https://pdbe.org/3qvv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qvv RCSB], [https://www.ebi.ac.uk/pdbsum/3qvv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qvv ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ST1A1_HUMAN ST1A1_HUMAN]] Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of catecholamines, phenolic drugs and neurotransmitters. Has also estrogen sulfotransferase activity. responsible for the sulfonation and activation of minoxidil. Is Mediates the metabolic activation of carcinogenic N-hydroxyarylamines to DNA binding products and could so participate as modulating factor of cancer risk.<ref>PMID:12471039</ref> <ref>PMID:16221673</ref>	+	[[https://www.uniprot.org/uniprot/ST1A1_HUMAN ST1A1_HUMAN]] Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of catecholamines, phenolic drugs and neurotransmitters. Has also estrogen sulfotransferase activity. responsible for the sulfonation and activation of minoxidil. Is Mediates the metabolic activation of carcinogenic N-hydroxyarylamines to DNA binding products and could so participate as modulating factor of cancer risk.<ref>PMID:12471039</ref> <ref>PMID:16221673</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:

	==See Also==		==See Also==
-	*[[Sulfotransferase\|Sulfotransferase]]	+	*[[Sulfotransferase 3D structures\|Sulfotransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 30:		Line 30:
	[[Category: Aryl sulfotransferase]]		[[Category: Aryl sulfotransferase]]
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Alkolombri, U]]		[[Category: Alkolombri, U]]
	[[Category: Elias, M]]		[[Category: Elias, M]]
	[[Category: Tawfik, D S]]		[[Category: Tawfik, D S]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 03:24, 5 August 2016

OCA — Fri, 05 Aug 2016 03:24:43 GMT

←Older revision		Revision as of 03:24, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Ancestral variant b9 of SULT 1A1 in complex with PAP and 3-CyC==		==Crystal structure of Ancestral variant b9 of SULT 1A1 in complex with PAP and 3-CyC==
	<StructureSection load='3qvv' size='340' side='right' caption='[[3qvv]], [[Resolution\|resolution]] 2.35Å' scene=''>		<StructureSection load='3qvv' size='340' side='right' caption='[[3qvv]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3qvv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QVV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QVV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3qvv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QVV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QVV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3QV:7-HYDROXY-2-OXO-2H-CHROMENE-3-CARBONITRILE'>3QV</scene>, <scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3QV:7-HYDROXY-2-OXO-2H-CHROMENE-3-CARBONITRILE'>3QV</scene>, <scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qvu\|3qvu]], [[1ls6\|1ls6]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qvu\|3qvu]], [[1ls6\|1ls6]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SULT1A1, STP, STP1, OK/SW-cl.88 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SULT1A1, STP, STP1, OK/SW-cl.88 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aryl_sulfotransferase Aryl sulfotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.1 2.8.2.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aryl_sulfotransferase Aryl sulfotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.1 2.8.2.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qvv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qvv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qvv RCSB], [http://www.ebi.ac.uk/pdbsum/3qvv PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qvv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qvv OCA], [http://pdbe.org/3qvv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3qvv RCSB], [http://www.ebi.ac.uk/pdbsum/3qvv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3qvv ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3qvv" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 27:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Aryl sulfotransferase]]		[[Category: Aryl sulfotransferase]]
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Alkolombri, U]]		[[Category: Alkolombri, U]]
	[[Category: Elias, M]]		[[Category: Elias, M]]
	[[Category: Tawfik, D S]]		[[Category: Tawfik, D S]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 22:29, 24 December 2014

OCA — Wed, 24 Dec 2014 22:29:20 GMT

←Older revision		Revision as of 22:29, 24 December 2014
Line 9:		Line 9:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qvv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qvv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qvv RCSB], [http://www.ebi.ac.uk/pdbsum/3qvv PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qvv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qvv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qvv RCSB], [http://www.ebi.ac.uk/pdbsum/3qvv PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ST1A1_HUMAN ST1A1_HUMAN]] Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of catecholamines, phenolic drugs and neurotransmitters. Has also estrogen sulfotransferase activity. responsible for the sulfonation and activation of minoxidil. Is Mediates the metabolic activation of carcinogenic N-hydroxyarylamines to DNA binding products and could so participate as modulating factor of cancer risk.<ref>PMID:12471039</ref> <ref>PMID:16221673</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 11:50, 9 December 2014

OCA — Tue, 09 Dec 2014 11:50:45 GMT

←Older revision		Revision as of 11:50, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3qvv.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of Ancestral variant b9 of SULT 1A1 in complex with PAP and 3-CyC==
		+	<StructureSection load='3qvv' size='340' side='right' caption='[[3qvv]], [[Resolution\|resolution]] 2.35Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3qvv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QVV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QVV FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3QV:7-HYDROXY-2-OXO-2H-CHROMENE-3-CARBONITRILE'>3QV</scene>, <scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3qvu\|3qvu]], [[1ls6\|1ls6]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SULT1A1, STP, STP1, OK/SW-cl.88 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aryl_sulfotransferase Aryl sulfotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.1 2.8.2.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qvv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qvv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qvv RCSB], [http://www.ebi.ac.uk/pdbsum/3qvv PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Large libraries of randomly mutated genes are applied in directed evolution experiments in order to obtain sufficient variability. These libraries, however, contain mostly inactive variants, and the very low frequency of improved variants can only be isolated by high-throughput screening. Small but efficient libraries comprise an attractive alternative. Here, we describe the application of ancestral libraries-libraries based on mutations predicted by phylogenetic analysis and ancestral inference. We designed and constructed such libraries using serum paraoxonases and cytosolic sulfotransferases (SULTs) as model enzymes. Both of these enzyme families exhibit a range of activities in drug metabolism and detoxification of xenobiotics. The ancestral serum paraoxonase and SULT libraries were screened by low-throughput means, including HPLC, using substrates and/or reactions with which all family members exhibit low activity. The libraries showed a remarkably high frequency of highly polymorphic and functionally diverse variants. Screening of as few as 300 variants enabled the isolation of mutants with up to 50-fold higher activity than the starting point enzyme. Structural and kinetic characterizations of an evolved SULT variant show how few ancestral mutations reshaped the active site and modulated the enzyme's specificity. Ancestral libraries therefore comprise a means of focusing diversity to positions and mutations that readily trigger changes in substrate and/or reaction specificity, thereby facilitating the isolation of new enzyme variants for a variety of different substrates and reactions by medium-throughput or even low-throughput screens.

-	~~{{STRUCTURE_3qvv\| PDB=3qvv \| SCENE= }}~~	+	Directed evolution of sulfotransferases and paraoxonases by ancestral libraries.,Alcolombri U, Elias M, Tawfik DS J Mol Biol. 2011 Aug 26;411(4):837-53. Epub 2011 Jun 24. PMID:21723874<ref>PMID:21723874</ref>

-	~~===Crystal structure~~ of ~~Ancestral variant b9 of SULT 1A1 in complex with PAP and 3-CyC===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_21723874}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3qvv]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3QVV OCA].~~	+

	==See Also==		==See Also==
	*[[Sulfotransferase\|Sulfotransferase]]		*[[Sulfotransferase\|Sulfotransferase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:021723874<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Aryl sulfotransferase]]		[[Category: Aryl sulfotransferase]]
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Alkolombri, U.]]	+	[[Category: Alkolombri, U]]
-	[[Category: Elias, M.]]	+	[[Category: Elias, M]]
-	[[Category: Tawfik, D S~~.]]~~	+	[[Category: Tawfik, D S]]
-	~~[[Category: Aryl sulfotransferase~~]]	+
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 09:46, 20 October 2012

OCA — Sat, 20 Oct 2012 09:46:38 GMT

←Older revision		Revision as of 09:46, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3qvv]] is a 2 chain structure ~~of [[Sulfotransferase]]~~ with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QVV OCA].	+	[[3qvv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QVV OCA].

	==See Also==		==See Also==

OCA at 23:54, 25 July 2012

OCA — Wed, 25 Jul 2012 23:54:49 GMT

←Older revision		Revision as of 23:54, 25 July 2012
Line 1:		Line 1:
-	[[Image:3qvv.~~jpg~~\|left\|200px]]	+	[[Image:3qvv.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3qvv", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3qvv\| PDB=3qvv \| SCENE= }}		{{STRUCTURE_3qvv\| PDB=3qvv \| SCENE= }}

	===Crystal structure of Ancestral variant b9 of SULT 1A1 in complex with PAP and 3-CyC===		===Crystal structure of Ancestral variant b9 of SULT 1A1 in complex with PAP and 3-CyC===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21723874}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 21723874 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_21723874}}		{{ABSTRACT_PUBMED_21723874}}

	==About this Structure==		==About this Structure==
-	[[3qvv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QVV OCA].	+	[[3qvv]] is a 2 chain structure of [[Sulfotransferase]] with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QVV OCA].
		+
		+	==See Also==
		+	*[[Sulfotransferase\|Sulfotransferase]]

	==Reference==		==Reference==

OCA at 06:48, 31 August 2011

OCA — Wed, 31 Aug 2011 06:48:57 GMT

←Older revision		Revision as of 06:48, 31 August 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3qvv.jpg\|left\|200px]]

-	The ~~entry 3qvv~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3qvv", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3qvv\| PDB=3qvv \| SCENE= }}

-	~~Authors: Alkolombri, U., Elias, M., Tawfik, D.S.~~	+	===Crystal structure of Ancestral variant b9 of SULT 1A1 in complex with PAP and 3-CyC===

-	~~Description~~: ~~Crystal~~ structure ~~of Ancestral variant b9 of SULT 1A1 in complex~~ with ~~PAP and 3~~-~~CyC~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21723874}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21723874 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21723874}}
		+
		+	==About this Structure==
		+	[[3qvv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QVV OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021723874</ref><references group="xtra"/>
		+	[[Category: Aryl sulfotransferase]]
		+	[[Category: Homo sapiens]]
		+	[[Category: Alkolombri, U.]]
		+	[[Category: Elias, M.]]
		+	[[Category: Tawfik, D S.]]
		+	[[Category: Aryl sulfotransferase]]
		+	[[Category: Transferase]]

OCA at 10:18, 21 April 2011

OCA — Thu, 21 Apr 2011 10:18:22 GMT

←Older revision		Revision as of 10:18, 21 April 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3qvv is ON HOLD	+	The entry 3qvv is ON HOLD until Paper Publication

-	Authors: ~~Alcolombri~~, U., Elias, M., Tawfik, D.S.	+	Authors: Alkolombri, U., Elias, M., Tawfik, D.S.

	Description: Crystal structure of Ancestral variant b9 of SULT 1A1 in complex with PAP and 3-CyC		Description: Crystal structure of Ancestral variant b9 of SULT 1A1 in complex with PAP and 3-CyC

OCA at 06:46, 23 March 2011

OCA — Wed, 23 Mar 2011 06:46:34 GMT

←Older revision		Revision as of 06:46, 23 March 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3qvv is ON HOLD ~~until Paper Publication~~	+	The entry 3qvv is ON HOLD

	Authors: Alcolombri, U., Elias, M., Tawfik, D.S.		Authors: Alcolombri, U., Elias, M., Tawfik, D.S.

	Description: Crystal structure of Ancestral variant b9 of SULT 1A1 in complex with PAP and 3-CyC		Description: Crystal structure of Ancestral variant b9 of SULT 1A1 in complex with PAP and 3-CyC