3qyq - Revision history

OCA at 12:03, 14 March 2024

OCA — Thu, 14 Mar 2024 12:03:17 GMT

←Older revision		Revision as of 12:03, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3qyq' size='340' side='right'caption='[[3qyq]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3qyq' size='340' side='right'caption='[[3qyq]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3qyq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Toxgm Toxgm~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QYQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3qyq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Toxoplasma_gondii_ME49 Toxoplasma gondii ME49]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QYQ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GLC:ALPHA~~-~~D-GLUCOSE'>GLC</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~TGME49_118750 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=508771 TOXGM])</td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900003:sucrose'>PRD_900003</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<~~tr id~~='~~activity~~'><~~td class="sblockLbl"~~><~~b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Deoxyribose-phosphate_aldolase Deoxyribose-phosphate aldolase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.4 4.1.2.4]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qyq OCA], [https://pdbe.org/3qyq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qyq RCSB], [https://www.ebi.ac.uk/pdbsum/3qyq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qyq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qyq OCA], [https://pdbe.org/3qyq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qyq RCSB], [https://www.ebi.ac.uk/pdbsum/3qyq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qyq ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/B9PVB4_TOXGV B9PVB4_TOXGV]
-	Parasites of the phylum Apicomplexa are highly successful pathogens of humans and animals world-wide. As obligate intracellular parasites, they have significant energy requirements for invasion and gliding motility that are supplied by various metabolic pathways. Aldolases have emerged as key enzymes involved in these pathways, and all apicomplexans express one or both of fructose 1,6-bisphosphate (F16BP) aldolase and 2-deoxyribose 5-phosphate (dR5P) aldolase (DERA). Intriguingly, Toxoplasma gondii, a highly successful apicomplexan parasite, expresses F16BP aldolase (TgALD1), d5RP aldolase (TgDERA), and a divergent dR5P aldolase-like protein (TgDPA) exclusively in the latent bradyzoite stage. While the importance of TgALD1 in glycolysis is well established and TgDERA is also likely to be involved in parasite metabolism, the detailed function of TgDPA remains elusive. To gain mechanistic insight into the function of different T. gondii aldolases, we first determined the crystal structures of TgALD1 and TgDPA. Structural analysis revealed that both aldolases adopt a TIM barrel fold accessorized with divergent secondary structure elements. Structural comparison of TgALD1 and TgDPA with members of their respective enzyme families revealed that while the active site residues are conserved in TgALD1, key catalytic residues are absent in TgDPA. Consistent with this observation, biochemical assays showed that while TgALD1 was active on F16BP, TgDPA was inactive on dR5P. Intriguingly, both aldolases are competent to bind polymerized actin in vitro. Altogether, structural and biochemical analyses of T. gondii aldolase and aldolase-like proteins reveal diverse functionalization of the classic TIM barrel aldolase fold.	+
-		+
-	~~Structural and functional divergence of the aldolase fold in Toxoplasma gondii.,Tonkin ML, Halavaty AS, Ramaswamy R, Ruan J, Igarashi M, Ngo HM, Boulanger MJ J Mol Biol. 2014 Oct 2. pii~~: ~~S0022-2836(14)00518-X. doi:, 10.1016~~/~~j.jmb.2014.09.019. PMID:25284756<ref>PMID:25284756<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3qyq" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Deoxyribose-phosphate aldolase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Toxgm~~]]	+	[[Category: Toxoplasma gondii ME49]]
-	[[Category: Anderson~~, W F]]~~	+	[[Category: Anderson WF]]
-	~~[[Category: Structural genomic~~]]	+	[[Category: Halavaty AS]]
-	[[Category: Halavaty~~, A S~~]]	+	[[Category: Igarashi M]]
-	[[Category: Igarashi, M]]	+	[[Category: Minasov G]]
-	[[Category: Minasov, G]]	+	[[Category: Ngo H]]
-	[[Category: Ngo, H]]	+	[[Category: Ruan J]]
-	[[Category: Ruan, J]]	+	[[Category: Shuvalova L]]
-	[[Category: Shuvalova, L]]	+	[[Category: Ueno A]]
-	[[Category: Ueno, A]]	+
-	~~[[Category: Bradyzoite]]~~	+
-	~~[[Category: Brain cyst]]~~	+
-	~~[[Category: Chemotherapy]]~~	+
-	~~[[Category: Csgid]]~~	+
-	~~[[Category: Lyase]]~~	+
-	~~[[Category: Tim-barrel~~]]	+

OCA at 05:41, 15 June 2022

OCA — Wed, 15 Jun 2022 05:41:49 GMT

←Older revision		Revision as of 05:41, 15 June 2022
Line 1:		Line 1:

	==1.8 Angstrom resolution crystal structure of a putative deoxyribose-phosphate aldolase from Toxoplasma gondii ME49==		==1.8 Angstrom resolution crystal structure of a putative deoxyribose-phosphate aldolase from Toxoplasma gondii ME49==
-	<StructureSection load='3qyq' size='340' side='right' caption='[[3qyq]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='3qyq' size='340' side='right'caption='[[3qyq]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3qyq]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Toxgm Toxgm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QYQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3QYQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3qyq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Toxgm Toxgm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QYQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=~~SUC~~:~~SUCROSE~~'>~~SUC~~</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TGME49_118750 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=508771 TOXGM])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TGME49_118750 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=508771 TOXGM])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Deoxyribose-phosphate_aldolase Deoxyribose-phosphate aldolase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.4 4.1.2.4] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Deoxyribose-phosphate_aldolase Deoxyribose-phosphate aldolase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.4 4.1.2.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3qyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qyq OCA], [~~http~~://pdbe.org/3qyq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3qyq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3qyq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3qyq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qyq OCA], [https://pdbe.org/3qyq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qyq RCSB], [https://www.ebi.ac.uk/pdbsum/3qyq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qyq ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Deoxyribose-phosphate aldolase]]		[[Category: Deoxyribose-phosphate aldolase]]
		+	[[Category: Large Structures]]
	[[Category: Toxgm]]		[[Category: Toxgm]]
	[[Category: Anderson, W F]]		[[Category: Anderson, W F]]

OCA at 09:20, 8 November 2017

OCA — Wed, 08 Nov 2017 09:20:21 GMT

←Older revision		Revision as of 09:20, 8 November 2017
Line 3:		Line 3:
	<StructureSection load='3qyq' size='340' side='right' caption='[[3qyq]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3qyq' size='340' side='right' caption='[[3qyq]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3qyq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Toxoplasma_gondii_me49 Toxoplasma gondii me49~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QYQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QYQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3qyq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Toxgm Toxgm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QYQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QYQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SUC:SUCROSE'>SUC</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SUC:SUCROSE'>SUC</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TGME49_118750 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=508771 ~~Toxoplasma gondii ME49~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TGME49_118750 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=508771 TOXGM])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxyribose-phosphate_aldolase Deoxyribose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.4 4.1.2.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxyribose-phosphate_aldolase Deoxyribose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.4 4.1.2.4] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qyq OCA], [http://pdbe.org/3qyq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3qyq RCSB], [http://www.ebi.ac.uk/pdbsum/3qyq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3qyq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qyq OCA], [http://pdbe.org/3qyq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3qyq RCSB], [http://www.ebi.ac.uk/pdbsum/3qyq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3qyq ProSAT]</span></td></tr>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Deoxyribose-phosphate aldolase]]		[[Category: Deoxyribose-phosphate aldolase]]
-	[[Category: ~~Toxoplasma gondii me49~~]]	+	[[Category: Toxgm]]
	[[Category: Anderson, W F]]		[[Category: Anderson, W F]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]

OCA at 17:24, 18 January 2017

OCA — Wed, 18 Jan 2017 17:24:59 GMT

←Older revision		Revision as of 17:24, 18 January 2017
Line 1:		Line 1:
		+
	==1.8 Angstrom resolution crystal structure of a putative deoxyribose-phosphate aldolase from Toxoplasma gondii ME49==		==1.8 Angstrom resolution crystal structure of a putative deoxyribose-phosphate aldolase from Toxoplasma gondii ME49==
	<StructureSection load='3qyq' size='340' side='right' caption='[[3qyq]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3qyq' size='340' side='right' caption='[[3qyq]], [[Resolution\|resolution]] 1.80Å' scene=''>
Line 6:		Line 7:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TGME49_118750 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=508771 Toxoplasma gondii ME49])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TGME49_118750 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=508771 Toxoplasma gondii ME49])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxyribose-phosphate_aldolase Deoxyribose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.4 4.1.2.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxyribose-phosphate_aldolase Deoxyribose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.4 4.1.2.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qyq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qyq RCSB], [http://www.ebi.ac.uk/pdbsum/3qyq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qyq OCA], [http://pdbe.org/3qyq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3qyq RCSB], [http://www.ebi.ac.uk/pdbsum/3qyq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3qyq ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3qyq" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:38, 25 January 2015

OCA — Sun, 25 Jan 2015 09:38:52 GMT

←Older revision		Revision as of 09:38, 25 January 2015
Line 2:		Line 2:
	<StructureSection load='3qyq' size='340' side='right' caption='[[3qyq]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3qyq' size='340' side='right' caption='[[3qyq]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3qyq]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QYQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QYQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3qyq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Toxoplasma_gondii_me49 Toxoplasma gondii me49]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QYQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QYQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SUC:SUCROSE'>SUC</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SUC:SUCROSE'>SUC</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TGME49_118750 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=508771 Toxoplasma gondii ME49])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxyribose-phosphate_aldolase Deoxyribose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.4 4.1.2.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxyribose-phosphate_aldolase Deoxyribose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.4 4.1.2.4] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qyq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qyq RCSB], [http://www.ebi.ac.uk/pdbsum/3qyq PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qyq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qyq RCSB], [http://www.ebi.ac.uk/pdbsum/3qyq PDBsum]</span></td></tr>
Line 20:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Deoxyribose-phosphate aldolase]]		[[Category: Deoxyribose-phosphate aldolase]]
		+	[[Category: Toxoplasma gondii me49]]
	[[Category: Anderson, W F]]		[[Category: Anderson, W F]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]

OCA at 07:29, 12 November 2014

OCA — Wed, 12 Nov 2014 07:29:04 GMT

←Older revision		Revision as of 07:29, 12 November 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3qyq\| PDB=3qyq \| SCENE= }}~~	+	==1.8 Angstrom resolution crystal structure of a putative deoxyribose-phosphate aldolase from Toxoplasma gondii ME49==
-	===1.8 Angstrom resolution crystal structure of a putative deoxyribose-phosphate aldolase from Toxoplasma gondii ME49===	+	<StructureSection load='3qyq' size='340' side='right' caption='[[3qyq]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3qyq]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QYQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QYQ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SUC:SUCROSE'>SUC</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Deoxyribose-phosphate_aldolase Deoxyribose-phosphate aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.4 4.1.2.4] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qyq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qyq RCSB], [http://www.ebi.ac.uk/pdbsum/3qyq PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Parasites of the phylum Apicomplexa are highly successful pathogens of humans and animals world-wide. As obligate intracellular parasites, they have significant energy requirements for invasion and gliding motility that are supplied by various metabolic pathways. Aldolases have emerged as key enzymes involved in these pathways, and all apicomplexans express one or both of fructose 1,6-bisphosphate (F16BP) aldolase and 2-deoxyribose 5-phosphate (dR5P) aldolase (DERA). Intriguingly, Toxoplasma gondii, a highly successful apicomplexan parasite, expresses F16BP aldolase (TgALD1), d5RP aldolase (TgDERA), and a divergent dR5P aldolase-like protein (TgDPA) exclusively in the latent bradyzoite stage. While the importance of TgALD1 in glycolysis is well established and TgDERA is also likely to be involved in parasite metabolism, the detailed function of TgDPA remains elusive. To gain mechanistic insight into the function of different T. gondii aldolases, we first determined the crystal structures of TgALD1 and TgDPA. Structural analysis revealed that both aldolases adopt a TIM barrel fold accessorized with divergent secondary structure elements. Structural comparison of TgALD1 and TgDPA with members of their respective enzyme families revealed that while the active site residues are conserved in TgALD1, key catalytic residues are absent in TgDPA. Consistent with this observation, biochemical assays showed that while TgALD1 was active on F16BP, TgDPA was inactive on dR5P. Intriguingly, both aldolases are competent to bind polymerized actin in vitro. Altogether, structural and biochemical analyses of T. gondii aldolase and aldolase-like proteins reveal diverse functionalization of the classic TIM barrel aldolase fold.

-	~~==About this Structure==~~	+	Structural and functional divergence of the aldolase fold in Toxoplasma gondii.,Tonkin ML, Halavaty AS, Ramaswamy R, Ruan J, Igarashi M, Ngo HM, Boulanger MJ J Mol Biol. 2014 Oct 2. pii: S0022-2836(14)00518-X. doi:, 10.1016/j.jmb.2014.09.019. PMID:25284756<ref>PMID:25284756</ref>
-	~~[[3qyq]] is a 4 chain structure with sequence from [http~~:~~//en~~.~~wikipedia~~.~~org~~/~~wiki/Toxoplasma_gondii_me49 Toxoplasma gondii me49]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id~~=~~3QYQ OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Deoxyribose-phosphate aldolase]]		[[Category: Deoxyribose-phosphate aldolase]]
-	~~[[Category: Toxoplasma gondii me49]]~~	+	[[Category: Anderson, W F]]
-	[[Category: Anderson, W F.]]	+	[[Category: Structural genomic]]
-	[[Category: ~~CSGID, Center for~~ Structural ~~Genomics of Infectious Diseases.~~]]	+	[[Category: Halavaty, A S]]
-	[[Category: Halavaty, A S.]]	+	[[Category: Igarashi, M]]
-	[[Category: Igarashi, M.]]	+	[[Category: Minasov, G]]
-	[[Category: Minasov, G.]]	+	[[Category: Ngo, H]]
-	[[Category: Ngo, H.]]	+	[[Category: Ruan, J]]
-	[[Category: Ruan, J.]]	+	[[Category: Shuvalova, L]]
-	[[Category: Shuvalova, L.]]	+	[[Category: Ueno, A]]
-	[[Category: Ueno, A.]]	+
	[[Category: Bradyzoite]]		[[Category: Bradyzoite]]
	[[Category: Brain cyst]]		[[Category: Brain cyst]]
-	[[Category: Center for structural genomics of infectious disease]]
	[[Category: Chemotherapy]]		[[Category: Chemotherapy]]
	[[Category: Csgid]]		[[Category: Csgid]]
	[[Category: Lyase]]		[[Category: Lyase]]
-	[[Category: Structural genomic]]
	[[Category: Tim-barrel]]		[[Category: Tim-barrel]]

OCA at 06:57, 15 January 2014

OCA — Wed, 15 Jan 2014 06:57:59 GMT

←Older revision		Revision as of 06:57, 15 January 2014
Line 3:		Line 3:

	==About this Structure==		==About this Structure==
-	[[3qyq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Toxoplasma_gondii~~ Toxoplasma gondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QYQ OCA].	+	[[3qyq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Toxoplasma_gondii_me49 Toxoplasma gondii me49]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QYQ OCA].
	[[Category: Deoxyribose-phosphate aldolase]]		[[Category: Deoxyribose-phosphate aldolase]]
-	[[Category: Toxoplasma gondii]]	+	[[Category: Toxoplasma gondii me49]]
	[[Category: Anderson, W F.]]		[[Category: Anderson, W F.]]
	[[Category: CSGID, Center for Structural Genomics of Infectious Diseases.]]		[[Category: CSGID, Center for Structural Genomics of Infectious Diseases.]]

OCA at 07:56, 16 May 2013

OCA — Thu, 16 May 2013 07:56:06 GMT

←Older revision		Revision as of 07:56, 16 May 2013
Line 1:		Line 1:
-	[[Image:3qyq.jpg\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3qyq", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3qyq\| PDB=3qyq \| SCENE= }}		{{STRUCTURE_3qyq\| PDB=3qyq \| SCENE= }}
-
	===1.8 Angstrom resolution crystal structure of a putative deoxyribose-phosphate aldolase from Toxoplasma gondii ME49===		===1.8 Angstrom resolution crystal structure of a putative deoxyribose-phosphate aldolase from Toxoplasma gondii ME49===
-

	==About this Structure==		==About this Structure==
Line 25:		Line 15:
	[[Category: Shuvalova, L.]]		[[Category: Shuvalova, L.]]
	[[Category: Ueno, A.]]		[[Category: Ueno, A.]]
		+	[[Category: Bradyzoite]]
		+	[[Category: Brain cyst]]
		+	[[Category: Center for structural genomics of infectious disease]]
		+	[[Category: Chemotherapy]]
		+	[[Category: Csgid]]
		+	[[Category: Lyase]]
		+	[[Category: Structural genomic]]
		+	[[Category: Tim-barrel]]

OCA at 08:19, 30 March 2011

OCA — Wed, 30 Mar 2011 08:19:04 GMT

←Older revision		Revision as of 08:19, 30 March 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3qyq.jpg\|left\|200px]]

-	The ~~entry 3qyq~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3qyq", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3qyq\| PDB=3qyq \| SCENE= }}

-	~~Authors: Halavaty, A~~.~~S., Ruan, J., Minasov, G., Shuvalova, L., Ueno, A., Igarashi, M., Anderson, W.F., Center for Structural Genomics~~ of ~~Infectious Diseases (CSGID)~~	+	===1.8 Angstrom resolution crystal structure of a putative deoxyribose-phosphate aldolase from Toxoplasma gondii ME49===

-	~~Description~~: 1.~~8 Angstrom resolution crystal structure of a putative deoxyribose~~-phosphate aldolase ~~from~~ Toxoplasma gondii ~~ME49~~	+
		+	==About this Structure==
		+	[[3qyq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QYQ OCA].
		+	[[Category: Deoxyribose-phosphate aldolase]]
		+	[[Category: Toxoplasma gondii]]
		+	[[Category: Anderson, W F.]]
		+	[[Category: CSGID, Center for Structural Genomics of Infectious Diseases.]]
		+	[[Category: Halavaty, A S.]]
		+	[[Category: Igarashi, M.]]
		+	[[Category: Minasov, G.]]
		+	[[Category: Ngo, H.]]
		+	[[Category: Ruan, J.]]
		+	[[Category: Shuvalova, L.]]
		+	[[Category: Ueno, A.]]

OCA: Protected "3qyq" [edit=sysop:move=sysop]

OCA — Wed, 09 Mar 2011 06:11:29 GMT

Protected "3qyq" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:11, 9 March 2011