3r2l - Revision history

OCA at 12:08, 14 March 2024

OCA — Thu, 14 Mar 2024 12:08:14 GMT

←Older revision		Revision as of 12:08, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3r2l' size='340' side='right'caption='[[3r2l]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='3r2l' size='340' side='right'caption='[[3r2l]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3r2l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R2L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3R2L FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3r2l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R2L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3R2L FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>~~, ~~<scene name='pdbligand=NA:SODIUM+ION'>NA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.85Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[3r2h\|3r2h]], [[3r2k\|3r2k]], [[3r2m\|3r2m]], [[3r2o\|3r2o]], [[3r2r\|3r2r]], [[3r2s\|3r2s]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">bfr, bfrA, PA4235 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3r2l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r2l OCA], [https://pdbe.org/3r2l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3r2l RCSB], [https://www.ebi.ac.uk/pdbsum/3r2l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3r2l ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3r2l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r2l OCA], [https://pdbe.org/3r2l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3r2l RCSB], [https://www.ebi.ac.uk/pdbsum/3r2l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3r2l ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/BFR_PSEAE BFR_PSEAE]] Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex.	+	[https://www.uniprot.org/uniprot/BFR_PSEAE BFR_PSEAE] Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Two distinct types of ferritin-like molecules often coexist in bacteria, the heme binding bacterioferritins (Bfr) and the non-heme binding bacterial ferritins (Ftn). The early isolation of a ferritin-like molecule from Pseudomonas aeruginosa suggested the possibility of a bacterioferritin assembled from two different subunits [Moore, G. R., et al. (1994) Biochem. J. 304, 493-497]. Subsequent studies demonstrated the presence of two genes encoding ferritin-like molecules in P. aeruginosa, designated bfrA and bfrB, and suggested that two distinct bacterioferritins may coexist [Ma, J.-F., et al. (1999) J. Bacteriol. 181, 3730-3742]. In this report, we present structural evidence demonstrating that the product of the bfrA gene is a ferritin-like molecule not capable of binding heme that harbors a catalytically active ferroxidase center with structural properties similar to those characteristic of bacterial and archaeal Ftns and clearly distinct from those of the ferroxidase center typical of Bfrs. Consequently, the product of the bfrA gene in P. aeruginosa is a bacterial ferritin, which we propose should be termed Pa FtnA. These results, together with the previous characterization of the product of the bfrB gene as a genuine bacterioferritin (Pa BfrB) [Weeratunga, S. J., et al. (2010) Biochemistry 49, 1160-1175], indicate the coexistence of a bacterial ferritin (Pa FtnA) and a bacterioferritin (Pa BfrB) in P. aeruginosa. In agreement with this idea, we also obtained evidence demonstrating that release of iron from Pa BfrB and Pa FtnA is likely subject to different regulation in P. aerugionsa. Whereas the efficient release of iron stored in Pa FtnA requires only the input of electrons from a ferredoxin NADP reductase (Pa Fpr), the release of iron stored in Pa BfrB requires not only electron delivery by Pa Fpr but also the presence of a "regulator", the apo form of a bacterioferritin-associated ferredoxin (apo Pa Bfd). Finally, structural analysis of iron uptake in crystallo suggests a possible pathway for the internalization of ferroxidase iron into the interior cavity of Pa FtnA.	+
-		+
-	Two Distinct Ferritin-like Molecules in Pseudomonas aeruginosa: The Product of the bfrA Gene Is a Bacterial Ferritin (FtnA) and Not a Bacterioferritin (Bfr).,Yao H, Jepkorir G, Lovell S, Nama PV, Weeratunga S, Battaile KP, Rivera M Biochemistry. 2011 May 20. PMID:21574546<ref>PMID:21574546</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3r2l" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Ferritin 3D structures\|Ferritin 3D structures]]		*[[Ferritin 3D structures\|Ferritin 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Battaile~~, K P~~]]	+	[[Category: Pseudomonas aeruginosa]]
-	[[Category: Jepkorir, G]]	+	[[Category: Battaile KP]]
-	[[Category: Lovell~~, S W~~]]	+	[[Category: Jepkorir G]]
-	[[Category: Nama~~, P V~~]]	+	[[Category: Lovell SW]]
-	[[Category: Rivera, M]]	+	[[Category: Nama PV]]
-	[[Category: Weeratunga, S]]	+	[[Category: Rivera M]]
-	[[Category: Yao, H]]	+	[[Category: Weeratunga S]]
-	~~[[Category: Bacterial ferritin]]~~	+	[[Category: Yao H]]
-	~~[[Category: Iron binding]]~~	+
-	~~[[Category: Iron homeostasis]]~~	+
-	~~[[Category: Iron mobilization]]~~	+
-	~~[[Category: Iron release]]~~	+
-	~~[[Category: Iron storage]]~~	+
-	~~[[Category: Metal binding protein~~]]	+

OCA at 05:46, 15 June 2022

OCA — Wed, 15 Jun 2022 05:46:23 GMT

←Older revision		Revision as of 05:46, 15 June 2022
Line 3:		Line 3:
	<StructureSection load='3r2l' size='340' side='right'caption='[[3r2l]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='3r2l' size='340' side='right'caption='[[3r2l]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3r2l]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R2L OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3R2L FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3r2l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R2L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3R2L FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3r2h\|3r2h]], [[3r2k\|3r2k]], [[3r2m\|3r2m]], [[3r2o\|3r2o]], [[3r2r\|3r2r]], [[3r2s\|3r2s]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3r2h\|3r2h]], [[3r2k\|3r2k]], [[3r2m\|3r2m]], [[3r2o\|3r2o]], [[3r2r\|3r2r]], [[3r2s\|3r2s]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bfr, bfrA, PA4235 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bfr, bfrA, PA4235 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3r2l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r2l OCA], [~~http~~://pdbe.org/3r2l PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3r2l RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3r2l PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3r2l ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3r2l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r2l OCA], [https://pdbe.org/3r2l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3r2l RCSB], [https://www.ebi.ac.uk/pdbsum/3r2l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3r2l ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/BFR_PSEAE BFR_PSEAE]] Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex.	+	[[https://www.uniprot.org/uniprot/BFR_PSEAE BFR_PSEAE]] Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 11:35, 17 July 2019

OCA — Wed, 17 Jul 2019 11:35:45 GMT

←Older revision		Revision as of 11:35, 17 July 2019
Line 1:		Line 1:

	==1.85A resolution structure of Iron Soaked FtnA from Pseudomonas aeruginosa (pH 7.5)==		==1.85A resolution structure of Iron Soaked FtnA from Pseudomonas aeruginosa (pH 7.5)==
-	<StructureSection load='3r2l' size='340' side='right' caption='[[3r2l]], [[Resolution\|resolution]] 1.85Å' scene=''>	+	<StructureSection load='3r2l' size='340' side='right'caption='[[3r2l]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3r2l]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R2L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3R2L FirstGlance]. <br>		<table><tr><td colspan='2'>[[3r2l]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R2L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3R2L FirstGlance]. <br>
Line 22:		Line 22:

	==See Also==		==See Also==
-	*[[Ferritin\|Ferritin]]	+	*[[Ferritin 3D structures\|Ferritin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Battaile, K P]]		[[Category: Battaile, K P]]
	[[Category: Jepkorir, G]]		[[Category: Jepkorir, G]]

OCA at 18:01, 4 August 2016

OCA — Thu, 04 Aug 2016 18:01:21 GMT

←Older revision		Revision as of 18:01, 4 August 2016
Line 1:		Line 1:
		+
	==1.85A resolution structure of Iron Soaked FtnA from Pseudomonas aeruginosa (pH 7.5)==		==1.85A resolution structure of Iron Soaked FtnA from Pseudomonas aeruginosa (pH 7.5)==
	<StructureSection load='3r2l' size='340' side='right' caption='[[3r2l]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='3r2l' size='340' side='right' caption='[[3r2l]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3r2l]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_aeruginosa Pseudomonas aeruginosa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R2L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3R2L FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3r2l]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R2L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3R2L FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3r2h\|3r2h]], [[3r2k\|3r2k]], [[3r2m\|3r2m]], [[3r2o\|3r2o]], [[3r2r\|3r2r]], [[3r2s\|3r2s]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3r2h\|3r2h]], [[3r2k\|3r2k]], [[3r2m\|3r2m]], [[3r2o\|3r2o]], [[3r2r\|3r2r]], [[3r2s\|3r2s]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bfr, bfrA, PA4235 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 ~~Pseudomonas aeruginosa~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bfr, bfrA, PA4235 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3r2l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r2l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3r2l RCSB], [http://www.ebi.ac.uk/pdbsum/3r2l PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3r2l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r2l OCA], [http://pdbe.org/3r2l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3r2l RCSB], [http://www.ebi.ac.uk/pdbsum/3r2l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3r2l ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BFR_PSEAE BFR_PSEAE]] Iron-storage protein, whose ferroxidase center binds Fe(2+) ions, oxidizes them by dioxygen to Fe(3+), and participates in the subsequent Fe(3+) oxide mineral core formation within the central cavity of the protein complex.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 16:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3r2l" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 23:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Pseudomonas aeruginosa]]
	[[Category: Battaile, K P]]		[[Category: Battaile, K P]]
	[[Category: Jepkorir, G]]		[[Category: Jepkorir, G]]

OCA at 22:35, 3 January 2015

OCA — Sat, 03 Jan 2015 22:35:04 GMT

←Older revision		Revision as of 22:35, 3 January 2015
Line 1:		Line 1:
-	[[~~Image:~~3r2l.~~png~~\|~~left~~\|~~200px~~]]	+	==1.85A resolution structure of Iron Soaked FtnA from Pseudomonas aeruginosa (pH 7.5)==
		+	<StructureSection load='3r2l' size='340' side='right' caption='[[3r2l]], [[Resolution\|resolution]] 1.85Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3r2l]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R2L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3R2L FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3r2h\|3r2h]], [[3r2k\|3r2k]], [[3r2m\|3r2m]], [[3r2o\|3r2o]], [[3r2r\|3r2r]], [[3r2s\|3r2s]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bfr, bfrA, PA4235 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3r2l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r2l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3r2l RCSB], [http://www.ebi.ac.uk/pdbsum/3r2l PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Two distinct types of ferritin-like molecules often coexist in bacteria, the heme binding bacterioferritins (Bfr) and the non-heme binding bacterial ferritins (Ftn). The early isolation of a ferritin-like molecule from Pseudomonas aeruginosa suggested the possibility of a bacterioferritin assembled from two different subunits [Moore, G. R., et al. (1994) Biochem. J. 304, 493-497]. Subsequent studies demonstrated the presence of two genes encoding ferritin-like molecules in P. aeruginosa, designated bfrA and bfrB, and suggested that two distinct bacterioferritins may coexist [Ma, J.-F., et al. (1999) J. Bacteriol. 181, 3730-3742]. In this report, we present structural evidence demonstrating that the product of the bfrA gene is a ferritin-like molecule not capable of binding heme that harbors a catalytically active ferroxidase center with structural properties similar to those characteristic of bacterial and archaeal Ftns and clearly distinct from those of the ferroxidase center typical of Bfrs. Consequently, the product of the bfrA gene in P. aeruginosa is a bacterial ferritin, which we propose should be termed Pa FtnA. These results, together with the previous characterization of the product of the bfrB gene as a genuine bacterioferritin (Pa BfrB) [Weeratunga, S. J., et al. (2010) Biochemistry 49, 1160-1175], indicate the coexistence of a bacterial ferritin (Pa FtnA) and a bacterioferritin (Pa BfrB) in P. aeruginosa. In agreement with this idea, we also obtained evidence demonstrating that release of iron from Pa BfrB and Pa FtnA is likely subject to different regulation in P. aerugionsa. Whereas the efficient release of iron stored in Pa FtnA requires only the input of electrons from a ferredoxin NADP reductase (Pa Fpr), the release of iron stored in Pa BfrB requires not only electron delivery by Pa Fpr but also the presence of a "regulator", the apo form of a bacterioferritin-associated ferredoxin (apo Pa Bfd). Finally, structural analysis of iron uptake in crystallo suggests a possible pathway for the internalization of ferroxidase iron into the interior cavity of Pa FtnA.

-	~~{{STRUCTURE_3r2l\| PDB=3r2l \| SCENE= }}~~	+	Two Distinct Ferritin-like Molecules in Pseudomonas aeruginosa: The Product of the bfrA Gene Is a Bacterial Ferritin (FtnA) and Not a Bacterioferritin (Bfr).,Yao H, Jepkorir G, Lovell S, Nama PV, Weeratunga S, Battaile KP, Rivera M Biochemistry. 2011 May 20. PMID:21574546<ref>PMID:21574546</ref>

-	~~===1~~.~~85A resolution structure~~ of ~~Iron Soaked FtnA from Pseudomonas aeruginosa (pH 7~~.~~5)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_21574546}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3r2l]] is a 1 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R2L OCA].~~	+

	==See Also==		==See Also==
	*[[Ferritin\|Ferritin]]		*[[Ferritin\|Ferritin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:021574546<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
-	[[Category: Battaile, K P.]]	+	[[Category: Battaile, K P]]
-	[[Category: Jepkorir, G.]]	+	[[Category: Jepkorir, G]]
-	[[Category: Lovell, S W.]]	+	[[Category: Lovell, S W]]
-	[[Category: Nama, P V.]]	+	[[Category: Nama, P V]]
-	[[Category: Rivera, M.]]	+	[[Category: Rivera, M]]
-	[[Category: Weeratunga, S.]]	+	[[Category: Weeratunga, S]]
-	[[Category: Yao, H.]]	+	[[Category: Yao, H]]
	[[Category: Bacterial ferritin]]		[[Category: Bacterial ferritin]]
	[[Category: Iron binding]]		[[Category: Iron binding]]

OCA at 16:16, 20 October 2012

OCA — Sat, 20 Oct 2012 16:16:55 GMT

←Older revision		Revision as of 16:16, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3r2l]] is a 1 chain structure ~~of [[Ferritin]]~~ with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R2L OCA].	+	[[3r2l]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R2L OCA].

	==See Also==		==See Also==

OCA at 21:09, 26 July 2012

OCA — Thu, 26 Jul 2012 21:09:50 GMT

←Older revision		Revision as of 21:09, 26 July 2012
Line 1:		Line 1:
	[[Image:3r2l.png\|left\|200px]]		[[Image:3r2l.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3r2l", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3r2l\| PDB=3r2l \| SCENE= }}		{{STRUCTURE_3r2l\| PDB=3r2l \| SCENE= }}

	===1.85A resolution structure of Iron Soaked FtnA from Pseudomonas aeruginosa (pH 7.5)===		===1.85A resolution structure of Iron Soaked FtnA from Pseudomonas aeruginosa (pH 7.5)===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21574546}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 21574546 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_21574546}}		{{ABSTRACT_PUBMED_21574546}}

Line 22:		Line 11:

	==See Also==		==See Also==
-	*[[Ferritin]]	+	*[[Ferritin\|Ferritin]]

	==Reference==		==Reference==

OCA at 07:29, 29 February 2012

OCA — Wed, 29 Feb 2012 07:29:26 GMT

←Older revision		Revision as of 07:29, 29 February 2012
Line 1:		Line 1:
-	[[Image:3r2l.~~jpg~~\|left\|200px]]	+	[[Image:3r2l.png\|left\|200px]]

	<!--		<!--
Line 19:		Line 19:

	==About this Structure==		==About this Structure==
-	[[3r2l]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R2L OCA].	+	[[3r2l]] is a 1 chain structure of [[Ferritin]] with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R2L OCA].
		+
		+	==See Also==
		+	*[[Ferritin]]

	==Reference==		==Reference==
Line 31:		Line 34:
	[[Category: Weeratunga, S.]]		[[Category: Weeratunga, S.]]
	[[Category: Yao, H.]]		[[Category: Yao, H.]]
		+	[[Category: Bacterial ferritin]]
		+	[[Category: Iron binding]]
		+	[[Category: Iron homeostasis]]
		+	[[Category: Iron mobilization]]
		+	[[Category: Iron release]]
		+	[[Category: Iron storage]]
		+	[[Category: Metal binding protein]]

OCA at 05:32, 25 May 2011

OCA — Wed, 25 May 2011 05:32:22 GMT

←Older revision		Revision as of 05:32, 25 May 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3r2l.jpg\|left\|200px]]

-	The ~~entry 3r2l~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3r2l", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3r2l\| PDB=3r2l \| SCENE= }}

-	~~Authors: Lovell, S~~.~~, Battaile, K.P., Yao, H., Jepkorir, G., Nama, P.V., Weeratunga, S., Rivera, M~~.	+	===1.85A resolution structure of Iron Soaked FtnA from Pseudomonas aeruginosa (pH 7.5)===

-	~~Description~~: 1.~~85A resolution~~ structure ~~of Iron Soaked FtnA~~ from Pseudomonas aeruginosa ~~(pH 7~~.5)	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21574546}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21574546 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21574546}}
		+
		+	==About this Structure==
		+	[[3r2l]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R2L OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021574546</ref><references group="xtra"/>
		+	[[Category: Pseudomonas aeruginosa]]
		+	[[Category: Battaile, K P.]]
		+	[[Category: Jepkorir, G.]]
		+	[[Category: Lovell, S W.]]
		+	[[Category: Nama, P V.]]
		+	[[Category: Rivera, M.]]
		+	[[Category: Weeratunga, S.]]
		+	[[Category: Yao, H.]]

OCA at 07:47, 30 March 2011

OCA — Wed, 30 Mar 2011 07:47:13 GMT

←Older revision		Revision as of 07:47, 30 March 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3r2l is ON HOLD	+	The entry 3r2l is ON HOLD until Paper Publication

	Authors: Lovell, S., Battaile, K.P., Yao, H., Jepkorir, G., Nama, P.V., Weeratunga, S., Rivera, M.		Authors: Lovell, S., Battaile, K.P., Yao, H., Jepkorir, G., Nama, P.V., Weeratunga, S., Rivera, M.

	Description: 1.85A resolution structure of Iron Soaked FtnA from Pseudomonas aeruginosa (pH 7.5)		Description: 1.85A resolution structure of Iron Soaked FtnA from Pseudomonas aeruginosa (pH 7.5)