3r4c - Revision history

OCA at 12:10, 14 March 2024

OCA — Thu, 14 Mar 2024 12:10:16 GMT

←Older revision		Revision as of 12:10, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3r4c' size='340' side='right'caption='[[3r4c]], [[Resolution\|resolution]] 1.82Å' scene=''>		<StructureSection load='3r4c' size='340' side='right'caption='[[3r4c]], [[Resolution\|resolution]] 1.82Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3r4c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_thetaiotaomicron"_distaso_1912 "bacillus~~ thetaiotaomicron~~" distaso 1912~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R4C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3R4C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3r4c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteroides_thetaiotaomicron Bacteroides thetaiotaomicron]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R4C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3R4C FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.82Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~BT1666, BT_1666 ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=818 "Bacillus thetaiotaomicron" Distaso 1912])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3r4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r4c OCA], [https://pdbe.org/3r4c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3r4c RCSB], [https://www.ebi.ac.uk/pdbsum/3r4c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3r4c ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3r4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r4c OCA], [https://pdbe.org/3r4c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3r4c RCSB], [https://www.ebi.ac.uk/pdbsum/3r4c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3r4c ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q8A759_BACTN Q8A759_BACTN]
-	Analysis of the haloalkanoate dehalogenase superfamily (HADSF) has uncovered homologues occurring within the same organism that are found to possess broad, overlapping substrate specificities, and low catalytic efficiencies. Here we compare the HADSF phosphatase BT1666 from Bacteroides thetaiotaomicron VPI-5482 to a homologue with high sequence identity (40%) from the same organism BT4131, a known hexose-phosphate phosphatase. The goal is to find whether these enzymes represent duplicated versus paralogous activities. The X-ray crystal structure of BT1666 was determined to 1.82 A resolution. Superposition of the BT1666 and BT4131 structures revealed a conserved fold and identical active sites suggestive of a common physiological substrate. The steady-state kinetic constants for BT1666 were determined for a diverse panel of phosphorylated metabolites to define its substrate specificity profile and overall level of catalytic efficiency. Whereas BT1666 and BT4131 are both promiscuous, their substrate specificity profiles are distinct. The catalytic efficiency of BT1666 (k(cat) /K(m) = 4.4 x 10(2) M(-1) s(-1) for the best substrate fructose 1,6-(bis)phosphate) is an order of magnitude less than that of BT4131 (k(cat) /K(m) = 6.7 x 10(3) M(-1) s(-1) for 2-deoxyglucose 6-phosphate). The seemingly identical active-site structures point to sequence variation outside the active site causing differences in conformational dynamics or subtle catalytic positioning effects that drive the divergence in catalytic efficiency and selectivity. The overlapping substrate profiles may be understood in terms of differential regulation of expression of the two enzymes or a conferred advantage in metabolic housekeeping functions by having a larger range of possible metabolites as substrates. Proteins 2011;. (c) 2011 Wiley-Liss, Inc.	+
-		+
-	~~The X-ray crystallographic structure and specificity profile of HAD superfamily phosphohydrolase BT1666~~: ~~Comparison of paralogous functions in B. thetaiotaomicron.,Lu Z, Dunaway-Mariano D, Allen KN Proteins. 2011 Nov;79(11):3099-107. doi: 10.1002~~/~~prot.23137. Epub 2011 Aug, 30. PMID:21989931<ref>PMID:21989931<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3r4c" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ thetaiotaomicron ~~distaso 1912~~]]	+	[[Category: Bacteroides thetaiotaomicron]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Allen~~, K N~~]]	+	[[Category: Allen KN]]
-	[[Category: Dunaway-Mariano, D]]	+	[[Category: Dunaway-Mariano D]]
-	[[Category: Lu, Z]]	+	[[Category: Lu Z]]
-	~~[[Category: Haloalkanoate dehalogenase enzyme superfamily]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Phosphohydrolase~~]]	+

OCA at 05:48, 15 June 2022

OCA — Wed, 15 Jun 2022 05:48:29 GMT

←Older revision		Revision as of 05:48, 15 June 2022
Line 1:		Line 1:

	==Divergence of Structure and Function Among Phosphatases of the Haloalkanoate (HAD) Enzyme Superfamily: Analysis of BT1666 from Bacteroides thetaiotaomicron==		==Divergence of Structure and Function Among Phosphatases of the Haloalkanoate (HAD) Enzyme Superfamily: Analysis of BT1666 from Bacteroides thetaiotaomicron==
-	<StructureSection load='3r4c' size='340' side='right' caption='[[3r4c]], [[Resolution\|resolution]] 1.82Å' scene=''>	+	<StructureSection load='3r4c' size='340' side='right'caption='[[3r4c]], [[Resolution\|resolution]] 1.82Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3r4c]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_thetaiotaomicron"_distaso_1912 "bacillus thetaiotaomicron" distaso 1912]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R4C OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3R4C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3r4c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_thetaiotaomicron"_distaso_1912 "bacillus thetaiotaomicron" distaso 1912]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R4C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3R4C FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BT1666, BT_1666 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=818 "Bacillus thetaiotaomicron" Distaso 1912])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BT1666, BT_1666 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=818 "Bacillus thetaiotaomicron" Distaso 1912])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3r4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r4c OCA], [~~http~~://pdbe.org/3r4c PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3r4c RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3r4c PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3r4c ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3r4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r4c OCA], [https://pdbe.org/3r4c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3r4c RCSB], [https://www.ebi.ac.uk/pdbsum/3r4c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3r4c ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus thetaiotaomicron distaso 1912]]		[[Category: Bacillus thetaiotaomicron distaso 1912]]
		+	[[Category: Large Structures]]
	[[Category: Allen, K N]]		[[Category: Allen, K N]]
	[[Category: Dunaway-Mariano, D]]		[[Category: Dunaway-Mariano, D]]

OCA at 19:16, 4 August 2016

OCA — Thu, 04 Aug 2016 19:16:56 GMT

←Older revision		Revision as of 19:16, 4 August 2016
Line 1:		Line 1:
		+
	==Divergence of Structure and Function Among Phosphatases of the Haloalkanoate (HAD) Enzyme Superfamily: Analysis of BT1666 from Bacteroides thetaiotaomicron==		==Divergence of Structure and Function Among Phosphatases of the Haloalkanoate (HAD) Enzyme Superfamily: Analysis of BT1666 from Bacteroides thetaiotaomicron==
	<StructureSection load='3r4c' size='340' side='right' caption='[[3r4c]], [[Resolution\|resolution]] 1.82Å' scene=''>		<StructureSection load='3r4c' size='340' side='right' caption='[[3r4c]], [[Resolution\|resolution]] 1.82Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3r4c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacteroides_thetaiotaomicron Bacteroides~~ thetaiotaomicron]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R4C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3R4C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3r4c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_thetaiotaomicron"_distaso_1912 "bacillus thetaiotaomicron" distaso 1912]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R4C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3R4C FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BT1666, BT_1666 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=818 ~~Bacteroides~~ thetaiotaomicron])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BT1666, BT_1666 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=818 "Bacillus thetaiotaomicron" Distaso 1912])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3r4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r4c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3r4c RCSB], [http://www.ebi.ac.uk/pdbsum/3r4c PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3r4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r4c OCA], [http://pdbe.org/3r4c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3r4c RCSB], [http://www.ebi.ac.uk/pdbsum/3r4c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3r4c ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3r4c" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacteroides~~ thetaiotaomicron]]	+	[[Category: Bacillus thetaiotaomicron distaso 1912]]
	[[Category: Allen, K N]]		[[Category: Allen, K N]]
	[[Category: Dunaway-Mariano, D]]		[[Category: Dunaway-Mariano, D]]

OCA at 21:26, 3 January 2015

OCA — Sat, 03 Jan 2015 21:26:35 GMT

←Older revision		Revision as of 21:26, 3 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3r4c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteroides_thetaiotaomicron Bacteroides thetaiotaomicron]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R4C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3R4C FirstGlance]. <br>		<table><tr><td colspan='2'>[[3r4c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteroides_thetaiotaomicron Bacteroides thetaiotaomicron]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R4C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3R4C FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BT1666, BT_1666 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=818 Bacteroides thetaiotaomicron])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BT1666, BT_1666 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=818 Bacteroides thetaiotaomicron])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3r4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r4c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3r4c RCSB], [http://www.ebi.ac.uk/pdbsum/3r4c PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3r4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r4c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3r4c RCSB], [http://www.ebi.ac.uk/pdbsum/3r4c PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 13:		Line 13:
	The X-ray crystallographic structure and specificity profile of HAD superfamily phosphohydrolase BT1666: Comparison of paralogous functions in B. thetaiotaomicron.,Lu Z, Dunaway-Mariano D, Allen KN Proteins. 2011 Nov;79(11):3099-107. doi: 10.1002/prot.23137. Epub 2011 Aug, 30. PMID:21989931<ref>PMID:21989931</ref>		The X-ray crystallographic structure and specificity profile of HAD superfamily phosphohydrolase BT1666: Comparison of paralogous functions in B. thetaiotaomicron.,Lu Z, Dunaway-Mariano D, Allen KN Proteins. 2011 Nov;79(11):3099-107. doi: 10.1002/prot.23137. Epub 2011 Aug, 30. PMID:21989931<ref>PMID:21989931</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 20:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Bacteroides thetaiotaomicron]]		[[Category: Bacteroides thetaiotaomicron]]
-	[[Category: Allen, K N.]]	+	[[Category: Allen, K N]]
-	[[Category: Dunaway-Mariano, D.]]	+	[[Category: Dunaway-Mariano, D]]
-	[[Category: Lu, Z.]]	+	[[Category: Lu, Z]]
	[[Category: Haloalkanoate dehalogenase enzyme superfamily]]		[[Category: Haloalkanoate dehalogenase enzyme superfamily]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Phosphohydrolase]]		[[Category: Phosphohydrolase]]

OCA at 04:48, 5 June 2014

OCA — Thu, 05 Jun 2014 04:48:42 GMT

←Older revision		Revision as of 04:48, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3r4c.~~png~~\|~~left~~\|~~200px~~]]	+	==Divergence of Structure and Function Among Phosphatases of the Haloalkanoate (HAD) Enzyme Superfamily: Analysis of BT1666 from Bacteroides thetaiotaomicron==
		+	<StructureSection load='3r4c' size='340' side='right' caption='[[3r4c]], [[Resolution\|resolution]] 1.82Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3r4c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteroides_thetaiotaomicron Bacteroides thetaiotaomicron]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R4C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3R4C FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BT1666, BT_1666 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=818 Bacteroides thetaiotaomicron])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3r4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r4c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3r4c RCSB], [http://www.ebi.ac.uk/pdbsum/3r4c PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Analysis of the haloalkanoate dehalogenase superfamily (HADSF) has uncovered homologues occurring within the same organism that are found to possess broad, overlapping substrate specificities, and low catalytic efficiencies. Here we compare the HADSF phosphatase BT1666 from Bacteroides thetaiotaomicron VPI-5482 to a homologue with high sequence identity (40%) from the same organism BT4131, a known hexose-phosphate phosphatase. The goal is to find whether these enzymes represent duplicated versus paralogous activities. The X-ray crystal structure of BT1666 was determined to 1.82 A resolution. Superposition of the BT1666 and BT4131 structures revealed a conserved fold and identical active sites suggestive of a common physiological substrate. The steady-state kinetic constants for BT1666 were determined for a diverse panel of phosphorylated metabolites to define its substrate specificity profile and overall level of catalytic efficiency. Whereas BT1666 and BT4131 are both promiscuous, their substrate specificity profiles are distinct. The catalytic efficiency of BT1666 (k(cat) /K(m) = 4.4 x 10(2) M(-1) s(-1) for the best substrate fructose 1,6-(bis)phosphate) is an order of magnitude less than that of BT4131 (k(cat) /K(m) = 6.7 x 10(3) M(-1) s(-1) for 2-deoxyglucose 6-phosphate). The seemingly identical active-site structures point to sequence variation outside the active site causing differences in conformational dynamics or subtle catalytic positioning effects that drive the divergence in catalytic efficiency and selectivity. The overlapping substrate profiles may be understood in terms of differential regulation of expression of the two enzymes or a conferred advantage in metabolic housekeeping functions by having a larger range of possible metabolites as substrates. Proteins 2011;. (c) 2011 Wiley-Liss, Inc.

-	~~<!--~~	+	The X-ray crystallographic structure and specificity profile of HAD superfamily phosphohydrolase BT1666: Comparison of paralogous functions in B. thetaiotaomicron.,Lu Z, Dunaway-Mariano D, Allen KN Proteins. 2011 Nov;79(11):3099-107. doi: 10.1002/prot.23137. Epub 2011 Aug, 30. PMID:21989931<ref>PMID:21989931</ref>
-	The ~~line below this paragraph~~, ~~containing "STRUCTURE_3r4c"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded~~),	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3r4c\| PDB=3r4c \| SCENE= }}~~	+

-	~~===Divergence of Structure and Function Among Phosphatases~~ of the ~~Haloalkanoate (HAD) Enzyme Superfamily: Analysis of BT1666 from Bacteroides thetaiotaomicron===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_21989931}}, adds the Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 21989931 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_21989931}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3r4c]] is a 1 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Bacteroides_thetaiotaomicron Bacteroides thetaiotaomicron]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R4C OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:021989931<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Bacteroides thetaiotaomicron]]		[[Category: Bacteroides thetaiotaomicron]]
	[[Category: Allen, K N.]]		[[Category: Allen, K N.]]

OCA at 09:16, 30 October 2011

OCA — Sun, 30 Oct 2011 09:16:57 GMT

←Older revision		Revision as of 09:16, 30 October 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3r4c.png\|left\|200px]]

-	The ~~entry 3r4c~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3r4c", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3r4c\| PDB=3r4c \| SCENE= }}

-	~~Authors~~: ~~Allen, K.N., Lu, Z., Dunaway-Mariano, D.~~	+	===Divergence of Structure and Function Among Phosphatases of the Haloalkanoate (HAD) Enzyme Superfamily: Analysis of BT1666 from Bacteroides thetaiotaomicron===

-	~~Description: Divergence of Structure and Function Among Phosphatases of~~ the ~~Haloalkanoate~~ (~~HAD~~) ~~Enzyme Superfamily~~: ~~Analysis of BT1666~~ from Bacteroides thetaiotaomicron	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21989931}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21989931 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21989931}}
		+
		+	==About this Structure==
		+	[[3r4c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteroides_thetaiotaomicron Bacteroides thetaiotaomicron]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R4C OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021989931</ref><references group="xtra"/>
		+	[[Category: Bacteroides thetaiotaomicron]]
		+	[[Category: Allen, K N.]]
		+	[[Category: Dunaway-Mariano, D.]]
		+	[[Category: Lu, Z.]]
		+	[[Category: Haloalkanoate dehalogenase enzyme superfamily]]
		+	[[Category: Hydrolase]]
		+	[[Category: Phosphohydrolase]]

OCA at 06:53, 27 April 2011

OCA — Wed, 27 Apr 2011 06:53:09 GMT

←Older revision		Revision as of 06:53, 27 April 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3r4c is ON HOLD	+	The entry 3r4c is ON HOLD until Paper Publication

	Authors: Allen, K.N., Lu, Z., Dunaway-Mariano, D.		Authors: Allen, K.N., Lu, Z., Dunaway-Mariano, D.

	Description: Divergence of Structure and Function Among Phosphatases of the Haloalkanoate (HAD) Enzyme Superfamily: Analysis of BT1666 from Bacteroides thetaiotaomicron		Description: Divergence of Structure and Function Among Phosphatases of the Haloalkanoate (HAD) Enzyme Superfamily: Analysis of BT1666 from Bacteroides thetaiotaomicron

OCA: Protected "3r4c" [edit=sysop:move=sysop]

OCA — Wed, 23 Mar 2011 06:51:49 GMT

Protected "3r4c" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:51, 23 March 2011

OCA: New page: '''Unreleased structure''' The entry 3r4c is ON HOLD Authors: Allen, K.N., Lu, Z., Dunaway-Mariano, D. Description: Divergence of Structure and Function Among Phosphatases of the Haloa...

OCA — Wed, 23 Mar 2011 06:51:48 GMT

New page: '''Unreleased structure''' The entry 3r4c is ON HOLD Authors: Allen, K.N., Lu, Z., Dunaway-Mariano, D. Description: Divergence of Structure and Function Among Phosphatases of the Haloa...

New page

'''Unreleased structure'''

The entry 3r4c is ON HOLD

Authors: Allen, K.N., Lu, Z., Dunaway-Mariano, D.

Description: Divergence of Structure and Function Among Phosphatases of the Haloalkanoate (HAD) Enzyme Superfamily: Analysis of BT1666 from Bacteroides thetaiotaomicron