3r60 - Revision history

OCA at 10:52, 21 February 2024

OCA — Wed, 21 Feb 2024 10:52:35 GMT

←Older revision		Revision as of 10:52, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='3r60' size='340' side='right'caption='[[3r60]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3r60' size='340' side='right'caption='[[3r60]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3r60]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"vibrio_subtilis"_ehrenberg_1835 "vibrio~~ subtilis~~" ehrenberg 1835~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R60 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3R60 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3r60]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R60 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3R60 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=PGO:S-1,2-PROPANEDIOL'>PGO</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3r61\|3r61]], [[2f5d\|2f5d]], [[2ev6\|2ev6]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=PGO:S-1,2-PROPANEDIOL'>PGO</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Bacillus subtilis, BSU24520, mntR, yqhN ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3r60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r60 OCA], [https://pdbe.org/3r60 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3r60 RCSB], [https://www.ebi.ac.uk/pdbsum/3r60 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3r60 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3r60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r60 OCA], [https://pdbe.org/3r60 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3r60 RCSB], [https://www.ebi.ac.uk/pdbsum/3r60 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3r60 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/MNTR_BACSU MNTR_BACSU]] Central regulator of manganese homeostasis. In the presence of manganese, it mediates repression of the manganese transporter MntH; under low manganese conditions, it activates the transcription of the mntABCD operon.	+	[https://www.uniprot.org/uniprot/MNTR_BACSU MNTR_BACSU] Central regulator of manganese homeostasis. In the presence of manganese, it mediates repression of the manganese transporter MntH; under low manganese conditions, it activates the transcription of the mntABCD operon.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The manganese transport regulator (MntR) represses the expression of genes involved in manganese uptake in Bacillus subtilis. It selectively responds to Mn(2+) and Cd(2+) over other divalent metal cations, including Fe(2+), Co(2+), and Zn(2+). Previous work has shown that MntR forms binuclear complexes with Mn(2+) or Cd(2+) at two binding sites, labeled A and C, that are separated by 4.4 A. Zinc activates MntR poorly and binds only to the A site, forming a mononuclear complex. The difference in metal binding stoichiometry suggested a mechanism for selectivity in MntR. Larger metal cations are strongly activating because they can form the binuclear complex, while smaller metal ions cannot bind with the geometry needed to fully occupy both metal binding sites. To investigate this hypothesis, structures of MntR in complex with two other noncognate metal ions, Fe(2+) and Co(2+), have been determined. Each metal forms a mononuclear complex with MntR with the metal ion bound in the A site, supporting the conclusions drawn from the Zn(2+) complex. Additionally, we investigated two site-specific mutants of MntR, E11K and H77A, that contain substitutions of metal binding residues in the A site. While metal binding in each mutant is significantly altered relative to that of wild-type MntR, both mutants retain activity and selectivity for Mn(2+) in vitro and in vivo. That observation, coupled with previous studies, suggests that the A and C sites both contribute to the selectivity of MntR.	+
-		+
-	Roles of the A and C Sites in the Manganese-Specific Activation of MntR.,McGuire AM, Cuthbert BJ, Ma Z, Grauer-Gray KD, Brunjes Brophy M, Spear KA, Soonsanga S, Kliegman JI, Griner SL, Helmann JD, Glasfeld A Biochemistry. 2013 Jan 29;52(4):701-13. doi: 10.1021/bi301550t. Epub 2013 Jan 17. PMID:23298157<ref>PMID:23298157</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3r60" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Vibrio~~ subtilis ~~ehrenberg 1835~~]]	+	[[Category: Bacillus subtilis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Brophy~~, M B~~]]	+	[[Category: Brophy MB]]
-	[[Category: Glasfeld, A]]	+	[[Category: Glasfeld A]]
-	[[Category: Griner~~, S L~~]]	+	[[Category: Griner SL]]
-	[[Category: Kliegman~~, J I~~]]	+	[[Category: Kliegman JI]]
-	[[Category: Nix~~, J C]]~~	+	[[Category: Nix JC]]
-	~~[[Category: Transcription regulator]]~~	+
-	~~[[Category: Winged helix-turn-helix~~]]	+

OCA at 05:50, 15 June 2022

OCA — Wed, 15 Jun 2022 05:50:25 GMT

←Older revision		Revision as of 05:50, 15 June 2022
Line 1:		Line 1:

	==Structure of the MntR Fe2+ Complex==		==Structure of the MntR Fe2+ Complex==
-	<StructureSection load='3r60' size='340' side='right' caption='[[3r60]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='3r60' size='340' side='right'caption='[[3r60]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3r60]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"~~bacillus_globigii~~"~~_migula_1900~~ "~~bacillus globigii~~" ~~migula 1900~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R60 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3R60 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3r60]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R60 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3R60 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=PGO:S-1,2-PROPANEDIOL'>PGO</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=PGO:S-1,2-PROPANEDIOL'>PGO</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3r61\|3r61]], [[2f5d\|2f5d]], [[2ev6\|2ev6]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3r61\|3r61]], [[2f5d\|2f5d]], [[2ev6\|2ev6]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Bacillus subtilis, BSU24520, mntR, yqhN ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "~~Bacillus globigii~~" ~~Migula 1900~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Bacillus subtilis, BSU24520, mntR, yqhN ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3r60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r60 OCA], [~~http~~://pdbe.org/3r60 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3r60 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3r60 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3r60 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3r60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r60 OCA], [https://pdbe.org/3r60 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3r60 RCSB], [https://www.ebi.ac.uk/pdbsum/3r60 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3r60 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/MNTR_BACSU MNTR_BACSU]] Central regulator of manganese homeostasis. In the presence of manganese, it mediates repression of the manganese transporter MntH; under low manganese conditions, it activates the transcription of the mntABCD operon.	+	[[https://www.uniprot.org/uniprot/MNTR_BACSU MNTR_BACSU]] Central regulator of manganese homeostasis. In the presence of manganese, it mediates repression of the manganese transporter MntH; under low manganese conditions, it activates the transcription of the mntABCD operon.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus globigii migula 1900~~]]	+	[[Category: Vibrio subtilis ehrenberg 1835]]
		+	[[Category: Large Structures]]
	[[Category: Brophy, M B]]		[[Category: Brophy, M B]]
	[[Category: Glasfeld, A]]		[[Category: Glasfeld, A]]

OCA at 20:05, 4 August 2016

OCA — Thu, 04 Aug 2016 20:05:26 GMT

←Older revision		Revision as of 20:05, 4 August 2016
Line 1:		Line 1:
		+
	==Structure of the MntR Fe2+ Complex==		==Structure of the MntR Fe2+ Complex==
	<StructureSection load='3r60' size='340' side='right' caption='[[3r60]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3r60' size='340' side='right' caption='[[3r60]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3r60]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_subtilis Bacillus subtilis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R60 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3R60 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3r60]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R60 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3R60 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=PGO:S-1,2-PROPANEDIOL'>PGO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=PGO:S-1,2-PROPANEDIOL'>PGO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3r61\|3r61]], [[2f5d\|2f5d]], [[2ev6\|2ev6]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3r61\|3r61]], [[2f5d\|2f5d]], [[2ev6\|2ev6]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Bacillus subtilis, BSU24520, mntR, yqhN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus ~~subtilis~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Bacillus subtilis, BSU24520, mntR, yqhN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Bacillus globigii" Migula 1900])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3r60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r60 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3r60 RCSB], [http://www.ebi.ac.uk/pdbsum/3r60 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3r60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r60 OCA], [http://pdbe.org/3r60 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3r60 RCSB], [http://www.ebi.ac.uk/pdbsum/3r60 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3r60 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3r60" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus ~~subtilis~~]]	+	[[Category: Bacillus globigii migula 1900]]
	[[Category: Brophy, M B]]		[[Category: Brophy, M B]]
	[[Category: Glasfeld, A]]		[[Category: Glasfeld, A]]

OCA at 12:41, 25 December 2014

OCA — Thu, 25 Dec 2014 12:41:25 GMT

←Older revision		Revision as of 12:41, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3r60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r60 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3r60 RCSB], [http://www.ebi.ac.uk/pdbsum/3r60 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3r60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r60 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3r60 RCSB], [http://www.ebi.ac.uk/pdbsum/3r60 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MNTR_BACSU MNTR_BACSU]] Central regulator of manganese homeostasis. In the presence of manganese, it mediates repression of the manganese transporter MntH; under low manganese conditions, it activates the transcription of the mntABCD operon.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 12:24, 9 December 2014

OCA — Tue, 09 Dec 2014 12:24:12 GMT

←Older revision		Revision as of 12:24, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3r60.~~png~~\|~~left~~\|~~200px~~]]	+	==Structure of the MntR Fe2+ Complex==
		+	<StructureSection load='3r60' size='340' side='right' caption='[[3r60]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3r60]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R60 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3R60 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=PGO:S-1,2-PROPANEDIOL'>PGO</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3r61\|3r61]], [[2f5d\|2f5d]], [[2ev6\|2ev6]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Bacillus subtilis, BSU24520, mntR, yqhN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3r60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r60 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3r60 RCSB], [http://www.ebi.ac.uk/pdbsum/3r60 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The manganese transport regulator (MntR) represses the expression of genes involved in manganese uptake in Bacillus subtilis. It selectively responds to Mn(2+) and Cd(2+) over other divalent metal cations, including Fe(2+), Co(2+), and Zn(2+). Previous work has shown that MntR forms binuclear complexes with Mn(2+) or Cd(2+) at two binding sites, labeled A and C, that are separated by 4.4 A. Zinc activates MntR poorly and binds only to the A site, forming a mononuclear complex. The difference in metal binding stoichiometry suggested a mechanism for selectivity in MntR. Larger metal cations are strongly activating because they can form the binuclear complex, while smaller metal ions cannot bind with the geometry needed to fully occupy both metal binding sites. To investigate this hypothesis, structures of MntR in complex with two other noncognate metal ions, Fe(2+) and Co(2+), have been determined. Each metal forms a mononuclear complex with MntR with the metal ion bound in the A site, supporting the conclusions drawn from the Zn(2+) complex. Additionally, we investigated two site-specific mutants of MntR, E11K and H77A, that contain substitutions of metal binding residues in the A site. While metal binding in each mutant is significantly altered relative to that of wild-type MntR, both mutants retain activity and selectivity for Mn(2+) in vitro and in vivo. That observation, coupled with previous studies, suggests that the A and C sites both contribute to the selectivity of MntR.

-	~~{{STRUCTURE_3r60\| PDB=3r60 \| SCENE= }}~~	+	Roles of the A and C Sites in the Manganese-Specific Activation of MntR.,McGuire AM, Cuthbert BJ, Ma Z, Grauer-Gray KD, Brunjes Brophy M, Spear KA, Soonsanga S, Kliegman JI, Griner SL, Helmann JD, Glasfeld A Biochemistry. 2013 Jan 29;52(4):701-13. doi: 10.1021/bi301550t. Epub 2013 Jan 17. PMID:23298157<ref>PMID:23298157</ref>

-	~~===Structure~~ of the ~~MntR Fe2+ Complex===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_23298157}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[3r60]] is a 2 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R60 OCA].~~	+	</StructureSection>
	[[Category: Bacillus subtilis]]		[[Category: Bacillus subtilis]]
-	[[Category: Brophy, M B.]]	+	[[Category: Brophy, M B]]
-	[[Category: Glasfeld, A.]]	+	[[Category: Glasfeld, A]]
-	[[Category: Griner, S L.]]	+	[[Category: Griner, S L]]
-	[[Category: Kliegman, J I.]]	+	[[Category: Kliegman, J I]]
-	[[Category: Nix, J C.]]	+	[[Category: Nix, J C]]
	[[Category: Transcription regulator]]		[[Category: Transcription regulator]]
	[[Category: Winged helix-turn-helix]]		[[Category: Winged helix-turn-helix]]

OCA at 12:52, 6 February 2013

OCA — Wed, 06 Feb 2013 12:52:20 GMT

←Older revision		Revision as of 12:52, 6 February 2013
Line 1:		Line 1:
-	[[Image:3r60.~~jpg~~\|left\|200px]]	+	[[Image:3r60.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3r60", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3r60\| PDB=3r60 \| SCENE= }}		{{STRUCTURE_3r60\| PDB=3r60 \| SCENE= }}

	===Structure of the MntR Fe2+ Complex===		===Structure of the MntR Fe2+ Complex===

		+	{{ABSTRACT_PUBMED_23298157}}

	==About this Structure==		==About this Structure==

OCA at 07:06, 11 April 2012

OCA — Wed, 11 Apr 2012 07:06:31 GMT

←Older revision		Revision as of 07:06, 11 April 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3r60.jpg\|left\|200px]]

-	The ~~entry 3r60~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3r60", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3r60\| PDB=3r60 \| SCENE= }}

-	~~Authors: Glasfeld, A., Brophy, M.B., Kliegman, J.I., Griner, S.L., Nix, J.C.~~	+	===Structure of the MntR Fe2+ Complex===

-	~~Description:~~ Structure ~~of the MntR Fe2+ Complex~~	+
		+	==About this Structure==
		+	[[3r60]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R60 OCA].
		+	[[Category: Bacillus subtilis]]
		+	[[Category: Brophy, M B.]]
		+	[[Category: Glasfeld, A.]]
		+	[[Category: Griner, S L.]]
		+	[[Category: Kliegman, J I.]]
		+	[[Category: Nix, J C.]]
		+	[[Category: Transcription regulator]]
		+	[[Category: Winged helix-turn-helix]]

OCA at 07:50, 2 November 2011

OCA — Wed, 02 Nov 2011 07:50:11 GMT

←Older revision		Revision as of 07:50, 2 November 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3r60 is ON HOLD	+	The entry 3r60 is ON HOLD until Paper Publication

	Authors: Glasfeld, A., Brophy, M.B., Kliegman, J.I., Griner, S.L., Nix, J.C.		Authors: Glasfeld, A., Brophy, M.B., Kliegman, J.I., Griner, S.L., Nix, J.C.

	Description: Structure of the MntR Fe2+ Complex		Description: Structure of the MntR Fe2+ Complex

OCA at 06:02, 5 October 2011

OCA — Wed, 05 Oct 2011 06:02:13 GMT

←Older revision		Revision as of 06:02, 5 October 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3r60 is ON HOLD ~~until Paper Publication~~	+	The entry 3r60 is ON HOLD

	Authors: Glasfeld, A., Brophy, M.B., Kliegman, J.I., Griner, S.L., Nix, J.C.		Authors: Glasfeld, A., Brophy, M.B., Kliegman, J.I., Griner, S.L., Nix, J.C.

	Description: Structure of the MntR Fe2+ Complex		Description: Structure of the MntR Fe2+ Complex

OCA at 08:39, 6 April 2011

OCA — Wed, 06 Apr 2011 08:39:10 GMT

←Older revision		Revision as of 08:39, 6 April 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3r60 is ON HOLD	+	The entry 3r60 is ON HOLD until Paper Publication

	Authors: Glasfeld, A., Brophy, M.B., Kliegman, J.I., Griner, S.L., Nix, J.C.		Authors: Glasfeld, A., Brophy, M.B., Kliegman, J.I., Griner, S.L., Nix, J.C.

	Description: Structure of the MntR Fe2+ Complex		Description: Structure of the MntR Fe2+ Complex