3rb7 - Revision history

OCA at 10:53, 21 February 2024

OCA — Wed, 21 Feb 2024 10:53:35 GMT

←Older revision		Revision as of 10:53, 21 February 2024
Line 3:		Line 3:
	<StructureSection load='3rb7' size='340' side='right'caption='[[3rb7]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='3rb7' size='340' side='right'caption='[[3rb7]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rb7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Drome Drome~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RB7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RB7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rb7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RB7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RB7 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.9Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[3rb5\|3rb5]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">Calx, NCX, CG5685 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rb7 OCA], [https://pdbe.org/3rb7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rb7 RCSB], [https://www.ebi.ac.uk/pdbsum/3rb7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rb7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rb7 OCA], [https://pdbe.org/3rb7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rb7 RCSB], [https://www.ebi.ac.uk/pdbsum/3rb7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rb7 ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q9VDG5_DROME Q9VDG5_DROME]
-	The Na(+)/Ca(2+) exchanger CALX promotes Ca(2+) efflux in Drosophila sensory neuronal cells to facilitate light-mediated Ca(2+) homeostasis. CALX activity is negatively regulated by specific Ca(2+) interaction within its two intracellular Ca(2+) regulatory domains CBD1 and CBD2, yet how the Ca(2+) binding is converted to molecular motion to operate the exchanger is unknown. Here, we report crystal structures of the entire Ca(2+) regulatory domain CBD12 from two alternative splicing isoforms, CALX 1.1 and 1.2, exhibiting distinct regulatory Ca(2+) dependency. The structures show an open V-shaped conformation with four Ca(2+) ions bound on the CBD domain interface, confirmed by LRET analysis. The structures together with Ca(2+)-binding analysis support that the Ca(2+) inhibition of CALX is achieved by interdomain conformational changes induced by Ca(2+) binding at CBD1. The conformational difference between the two isoforms also indicates that alternative splicing adjusts the interdomain orientation angle to modify the Ca(2+) regulatory property of the exchangers.	+
-		+
-	Structural Basis of the Ca(2+) Inhibitory Mechanism of Drosophila Na(+)/Ca(2+) Exchanger CALX and Its Modification by Alternative Splicing.,Wu M, Tong S, Gonzalez J, Jayaraman V, Spudich JL, Zheng L Structure. 2011 Oct 12;19(10):~~1509-17. PMID:22000518<ref>PMID:22000518<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3rb7" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Drome~~]]	+	[[Category: Drosophila melanogaster]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Wu, M]]	+	[[Category: Wu M]]
-	[[Category: Zheng, L]]	+	[[Category: Zheng L]]
-	~~[[Category: Calcium binding]]~~	+
-	~~[[Category: Calcium binding domain]]~~	+
-	~~[[Category: Metal binding protein~~]]	+

OCA at 05:56, 15 June 2022

OCA — Wed, 15 Jun 2022 05:56:20 GMT

←Older revision		Revision as of 05:56, 15 June 2022
Line 1:		Line 1:

	==Crystal structure of CBD12 from CALX1.2==		==Crystal structure of CBD12 from CALX1.2==
-	<StructureSection load='3rb7' size='340' side='right' caption='[[3rb7]], [[Resolution\|resolution]] 2.90Å' scene=''>	+	<StructureSection load='3rb7' size='340' side='right'caption='[[3rb7]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rb7]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RB7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3RB7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rb7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RB7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RB7 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rb5\|3rb5]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3rb5\|3rb5]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Calx, NCX, CG5685 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Calx, NCX, CG5685 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3rb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rb7 OCA], [~~http~~://pdbe.org/3rb7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3rb7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3rb7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3rb7 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rb7 OCA], [https://pdbe.org/3rb7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rb7 RCSB], [https://www.ebi.ac.uk/pdbsum/3rb7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rb7 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Drome]]		[[Category: Drome]]
		+	[[Category: Large Structures]]
	[[Category: Wu, M]]		[[Category: Wu, M]]
	[[Category: Zheng, L]]		[[Category: Zheng, L]]

OCA at 19:08, 11 August 2016

OCA — Thu, 11 Aug 2016 19:08:44 GMT

←Older revision		Revision as of 19:08, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of CBD12 from CALX1.2==		==Crystal structure of CBD12 from CALX1.2==
	<StructureSection load='3rb7' size='340' side='right' caption='[[3rb7]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='3rb7' size='340' side='right' caption='[[3rb7]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rb7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Drosophila_melanogaster Drosophila melanogaster~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RB7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RB7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rb7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RB7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RB7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rb5\|3rb5]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rb5\|3rb5]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Calx, NCX, CG5685 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 ~~Drosophila melanogaster~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Calx, NCX, CG5685 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rb7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rb7 RCSB], [http://www.ebi.ac.uk/pdbsum/3rb7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rb7 OCA], [http://pdbe.org/3rb7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3rb7 RCSB], [http://www.ebi.ac.uk/pdbsum/3rb7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3rb7 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3rb7" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Drosophila melanogaster~~]]	+	[[Category: Drome]]
	[[Category: Wu, M]]		[[Category: Wu, M]]
	[[Category: Zheng, L]]		[[Category: Zheng, L]]

OCA at 10:45, 4 January 2015

OCA — Sun, 04 Jan 2015 10:45:34 GMT

←Older revision		Revision as of 10:45, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3rb7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RB7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RB7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3rb7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RB7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RB7 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rb5\|3rb5]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rb5\|3rb5]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Calx, NCX, CG5685 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Calx, NCX, CG5685 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rb7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rb7 RCSB], [http://www.ebi.ac.uk/pdbsum/3rb7 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rb7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rb7 RCSB], [http://www.ebi.ac.uk/pdbsum/3rb7 PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 14:
	Structural Basis of the Ca(2+) Inhibitory Mechanism of Drosophila Na(+)/Ca(2+) Exchanger CALX and Its Modification by Alternative Splicing.,Wu M, Tong S, Gonzalez J, Jayaraman V, Spudich JL, Zheng L Structure. 2011 Oct 12;19(10):1509-17. PMID:22000518<ref>PMID:22000518</ref>		Structural Basis of the Ca(2+) Inhibitory Mechanism of Drosophila Na(+)/Ca(2+) Exchanger CALX and Its Modification by Alternative Splicing.,Wu M, Tong S, Gonzalez J, Jayaraman V, Spudich JL, Zheng L Structure. 2011 Oct 12;19(10):1509-17. PMID:22000518<ref>PMID:22000518</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Drosophila melanogaster]]		[[Category: Drosophila melanogaster]]
-	[[Category: Wu, M.]]	+	[[Category: Wu, M]]
-	[[Category: Zheng, L.]]	+	[[Category: Zheng, L]]
	[[Category: Calcium binding]]		[[Category: Calcium binding]]
	[[Category: Calcium binding domain]]		[[Category: Calcium binding domain]]
	[[Category: Metal binding protein]]		[[Category: Metal binding protein]]

OCA at 04:41, 5 June 2014

OCA — Thu, 05 Jun 2014 04:41:17 GMT

←Older revision		Revision as of 04:41, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3rb7.~~jpg~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of CBD12 from CALX1.2==
		+	<StructureSection load='3rb7' size='340' side='right' caption='[[3rb7]], [[Resolution\|resolution]] 2.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3rb7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RB7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RB7 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rb5\|3rb5]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Calx, NCX, CG5685 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rb7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rb7 RCSB], [http://www.ebi.ac.uk/pdbsum/3rb7 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Na(+)/Ca(2+) exchanger CALX promotes Ca(2+) efflux in Drosophila sensory neuronal cells to facilitate light-mediated Ca(2+) homeostasis. CALX activity is negatively regulated by specific Ca(2+) interaction within its two intracellular Ca(2+) regulatory domains CBD1 and CBD2, yet how the Ca(2+) binding is converted to molecular motion to operate the exchanger is unknown. Here, we report crystal structures of the entire Ca(2+) regulatory domain CBD12 from two alternative splicing isoforms, CALX 1.1 and 1.2, exhibiting distinct regulatory Ca(2+) dependency. The structures show an open V-shaped conformation with four Ca(2+) ions bound on the CBD domain interface, confirmed by LRET analysis. The structures together with Ca(2+)-binding analysis support that the Ca(2+) inhibition of CALX is achieved by interdomain conformational changes induced by Ca(2+) binding at CBD1. The conformational difference between the two isoforms also indicates that alternative splicing adjusts the interdomain orientation angle to modify the Ca(2+) regulatory property of the exchangers.

-	~~<!--~~	+	Structural Basis of the Ca(2+) Inhibitory Mechanism of Drosophila Na(+)/Ca(2+) Exchanger CALX and Its Modification by Alternative Splicing.,Wu M, Tong S, Gonzalez J, Jayaraman V, Spudich JL, Zheng L Structure. 2011 Oct 12;19(10):1509-17. PMID:22000518<ref>PMID:22000518</ref>
-	~~The line below this paragraph, containing "STRUCTURE_3rb7", creates~~ the ~~"Structure Box" on the page.~~	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter~~ (~~which sets the initial scene displayed when the page is loaded~~),	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3rb7\| PDB=3rb7 \| SCENE= }}~~	+

-	~~===Crystal structure~~ of ~~CBD12 from CALX1.2===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22000518}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22000518 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_22000518}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3rb7]] is a 4 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RB7 OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022000518<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Drosophila melanogaster]]		[[Category: Drosophila melanogaster]]
	[[Category: Wu, M.]]		[[Category: Wu, M.]]

OCA at 08:19, 2 November 2011

OCA — Wed, 02 Nov 2011 08:19:15 GMT

←Older revision		Revision as of 08:19, 2 November 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3rb7.jpg\|left\|200px]]

-	The ~~entry 3rb7~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3rb7", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3rb7\| PDB=3rb7 \| SCENE= }}

-	~~Authors: Wu, M., Zheng, L~~.	+	===Crystal structure of CBD12 from CALX1.2===

-	~~Description~~: ~~Crystal~~ structure ~~of CBD12~~ from ~~CALX1~~.2	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22000518}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22000518 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22000518}}
		+
		+	==About this Structure==
		+	[[3rb7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RB7 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022000518</ref><references group="xtra"/>
		+	[[Category: Drosophila melanogaster]]
		+	[[Category: Wu, M.]]
		+	[[Category: Zheng, L.]]
		+	[[Category: Calcium binding]]
		+	[[Category: Calcium binding domain]]
		+	[[Category: Metal binding protein]]

OCA at 10:19, 21 April 2011

OCA — Thu, 21 Apr 2011 10:19:58 GMT

←Older revision		Revision as of 10:19, 21 April 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3rb7 is ON HOLD	+	The entry 3rb7 is ON HOLD until Paper Publication

	Authors: Wu, M., Zheng, L.		Authors: Wu, M., Zheng, L.

	Description: Crystal structure of CBD12 from CALX1.2		Description: Crystal structure of CBD12 from CALX1.2

OCA at 04:57, 13 April 2011

OCA — Wed, 13 Apr 2011 04:57:56 GMT

←Older revision		Revision as of 04:57, 13 April 2011
Line 3:		Line 3:
	The entry 3rb7 is ON HOLD		The entry 3rb7 is ON HOLD

-	Authors: Wu, M, Zheng, L	+	Authors: Wu, M., Zheng, L.

	Description: Crystal structure of CBD12 from CALX1.2		Description: Crystal structure of CBD12 from CALX1.2

OCA: Protected "3rb7" [edit=sysop:move=sysop]

OCA — Wed, 06 Apr 2011 08:42:20 GMT

Protected "3rb7" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:42, 6 April 2011

OCA: New page: '''Unreleased structure''' The entry 3rb7 is ON HOLD Authors: Wu, M, Zheng, L Description: Crystal structure of CBD12 from CALX1.2

OCA — Wed, 06 Apr 2011 08:42:19 GMT

New page: '''Unreleased structure''' The entry 3rb7 is ON HOLD Authors: Wu, M, Zheng, L Description: Crystal structure of CBD12 from CALX1.2

New page

'''Unreleased structure'''

The entry 3rb7 is ON HOLD

Authors: Wu, M, Zheng, L

Description: Crystal structure of CBD12 from CALX1.2