3rgd - Revision history

OCA at 12:22, 14 March 2024

OCA — Thu, 14 Mar 2024 12:22:36 GMT

←Older revision		Revision as of 12:22, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3rgd' size='340' side='right'caption='[[3rgd]], [[Resolution\|resolution]] 2.89Å' scene=''>		<StructureSection load='3rgd' size='340' side='right'caption='[[3rgd]], [[Resolution\|resolution]] 2.89Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rgd]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/~~American_bullfrog American bullfrog~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RGD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RGD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rgd]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Lithobates_catesbeianus Lithobates catesbeianus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RGD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RGD FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.89Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[3rbc\|3rbc]], [[3re7\|3re7]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Ferroxidase Ferroxidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.3.1 1.16.3.1]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rgd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rgd OCA], [https://pdbe.org/3rgd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rgd RCSB], [https://www.ebi.ac.uk/pdbsum/3rgd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rgd ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rgd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rgd OCA], [https://pdbe.org/3rgd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rgd RCSB], [https://www.ebi.ac.uk/pdbsum/3rgd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rgd ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/FRI2_LITCT FRI2_LITCT]] Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.	+	[https://www.uniprot.org/uniprot/FRI2_LITCT FRI2_LITCT] Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The first step of iron biomineralization mediated by ferritin is the oxidation at the ferroxidase active site of two ferrous ions to a diferric oxo/hydroxo species. Metal-loaded ferritin crystals obtained by soaking crystals of frog ferritin in FeSO(4) and CuSO(4) solutions followed by flash freezing provided X-ray crystal structures of the tripositive iron and bipositive copper adducts at 2.7 and 2.8 A resolution, respectively. At variance with the already available structures, the crystal form used in this study contains 24 independent subunits in the asymmetric unit permitting comparison between them. For the first time, the diferric species at the ferroxidase site is identified in ferritins from higher eukaryotes. Anomalous difference Fourier maps for crystals (iron crystal 1) obtained after long soaking times in FeSO(4) solution invariantly showed diferric species with a Fe-Fe average distance of 3.1 +/- 0.1 A, strongly indicative of the presence of a mu-oxo/hydroxo bridge between the irons; protein ligands for each iron ion (Fe1 and Fe2) were also unequivocally identified and found to be the same in all subunits. For copper bound ferritin, dicopper(II) centers are also observed. While copper at site 1 is essentially in the same position and has the same coordination environment as Fe1, copper at site 2 is displaced toward His54, now acting as a ligand; this results in an increased intermetal distance (4.3 +/- 0.4 A). His54 coordination and longer metal-metal distances might represent peculiar features of divalent cations at the ferroxidase site. This oxidation-dependent structural information may provide key features for the mechanistic pathway in ferritins from higher eukaryotes that drive uptake of bivalent cation and release of ferric products at the catalytic site. This mechanism is supported by the X-ray picture obtained after only 1 min of soaking in FeSO(4) solutions (iron crystal 2) which reasonably contain the metal at different oxidation states. Here two different di-iron species are trapped in the active site, with intermetal distances corresponding to those of the ferric dimer in crystal 1 and of the dicopper centers and corresponding rearrangement of the His54 side chain.	+
-		+
-	Structural insights into the ferroxidase site of ferritins from higher eukaryotes.,Bertini I, Lalli D, Mangani S, Pozzi C, Rosa C, Theil EC, Turano P J Am Chem Soc. 2012 Apr 11;134(14):6169-76. doi: 10.1021/ja210084n. Epub 2012 Mar, 28. PMID:22424302<ref>PMID:22424302</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3rgd" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Ferritin 3D structures\|Ferritin 3D structures]]		*[[Ferritin 3D structures\|Ferritin 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: American bullfrog]]
-	[[Category: Ferroxidase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bertini, I]]	+	[[Category: Lithobates catesbeianus]]
-	[[Category: Lalli, D]]	+	[[Category: Bertini I]]
-	[[Category: Mangani, S]]	+	[[Category: Lalli D]]
-	[[Category: Pozzi, C]]	+	[[Category: Mangani S]]
-	[[Category: Rosa, C]]	+	[[Category: Pozzi C]]
-	[[Category: Theil~~, E C~~]]	+	[[Category: Rosa C]]
-	[[Category: Turano, P]]	+	[[Category: Theil EC]]
-	~~[[Category: Eukaryotic ferritin]]~~	+	[[Category: Turano P]]
-	~~[[Category: Four-helix bundle]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 06:02, 15 June 2022

OCA — Wed, 15 Jun 2022 06:02:15 GMT

←Older revision		Revision as of 06:02, 15 June 2022
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	<StructureSection load='3rgd' size='340' side='right'caption='[[3rgd]], [[Resolution\|resolution]] 2.89Å' scene=''>		<StructureSection load='3rgd' size='340' side='right'caption='[[3rgd]], [[Resolution\|resolution]] 2.89Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rgd]] is a 24 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/American_bullfrog American bullfrog]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RGD OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3RGD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rgd]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/American_bullfrog American bullfrog]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RGD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RGD FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rbc\|3rbc]], [[3re7\|3re7]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3rbc\|3rbc]], [[3re7\|3re7]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Ferroxidase Ferroxidase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.3.1 1.16.3.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Ferroxidase Ferroxidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.3.1 1.16.3.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3rgd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rgd OCA], [~~http~~://pdbe.org/3rgd PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3rgd RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3rgd PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3rgd ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rgd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rgd OCA], [https://pdbe.org/3rgd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rgd RCSB], [https://www.ebi.ac.uk/pdbsum/3rgd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rgd ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/FRI2_LITCT FRI2_LITCT]] Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.	+	[[https://www.uniprot.org/uniprot/FRI2_LITCT FRI2_LITCT]] Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 17:44, 14 August 2019

OCA — Wed, 14 Aug 2019 17:44:21 GMT

←Older revision		Revision as of 17:44, 14 August 2019
Line 1:		Line 1:

	==Iron loaded frog M ferritin. Short soaking time==		==Iron loaded frog M ferritin. Short soaking time==
-	<StructureSection load='3rgd' size='340' side='right' caption='[[3rgd]], [[Resolution\|resolution]] 2.89Å' scene=''>	+	<StructureSection load='3rgd' size='340' side='right'caption='[[3rgd]], [[Resolution\|resolution]] 2.89Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3rgd]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/American_bullfrog American bullfrog]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RGD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RGD FirstGlance]. <br>		<table><tr><td colspan='2'>[[3rgd]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/American_bullfrog American bullfrog]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RGD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RGD FirstGlance]. <br>
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 3rgd" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3rgd" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ferritin 3D structures\|Ferritin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 26:		Line 29:
	[[Category: American bullfrog]]		[[Category: American bullfrog]]
	[[Category: Ferroxidase]]		[[Category: Ferroxidase]]
		+	[[Category: Large Structures]]
	[[Category: Bertini, I]]		[[Category: Bertini, I]]
	[[Category: Lalli, D]]		[[Category: Lalli, D]]

OCA at 09:25, 8 November 2017

OCA — Wed, 08 Nov 2017 09:25:40 GMT

←Older revision		Revision as of 09:25, 8 November 2017
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 3rgd" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3rgd" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Ferritin\|Ferritin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 08:34, 4 August 2016

OCA — Thu, 04 Aug 2016 08:34:41 GMT

←Older revision		Revision as of 08:34, 4 August 2016
Line 1:		Line 1:
		+
	==Iron loaded frog M ferritin. Short soaking time==		==Iron loaded frog M ferritin. Short soaking time==
	<StructureSection load='3rgd' size='340' side='right' caption='[[3rgd]], [[Resolution\|resolution]] 2.89Å' scene=''>		<StructureSection load='3rgd' size='340' side='right' caption='[[3rgd]], [[Resolution\|resolution]] 2.89Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rgd]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Rana_catesbeiana Rana catesbeiana~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RGD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RGD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rgd]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/American_bullfrog American bullfrog]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RGD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RGD FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rbc\|3rbc]], [[3re7\|3re7]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rbc\|3rbc]], [[3re7\|3re7]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferroxidase Ferroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.3.1 1.16.3.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferroxidase Ferroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.3.1 1.16.3.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rgd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rgd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rgd RCSB], [http://www.ebi.ac.uk/pdbsum/3rgd PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rgd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rgd OCA], [http://pdbe.org/3rgd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3rgd RCSB], [http://www.ebi.ac.uk/pdbsum/3rgd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3rgd ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3rgd" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: American bullfrog]]
	[[Category: Ferroxidase]]		[[Category: Ferroxidase]]
-	[[Category: Rana catesbeiana]]
	[[Category: Bertini, I]]		[[Category: Bertini, I]]
	[[Category: Lalli, D]]		[[Category: Lalli, D]]

OCA at 07:22, 25 December 2014

OCA — Thu, 25 Dec 2014 07:22:49 GMT

←Older revision		Revision as of 07:22, 25 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3rgd\| PDB=3rgd \| SCENE= }}~~	+	==Iron loaded frog M ferritin. Short soaking time==
-	===Iron loaded frog M ferritin. Short soaking time===	+	<StructureSection load='3rgd' size='340' side='right' caption='[[3rgd]], [[Resolution\|resolution]] 2.89Å' scene=''>
-	~~{{ABSTRACT_PUBMED_22424302}}~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[3rgd]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Rana_catesbeiana Rana catesbeiana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RGD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RGD FirstGlance]. <br>
-	==Function==	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rbc\|3rbc]], [[3re7\|3re7]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferroxidase Ferroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.3.1 1.16.3.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rgd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rgd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rgd RCSB], [http://www.ebi.ac.uk/pdbsum/3rgd PDBsum]</span></td></tr>
		+	</table>
		+	== Function ==
	[[http://www.uniprot.org/uniprot/FRI2_LITCT FRI2_LITCT]] Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.		[[http://www.uniprot.org/uniprot/FRI2_LITCT FRI2_LITCT]] Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The first step of iron biomineralization mediated by ferritin is the oxidation at the ferroxidase active site of two ferrous ions to a diferric oxo/hydroxo species. Metal-loaded ferritin crystals obtained by soaking crystals of frog ferritin in FeSO(4) and CuSO(4) solutions followed by flash freezing provided X-ray crystal structures of the tripositive iron and bipositive copper adducts at 2.7 and 2.8 A resolution, respectively. At variance with the already available structures, the crystal form used in this study contains 24 independent subunits in the asymmetric unit permitting comparison between them. For the first time, the diferric species at the ferroxidase site is identified in ferritins from higher eukaryotes. Anomalous difference Fourier maps for crystals (iron crystal 1) obtained after long soaking times in FeSO(4) solution invariantly showed diferric species with a Fe-Fe average distance of 3.1 +/- 0.1 A, strongly indicative of the presence of a mu-oxo/hydroxo bridge between the irons; protein ligands for each iron ion (Fe1 and Fe2) were also unequivocally identified and found to be the same in all subunits. For copper bound ferritin, dicopper(II) centers are also observed. While copper at site 1 is essentially in the same position and has the same coordination environment as Fe1, copper at site 2 is displaced toward His54, now acting as a ligand; this results in an increased intermetal distance (4.3 +/- 0.4 A). His54 coordination and longer metal-metal distances might represent peculiar features of divalent cations at the ferroxidase site. This oxidation-dependent structural information may provide key features for the mechanistic pathway in ferritins from higher eukaryotes that drive uptake of bivalent cation and release of ferric products at the catalytic site. This mechanism is supported by the X-ray picture obtained after only 1 min of soaking in FeSO(4) solutions (iron crystal 2) which reasonably contain the metal at different oxidation states. Here two different di-iron species are trapped in the active site, with intermetal distances corresponding to those of the ferric dimer in crystal 1 and of the dicopper centers and corresponding rearrangement of the His54 side chain.

-	~~==About this Structure==~~	+	Structural insights into the ferroxidase site of ferritins from higher eukaryotes.,Bertini I, Lalli D, Mangani S, Pozzi C, Rosa C, Theil EC, Turano P J Am Chem Soc. 2012 Apr 11;134(14):6169-76. doi: 10.1021/ja210084n. Epub 2012 Mar, 28. PMID:22424302<ref>PMID:22424302</ref>
-	~~[[3rgd]] is a 24 chain structure with sequence~~ from ~~[http~~:~~//en~~.~~wikipedia~~.~~org~~/~~wiki/Rana_catesbeiana Rana catesbeiana]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=3RGD OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[Ferritin\|Ferritin]]		*[[Ferritin\|Ferritin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:022424302</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Ferroxidase]]		[[Category: Ferroxidase]]
	[[Category: Rana catesbeiana]]		[[Category: Rana catesbeiana]]
-	[[Category: Bertini, I.]]	+	[[Category: Bertini, I]]
-	[[Category: Lalli, D.]]	+	[[Category: Lalli, D]]
-	[[Category: Mangani, S.]]	+	[[Category: Mangani, S]]
-	[[Category: Pozzi, C.]]	+	[[Category: Pozzi, C]]
-	[[Category: Rosa, C.]]	+	[[Category: Rosa, C]]
-	[[Category: Theil, E C.]]	+	[[Category: Theil, E C]]
-	[[Category: Turano, P.]]	+	[[Category: Turano, P]]
	[[Category: Eukaryotic ferritin]]		[[Category: Eukaryotic ferritin]]
-	[[Category: Ferroxidase]]
	[[Category: Four-helix bundle]]		[[Category: Four-helix bundle]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 11:08, 24 July 2013

OCA — Wed, 24 Jul 2013 11:08:44 GMT

←Older revision		Revision as of 11:08, 24 July 2013
Line 1:		Line 1:
-	[[Image:3rgd.png\|left\|200px]]
-
	{{STRUCTURE_3rgd\| PDB=3rgd \| SCENE= }}		{{STRUCTURE_3rgd\| PDB=3rgd \| SCENE= }}
-
	===Iron loaded frog M ferritin. Short soaking time===		===Iron loaded frog M ferritin. Short soaking time===
		+	{{ABSTRACT_PUBMED_22424302}}

		+	==Function==
		+	[[http://www.uniprot.org/uniprot/FRI2_LITCT FRI2_LITCT]] Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.

	==About this Structure==		==About this Structure==
Line 11:		Line 11:
	==See Also==		==See Also==
	*[[Ferritin\|Ferritin]]		*[[Ferritin\|Ferritin]]
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022424302</ref><references group="xtra"/><references/>
	[[Category: Ferroxidase]]		[[Category: Ferroxidase]]
	[[Category: Rana catesbeiana]]		[[Category: Rana catesbeiana]]

OCA at 14:08, 20 October 2012

OCA — Sat, 20 Oct 2012 14:08:43 GMT

←Older revision		Revision as of 14:08, 20 October 2012
Line 7:		Line 7:

	==About this Structure==		==About this Structure==
-	[[3rgd]] is a 24 chain structure ~~of [[Ferritin]]~~ with sequence from [http://en.wikipedia.org/wiki/Rana_catesbeiana Rana catesbeiana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RGD OCA].	+	[[3rgd]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Rana_catesbeiana Rana catesbeiana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RGD OCA].

	==See Also==		==See Also==

OCA at 14:11, 26 July 2012

OCA — Thu, 26 Jul 2012 14:11:53 GMT

←Older revision		Revision as of 14:11, 26 July 2012
Line 1:		Line 1:
-	[[Image:3rgd.~~jpg~~\|left\|200px]]	+	[[Image:3rgd.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3rgd", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3rgd\| PDB=3rgd \| SCENE= }}		{{STRUCTURE_3rgd\| PDB=3rgd \| SCENE= }}

Line 13:		Line 7:

	==About this Structure==		==About this Structure==
-	[[3rgd]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Rana_catesbeiana Rana catesbeiana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RGD OCA].	+	[[3rgd]] is a 24 chain structure of [[Ferritin]] with sequence from [http://en.wikipedia.org/wiki/Rana_catesbeiana Rana catesbeiana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RGD OCA].
		+
		+	==See Also==
		+	*[[Ferritin\|Ferritin]]
	[[Category: Ferroxidase]]		[[Category: Ferroxidase]]
	[[Category: Rana catesbeiana]]		[[Category: Rana catesbeiana]]

OCA at 07:06, 11 April 2012

OCA — Wed, 11 Apr 2012 07:06:56 GMT

←Older revision		Revision as of 07:06, 11 April 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3rgd.jpg\|left\|200px]]

-	The ~~entry 3rgd~~ is ~~ON HOLD~~ ~~until Apr 08 2013~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3rgd", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3rgd\| PDB=3rgd \| SCENE= }}

-	~~Authors: Bertini, I., Lalli, D., Mangani, S., Pozzi, C., Rosa, C., Theil, E.C., Turano, P~~.	+	===Iron loaded frog M ferritin. Short soaking time===

-	~~Description~~: ~~Iron loaded frog M ferritin~~. ~~Short soaking time~~	+
		+	==About this Structure==
		+	[[3rgd]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Rana_catesbeiana Rana catesbeiana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RGD OCA].
		+	[[Category: Ferroxidase]]
		+	[[Category: Rana catesbeiana]]
		+	[[Category: Bertini, I.]]
		+	[[Category: Lalli, D.]]
		+	[[Category: Mangani, S.]]
		+	[[Category: Pozzi, C.]]
		+	[[Category: Rosa, C.]]
		+	[[Category: Theil, E C.]]
		+	[[Category: Turano, P.]]
		+	[[Category: Eukaryotic ferritin]]
		+	[[Category: Ferroxidase]]
		+	[[Category: Four-helix bundle]]
		+	[[Category: Oxidoreductase]]