3rhl - Revision history

OCA at 12:24, 14 March 2024

OCA — Thu, 14 Mar 2024 12:24:13 GMT

←Older revision		Revision as of 12:24, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3rhl' size='340' side='right'caption='[[3rhl]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3rhl' size='340' side='right'caption='[[3rhl]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rhl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Buffalo_rat Buffalo rat~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RHL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RHL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rhl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RHL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RHL FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE~~-~~ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[3rhj\|3rhj]], [[3rhm\|3rhm]], [[3rho\|3rho]], [[3rhp\|3rhp]], [[3rhq\|3rhq]], [[3rhr\|3rhr]]~~</~~div~~><~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<~~tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">Aldh1l1, Fthfd ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rhl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rhl OCA], [https://pdbe.org/3rhl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rhl RCSB], [https://www.ebi.ac.uk/pdbsum/3rhl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rhl ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rhl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rhl OCA], [https://pdbe.org/3rhl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rhl RCSB], [https://www.ebi.ac.uk/pdbsum/3rhl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rhl ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/AL1L1_RAT AL1L1_RAT]
-	The C-terminal domain (C(t)-FDH) of 10-formyltetrahydrofolate dehydrogenase (FDH, ALDH1L1) is an NADP(+)-dependent oxidoreductase and a structural and functional homolog of aldehyde dehydrogenases. Here we report the crystal structures of several C(t)-FDH mutants in which two essential catalytic residues adjacent to the nicotinamide ring of bound NADP(+), Cys-707 and Glu-673, were replaced separately or simultaneously. The replacement of the glutamate with an alanine causes irreversible binding of the coenzyme without any noticeable conformational changes in the vicinity of the nicotinamide ring. Additional replacement of cysteine 707 with an alanine (E673A/C707A double mutant) did not affect this irreversible binding indicating that the lack of the glutamate is solely responsible for the enhanced interaction between the enzyme and the coenzyme. The substitution of the cysteine with an alanine did not affect binding of NADP(+) but resulted in the enzyme lacking the ability to differentiate between the oxidized and reduced coenzyme: ~~unlike the wild-type C(t)-FDH~~/NADPH complex, in the C707A mutant the position of NADPH is identical to the position of NADP(+) with the nicotinamide ring well ordered within the catalytic center. Thus, whereas the glutamate restricts the affinity for the coenzyme, the cysteine is the sensor of the coenzyme redox state. These conclusions were confirmed by coenzyme binding experiments. Our study further suggests that the binding of the coenzyme is additionally controlled by a long-range communication between the catalytic center and the coenzyme-binding domain and points toward an alpha-helix involved in the adenine moiety binding as a participant of this communication.	+
-		+
-	Conserved catalytic residues of the ALDH1L1 aldehyde dehydrogenase domain control binding and discharging of the coenzyme.,Tsybovsky Y, Krupenko SA J Biol Chem. 2011 Jul 1;286(26):23357-67. Epub 2011 May 3. PMID:21540484<ref>PMID:21540484</~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3rhl" style="background-color:#fffaf0;"><~~/~~div>~~	+

	==See Also==		==See Also==
	*[[Aldehyde dehydrogenase 3D structures\|Aldehyde dehydrogenase 3D structures]]		*[[Aldehyde dehydrogenase 3D structures\|Aldehyde dehydrogenase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Buffalo rat]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Tsybovsky, Y~~]]	+	[[Category: Rattus norvegicus]]
-	[[Category: ~~Fdh]]~~	+	[[Category: Tsybovsky Y]]
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 06:03, 15 June 2022

OCA — Wed, 15 Jun 2022 06:03:51 GMT

←Older revision		Revision as of 06:03, 15 June 2022
Line 1:		Line 1:

	==Crystal structure of the E673A/C707A double mutant of the C-Terminal domain of RAT 10'FORMYLTETRAHYDROFOLATE DEHYDROGENASE in complex with co-purified NADP==		==Crystal structure of the E673A/C707A double mutant of the C-Terminal domain of RAT 10'FORMYLTETRAHYDROFOLATE DEHYDROGENASE in complex with co-purified NADP==
-	<StructureSection load='3rhl' size='340' side='right' caption='[[3rhl]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='3rhl' size='340' side='right'caption='[[3rhl]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rhl]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RHL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3RHL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rhl]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RHL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RHL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rhj\|3rhj]], [[3rhm\|3rhm]], [[3rho\|3rho]], [[3rhp\|3rhp]], [[3rhq\|3rhq]], [[3rhr\|3rhr]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3rhj\|3rhj]], [[3rhm\|3rhm]], [[3rho\|3rho]], [[3rhp\|3rhp]], [[3rhq\|3rhq]], [[3rhr\|3rhr]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Aldh1l1, Fthfd ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Aldh1l1, Fthfd ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3rhl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rhl OCA], [~~http~~://pdbe.org/3rhl PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3rhl RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3rhl PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3rhl ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rhl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rhl OCA], [https://pdbe.org/3rhl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rhl RCSB], [https://www.ebi.ac.uk/pdbsum/3rhl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rhl ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 20:

	==See Also==		==See Also==
-	*[[Aldehyde dehydrogenase\|Aldehyde dehydrogenase]]	+	*[[Aldehyde dehydrogenase 3D structures\|Aldehyde dehydrogenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 26:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Buffalo rat]]		[[Category: Buffalo rat]]
		+	[[Category: Large Structures]]
	[[Category: Tsybovsky, Y]]		[[Category: Tsybovsky, Y]]
	[[Category: Fdh]]		[[Category: Fdh]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 11:20, 10 December 2016

OCA — Sat, 10 Dec 2016 11:20:14 GMT

←Older revision		Revision as of 11:20, 10 December 2016
Line 1:		Line 1:
		+
	==Crystal structure of the E673A/C707A double mutant of the C-Terminal domain of RAT 10'FORMYLTETRAHYDROFOLATE DEHYDROGENASE in complex with co-purified NADP==		==Crystal structure of the E673A/C707A double mutant of the C-Terminal domain of RAT 10'FORMYLTETRAHYDROFOLATE DEHYDROGENASE in complex with co-purified NADP==
	<StructureSection load='3rhl' size='340' side='right' caption='[[3rhl]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3rhl' size='340' side='right' caption='[[3rhl]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rhl]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Rattus_norvegicus Rattus norvegicus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RHL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RHL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rhl]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RHL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RHL FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rhj\|3rhj]], [[3rhm\|3rhm]], [[3rho\|3rho]], [[3rhp\|3rhp]], [[3rhq\|3rhq]], [[3rhr\|3rhr]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rhj\|3rhj]], [[3rhm\|3rhm]], [[3rho\|3rho]], [[3rhp\|3rhp]], [[3rhq\|3rhq]], [[3rhr\|3rhr]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Aldh1l1, Fthfd ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 ~~Rattus norvegicus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Aldh1l1, Fthfd ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rhl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rhl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rhl RCSB], [http://www.ebi.ac.uk/pdbsum/3rhl PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rhl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rhl OCA], [http://pdbe.org/3rhl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3rhl RCSB], [http://www.ebi.ac.uk/pdbsum/3rhl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3rhl ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3rhl" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 23:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Rattus norvegicus~~]]	+	[[Category: Buffalo rat]]
	[[Category: Tsybovsky, Y]]		[[Category: Tsybovsky, Y]]
	[[Category: Fdh]]		[[Category: Fdh]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 11:47, 9 December 2014

OCA — Tue, 09 Dec 2014 11:47:26 GMT

←Older revision		Revision as of 11:47, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3rhl.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of the E673A/C707A double mutant of the C-Terminal domain of RAT 10'FORMYLTETRAHYDROFOLATE DEHYDROGENASE in complex with co-purified NADP==
		+	<StructureSection load='3rhl' size='340' side='right' caption='[[3rhl]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3rhl]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RHL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RHL FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rhj\|3rhj]], [[3rhm\|3rhm]], [[3rho\|3rho]], [[3rhp\|3rhp]], [[3rhq\|3rhq]], [[3rhr\|3rhr]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Aldh1l1, Fthfd ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rhl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rhl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rhl RCSB], [http://www.ebi.ac.uk/pdbsum/3rhl PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The C-terminal domain (C(t)-FDH) of 10-formyltetrahydrofolate dehydrogenase (FDH, ALDH1L1) is an NADP(+)-dependent oxidoreductase and a structural and functional homolog of aldehyde dehydrogenases. Here we report the crystal structures of several C(t)-FDH mutants in which two essential catalytic residues adjacent to the nicotinamide ring of bound NADP(+), Cys-707 and Glu-673, were replaced separately or simultaneously. The replacement of the glutamate with an alanine causes irreversible binding of the coenzyme without any noticeable conformational changes in the vicinity of the nicotinamide ring. Additional replacement of cysteine 707 with an alanine (E673A/C707A double mutant) did not affect this irreversible binding indicating that the lack of the glutamate is solely responsible for the enhanced interaction between the enzyme and the coenzyme. The substitution of the cysteine with an alanine did not affect binding of NADP(+) but resulted in the enzyme lacking the ability to differentiate between the oxidized and reduced coenzyme: unlike the wild-type C(t)-FDH/NADPH complex, in the C707A mutant the position of NADPH is identical to the position of NADP(+) with the nicotinamide ring well ordered within the catalytic center. Thus, whereas the glutamate restricts the affinity for the coenzyme, the cysteine is the sensor of the coenzyme redox state. These conclusions were confirmed by coenzyme binding experiments. Our study further suggests that the binding of the coenzyme is additionally controlled by a long-range communication between the catalytic center and the coenzyme-binding domain and points toward an alpha-helix involved in the adenine moiety binding as a participant of this communication.

-	~~{{STRUCTURE_3rhl\| PDB=3rhl \| SCENE= }}~~	+	Conserved catalytic residues of the ALDH1L1 aldehyde dehydrogenase domain control binding and discharging of the coenzyme.,Tsybovsky Y, Krupenko SA J Biol Chem. 2011 Jul 1;286(26):23357-67. Epub 2011 May 3. PMID:21540484<ref>PMID:21540484</ref>

-	~~===Crystal structure of the E673A~~/~~C707A double mutant~~ of the ~~C-Terminal domain of RAT 10'FORMYLTETRAHYDROFOLATE DEHYDROGENASE in complex with co-purified NADP===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_21540484}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3rhl]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Rattus_norvegicus Rattus norvegicus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3RHL OCA].~~	+

	==See Also==		==See Also==
	*[[Aldehyde dehydrogenase\|Aldehyde dehydrogenase]]		*[[Aldehyde dehydrogenase\|Aldehyde dehydrogenase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:021540484<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Rattus norvegicus]]		[[Category: Rattus norvegicus]]
-	[[Category: Tsybovsky, Y.]]	+	[[Category: Tsybovsky, Y]]
	[[Category: Fdh]]		[[Category: Fdh]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 16:41, 20 October 2012

OCA — Sat, 20 Oct 2012 16:41:31 GMT

←Older revision		Revision as of 16:41, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3rhl]] is a 4 chain structure ~~of [[Aldehyde dehydrogenase]]~~ with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RHL OCA].	+	[[3rhl]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RHL OCA].

	==See Also==		==See Also==

OCA at 22:31, 26 July 2012

OCA — Thu, 26 Jul 2012 22:31:31 GMT

←Older revision		Revision as of 22:31, 26 July 2012
Line 1:		Line 1:
	[[Image:3rhl.png\|left\|200px]]		[[Image:3rhl.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3rhl", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3rhl\| PDB=3rhl \| SCENE= }}		{{STRUCTURE_3rhl\| PDB=3rhl \| SCENE= }}

	===Crystal structure of the E673A/C707A double mutant of the C-Terminal domain of RAT 10'FORMYLTETRAHYDROFOLATE DEHYDROGENASE in complex with co-purified NADP===		===Crystal structure of the E673A/C707A double mutant of the C-Terminal domain of RAT 10'FORMYLTETRAHYDROFOLATE DEHYDROGENASE in complex with co-purified NADP===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21540484}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 21540484 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_21540484}}		{{ABSTRACT_PUBMED_21540484}}

Line 22:		Line 11:

	==See Also==		==See Also==
-	*[[Aldehyde dehydrogenase]]	+	*[[Aldehyde dehydrogenase\|Aldehyde dehydrogenase]]

	==Reference==		==Reference==

OCA at 05:58, 31 August 2011

OCA — Wed, 31 Aug 2011 05:58:29 GMT

←Older revision		Revision as of 05:58, 31 August 2011
Line 1:		Line 1:
-	[[Image:3rhl.~~jpg~~\|left\|200px]]	+	[[Image:3rhl.png\|left\|200px]]

	<!--		<!--
Line 11:		Line 11:
	===Crystal structure of the E673A/C707A double mutant of the C-Terminal domain of RAT 10'FORMYLTETRAHYDROFOLATE DEHYDROGENASE in complex with co-purified NADP===		===Crystal structure of the E673A/C707A double mutant of the C-Terminal domain of RAT 10'FORMYLTETRAHYDROFOLATE DEHYDROGENASE in complex with co-purified NADP===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21540484}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21540484 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21540484}}

	==About this Structure==		==About this Structure==
-	[[3rhl]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RHL OCA].	+	[[3rhl]] is a 4 chain structure of [[Aldehyde dehydrogenase]] with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RHL OCA].
		+
		+	==See Also==
		+	*[[Aldehyde dehydrogenase]]
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021540484</ref><references group="xtra"/>
	[[Category: Rattus norvegicus]]		[[Category: Rattus norvegicus]]
	[[Category: Tsybovsky, Y.]]		[[Category: Tsybovsky, Y.]]
		+	[[Category: Fdh]]
		+	[[Category: Oxidoreductase]]

OCA at 07:31, 27 April 2011

OCA — Wed, 27 Apr 2011 07:31:26 GMT

←Older revision		Revision as of 07:31, 27 April 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3rhl.jpg\|left\|200px]]

-	The ~~entry 3rhl~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3rhl", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3rhl\| PDB=3rhl \| SCENE= }}

-	~~Authors: Tsybovsky, Y.~~	+	===Crystal structure of the E673A/C707A double mutant of the C-Terminal domain of RAT 10'FORMYLTETRAHYDROFOLATE DEHYDROGENASE in complex with co-purified NADP===

-	~~Description: Crystal~~ structure ~~of the E673A~~/~~C707A double mutant of the C~~-~~Terminal domain of RAT 10'FORMYLTETRAHYDROFOLATE DEHYDROGENASE in complex with co-purified NADP~~	+
		+	==About this Structure==
		+	[[3rhl]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RHL OCA].
		+	[[Category: Rattus norvegicus]]
		+	[[Category: Tsybovsky, Y.]]

OCA: Protected "3rhl" [edit=sysop:move=sysop]

OCA — Thu, 21 Apr 2011 10:23:17 GMT

Protected "3rhl" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:23, 21 April 2011

OCA: New page: '''Unreleased structure''' The entry 3rhl is ON HOLD Authors: Tsybovsky, Y. Description: Crystal structure of the E673A/C707A double mutant of the C-Terminal domain of RAT 10'FORMYLTET...

OCA — Thu, 21 Apr 2011 10:23:16 GMT

New page: '''Unreleased structure''' The entry 3rhl is ON HOLD Authors: Tsybovsky, Y. Description: Crystal structure of the E673A/C707A double mutant of the C-Terminal domain of RAT 10'FORMYLTET...

New page

'''Unreleased structure'''

The entry 3rhl is ON HOLD

Authors: Tsybovsky, Y.

Description: Crystal structure of the E673A/C707A double mutant of the C-Terminal domain of RAT 10'FORMYLTETRAHYDROFOLATE DEHYDROGENASE in complex with co-purified NADP