3riw - Revision history

OCA at 09:43, 1 March 2024

OCA — Fri, 01 Mar 2024 09:43:15 GMT

←Older revision		Revision as of 09:43, 1 March 2024
Line 3:		Line 3:
	<StructureSection load='3riw' size='340' side='right'caption='[[3riw]], [[Resolution\|resolution]] 2.37Å' scene=''>		<StructureSection load='3riw' size='340' side='right'caption='[[3riw]], [[Resolution\|resolution]] 2.37Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3riw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Leishmania_major_mhom/il/81/friedlin~~ Leishmania major ~~mhom/il/81/friedlin~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RIW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3riw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Leishmania_major_strain_Friedlin Leishmania major strain Friedlin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RIW FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~, ~~<scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.37Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[3riv\|3riv]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">APX~~, ~~LMJF_34_0070 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=347515 Leishmania major MHOM/IL/81/Friedlin])~~<~~/td></tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/L-ascorbate_peroxidase L-ascorbate peroxidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.11 1.11.1.11]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3riw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3riw OCA], [https://pdbe.org/3riw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3riw RCSB], [https://www.ebi.ac.uk/pdbsum/3riw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3riw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3riw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3riw OCA], [https://pdbe.org/3riw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3riw RCSB], [https://www.ebi.ac.uk/pdbsum/3riw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3riw ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q4Q3K2_LEIMA Q4Q3K2_LEIMA]
-	The parasitic protozoa, Leishmania major, produces a peroxidase (abbreviated LmP) that exhibits activities characteristic of both yeast cytochrome c peroxidase (CCP) and plant cytosolic ascorbate peroxidase (APX). One common feature is a key Trp residue, Trp208 in LmP and Trp191 in CCP, that is situated adjacent to the proximal His heme ligand in CCP, APX and LmP. In CCP Trp191 forms a stable cationic radical after reaction with H2O2 to form Compound I; in APX the radical is located on the porphyrin ring. In order to clarify the role of Trp208 in LmP and to further probe peroxidase structure-function relationships we have determined the crystal structure of LmP and have studied the role of Trp208 using electron paramagnetic resonance spectroscopy (EPR), mutagenesis, and enzyme kinetics. Both CCP and LmP have an extended section of beta structure near Trp191 and 208, respectively, which is absent in APX. This region provides stability to the Trp191 radical in CCP. EPR of LmP Compound I exhibits an intense and stable signal similar to CCP Compound I. In the LmP Trp208Phe mutant this signal disappears indicating that Trp208 forms a stable cationic radical. In LmP conversion of the Cys197 to Thr significantly weakens the Compound I EPR signal and dramatically lowers enzyme activity. These results further support the view that modulation of the local electrostatic environment controls the stability of the Trp radical in peroxidases. Our results also suggest that the biological role of LmP is to function as a cytochrome c peroxdase.	+
-		+
-	~~The crystal structure of Leishmania major peroxidase and characterization of the compound I tryptophan radical.,Jasion VS, Polanco JA, Meharenna YT, Li H, Poulos TL J Biol Chem. 2011 May 13. PMID~~:~~21566139<ref>PMID:21566139<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3riw" style="background-color:#fffaf0;"><~~/~~div>~~	+

	==See Also==		==See Also==
	*[[Ascorbate peroxidase 3D structures\|Ascorbate peroxidase 3D structures]]		*[[Ascorbate peroxidase 3D structures\|Ascorbate peroxidase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: L-ascorbate peroxidase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Leishmania major ~~mhom/il/81/friedlin~~]]	+	[[Category: Leishmania major strain Friedlin]]
-	[[Category: Jasion~~, V S~~]]	+	[[Category: Jasion VS]]
-	[[Category: Li, H]]	+	[[Category: Li H]]
-	[[Category: Poulos~~, T L]]~~	+	[[Category: Poulos TL]]
-	~~[[Category: Alpha helical bundle]]~~	+
-	~~[[Category: Heme peroxidase]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 10:12, 22 June 2022

OCA — Wed, 22 Jun 2022 10:12:23 GMT

←Older revision		Revision as of 10:12, 22 June 2022
Line 1:		Line 1:

	==The Crystal Structure of Leishmania major Peroxidase mutant C197T==		==The Crystal Structure of Leishmania major Peroxidase mutant C197T==
-	<StructureSection load='3riw' size='340' side='right' caption='[[3riw]], [[Resolution\|resolution]] 2.37Å' scene=''>	+	<StructureSection load='3riw' size='340' side='right'caption='[[3riw]], [[Resolution\|resolution]] 2.37Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3riw]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Leishmania_major_mhom/il/81/friedlin Leishmania major mhom/il/81/friedlin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3RIW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3riw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Leishmania_major_mhom/il/81/friedlin Leishmania major mhom/il/81/friedlin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RIW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3riv\|3riv]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3riv\|3riv]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">APX, LMJF_34_0070 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=347515 Leishmania major MHOM/IL/81/Friedlin])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">APX, LMJF_34_0070 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=347515 Leishmania major MHOM/IL/81/Friedlin])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/L-ascorbate_peroxidase L-ascorbate peroxidase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.11 1.11.1.11] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/L-ascorbate_peroxidase L-ascorbate peroxidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.11 1.11.1.11] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3riw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3riw OCA], [~~http~~://pdbe.org/3riw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3riw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3riw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3riw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3riw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3riw OCA], [https://pdbe.org/3riw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3riw RCSB], [https://www.ebi.ac.uk/pdbsum/3riw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3riw ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 3riw" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3riw" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ascorbate peroxidase 3D structures\|Ascorbate peroxidase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: L-ascorbate peroxidase]]		[[Category: L-ascorbate peroxidase]]
		+	[[Category: Large Structures]]
	[[Category: Leishmania major mhom/il/81/friedlin]]		[[Category: Leishmania major mhom/il/81/friedlin]]
	[[Category: Jasion, V S]]		[[Category: Jasion, V S]]

OCA at 09:26, 8 November 2017

OCA — Wed, 08 Nov 2017 09:26:42 GMT

←Older revision		Revision as of 09:26, 8 November 2017
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 3riw" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3riw" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Ascorbate peroxidase\|Ascorbate peroxidase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 05:37, 5 August 2016

OCA — Fri, 05 Aug 2016 05:37:08 GMT

←Older revision		Revision as of 05:37, 5 August 2016
Line 1:		Line 1:
		+
	==The Crystal Structure of Leishmania major Peroxidase mutant C197T==		==The Crystal Structure of Leishmania major Peroxidase mutant C197T==
	<StructureSection load='3riw' size='340' side='right' caption='[[3riw]], [[Resolution\|resolution]] 2.37Å' scene=''>		<StructureSection load='3riw' size='340' side='right' caption='[[3riw]], [[Resolution\|resolution]] 2.37Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3riw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Leishmania_major~~ Leishmania major]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RIW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3riw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Leishmania_major_mhom/il/81/friedlin Leishmania major mhom/il/81/friedlin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RIW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3riv\|3riv]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3riv\|3riv]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">APX, LMJF_34_0070 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~5664~~ Leishmania major])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">APX, LMJF_34_0070 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=347515 Leishmania major MHOM/IL/81/Friedlin])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-ascorbate_peroxidase L-ascorbate peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.11 1.11.1.11] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-ascorbate_peroxidase L-ascorbate peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.11 1.11.1.11] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3riw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3riw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3riw RCSB], [http://www.ebi.ac.uk/pdbsum/3riw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3riw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3riw OCA], [http://pdbe.org/3riw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3riw RCSB], [http://www.ebi.ac.uk/pdbsum/3riw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3riw ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3riw" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: L-ascorbate peroxidase]]		[[Category: L-ascorbate peroxidase]]
-	[[Category: Leishmania major]]	+	[[Category: Leishmania major mhom/il/81/friedlin]]
	[[Category: Jasion, V S]]		[[Category: Jasion, V S]]
	[[Category: Li, H]]		[[Category: Li, H]]

OCA at 08:26, 18 December 2014

OCA — Thu, 18 Dec 2014 08:26:37 GMT

←Older revision		Revision as of 08:26, 18 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3riw\| PDB=3riw \| SCENE= }}~~	+	==The Crystal Structure of Leishmania major Peroxidase mutant C197T==
-	===The Crystal Structure of Leishmania major Peroxidase mutant C197T===	+	<StructureSection load='3riw' size='340' side='right' caption='[[3riw]], [[Resolution\|resolution]] 2.37Å' scene=''>
-	~~{{ABSTRACT_PUBMED_21566139}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3riw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Leishmania_major Leishmania major]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RIW FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3riv\|3riv]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">APX, LMJF_34_0070 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5664 Leishmania major])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-ascorbate_peroxidase L-ascorbate peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.11 1.11.1.11] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3riw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3riw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3riw RCSB], [http://www.ebi.ac.uk/pdbsum/3riw PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The parasitic protozoa, Leishmania major, produces a peroxidase (abbreviated LmP) that exhibits activities characteristic of both yeast cytochrome c peroxidase (CCP) and plant cytosolic ascorbate peroxidase (APX). One common feature is a key Trp residue, Trp208 in LmP and Trp191 in CCP, that is situated adjacent to the proximal His heme ligand in CCP, APX and LmP. In CCP Trp191 forms a stable cationic radical after reaction with H2O2 to form Compound I; in APX the radical is located on the porphyrin ring. In order to clarify the role of Trp208 in LmP and to further probe peroxidase structure-function relationships we have determined the crystal structure of LmP and have studied the role of Trp208 using electron paramagnetic resonance spectroscopy (EPR), mutagenesis, and enzyme kinetics. Both CCP and LmP have an extended section of beta structure near Trp191 and 208, respectively, which is absent in APX. This region provides stability to the Trp191 radical in CCP. EPR of LmP Compound I exhibits an intense and stable signal similar to CCP Compound I. In the LmP Trp208Phe mutant this signal disappears indicating that Trp208 forms a stable cationic radical. In LmP conversion of the Cys197 to Thr significantly weakens the Compound I EPR signal and dramatically lowers enzyme activity. These results further support the view that modulation of the local electrostatic environment controls the stability of the Trp radical in peroxidases. Our results also suggest that the biological role of LmP is to function as a cytochrome c peroxdase.

-	~~==About this Structure==~~	+	The crystal structure of Leishmania major peroxidase and characterization of the compound I tryptophan radical.,Jasion VS, Polanco JA, Meharenna YT, Li H, Poulos TL J Biol Chem. 2011 May 13. PMID:21566139<ref>PMID:21566139</ref>
-	~~[[3riw]] is a 2 chain~~ structure ~~with sequence from [http://en.wikipedia.org/wiki/Leishmania_major~~ Leishmania major]. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=3RIW OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[Ascorbate peroxidase\|Ascorbate peroxidase]]		*[[Ascorbate peroxidase\|Ascorbate peroxidase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:021566139<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: L-ascorbate peroxidase]]		[[Category: L-ascorbate peroxidase]]
	[[Category: Leishmania major]]		[[Category: Leishmania major]]
-	[[Category: Jasion, V S.]]	+	[[Category: Jasion, V S]]
-	[[Category: Li, H.]]	+	[[Category: Li, H]]
-	[[Category: Poulos, T L.]]	+	[[Category: Poulos, T L]]
	[[Category: Alpha helical bundle]]		[[Category: Alpha helical bundle]]
	[[Category: Heme peroxidase]]		[[Category: Heme peroxidase]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 10:20, 11 March 2013

OCA — Mon, 11 Mar 2013 10:20:33 GMT

←Older revision		Revision as of 10:20, 11 March 2013
Line 1:		Line 1:
-	[[Image:3riw.png\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3riw", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3riw\| PDB=3riw \| SCENE= }}		{{STRUCTURE_3riw\| PDB=3riw \| SCENE= }}
-
	===The Crystal Structure of Leishmania major Peroxidase mutant C197T===		===The Crystal Structure of Leishmania major Peroxidase mutant C197T===
-
-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21566139}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 21566139 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_21566139}}		{{ABSTRACT_PUBMED_21566139}}

	==About this Structure==		==About this Structure==
	[[3riw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Leishmania_major Leishmania major]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIW OCA].		[[3riw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Leishmania_major Leishmania major]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIW OCA].
		+
		+	==See Also==
		+	*[[Ascorbate peroxidase\|Ascorbate peroxidase]]

	==Reference==		==Reference==

OCA at 05:06, 27 July 2011

OCA — Wed, 27 Jul 2011 05:06:14 GMT

←Older revision		Revision as of 05:06, 27 July 2011
Line 28:		Line 28:
	[[Category: Li, H.]]		[[Category: Li, H.]]
	[[Category: Poulos, T L.]]		[[Category: Poulos, T L.]]
		+	[[Category: Alpha helical bundle]]
		+	[[Category: Heme peroxidase]]
		+	[[Category: Oxidoreductase]]

OCA at 05:28, 25 May 2011

OCA — Wed, 25 May 2011 05:28:36 GMT

←Older revision		Revision as of 05:28, 25 May 2011
Line 1:		Line 1:
-	[[Image:3riw.~~jpg~~\|left\|200px]]	+	[[Image:3riw.png\|left\|200px]]

	<!--		<!--
Line 11:		Line 11:
	===The Crystal Structure of Leishmania major Peroxidase mutant C197T===		===The Crystal Structure of Leishmania major Peroxidase mutant C197T===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21566139}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21566139 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21566139}}

	==About this Structure==		==About this Structure==
	[[3riw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Leishmania_major Leishmania major]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIW OCA].		[[3riw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Leishmania_major Leishmania major]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIW OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021566139</ref><references group="xtra"/>
	[[Category: L-ascorbate peroxidase]]		[[Category: L-ascorbate peroxidase]]
	[[Category: Leishmania major]]		[[Category: Leishmania major]]

OCA at 04:55, 12 May 2011

OCA — Thu, 12 May 2011 04:55:25 GMT

←Older revision		Revision as of 04:55, 12 May 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3riw.jpg\|left\|200px]]

-	The ~~entry 3riw~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3riw", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3riw\| PDB=3riw \| SCENE= }}

-	~~Authors: Jasion, V.S., Li, H., Poulos, T.L.~~	+	===The Crystal Structure of Leishmania major Peroxidase mutant C197T===

-	~~Description: The Crystal~~ Structure of Leishmania major ~~Peroxidase mutant C197T~~	+
		+	==About this Structure==
		+	[[3riw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Leishmania_major Leishmania major]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RIW OCA].
		+	[[Category: L-ascorbate peroxidase]]
		+	[[Category: Leishmania major]]
		+	[[Category: Jasion, V S.]]
		+	[[Category: Li, H.]]
		+	[[Category: Poulos, T L.]]

OCA at 06:56, 27 April 2011

OCA — Wed, 27 Apr 2011 06:56:28 GMT

←Older revision		Revision as of 06:56, 27 April 2011
Line 5:		Line 5:
	Authors: Jasion, V.S., Li, H., Poulos, T.L.		Authors: Jasion, V.S., Li, H., Poulos, T.L.

-	Description: The Crystal Structure of Leishmania major Peroxidase; mutant C197T.	+	Description: The Crystal Structure of Leishmania major Peroxidase mutant C197T