3ro5 - Revision history

OCA at 09:44, 1 March 2024

OCA — Fri, 01 Mar 2024 09:44:11 GMT

←Older revision		Revision as of 09:44, 1 March 2024
Line 3:		Line 3:
	<StructureSection load='3ro5' size='340' side='right'caption='[[3ro5]], [[Resolution\|resolution]] 2.66Å' scene=''>		<StructureSection load='3ro5' size='340' side='right'caption='[[3ro5]], [[Resolution\|resolution]] 2.66Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ro5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~I33a0 I33a0~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RO5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RO5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ro5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_A_virus_(A/Wilson-Smith/1933(H1N1)) Influenza A virus (A/Wilson-Smith/1933(H1N1))]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RO5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RO5 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LGH:[4-(2-CHLORO-4-NITROPHENYL)PIPERAZIN-1-YL][3-(2-METHOXYPHENYL)-5-METHYL-1,2-OXAZOL-4-YL]METHANONE'>LGH</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.66Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NP ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381518 I33A0])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LGH:[4-(2-CHLORO-4-NITROPHENYL)PIPERAZIN-1-YL][3-(2-METHOXYPHENYL)-5-METHYL-1,2-OXAZOL-4-YL]METHANONE'>LGH</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ro5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ro5 OCA], [https://pdbe.org/3ro5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ro5 RCSB], [https://www.ebi.ac.uk/pdbsum/3ro5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ro5 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ro5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ro5 OCA], [https://pdbe.org/3ro5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ro5 RCSB], [https://www.ebi.ac.uk/pdbsum/3ro5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ro5 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/Q1K9H2_I33A0 Q1K9H2_I33A0]] Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus (By similarity).[SAAS:SAAS002141_004_603280]	+	[https://www.uniprot.org/uniprot/Q1K9H2_I33A0 Q1K9H2_I33A0] Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus (By similarity).[SAAS:SAAS002141_004_603280]
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Influenza nucleoprotein (NP) plays multiple roles in the virus life cycle, including an essential function in viral replication as an integral component of the ribonucleoprotein complex, associating with viral RNA and polymerase within the viral core. The multifunctional nature of NP makes it an attractive target for antiviral intervention, and inhibitors targeting this protein have recently been reported. In a parallel effort, we discovered a structurally similar series of influenza replication inhibitors and show that they interfere with NP-dependent processes via formation of higher-order NP oligomers. Support for this unique mechanism is provided by site-directed mutagenesis studies, biophysical characterization of the oligomeric ligand:NP complex, and an X-ray cocrystal structure of an NP dimer of trimers (or hexamer) comprising three NP_A:NP_B dimeric subunits. Each NP_A:NP_B dimeric subunit contains two ligands that bridge two composite, protein-spanning binding sites in an antiparallel orientation to form a stable quaternary complex. Optimization of the initial screening hit produced an analog that protects mice from influenza-induced weight loss and mortality by reducing viral titers to undetectable levels throughout the course of treatment.	+
-		+
-	Inhibition of influenza virus replication via small molecules that induce the formation of higher-order nucleoprotein oligomers.,Gerritz SW, Cianci C, Kim S, Pearce BC, Deminie C, Discotto L, McAuliffe B, Minassian BF, Shi S, Zhu S, Zhai W, Pendri A, Li G, Poss MA, Edavettal S, McDonnell PA, Lewis HA, Maskos K, Mortl M, Kiefersauer R, Steinbacher S, Baldwin ET, Metzler W, Bryson J, Healy MD, Philip T, Zoeckler M, Schartman R, Sinz M, Leyva-Grado VH, Hoffmann HH, Langley DR, Meanwell NA, Krystal M Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):15366-71. Epub 2011 Sep 6. PMID:21896751<ref>PMID:21896751</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3ro5" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Nucleoprotein 3D structures\|Nucleoprotein 3D structures]]		*[[Nucleoprotein 3D structures\|Nucleoprotein 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: I33a0]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Baldwin~~, E T~~]]	+	[[Category: Baldwin ET]]
-	[[Category: Edavettal, S]]	+	[[Category: Edavettal S]]
-	[[Category: Kiefersauer, R]]	+	[[Category: Kiefersauer R]]
-	[[Category: Langley~~, D R~~]]	+	[[Category: Langley DR]]
-	[[Category: Lewis~~, H A~~]]	+	[[Category: Lewis HA]]
-	[[Category: Maskos, K]]	+	[[Category: Maskos K]]
-	[[Category: McDonnell~~, P A~~]]	+	[[Category: McDonnell PA]]
-	[[Category: Mortl, M]]	+	[[Category: Mortl M]]
-	[[Category: Pearce~~, B C~~]]	+	[[Category: Pearce BC]]
-	[[Category: Steinbacher, S]]	+	[[Category: Steinbacher S]]
-	~~[[Category: Influenza nucleoprotein]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 10:19, 22 June 2022

OCA — Wed, 22 Jun 2022 10:19:13 GMT

←Older revision		Revision as of 10:19, 22 June 2022
Line 1:		Line 1:

	==Crystal structure of influenza A virus nucleoprotein with ligand==		==Crystal structure of influenza A virus nucleoprotein with ligand==
-	<StructureSection load='3ro5' size='340' side='right' caption='[[3ro5]], [[Resolution\|resolution]] 2.66Å' scene=''>	+	<StructureSection load='3ro5' size='340' side='right'caption='[[3ro5]], [[Resolution\|resolution]] 2.66Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ro5]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/I33a0 I33a0]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RO5 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3RO5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ro5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/I33a0 I33a0]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RO5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RO5 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LGH:[4-(2-CHLORO-4-NITROPHENYL)PIPERAZIN-1-YL][3-(2-METHOXYPHENYL)-5-METHYL-1,2-OXAZOL-4-YL]METHANONE'>LGH</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LGH:[4-(2-CHLORO-4-NITROPHENYL)PIPERAZIN-1-YL][3-(2-METHOXYPHENYL)-5-METHYL-1,2-OXAZOL-4-YL]METHANONE'>LGH</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NP ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381518 I33A0])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NP ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381518 I33A0])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ro5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ro5 OCA], [~~http~~://pdbe.org/3ro5 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3ro5 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3ro5 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3ro5 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ro5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ro5 OCA], [https://pdbe.org/3ro5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ro5 RCSB], [https://www.ebi.ac.uk/pdbsum/3ro5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ro5 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/Q1K9H2_I33A0 Q1K9H2_I33A0]] Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus (By similarity).[SAAS:SAAS002141_004_603280]	+	[[https://www.uniprot.org/uniprot/Q1K9H2_I33A0 Q1K9H2_I33A0]] Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus (By similarity).[SAAS:SAAS002141_004_603280]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:

	==See Also==		==See Also==
-	*[[Nucleoprotein\|Nucleoprotein]]	+	*[[Nucleoprotein 3D structures\|Nucleoprotein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 27:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: I33a0]]		[[Category: I33a0]]
		+	[[Category: Large Structures]]
	[[Category: Baldwin, E T]]		[[Category: Baldwin, E T]]
	[[Category: Edavettal, S]]		[[Category: Edavettal, S]]

OCA at 11:30, 10 December 2016

OCA — Sat, 10 Dec 2016 11:30:54 GMT

←Older revision		Revision as of 11:30, 10 December 2016
Line 1:		Line 1:
		+
	==Crystal structure of influenza A virus nucleoprotein with ligand==		==Crystal structure of influenza A virus nucleoprotein with ligand==
	<StructureSection load='3ro5' size='340' side='right' caption='[[3ro5]], [[Resolution\|resolution]] 2.66Å' scene=''>		<StructureSection load='3ro5' size='340' side='right' caption='[[3ro5]], [[Resolution\|resolution]] 2.66Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3ro5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Influenza_a_virus Influenza a virus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RO5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RO5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3ro5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/I33a0 I33a0]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RO5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RO5 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LGH:[4-(2-CHLORO-4-NITROPHENYL)PIPERAZIN-1-YL][3-(2-METHOXYPHENYL)-5-METHYL-1,2-OXAZOL-4-YL]METHANONE'>LGH</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LGH:[4-(2-CHLORO-4-NITROPHENYL)PIPERAZIN-1-YL][3-(2-METHOXYPHENYL)-5-METHYL-1,2-OXAZOL-4-YL]METHANONE'>LGH</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~11320 Influenza A virus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381518 I33A0])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ro5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ro5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ro5 RCSB], [http://www.ebi.ac.uk/pdbsum/3ro5 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ro5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ro5 OCA], [http://pdbe.org/3ro5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ro5 RCSB], [http://www.ebi.ac.uk/pdbsum/3ro5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ro5 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q1K9H2_I33A0 Q1K9H2_I33A0]] Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus (By similarity).[SAAS:SAAS002141_004_603280]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 15:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3ro5" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 22:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Influenza a virus~~]]	+	[[Category: I33a0]]
	[[Category: Baldwin, E T]]		[[Category: Baldwin, E T]]
	[[Category: Edavettal, S]]		[[Category: Edavettal, S]]

OCA at 11:25, 9 December 2014

OCA — Tue, 09 Dec 2014 11:25:41 GMT

←Older revision		Revision as of 11:25, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3ro5.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of influenza A virus nucleoprotein with ligand==
		+	<StructureSection load='3ro5' size='340' side='right' caption='[[3ro5]], [[Resolution\|resolution]] 2.66Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3ro5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RO5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RO5 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LGH:[4-(2-CHLORO-4-NITROPHENYL)PIPERAZIN-1-YL][3-(2-METHOXYPHENYL)-5-METHYL-1,2-OXAZOL-4-YL]METHANONE'>LGH</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11320 Influenza A virus])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ro5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ro5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ro5 RCSB], [http://www.ebi.ac.uk/pdbsum/3ro5 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Influenza nucleoprotein (NP) plays multiple roles in the virus life cycle, including an essential function in viral replication as an integral component of the ribonucleoprotein complex, associating with viral RNA and polymerase within the viral core. The multifunctional nature of NP makes it an attractive target for antiviral intervention, and inhibitors targeting this protein have recently been reported. In a parallel effort, we discovered a structurally similar series of influenza replication inhibitors and show that they interfere with NP-dependent processes via formation of higher-order NP oligomers. Support for this unique mechanism is provided by site-directed mutagenesis studies, biophysical characterization of the oligomeric ligand:NP complex, and an X-ray cocrystal structure of an NP dimer of trimers (or hexamer) comprising three NP_A:NP_B dimeric subunits. Each NP_A:NP_B dimeric subunit contains two ligands that bridge two composite, protein-spanning binding sites in an antiparallel orientation to form a stable quaternary complex. Optimization of the initial screening hit produced an analog that protects mice from influenza-induced weight loss and mortality by reducing viral titers to undetectable levels throughout the course of treatment.

-	~~{{STRUCTURE_3ro5\| PDB=3ro5 \| SCENE= }}~~	+	Inhibition of influenza virus replication via small molecules that induce the formation of higher-order nucleoprotein oligomers.,Gerritz SW, Cianci C, Kim S, Pearce BC, Deminie C, Discotto L, McAuliffe B, Minassian BF, Shi S, Zhu S, Zhai W, Pendri A, Li G, Poss MA, Edavettal S, McDonnell PA, Lewis HA, Maskos K, Mortl M, Kiefersauer R, Steinbacher S, Baldwin ET, Metzler W, Bryson J, Healy MD, Philip T, Zoeckler M, Schartman R, Sinz M, Leyva-Grado VH, Hoffmann HH, Langley DR, Meanwell NA, Krystal M Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):15366-71. Epub 2011 Sep 6. PMID:21896751<ref>PMID:21896751</ref>

-	~~===Crystal structure of influenza A virus nucleoprotein with ligand===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_21896751}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3ro5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki~~/~~Influenza_a_virus Influenza~~ a ~~virus]~~. ~~Full crystallographic information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=3RO5 OCA].~~	+

	==See Also==		==See Also==
	*[[Nucleoprotein\|Nucleoprotein]]		*[[Nucleoprotein\|Nucleoprotein]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:021896751<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Influenza a virus]]		[[Category: Influenza a virus]]
-	[[Category: Baldwin, E T.]]	+	[[Category: Baldwin, E T]]
-	[[Category: Edavettal, S.]]	+	[[Category: Edavettal, S]]
-	[[Category: Kiefersauer, R.]]	+	[[Category: Kiefersauer, R]]
-	[[Category: Langley, D R.]]	+	[[Category: Langley, D R]]
-	[[Category: Lewis, H A.]]	+	[[Category: Lewis, H A]]
-	[[Category: Maskos, K.]]	+	[[Category: Maskos, K]]
-	[[Category: McDonnell, P A.]]	+	[[Category: McDonnell, P A]]
-	[[Category: Mortl, M.]]	+	[[Category: Mortl, M]]
-	[[Category: Pearce, B C.]]	+	[[Category: Pearce, B C]]
-	[[Category: Steinbacher, S.]]	+	[[Category: Steinbacher, S]]
	[[Category: Influenza nucleoprotein]]		[[Category: Influenza nucleoprotein]]
	[[Category: Viral protein]]		[[Category: Viral protein]]

OCA at 20:25, 20 October 2012

OCA — Sat, 20 Oct 2012 20:25:15 GMT

←Older revision		Revision as of 20:25, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3ro5]] is a 2 chain structure ~~of [[Nucleoprotein]]~~ with sequence from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RO5 OCA].	+	[[3ro5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RO5 OCA].

	==See Also==		==See Also==

OCA at 10:25, 27 July 2012

OCA — Fri, 27 Jul 2012 10:25:19 GMT

←Older revision		Revision as of 10:25, 27 July 2012
Line 1:		Line 1:
	[[Image:3ro5.png\|left\|200px]]		[[Image:3ro5.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3ro5", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3ro5\| PDB=3ro5 \| SCENE= }}		{{STRUCTURE_3ro5\| PDB=3ro5 \| SCENE= }}

	===Crystal structure of influenza A virus nucleoprotein with ligand===		===Crystal structure of influenza A virus nucleoprotein with ligand===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21896751}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 21896751 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_21896751}}		{{ABSTRACT_PUBMED_21896751}}

	==About this Structure==		==About this Structure==
-	[[3ro5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RO5 OCA].	+	[[3ro5]] is a 2 chain structure of [[Nucleoprotein]] with sequence from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RO5 OCA].
		+
		+	==See Also==
		+	*[[Nucleoprotein\|Nucleoprotein]]

	==Reference==		==Reference==

OCA at 05:12, 21 September 2011

OCA — Wed, 21 Sep 2011 05:12:08 GMT

←Older revision		Revision as of 05:12, 21 September 2011
Line 1:		Line 1:
-	[[Image:3ro5.~~jpg~~\|left\|200px]]	+	[[Image:3ro5.png\|left\|200px]]

	<!--		<!--
Line 11:		Line 11:
	===Crystal structure of influenza A virus nucleoprotein with ligand===		===Crystal structure of influenza A virus nucleoprotein with ligand===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21896751}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21896751 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21896751}}

	==About this Structure==		==About this Structure==
	[[3ro5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RO5 OCA].		[[3ro5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RO5 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021896751</ref><references group="xtra"/>
	[[Category: Influenza a virus]]		[[Category: Influenza a virus]]
	[[Category: Baldwin, E T.]]		[[Category: Baldwin, E T.]]

OCA at 06:33, 14 September 2011

OCA — Wed, 14 Sep 2011 06:33:56 GMT

←Older revision		Revision as of 06:33, 14 September 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3ro5.jpg\|left\|200px]]

-	The ~~entry 3ro5~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3ro5", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3ro5\| PDB=3ro5 \| SCENE= }}

-	~~Authors: Pearce, B.C., Edavettal, S., McDonnell, P.~~A~~., Lewis, H.A., Steinbacher, S., Baldwin, E.T., Langley, D.R., Maskos, K., Mortl, M., Kiefersauer, R.~~	+	===Crystal structure of influenza A virus nucleoprotein with ligand===

-	~~Description: Crystal~~ structure ~~of influenza A~~ virus nucleoprotein ~~with ligand~~	+
		+	==About this Structure==
		+	[[3ro5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RO5 OCA].
		+	[[Category: Influenza a virus]]
		+	[[Category: Baldwin, E T.]]
		+	[[Category: Edavettal, S.]]
		+	[[Category: Kiefersauer, R.]]
		+	[[Category: Langley, D R.]]
		+	[[Category: Lewis, H A.]]
		+	[[Category: Maskos, K.]]
		+	[[Category: McDonnell, P A.]]
		+	[[Category: Mortl, M.]]
		+	[[Category: Pearce, B C.]]
		+	[[Category: Steinbacher, S.]]
		+	[[Category: Influenza nucleoprotein]]
		+	[[Category: Viral protein]]

OCA at 05:51, 15 June 2011

OCA — Wed, 15 Jun 2011 05:51:37 GMT

←Older revision		Revision as of 05:51, 15 June 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3ro5 is ON HOLD	+	The entry 3ro5 is ON HOLD until Paper Publication

	Authors: Pearce, B.C., Edavettal, S., McDonnell, P.A., Lewis, H.A., Steinbacher, S., Baldwin, E.T., Langley, D.R., Maskos, K., Mortl, M., Kiefersauer, R.		Authors: Pearce, B.C., Edavettal, S., McDonnell, P.A., Lewis, H.A., Steinbacher, S., Baldwin, E.T., Langley, D.R., Maskos, K., Mortl, M., Kiefersauer, R.

	Description: Crystal structure of influenza A virus nucleoprotein with ligand		Description: Crystal structure of influenza A virus nucleoprotein with ligand

OCA: Protected "3ro5" [edit=sysop:move=sysop]

OCA — Wed, 04 May 2011 06:34:10 GMT

Protected "3ro5" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:34, 4 May 2011