3rod - Revision history

OCA at 12:31, 14 March 2024

OCA — Thu, 14 Mar 2024 12:31:30 GMT

←Older revision		Revision as of 12:31, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3rod' size='340' side='right'caption='[[3rod]], [[Resolution\|resolution]] 2.72Å' scene=''>		<StructureSection load='3rod' size='340' side='right'caption='[[3rod]], [[Resolution\|resolution]] 2.72Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rod]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ROD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ROD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rod]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ROD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ROD FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NCA:NICOTINAMIDE'>NCA</scene>~~, ~~<scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.72Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>NNMT ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~9606 HUMAN])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NCA:NICOTINAMIDE'>NCA</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[https~~:~~//en.wikipedia.org/wiki/Nicotinamide_N~~-~~methyltransferase Nicotinamide N~~-~~methyltransferase], with EC number [https://www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=2.1.1.1 2.1.1.1]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rod OCA], [https://pdbe.org/3rod PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rod RCSB], [https://www.ebi.ac.uk/pdbsum/3rod PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rod ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rod OCA], [https://pdbe.org/3rod PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rod RCSB], [https://www.ebi.ac.uk/pdbsum/3rod PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rod ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/NNMT_HUMAN NNMT_HUMAN]] Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.	+	[https://www.uniprot.org/uniprot/NNMT_HUMAN NNMT_HUMAN] Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Nicotinamide N-methyltransferase (NNMT) catalyzes the N-methylation of nicotinamide, pyridines, and other analogues using S-adenosyl-l-methionine as donor. NNMT plays a significant role in the regulation of metabolic pathways and is expressed at markedly high levels in several kinds of cancers, presenting it as a potential molecular target for cancer therapy. We have determined the crystal structure of human NNMT as a ternary complex bound to both the demethylated donor S-adenosyl-l-homocysteine and the acceptor substrate nicotinamide, to 2.7 A resolution. These studies reveal the structural basis for nicotinamide binding and highlight several residues in the active site which may play roles in nicotinamide recognition and NNMT catalysis. The functional importance of these residues was probed by mutagenesis. Of three residues near the nicotinamide's amide group, substitution of S201 and S213 had no effect on enzyme activity while replacement of D197 dramatically decreased activity. Substitutions of Y20, whose side chain hydroxyl interacts with both the nicotinamide aromatic ring and AdoHcy carboxylate, also compromised activity. Enzyme kinetics analysis revealed k(cat)/K(m) decreases of 2-3 orders of magnitude for the D197A and Y20A mutants, confirming the functional importance of these active site residues. The mutants exhibited substantially increased K(m) for both NCA and AdoMet and modestly decreased k(cat). MD simulations revealed long-range conformational effects which provide an explanation for the large increase in K(m)(AdoMet) for the D197A mutant, which interacts directly only with nicotinamide in the ternary complex crystal structure.	+
-		+
-	Structural Basis of Substrate Recognition in Human Nicotinamide N-Methyltransferase.,Peng Y, Sartini D, Pozzi V, Wilk D, Emanuelli M, Yee VC Biochemistry. 2011 Aug 17. PMID:21823666<ref>PMID:21823666</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3rod" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Nicotinamide N-methyltransferase]]~~	+	[[Category: Peng Y]]
-	[[Category: Peng, Y]]	+	[[Category: Yee VC]]
-	[[Category: Yee~~, V C]]~~	+
-	~~[[Category: Methyltransferase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 10:19, 22 June 2022

OCA — Wed, 22 Jun 2022 10:19:31 GMT

←Older revision		Revision as of 10:19, 22 June 2022
Line 1:		Line 1:

	==Methyltransferase==		==Methyltransferase==
-	<StructureSection load='3rod' size='340' side='right' caption='[[3rod]], [[Resolution\|resolution]] 2.72Å' scene=''>	+	<StructureSection load='3rod' size='340' side='right'caption='[[3rod]], [[Resolution\|resolution]] 2.72Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rod]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ROD OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3ROD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rod]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ROD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ROD FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NCA:NICOTINAMIDE'>NCA</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NCA:NICOTINAMIDE'>NCA</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NNMT ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NNMT ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Nicotinamide_N-methyltransferase Nicotinamide N-methyltransferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.1 2.1.1.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Nicotinamide_N-methyltransferase Nicotinamide N-methyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.1 2.1.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3rod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rod OCA], [~~http~~://pdbe.org/3rod PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3rod RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3rod PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3rod ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rod OCA], [https://pdbe.org/3rod PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rod RCSB], [https://www.ebi.ac.uk/pdbsum/3rod PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rod ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/NNMT_HUMAN NNMT_HUMAN]] Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.	+	[[https://www.uniprot.org/uniprot/NNMT_HUMAN NNMT_HUMAN]] Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 25:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Nicotinamide N-methyltransferase]]		[[Category: Nicotinamide N-methyltransferase]]
	[[Category: Peng, Y]]		[[Category: Peng, Y]]

OCA at 22:19, 5 August 2016

OCA — Fri, 05 Aug 2016 22:19:43 GMT

←Older revision		Revision as of 22:19, 5 August 2016
Line 1:		Line 1:
		+
	==Methyltransferase==		==Methyltransferase==
	<StructureSection load='3rod' size='340' side='right' caption='[[3rod]], [[Resolution\|resolution]] 2.72Å' scene=''>		<StructureSection load='3rod' size='340' side='right' caption='[[3rod]], [[Resolution\|resolution]] 2.72Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rod]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ROD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ROD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rod]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ROD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ROD FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NCA:NICOTINAMIDE'>NCA</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NCA:NICOTINAMIDE'>NCA</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NNMT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NNMT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nicotinamide_N-methyltransferase Nicotinamide N-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.1 2.1.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nicotinamide_N-methyltransferase Nicotinamide N-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.1 2.1.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rod OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rod RCSB], [http://www.ebi.ac.uk/pdbsum/3rod PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rod OCA], [http://pdbe.org/3rod PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3rod RCSB], [http://www.ebi.ac.uk/pdbsum/3rod PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3rod ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3rod" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Nicotinamide N-methyltransferase]]		[[Category: Nicotinamide N-methyltransferase]]
	[[Category: Peng, Y]]		[[Category: Peng, Y]]

OCA at 22:24, 25 December 2014

OCA — Thu, 25 Dec 2014 22:24:01 GMT

←Older revision		Revision as of 22:24, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3rod]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ROD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ROD FirstGlance]. <br>		<table><tr><td colspan='2'>[[3rod]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ROD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ROD FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NCA:NICOTINAMIDE'>NCA</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NCA:NICOTINAMIDE'>NCA</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NNMT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NNMT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nicotinamide_N-methyltransferase Nicotinamide N-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.1 2.1.1.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nicotinamide_N-methyltransferase Nicotinamide N-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.1 2.1.1.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rod OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rod RCSB], [http://www.ebi.ac.uk/pdbsum/3rod PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rod OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rod RCSB], [http://www.ebi.ac.uk/pdbsum/3rod PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NNMT_HUMAN NNMT_HUMAN]] Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 16:
	Structural Basis of Substrate Recognition in Human Nicotinamide N-Methyltransferase.,Peng Y, Sartini D, Pozzi V, Wilk D, Emanuelli M, Yee VC Biochemistry. 2011 Aug 17. PMID:21823666<ref>PMID:21823666</ref>		Structural Basis of Substrate Recognition in Human Nicotinamide N-Methyltransferase.,Peng Y, Sartini D, Pozzi V, Wilk D, Emanuelli M, Yee VC Biochemistry. 2011 Aug 17. PMID:21823666<ref>PMID:21823666</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 22:		Line 24:
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Nicotinamide N-methyltransferase]]		[[Category: Nicotinamide N-methyltransferase]]
-	[[Category: Peng, Y.]]	+	[[Category: Peng, Y]]
-	[[Category: Yee, V C.]]	+	[[Category: Yee, V C]]
	[[Category: Methyltransferase]]		[[Category: Methyltransferase]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 04:42, 5 June 2014

OCA — Thu, 05 Jun 2014 04:42:02 GMT

←Older revision		Revision as of 04:42, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3rod.~~png~~\|~~left~~\|~~200px~~]]	+	==Methyltransferase==
		+	<StructureSection load='3rod' size='340' side='right' caption='[[3rod]], [[Resolution\|resolution]] 2.72Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3rod]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ROD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ROD FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NCA:NICOTINAMIDE'>NCA</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NNMT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nicotinamide_N-methyltransferase Nicotinamide N-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.1 2.1.1.1] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rod OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rod RCSB], [http://www.ebi.ac.uk/pdbsum/3rod PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Nicotinamide N-methyltransferase (NNMT) catalyzes the N-methylation of nicotinamide, pyridines, and other analogues using S-adenosyl-l-methionine as donor. NNMT plays a significant role in the regulation of metabolic pathways and is expressed at markedly high levels in several kinds of cancers, presenting it as a potential molecular target for cancer therapy. We have determined the crystal structure of human NNMT as a ternary complex bound to both the demethylated donor S-adenosyl-l-homocysteine and the acceptor substrate nicotinamide, to 2.7 A resolution. These studies reveal the structural basis for nicotinamide binding and highlight several residues in the active site which may play roles in nicotinamide recognition and NNMT catalysis. The functional importance of these residues was probed by mutagenesis. Of three residues near the nicotinamide's amide group, substitution of S201 and S213 had no effect on enzyme activity while replacement of D197 dramatically decreased activity. Substitutions of Y20, whose side chain hydroxyl interacts with both the nicotinamide aromatic ring and AdoHcy carboxylate, also compromised activity. Enzyme kinetics analysis revealed k(cat)/K(m) decreases of 2-3 orders of magnitude for the D197A and Y20A mutants, confirming the functional importance of these active site residues. The mutants exhibited substantially increased K(m) for both NCA and AdoMet and modestly decreased k(cat). MD simulations revealed long-range conformational effects which provide an explanation for the large increase in K(m)(AdoMet) for the D197A mutant, which interacts directly only with nicotinamide in the ternary complex crystal structure.

-	<!--	+	Structural Basis of Substrate Recognition in Human Nicotinamide N-Methyltransferase.,Peng Y, Sartini D, Pozzi V, Wilk D, Emanuelli M, Yee VC Biochemistry. 2011 Aug 17. PMID:21823666<ref>PMID:21823666</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3rod"~~, ~~creates the "Structure Box" on the page.~~	+
-	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded)~~,	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3rod\| PDB=3rod \| SCENE= }}~~	+

-	~~===Methyltransferase===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph~~, ~~{{ABSTRACT_PUBMED_21823666}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 21823666 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_21823666}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3rod]] is a 4 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ROD OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:021823666<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Nicotinamide N-methyltransferase]]		[[Category: Nicotinamide N-methyltransferase]]

OCA at 05:02, 21 September 2011

OCA — Wed, 21 Sep 2011 05:02:23 GMT

←Older revision		Revision as of 05:02, 21 September 2011
Line 1:		Line 1:
-	[[Image:3rod.~~jpg~~\|left\|200px]]	+	[[Image:3rod.png\|left\|200px]]

	<!--		<!--

OCA at 06:11, 7 September 2011

OCA — Wed, 07 Sep 2011 06:11:00 GMT

←Older revision		Revision as of 06:11, 7 September 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3rod.jpg\|left\|200px]]

-	The ~~entry 3rod~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3rod", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3rod\| PDB=3rod \| SCENE= }}

-	~~Authors: Peng, Y., Yee, V.C.~~	+	===Methyltransferase===

-	~~Description~~: Methyltransferase	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21823666}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21823666 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21823666}}
		+
		+	==About this Structure==
		+	[[3rod]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ROD OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021823666</ref><references group="xtra"/>
		+	[[Category: Homo sapiens]]
		+	[[Category: Nicotinamide N-methyltransferase]]
		+	[[Category: Peng, Y.]]
		+	[[Category: Yee, V C.]]
		+	[[Category: Methyltransferase]]
		+	[[Category: Transferase]]

OCA at 05:39, 29 June 2011

OCA — Wed, 29 Jun 2011 05:39:35 GMT

←Older revision		Revision as of 05:39, 29 June 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3rod is ON HOLD	+	The entry 3rod is ON HOLD until Paper Publication

	Authors: Peng, Y., Yee, V.C.		Authors: Peng, Y., Yee, V.C.

	Description: Methyltransferase		Description: Methyltransferase

OCA: Protected "3rod" [edit=sysop:move=sysop]

OCA — Wed, 04 May 2011 06:34:23 GMT

Protected "3rod" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:34, 4 May 2011

OCA: New page: '''Unreleased structure''' The entry 3rod is ON HOLD Authors: Peng, Y., Yee, V.C. Description: Methyltransferase

OCA — Wed, 04 May 2011 06:34:22 GMT

New page: '''Unreleased structure''' The entry 3rod is ON HOLD Authors: Peng, Y., Yee, V.C. Description: Methyltransferase

New page

'''Unreleased structure'''

The entry 3rod is ON HOLD

Authors: Peng, Y., Yee, V.C.

Description: Methyltransferase