3rp8 - Revision history

OCA at 09:44, 1 March 2024

OCA — Fri, 01 Mar 2024 09:44:22 GMT

←Older revision		Revision as of 09:44, 1 March 2024
Line 3:		Line 3:
	<StructureSection load='3rp8' size='340' side='right'caption='[[3rp8]], [[Resolution\|resolution]] 1.97Å' scene=''>		<StructureSection load='3rp8' size='340' side='right'caption='[[3rp8]], [[Resolution\|resolution]] 1.97Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rp8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Klep7 Klep7~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RP8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rp8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae_subsp._pneumoniae_MGH_78578 Klebsiella pneumoniae subsp. pneumoniae MGH 78578]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RP8 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>~~, ~~<scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.968Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[3rp6\|3rp6]], [[3rp7\|3rp7]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">KPN78578_16330, KPN_01663 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272620 KLEP7])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rp8 OCA], [https://pdbe.org/3rp8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rp8 RCSB], [https://www.ebi.ac.uk/pdbsum/3rp8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rp8 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rp8 OCA], [https://pdbe.org/3rp8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rp8 RCSB], [https://www.ebi.ac.uk/pdbsum/3rp8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rp8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/HPXO_KLEP7 HPXO_KLEP7]] Catalyzes the hydroxylation of uric acid to 5-hydroxyisourate.<ref>PMID:19260710</ref>	+	[https://www.uniprot.org/uniprot/HPXO_KLEP7 HPXO_KLEP7] Catalyzes the hydroxylation of uric acid to 5-hydroxyisourate.<ref>PMID:19260710</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	HpxO is a flavin-dependent urate oxidase that catalyzes the hydroxylation of uric acid to 5-hydroxyisourate and functions in a novel pathway for purine catabolism found in Klebsiella pneumoniae. We have determined the structures of HpxO with and without uric acid at 2.0 A and 2.2 A, respectively. We have also determined the structure of the R204Q mutant at 2.0 A resolution in the absence of uric acid. The mutant structure is very similar to wild type HpxO except for the conformation of Arg103, which interacts with FAD in the mutant but not in the wild type structure. Interestingly, the R204Q mutation results in the uncoupling of NADH oxidation from uric acid hydroxylation. This suggests that Arg204 facilitates the deprotonation of uric acid activating it for the oxygen transfer. Based on these data, a mechanism for this reaction is proposed consisting of a nucleophilic attack of the urate anion on the flavin hydroperoxide resulting in the formation of 5-hydroxyisourate.	+
-		+
-	Structural and Mechanistic Studies of HpxO, a Novel FAD-dependent Urate Oxidase from Klebsiella pneumoniae.,Hicks KA, O'Leary SE, Begley TP, Ealick SE Biochemistry. 2012 Dec 21. PMID:23259842<ref>PMID:23259842</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3rp8" style="background-color:#fffaf0;"></div~~>	+

	==See Also==		==See Also==
Line 27:		Line 17:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Klep7~~]]	+	[[Category: Klebsiella pneumoniae subsp. pneumoniae MGH 78578]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Begley~~, T P~~]]	+	[[Category: Begley TP]]
-	[[Category: Ealick~~, S E~~]]	+	[[Category: Ealick SE]]
-	[[Category: Hicks~~, K A~~]]	+	[[Category: Hicks KA]]
-	[[Category: Leary~~, S E.O]]~~	+	[[Category: O'Leary SE]]
-	~~[[Category: Fad-binding protein]]~~	+
-	~~[[Category: Monooxygenase]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 10:20, 22 June 2022

OCA — Wed, 22 Jun 2022 10:20:23 GMT

←Older revision		Revision as of 10:20, 22 June 2022
Line 1:		Line 1:

	==Crystal Structure of Klebsiella pneumoniae R204Q HpxO complexed with FAD==		==Crystal Structure of Klebsiella pneumoniae R204Q HpxO complexed with FAD==
-	<StructureSection load='3rp8' size='340' side='right' caption='[[3rp8]], [[Resolution\|resolution]] 1.97Å' scene=''>	+	<StructureSection load='3rp8' size='340' side='right'caption='[[3rp8]], [[Resolution\|resolution]] 1.97Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rp8]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Klep7 Klep7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RP8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3RP8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rp8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Klep7 Klep7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RP8 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rp6\|3rp6]], [[3rp7\|3rp7]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3rp6\|3rp6]], [[3rp7\|3rp7]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KPN78578_16330, KPN_01663 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272620 KLEP7])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KPN78578_16330, KPN_01663 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272620 KLEP7])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3rp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rp8 OCA], [~~http~~://pdbe.org/3rp8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3rp8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3rp8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3rp8 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rp8 OCA], [https://pdbe.org/3rp8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rp8 RCSB], [https://www.ebi.ac.uk/pdbsum/3rp8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rp8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/HPXO_KLEP7 HPXO_KLEP7]] Catalyzes the hydroxylation of uric acid to 5-hydroxyisourate.<ref>PMID:19260710</ref>	+	[[https://www.uniprot.org/uniprot/HPXO_KLEP7 HPXO_KLEP7]] Catalyzes the hydroxylation of uric acid to 5-hydroxyisourate.<ref>PMID:19260710</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 3rp8" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3rp8" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Monooxygenase 3D structures\|Monooxygenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 25:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Klep7]]		[[Category: Klep7]]
		+	[[Category: Large Structures]]
	[[Category: Begley, T P]]		[[Category: Begley, T P]]
	[[Category: Ealick, S E]]		[[Category: Ealick, S E]]

OCA at 15:53, 5 August 2016

OCA — Fri, 05 Aug 2016 15:53:55 GMT

←Older revision		Revision as of 15:53, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of Klebsiella pneumoniae R204Q HpxO complexed with FAD==		==Crystal Structure of Klebsiella pneumoniae R204Q HpxO complexed with FAD==
	<StructureSection load='3rp8' size='340' side='right' caption='[[3rp8]], [[Resolution\|resolution]] 1.97Å' scene=''>		<StructureSection load='3rp8' size='340' side='right' caption='[[3rp8]], [[Resolution\|resolution]] 1.97Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rp8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Klebsiella_pneumoniae_subsp._pneumoniae_mgh_78578 Klebsiella pneumoniae subsp. pneumoniae mgh 78578~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RP8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RP8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rp8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Klep7 Klep7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RP8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RP8 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rp6\|3rp6]], [[3rp7\|3rp7]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rp6\|3rp6]], [[3rp7\|3rp7]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KPN78578_16330, KPN_01663 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272620 ~~Klebsiella pneumoniae subsp. pneumoniae MGH 78578~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KPN78578_16330, KPN_01663 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272620 KLEP7])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rp8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rp8 RCSB], [http://www.ebi.ac.uk/pdbsum/3rp8 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rp8 OCA], [http://pdbe.org/3rp8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3rp8 RCSB], [http://www.ebi.ac.uk/pdbsum/3rp8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3rp8 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~A6T923_KLEP7 A6T923_KLEP7~~]] Catalyzes the hydroxylation of uric acid to 5-hydroxyisourate.<ref>PMID:19260710</ref>	+	[[http://www.uniprot.org/uniprot/HPXO_KLEP7 HPXO_KLEP7]] Catalyzes the hydroxylation of uric acid to 5-hydroxyisourate.<ref>PMID:19260710</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3rp8" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Klebsiella pneumoniae subsp. pneumoniae mgh 78578~~]]	+	[[Category: Klep7]]
	[[Category: Begley, T P]]		[[Category: Begley, T P]]
	[[Category: Ealick, S E]]		[[Category: Ealick, S E]]

OCA at 20:03, 25 December 2014

OCA — Thu, 25 Dec 2014 20:03:37 GMT

←Older revision		Revision as of 20:03, 25 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rp8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rp8 RCSB], [http://www.ebi.ac.uk/pdbsum/3rp8 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rp8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rp8 RCSB], [http://www.ebi.ac.uk/pdbsum/3rp8 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/A6T923_KLEP7 A6T923_KLEP7]] Catalyzes the hydroxylation of uric acid to 5-hydroxyisourate.<ref>PMID:19260710</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 11:00, 9 December 2014

OCA — Tue, 09 Dec 2014 11:00:40 GMT

←Older revision		Revision as of 11:00, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3rp8.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal Structure of Klebsiella pneumoniae R204Q HpxO complexed with FAD==
		+	<StructureSection load='3rp8' size='340' side='right' caption='[[3rp8]], [[Resolution\|resolution]] 1.97Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3rp8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae_subsp._pneumoniae_mgh_78578 Klebsiella pneumoniae subsp. pneumoniae mgh 78578]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RP8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RP8 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rp6\|3rp6]], [[3rp7\|3rp7]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KPN78578_16330, KPN_01663 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272620 Klebsiella pneumoniae subsp. pneumoniae MGH 78578])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rp8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rp8 RCSB], [http://www.ebi.ac.uk/pdbsum/3rp8 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	HpxO is a flavin-dependent urate oxidase that catalyzes the hydroxylation of uric acid to 5-hydroxyisourate and functions in a novel pathway for purine catabolism found in Klebsiella pneumoniae. We have determined the structures of HpxO with and without uric acid at 2.0 A and 2.2 A, respectively. We have also determined the structure of the R204Q mutant at 2.0 A resolution in the absence of uric acid. The mutant structure is very similar to wild type HpxO except for the conformation of Arg103, which interacts with FAD in the mutant but not in the wild type structure. Interestingly, the R204Q mutation results in the uncoupling of NADH oxidation from uric acid hydroxylation. This suggests that Arg204 facilitates the deprotonation of uric acid activating it for the oxygen transfer. Based on these data, a mechanism for this reaction is proposed consisting of a nucleophilic attack of the urate anion on the flavin hydroperoxide resulting in the formation of 5-hydroxyisourate.

-	~~{{STRUCTURE_3rp8\| PDB=3rp8 \| SCENE= }}~~	+	Structural and Mechanistic Studies of HpxO, a Novel FAD-dependent Urate Oxidase from Klebsiella pneumoniae.,Hicks KA, O'Leary SE, Begley TP, Ealick SE Biochemistry. 2012 Dec 21. PMID:23259842<ref>PMID:23259842</ref>

-	~~===Crystal Structure~~ of ~~Klebsiella pneumoniae R204Q HpxO complexed with FAD===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_23259842}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[3rp8]] is a 1 chain structure with sequence from [http:~~//en.wikipedia.org/wiki/Klebsiella_pneumoniae_subsp._pneumoniae_mgh_78578 Klebsiella pneumoniae subsp. pneumoniae mgh 78578]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RP8 OCA].	+	</StructureSection>
	[[Category: Klebsiella pneumoniae subsp. pneumoniae mgh 78578]]		[[Category: Klebsiella pneumoniae subsp. pneumoniae mgh 78578]]
-	[[Category: Begley, T P.]]	+	[[Category: Begley, T P]]
-	[[Category: Ealick, S E.]]	+	[[Category: Ealick, S E]]
-	[[Category: Hicks, K A.]]	+	[[Category: Hicks, K A]]
-	[[Category: Leary, S E.O.]]	+	[[Category: Leary, S E.O]]
	[[Category: Fad-binding protein]]		[[Category: Fad-binding protein]]
	[[Category: Monooxygenase]]		[[Category: Monooxygenase]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 08:16, 9 January 2013

OCA — Wed, 09 Jan 2013 08:16:54 GMT

←Older revision		Revision as of 08:16, 9 January 2013
Line 1:		Line 1:
-	[[Image:3rp8.~~jpg~~\|left\|200px]]	+	[[Image:3rp8.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3rp8", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3rp8\| PDB=3rp8 \| SCENE= }}		{{STRUCTURE_3rp8\| PDB=3rp8 \| SCENE= }}

	===Crystal Structure of Klebsiella pneumoniae R204Q HpxO complexed with FAD===		===Crystal Structure of Klebsiella pneumoniae R204Q HpxO complexed with FAD===

		+	{{ABSTRACT_PUBMED_23259842}}

	==About this Structure==		==About this Structure==
-	[[3rp8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Klebsiella_pneumoniae~~ Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RP8 OCA].	+	[[3rp8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae_subsp._pneumoniae_mgh_78578 Klebsiella pneumoniae subsp. pneumoniae mgh 78578]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RP8 OCA].
-	[[Category: Klebsiella pneumoniae]]	+	[[Category: Klebsiella pneumoniae subsp. pneumoniae mgh 78578]]
	[[Category: Begley, T P.]]		[[Category: Begley, T P.]]
	[[Category: Ealick, S E.]]		[[Category: Ealick, S E.]]

OCA at 09:18, 16 May 2012

OCA — Wed, 16 May 2012 09:18:09 GMT

←Older revision		Revision as of 09:18, 16 May 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3rp8.jpg\|left\|200px]]

-	The ~~entry 3rp8~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3rp8", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3rp8\| PDB=3rp8 \| SCENE= }}

-	~~Authors: Hicks, K.A., O'Leary, S.E., Begley, T.P., Ealick, S.E.~~	+	===Crystal Structure of Klebsiella pneumoniae R204Q HpxO complexed with FAD===

-	~~Description: Crystal~~ Structure of Klebsiella pneumoniae ~~R204Q HpxO complexed with FAD~~	+
		+	==About this Structure==
		+	[[3rp8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RP8 OCA].
		+	[[Category: Klebsiella pneumoniae]]
		+	[[Category: Begley, T P.]]
		+	[[Category: Ealick, S E.]]
		+	[[Category: Hicks, K A.]]
		+	[[Category: Leary, S E.O.]]
		+	[[Category: Fad-binding protein]]
		+	[[Category: Monooxygenase]]
		+	[[Category: Oxidoreductase]]

OCA at 05:14, 18 May 2011

OCA — Wed, 18 May 2011 05:14:27 GMT

←Older revision		Revision as of 05:14, 18 May 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3rp8 is ON HOLD	+	The entry 3rp8 is ON HOLD until Paper Publication

	Authors: Hicks, K.A., O'Leary, S.E., Begley, T.P., Ealick, S.E.		Authors: Hicks, K.A., O'Leary, S.E., Begley, T.P., Ealick, S.E.

	Description: Crystal Structure of Klebsiella pneumoniae R204Q HpxO complexed with FAD		Description: Crystal Structure of Klebsiella pneumoniae R204Q HpxO complexed with FAD

OCA: Protected "3rp8" [edit=sysop:move=sysop]

OCA — Wed, 04 May 2011 06:35:15 GMT

Protected "3rp8" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:35, 4 May 2011

OCA: New page: '''Unreleased structure''' The entry 3rp8 is ON HOLD Authors: Hicks, K.A., O'Leary, S.E., Begley, T.P., Ealick, S.E. Description: Crystal Structure of Klebsiella pneumoniae R204Q HpxO ...

OCA — Wed, 04 May 2011 06:35:14 GMT

New page: '''Unreleased structure''' The entry 3rp8 is ON HOLD Authors: Hicks, K.A., O'Leary, S.E., Begley, T.P., Ealick, S.E. Description: Crystal Structure of Klebsiella pneumoniae R204Q HpxO ...

New page

'''Unreleased structure'''

The entry 3rp8 is ON HOLD

Authors: Hicks, K.A., O'Leary, S.E., Begley, T.P., Ealick, S.E.

Description: Crystal Structure of Klebsiella pneumoniae R204Q HpxO complexed with FAD