3rqe - Revision history

OCA at 12:33, 14 March 2024

OCA — Thu, 14 Mar 2024 12:33:37 GMT

←Older revision		Revision as of 12:33, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3rqe' size='340' side='right'caption='[[3rqe]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='3rqe' size='340' side='right'caption='[[3rqe]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rqe]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RQE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RQE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rqe]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RQE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RQE FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[3l8i\|3l8i]], [[3l8j\|3l8j]], [[3ajm\|3ajm]], [[3rqf\|3rqf]], [[3rqg\|3rqg]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">PDCD10, CCM3, TFAR15 ([https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=9606 HUMAN])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rqe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rqe OCA], [https://pdbe.org/3rqe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rqe RCSB], [https://www.ebi.ac.uk/pdbsum/3rqe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rqe ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rqe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rqe OCA], [https://pdbe.org/3rqe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rqe RCSB], [https://www.ebi.ac.uk/pdbsum/3rqe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rqe ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[[https://www.uniprot.org/uniprot/PDC10_HUMAN PDC10_HUMAN]] Hereditary cerebral cavernous malformation. Defects in PDCD10 are the cause of cerebral cavernous malformations type 3 (CCM3) [MIM:[https://omim.org/entry/603285 603285]]. Cerebral cavernous malformations (CCMs) are congenital vascular anomalies of the central nervous system that can result in hemorrhagic stroke, seizures, recurrent headaches, and focal neurologic deficits. CCMs have an incidence of 0.1%-0.5% in the general population and usually present clinically during the 3rd to 5th decade of life. The lesions are characterized by grossly enlarged blood vessels consisting of a single layer of endothelium and without any intervening neural tissue, ranging in diameter from a few millimeters to several centimeters.<ref>PMID:15543491</ref>	+	[https://www.uniprot.org/uniprot/PDC10_HUMAN PDC10_HUMAN] Hereditary cerebral cavernous malformation. Defects in PDCD10 are the cause of cerebral cavernous malformations type 3 (CCM3) [MIM:[https://omim.org/entry/603285 603285]. Cerebral cavernous malformations (CCMs) are congenital vascular anomalies of the central nervous system that can result in hemorrhagic stroke, seizures, recurrent headaches, and focal neurologic deficits. CCMs have an incidence of 0.1%-0.5% in the general population and usually present clinically during the 3rd to 5th decade of life. The lesions are characterized by grossly enlarged blood vessels consisting of a single layer of endothelium and without any intervening neural tissue, ranging in diameter from a few millimeters to several centimeters.<ref>PMID:15543491</ref>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/PDC10_HUMAN PDC10_HUMAN]] Promotes cell proliferation. Modulates apoptotic pathways. Increases mitogen-activated protein kinase activity and MST4 activity. Important for cell migration, and for normal structure and assembly of the Golgi complex. Important for KDR/VEGFR2 signaling. Increases the stability of KDR/VEGFR2 and prevents its breakdown. Required for normal cardiovascular development. Required for normal angiogenesis, vasculogenesis and hematopoiesis during embryonic development (By similarity).<ref>PMID:15543491</ref> <ref>PMID:17360971</ref> <ref>PMID:20332113</ref>	+	[https://www.uniprot.org/uniprot/PDC10_HUMAN PDC10_HUMAN] Promotes cell proliferation. Modulates apoptotic pathways. Increases mitogen-activated protein kinase activity and MST4 activity. Important for cell migration, and for normal structure and assembly of the Golgi complex. Important for KDR/VEGFR2 signaling. Increases the stability of KDR/VEGFR2 and prevents its breakdown. Required for normal cardiovascular development. Required for normal angiogenesis, vasculogenesis and hematopoiesis during embryonic development (By similarity).<ref>PMID:15543491</ref> <ref>PMID:17360971</ref> <ref>PMID:20332113</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Cerebral cavernous malformation (CCM) is a disease that affects between 0.1 and 0.5% of the human population, with mutations in CCM3 accounting for approximately 15% of the autosomal dominant form of the disease. We recently reported that CCM3 contains an N-terminal dimerization domain (CCM3D) and a C-terminal focal adhesion targeting (FAT)-homology domain. Intermolecular protein-protein interactions of CCM3 are mediated by a highly conserved surface on the FAT-homology domain and are affected by CCM3 truncations in the human disease. Here we report the crystal structures of CCM3 in complex with three different leucine-aspartate repeat (LD) motifs (LD1, LD2 and LD4) from the scaffolding protein paxillin, at 2.8 A, 2.7 A and 2.5 A resolution. We show that CCM3 binds LD motifs using the highly conserved Hydrophobic Patch 1 (HP1) and that this binding is similar to the binding of FAK and Pyk2 FAT domains to paxillin LD motifs. We further show by Surface Plasmon Resonance that CCM3 binds paxillin LD motifs with affinities in the micromolar range, similar to FAK family FAT domains. Finally, we show that endogenous CCM3 and paxillin co-localize in mouse cerebral pericytes. These studies provide a molecular-level framework to investigate the protein-protein interactions of CCM3.	+
-		+
-	Molecular recognition of leucine-aspartate repeat (LD) motifs by the focal adhesion targeting-homology domain of cerebral cavernous malformation 3 (CCM3).,Li X, Ji W, Zhang R, Folta-Stogniew E, Min W, Boggon TJ J Biol Chem. 2011 Jun 1. PMID:21632544<ref>PMID:21632544</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3rqe" style="background-color:#fffaf0;"></div~~>	+

	==See Also==		==See Also==
Line 29:		Line 19:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Boggon~~, T J~~]]	+	[[Category: Boggon TJ]]
-	[[Category: Li, X]]	+	[[Category: Li X]]
-	[[Category: Zhang, R]]	+	[[Category: Zhang R]]
-	~~[[Category: Cerebral cavernous malformation]]~~	+
-	~~[[Category: Dimerization]]~~	+
-	~~[[Category: Fat domain]]~~	+
-	~~[[Category: Protein binding]]~~	+
-	~~[[Category: Protein-peptide complex~~]]	+

OCA at 10:21, 22 June 2022

OCA — Wed, 22 Jun 2022 10:21:46 GMT

←Older revision		Revision as of 10:21, 22 June 2022
Line 1:		Line 1:

	==Cerebral cavernous malformation 3 (CCM3) in complex with paxillin LD1==		==Cerebral cavernous malformation 3 (CCM3) in complex with paxillin LD1==
-	<StructureSection load='3rqe' size='340' side='right' caption='[[3rqe]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='3rqe' size='340' side='right'caption='[[3rqe]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rqe]] is a 5 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RQE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3RQE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rqe]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RQE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RQE FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3l8i\|3l8i]], [[3l8j\|3l8j]], [[3ajm\|3ajm]], [[3rqf\|3rqf]], [[3rqg\|3rqg]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3l8i\|3l8i]], [[3l8j\|3l8j]], [[3ajm\|3ajm]], [[3rqf\|3rqf]], [[3rqg\|3rqg]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PDCD10, CCM3, TFAR15 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PDCD10, CCM3, TFAR15 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3rqe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rqe OCA], [~~http~~://pdbe.org/3rqe PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3rqe RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3rqe PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3rqe ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rqe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rqe OCA], [https://pdbe.org/3rqe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rqe RCSB], [https://www.ebi.ac.uk/pdbsum/3rqe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rqe ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[[~~http~~://www.uniprot.org/uniprot/PDC10_HUMAN PDC10_HUMAN]] Hereditary cerebral cavernous malformation. Defects in PDCD10 are the cause of cerebral cavernous malformations type 3 (CCM3) [MIM:[~~http~~://omim.org/entry/603285 603285]]. Cerebral cavernous malformations (CCMs) are congenital vascular anomalies of the central nervous system that can result in hemorrhagic stroke, seizures, recurrent headaches, and focal neurologic deficits. CCMs have an incidence of 0.1%-0.5% in the general population and usually present clinically during the 3rd to 5th decade of life. The lesions are characterized by grossly enlarged blood vessels consisting of a single layer of endothelium and without any intervening neural tissue, ranging in diameter from a few millimeters to several centimeters.<ref>PMID:15543491</ref>	+	[[https://www.uniprot.org/uniprot/PDC10_HUMAN PDC10_HUMAN]] Hereditary cerebral cavernous malformation. Defects in PDCD10 are the cause of cerebral cavernous malformations type 3 (CCM3) [MIM:[https://omim.org/entry/603285 603285]]. Cerebral cavernous malformations (CCMs) are congenital vascular anomalies of the central nervous system that can result in hemorrhagic stroke, seizures, recurrent headaches, and focal neurologic deficits. CCMs have an incidence of 0.1%-0.5% in the general population and usually present clinically during the 3rd to 5th decade of life. The lesions are characterized by grossly enlarged blood vessels consisting of a single layer of endothelium and without any intervening neural tissue, ranging in diameter from a few millimeters to several centimeters.<ref>PMID:15543491</ref>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PDC10_HUMAN PDC10_HUMAN]] Promotes cell proliferation. Modulates apoptotic pathways. Increases mitogen-activated protein kinase activity and MST4 activity. Important for cell migration, and for normal structure and assembly of the Golgi complex. Important for KDR/VEGFR2 signaling. Increases the stability of KDR/VEGFR2 and prevents its breakdown. Required for normal cardiovascular development. Required for normal angiogenesis, vasculogenesis and hematopoiesis during embryonic development (By similarity).<ref>PMID:15543491</ref> <ref>PMID:17360971</ref> <ref>PMID:20332113</ref>	+	[[https://www.uniprot.org/uniprot/PDC10_HUMAN PDC10_HUMAN]] Promotes cell proliferation. Modulates apoptotic pathways. Increases mitogen-activated protein kinase activity and MST4 activity. Important for cell migration, and for normal structure and assembly of the Golgi complex. Important for KDR/VEGFR2 signaling. Increases the stability of KDR/VEGFR2 and prevents its breakdown. Required for normal cardiovascular development. Required for normal angiogenesis, vasculogenesis and hematopoiesis during embryonic development (By similarity).<ref>PMID:15543491</ref> <ref>PMID:17360971</ref> <ref>PMID:20332113</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:

	==See Also==		==See Also==
-	*[[Cell death protein\|Cell death protein]]	+	*[[Cell death protein 3D structures\|Cell death protein 3D structures]]
	*[[Paxillin\|Paxillin]]		*[[Paxillin\|Paxillin]]
	== References ==		== References ==
Line 30:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Boggon, T J]]		[[Category: Boggon, T J]]
	[[Category: Li, X]]		[[Category: Li, X]]

OCA at 08:57, 11 August 2016

OCA — Thu, 11 Aug 2016 08:57:04 GMT

←Older revision		Revision as of 08:57, 11 August 2016
Line 1:		Line 1:
		+
	==Cerebral cavernous malformation 3 (CCM3) in complex with paxillin LD1==		==Cerebral cavernous malformation 3 (CCM3) in complex with paxillin LD1==
	<StructureSection load='3rqe' size='340' side='right' caption='[[3rqe]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='3rqe' size='340' side='right' caption='[[3rqe]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rqe]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RQE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RQE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rqe]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RQE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RQE FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3l8i\|3l8i]], [[3l8j\|3l8j]], [[3ajm\|3ajm]], [[3rqf\|3rqf]], [[3rqg\|3rqg]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3l8i\|3l8i]], [[3l8j\|3l8j]], [[3ajm\|3ajm]], [[3rqf\|3rqf]], [[3rqg\|3rqg]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PDCD10, CCM3, TFAR15 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PDCD10, CCM3, TFAR15 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rqe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rqe OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rqe RCSB], [http://www.ebi.ac.uk/pdbsum/3rqe PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rqe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rqe OCA], [http://pdbe.org/3rqe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3rqe RCSB], [http://www.ebi.ac.uk/pdbsum/3rqe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3rqe ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 19:		Line 20:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3rqe" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Cell death protein\|Cell death protein]]		*[[Cell death protein\|Cell death protein]]
		+	*[[Paxillin\|Paxillin]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Boggon, T J]]		[[Category: Boggon, T J]]
	[[Category: Li, X]]		[[Category: Li, X]]

OCA at 09:04, 4 January 2015

OCA — Sun, 04 Jan 2015 09:04:26 GMT

←Older revision		Revision as of 09:04, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3rqe]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RQE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RQE FirstGlance]. <br>		<table><tr><td colspan='2'>[[3rqe]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RQE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RQE FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3l8i\|3l8i]], [[3l8j\|3l8j]], [[3ajm\|3ajm]], [[3rqf\|3rqf]], [[3rqg\|3rqg]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3l8i\|3l8i]], [[3l8j\|3l8j]], [[3ajm\|3ajm]], [[3rqf\|3rqf]], [[3rqg\|3rqg]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PDCD10, CCM3, TFAR15 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PDCD10, CCM3, TFAR15 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rqe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rqe OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rqe RCSB], [http://www.ebi.ac.uk/pdbsum/3rqe PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rqe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rqe OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rqe RCSB], [http://www.ebi.ac.uk/pdbsum/3rqe PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Disease ==		== Disease ==
	[[http://www.uniprot.org/uniprot/PDC10_HUMAN PDC10_HUMAN]] Hereditary cerebral cavernous malformation. Defects in PDCD10 are the cause of cerebral cavernous malformations type 3 (CCM3) [MIM:[http://omim.org/entry/603285 603285]]. Cerebral cavernous malformations (CCMs) are congenital vascular anomalies of the central nervous system that can result in hemorrhagic stroke, seizures, recurrent headaches, and focal neurologic deficits. CCMs have an incidence of 0.1%-0.5% in the general population and usually present clinically during the 3rd to 5th decade of life. The lesions are characterized by grossly enlarged blood vessels consisting of a single layer of endothelium and without any intervening neural tissue, ranging in diameter from a few millimeters to several centimeters.<ref>PMID:15543491</ref>		[[http://www.uniprot.org/uniprot/PDC10_HUMAN PDC10_HUMAN]] Hereditary cerebral cavernous malformation. Defects in PDCD10 are the cause of cerebral cavernous malformations type 3 (CCM3) [MIM:[http://omim.org/entry/603285 603285]]. Cerebral cavernous malformations (CCMs) are congenital vascular anomalies of the central nervous system that can result in hemorrhagic stroke, seizures, recurrent headaches, and focal neurologic deficits. CCMs have an incidence of 0.1%-0.5% in the general population and usually present clinically during the 3rd to 5th decade of life. The lesions are characterized by grossly enlarged blood vessels consisting of a single layer of endothelium and without any intervening neural tissue, ranging in diameter from a few millimeters to several centimeters.<ref>PMID:15543491</ref>
Line 17:		Line 17:
	Molecular recognition of leucine-aspartate repeat (LD) motifs by the focal adhesion targeting-homology domain of cerebral cavernous malformation 3 (CCM3).,Li X, Ji W, Zhang R, Folta-Stogniew E, Min W, Boggon TJ J Biol Chem. 2011 Jun 1. PMID:21632544<ref>PMID:21632544</ref>		Molecular recognition of leucine-aspartate repeat (LD) motifs by the focal adhesion targeting-homology domain of cerebral cavernous malformation 3 (CCM3).,Li X, Ji W, Zhang R, Folta-Stogniew E, Min W, Boggon TJ J Biol Chem. 2011 Jun 1. PMID:21632544<ref>PMID:21632544</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>

Line 27:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Boggon, T J.]]	+	[[Category: Boggon, T J]]
-	[[Category: Li, X.]]	+	[[Category: Li, X]]
-	[[Category: Zhang, R.]]	+	[[Category: Zhang, R]]
	[[Category: Cerebral cavernous malformation]]		[[Category: Cerebral cavernous malformation]]
	[[Category: Dimerization]]		[[Category: Dimerization]]

OCA at 04:38, 5 June 2014

OCA — Thu, 05 Jun 2014 04:38:15 GMT

←Older revision		Revision as of 04:38, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3rqe.~~png~~\|~~left~~\|~~200px~~]]	+	==Cerebral cavernous malformation 3 (CCM3) in complex with paxillin LD1==
		+	<StructureSection load='3rqe' size='340' side='right' caption='[[3rqe]], [[Resolution\|resolution]] 2.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3rqe]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RQE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RQE FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3l8i\|3l8i]], [[3l8j\|3l8j]], [[3ajm\|3ajm]], [[3rqf\|3rqf]], [[3rqg\|3rqg]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PDCD10, CCM3, TFAR15 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rqe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rqe OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rqe RCSB], [http://www.ebi.ac.uk/pdbsum/3rqe PDBsum]</span></td></tr>
		+	<table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/PDC10_HUMAN PDC10_HUMAN]] Hereditary cerebral cavernous malformation. Defects in PDCD10 are the cause of cerebral cavernous malformations type 3 (CCM3) [MIM:[http://omim.org/entry/603285 603285]]. Cerebral cavernous malformations (CCMs) are congenital vascular anomalies of the central nervous system that can result in hemorrhagic stroke, seizures, recurrent headaches, and focal neurologic deficits. CCMs have an incidence of 0.1%-0.5% in the general population and usually present clinically during the 3rd to 5th decade of life. The lesions are characterized by grossly enlarged blood vessels consisting of a single layer of endothelium and without any intervening neural tissue, ranging in diameter from a few millimeters to several centimeters.<ref>PMID:15543491</ref>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PDC10_HUMAN PDC10_HUMAN]] Promotes cell proliferation. Modulates apoptotic pathways. Increases mitogen-activated protein kinase activity and MST4 activity. Important for cell migration, and for normal structure and assembly of the Golgi complex. Important for KDR/VEGFR2 signaling. Increases the stability of KDR/VEGFR2 and prevents its breakdown. Required for normal cardiovascular development. Required for normal angiogenesis, vasculogenesis and hematopoiesis during embryonic development (By similarity).<ref>PMID:15543491</ref> <ref>PMID:17360971</ref> <ref>PMID:20332113</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cerebral cavernous malformation (CCM) is a disease that affects between 0.1 and 0.5% of the human population, with mutations in CCM3 accounting for approximately 15% of the autosomal dominant form of the disease. We recently reported that CCM3 contains an N-terminal dimerization domain (CCM3D) and a C-terminal focal adhesion targeting (FAT)-homology domain. Intermolecular protein-protein interactions of CCM3 are mediated by a highly conserved surface on the FAT-homology domain and are affected by CCM3 truncations in the human disease. Here we report the crystal structures of CCM3 in complex with three different leucine-aspartate repeat (LD) motifs (LD1, LD2 and LD4) from the scaffolding protein paxillin, at 2.8 A, 2.7 A and 2.5 A resolution. We show that CCM3 binds LD motifs using the highly conserved Hydrophobic Patch 1 (HP1) and that this binding is similar to the binding of FAK and Pyk2 FAT domains to paxillin LD motifs. We further show by Surface Plasmon Resonance that CCM3 binds paxillin LD motifs with affinities in the micromolar range, similar to FAK family FAT domains. Finally, we show that endogenous CCM3 and paxillin co-localize in mouse cerebral pericytes. These studies provide a molecular-level framework to investigate the protein-protein interactions of CCM3.

-	<!--	+	Molecular recognition of leucine-aspartate repeat (LD) motifs by the focal adhesion targeting-homology domain of cerebral cavernous malformation 3 (CCM3).,Li X, Ji W, Zhang R, Folta-Stogniew E, Min W, Boggon TJ J Biol Chem. 2011 Jun 1. PMID:21632544<ref>PMID:21632544</ref>
-	~~The line below this paragraph, containing "STRUCTURE_3rqe", creates the "Structure Box" on the page.~~	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	or the ~~SCENE parameter~~ (~~which sets the initial scene displayed when the page is loaded~~),	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3rqe\| PDB=3rqe \| SCENE= }}~~	+

-	~~===Cerebral cavernous malformation 3 (CCM3) in complex with paxillin LD1===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-		+	==See Also==
-	~~<!--~~	+	*[[Cell death protein\|Cell death protein]]
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_21632544}}, adds the Publication Abstract to the page~~	+	== References ==
-	~~(as it appears on PubMed at http://www.pubmed.gov), where 21632544 is the PubMed ID number.~~	+	<references/>
-	~~-->~~	+	__TOC__
-	~~{{ABSTRACT_PUBMED_21632544}}~~	+	</StructureSection>
-		+
-	==~~About this Structure~~==	+
-	[[~~3rqe~~]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RQE OCA].	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:021632544<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Boggon, T J.]]		[[Category: Boggon, T J.]]

OCA at 07:22, 13 July 2011

OCA — Wed, 13 Jul 2011 07:22:40 GMT

←Older revision		Revision as of 07:22, 13 July 2011
Line 1:		Line 1:
-	[[Image:3rqe.~~jpg~~\|left\|200px]]	+	[[Image:3rqe.png\|left\|200px]]

	<!--		<!--
Line 11:		Line 11:
	===Cerebral cavernous malformation 3 (CCM3) in complex with paxillin LD1===		===Cerebral cavernous malformation 3 (CCM3) in complex with paxillin LD1===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21632544}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21632544 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21632544}}

	==About this Structure==		==About this Structure==
	[[3rqe]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RQE OCA].		[[3rqe]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RQE OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021632544</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Boggon, T J.]]		[[Category: Boggon, T J.]]
	[[Category: Li, X.]]		[[Category: Li, X.]]
	[[Category: Zhang, R.]]		[[Category: Zhang, R.]]
		+	[[Category: Cerebral cavernous malformation]]
		+	[[Category: Dimerization]]
		+	[[Category: Fat domain]]
		+	[[Category: Protein binding]]
		+	[[Category: Protein-peptide complex]]

OCA at 06:01, 1 June 2011

OCA — Wed, 01 Jun 2011 06:01:27 GMT

←Older revision		Revision as of 06:01, 1 June 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3rqe.jpg\|left\|200px]]

-	The ~~entry 3rqe~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3rqe", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3rqe\| PDB=3rqe \| SCENE= }}

-	~~Authors: Li, X., Zhang, R., Boggon, T.J.~~	+	===Cerebral cavernous malformation 3 (CCM3) in complex with paxillin LD1===

-	~~Description: Cerebral cavernous malformation 3 (CCM3) in complex~~ with ~~paxillin LD1~~	+
		+	==About this Structure==
		+	[[3rqe]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RQE OCA].
		+	[[Category: Homo sapiens]]
		+	[[Category: Boggon, T J.]]
		+	[[Category: Li, X.]]
		+	[[Category: Zhang, R.]]

OCA: Protected "3rqe" [edit=sysop:move=sysop]

OCA — Wed, 04 May 2011 06:36:32 GMT

Protected "3rqe" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:36, 4 May 2011

OCA: New page: '''Unreleased structure''' The entry 3rqe is ON HOLD Authors: Li, X., Zhang, R., Boggon, T.J. Description: Cerebral cavernous malformation 3 (CCM3) in complex with paxillin LD1

OCA — Wed, 04 May 2011 06:36:31 GMT

New page: '''Unreleased structure''' The entry 3rqe is ON HOLD Authors: Li, X., Zhang, R., Boggon, T.J. Description: Cerebral cavernous malformation 3 (CCM3) in complex with paxillin LD1

New page

'''Unreleased structure'''

The entry 3rqe is ON HOLD

Authors: Li, X., Zhang, R., Boggon, T.J.

Description: Cerebral cavernous malformation 3 (CCM3) in complex with paxillin LD1