3rqw - Revision history

OCA at 12:34, 14 March 2024

OCA — Thu, 14 Mar 2024 12:34:22 GMT

←Older revision		Revision as of 12:34, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3rqw' size='340' side='right'caption='[[3rqw]], [[Resolution\|resolution]] 2.91Å' scene=''>		<StructureSection load='3rqw' size='340' side='right'caption='[[3rqw]], [[Resolution\|resolution]] 2.91Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rqw]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Dicd3 Dicd3~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RQW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RQW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rqw]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Dickeya_dadantii_3937 Dickeya dadantii 3937]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RQW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RQW FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ACH:ACETYLCHOLINE'>ACH</scene>~~, ~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:~~2~~-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.913Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[3rqu\|3rqu]]~~</~~div~~><~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACH:ACETYLCHOLINE'>ACH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>
-	<~~tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">Dda3937_00520 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198628 DICD3])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rqw OCA], [https://pdbe.org/3rqw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rqw RCSB], [https://www.ebi.ac.uk/pdbsum/3rqw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rqw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rqw OCA], [https://pdbe.org/3rqw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rqw RCSB], [https://www.ebi.ac.uk/pdbsum/3rqw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rqw ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/E0SJQ4_DICD3 E0SJQ4_DICD3]
-	ELIC, the pentameric ligand-gated ion channel from Erwinia chrysanthemi, is a prototype for Cys-loop receptors. Here we show that acetylcholine is a competitive antagonist for ELIC. We determine the acetylcholine-ELIC cocrystal structure to a 2.9-A resolution and find that acetylcholine binding to an aromatic cage at the subunit interface induces a significant contraction of loop C and other structural rearrangements in the extracellular domain. The side chain of the pore-lining residue F247 reorients and the pore size consequently enlarges, but the channel remains closed. We attribute the inability of acetylcholine to activate ELIC primarily to weak cation-pi and electrostatic interactions in the pocket, because an acetylcholine derivative with a simple quaternary-to-tertiary ammonium substitution activates the channel. This study presents a compelling case for understanding the structural underpinning of the functional relationship between agonism and competitive antagonism in the Cys-loop receptors, providing a new framework for developing novel therapeutic drugs.	+
-		+
-	Structure of the pentameric ligand-gated ion channel ELIC cocrystallized with its competitive antagonist acetylcholine.,Pan J, Chen Q, Willenbring D, Yoshida K, Tillman T, Kashlan OB, Cohen A, Kong XP, Xu Y, Tang P Nat Commun. 2012 Mar 6;3:~~714. doi: 10.1038~~/~~ncomms1703. PMID:22395605<ref>PMID:22395605<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3rqw" style="background-color:#fffaf0;"><~~/~~div>~~	+

	==See Also==		==See Also==
	*[[Ion channels 3D structures\|Ion channels 3D structures]]		*[[Ion channels 3D structures\|Ion channels 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Dicd3~~]]	+	[[Category: Dickeya dadantii 3937]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chen, Q]]	+	[[Category: Chen Q]]
-	[[Category: Cohen, A]]	+	[[Category: Cohen A]]
-	[[Category: Kong~~, X P~~]]	+	[[Category: Kong XP]]
-	[[Category: Pan~~, J J~~]]	+	[[Category: Pan JJ]]
-	[[Category: Tang, P]]	+	[[Category: Tang P]]
-	[[Category: Xu, Y]]	+	[[Category: Xu Y]]
-	[[Category: Yoshida, K]]	+	[[Category: Yoshida K]]
-	~~[[Category: Ion channel]]~~	+
-	~~[[Category: Membrane]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 10:22, 22 June 2022

OCA — Wed, 22 Jun 2022 10:22:17 GMT

←Older revision		Revision as of 10:22, 22 June 2022
Line 1:		Line 1:

	==Crystal structure of acetylcholine bound to a prokaryotic pentameric ligand-gated ion channel, ELIC==		==Crystal structure of acetylcholine bound to a prokaryotic pentameric ligand-gated ion channel, ELIC==
-	<StructureSection load='3rqw' size='340' side='right' caption='[[3rqw]], [[Resolution\|resolution]] 2.91Å' scene=''>	+	<StructureSection load='3rqw' size='340' side='right'caption='[[3rqw]], [[Resolution\|resolution]] 2.91Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rqw]] is a 10 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Dicd3 Dicd3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RQW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3RQW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rqw]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Dicd3 Dicd3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RQW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RQW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACH:ACETYLCHOLINE'>ACH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACH:ACETYLCHOLINE'>ACH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rqu\|3rqu]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3rqu\|3rqu]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dda3937_00520 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198628 DICD3])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dda3937_00520 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198628 DICD3])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3rqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rqw OCA], [~~http~~://pdbe.org/3rqw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3rqw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3rqw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3rqw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rqw OCA], [https://pdbe.org/3rqw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rqw RCSB], [https://www.ebi.ac.uk/pdbsum/3rqw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rqw ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 20:

	==See Also==		==See Also==
-	*[[Ion channels\|Ion channels]]	+	*[[Ion channels 3D structures\|Ion channels 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 26:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Dicd3]]		[[Category: Dicd3]]
		+	[[Category: Large Structures]]
	[[Category: Chen, Q]]		[[Category: Chen, Q]]
	[[Category: Cohen, A]]		[[Category: Cohen, A]]

OCA at 08:26, 11 August 2016

OCA — Thu, 11 Aug 2016 08:26:05 GMT

←Older revision		Revision as of 08:26, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of acetylcholine bound to a prokaryotic pentameric ligand-gated ion channel, ELIC==		==Crystal structure of acetylcholine bound to a prokaryotic pentameric ligand-gated ion channel, ELIC==
	<StructureSection load='3rqw' size='340' side='right' caption='[[3rqw]], [[Resolution\|resolution]] 2.91Å' scene=''>		<StructureSection load='3rqw' size='340' side='right' caption='[[3rqw]], [[Resolution\|resolution]] 2.91Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rqw]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Dickeya_dadantii Dickeya dadantii~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RQW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RQW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rqw]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Dicd3 Dicd3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RQW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RQW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACH:ACETYLCHOLINE'>ACH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACH:ACETYLCHOLINE'>ACH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rqu\|3rqu]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rqu\|3rqu]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dda3937_00520 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~204038 Dickeya dadantii~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dda3937_00520 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198628 DICD3])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rqw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rqw RCSB], [http://www.ebi.ac.uk/pdbsum/3rqw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rqw OCA], [http://pdbe.org/3rqw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3rqw RCSB], [http://www.ebi.ac.uk/pdbsum/3rqw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3rqw ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3rqw" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ion channels\|Ion channels]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Dickeya dadantii~~]]	+	[[Category: Dicd3]]
	[[Category: Chen, Q]]		[[Category: Chen, Q]]
	[[Category: Cohen, A]]		[[Category: Cohen, A]]

OCA at 10:35, 4 January 2015

OCA — Sun, 04 Jan 2015 10:35:19 GMT

←Older revision		Revision as of 10:35, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3rqw]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Dickeya_dadantii Dickeya dadantii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RQW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RQW FirstGlance]. <br>		<table><tr><td colspan='2'>[[3rqw]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Dickeya_dadantii Dickeya dadantii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RQW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RQW FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACH:ACETYLCHOLINE'>ACH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACH:ACETYLCHOLINE'>ACH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rqu\|3rqu]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rqu\|3rqu]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dda3937_00520 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=204038 Dickeya dadantii])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dda3937_00520 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=204038 Dickeya dadantii])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rqw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rqw RCSB], [http://www.ebi.ac.uk/pdbsum/3rqw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rqw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rqw RCSB], [http://www.ebi.ac.uk/pdbsum/3rqw PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 14:
	Structure of the pentameric ligand-gated ion channel ELIC cocrystallized with its competitive antagonist acetylcholine.,Pan J, Chen Q, Willenbring D, Yoshida K, Tillman T, Kashlan OB, Cohen A, Kong XP, Xu Y, Tang P Nat Commun. 2012 Mar 6;3:714. doi: 10.1038/ncomms1703. PMID:22395605<ref>PMID:22395605</ref>		Structure of the pentameric ligand-gated ion channel ELIC cocrystallized with its competitive antagonist acetylcholine.,Pan J, Chen Q, Willenbring D, Yoshida K, Tillman T, Kashlan OB, Cohen A, Kong XP, Xu Y, Tang P Nat Commun. 2012 Mar 6;3:714. doi: 10.1038/ncomms1703. PMID:22395605<ref>PMID:22395605</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Dickeya dadantii]]		[[Category: Dickeya dadantii]]
-	[[Category: Chen, Q.]]	+	[[Category: Chen, Q]]
-	[[Category: Cohen, A.]]	+	[[Category: Cohen, A]]
-	[[Category: Kong, X P.]]	+	[[Category: Kong, X P]]
-	[[Category: Pan, J J.]]	+	[[Category: Pan, J J]]
-	[[Category: Tang, P.]]	+	[[Category: Tang, P]]
-	[[Category: Xu, Y.]]	+	[[Category: Xu, Y]]
-	[[Category: Yoshida, K.]]	+	[[Category: Yoshida, K]]
	[[Category: Ion channel]]		[[Category: Ion channel]]
	[[Category: Membrane]]		[[Category: Membrane]]
	[[Category: Transport protein]]		[[Category: Transport protein]]

OCA at 04:52, 5 June 2014

OCA — Thu, 05 Jun 2014 04:52:44 GMT

←Older revision		Revision as of 04:52, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3rqw.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of acetylcholine bound to a prokaryotic pentameric ligand-gated ion channel, ELIC==
		+	<StructureSection load='3rqw' size='340' side='right' caption='[[3rqw]], [[Resolution\|resolution]] 2.91Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3rqw]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Dickeya_dadantii Dickeya dadantii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RQW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RQW FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACH:ACETYLCHOLINE'>ACH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3rqu\|3rqu]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dda3937_00520 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=204038 Dickeya dadantii])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rqw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rqw RCSB], [http://www.ebi.ac.uk/pdbsum/3rqw PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	ELIC, the pentameric ligand-gated ion channel from Erwinia chrysanthemi, is a prototype for Cys-loop receptors. Here we show that acetylcholine is a competitive antagonist for ELIC. We determine the acetylcholine-ELIC cocrystal structure to a 2.9-A resolution and find that acetylcholine binding to an aromatic cage at the subunit interface induces a significant contraction of loop C and other structural rearrangements in the extracellular domain. The side chain of the pore-lining residue F247 reorients and the pore size consequently enlarges, but the channel remains closed. We attribute the inability of acetylcholine to activate ELIC primarily to weak cation-pi and electrostatic interactions in the pocket, because an acetylcholine derivative with a simple quaternary-to-tertiary ammonium substitution activates the channel. This study presents a compelling case for understanding the structural underpinning of the functional relationship between agonism and competitive antagonism in the Cys-loop receptors, providing a new framework for developing novel therapeutic drugs.

-	~~<!--~~	+	Structure of the pentameric ligand-gated ion channel ELIC cocrystallized with its competitive antagonist acetylcholine.,Pan J, Chen Q, Willenbring D, Yoshida K, Tillman T, Kashlan OB, Cohen A, Kong XP, Xu Y, Tang P Nat Commun. 2012 Mar 6;3:714. doi: 10.1038/ncomms1703. PMID:22395605<ref>PMID:22395605</ref>
-	~~The line below this paragraph, containing "STRUCTURE_3rqw", creates the "~~Structure ~~Box" on~~ the ~~page~~.	+
-	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded)~~,	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3rqw\| PDB=3rqw \| SCENE= }}~~	+

-	~~===Crystal structure~~ of ~~acetylcholine bound to a prokaryotic pentameric ligand-gated ion channel, ELIC===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22395605}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22395605 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_22395605}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3rqw]] is a 10 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Dickeya_dadantii Dickeya dadantii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RQW OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022395605<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Dickeya dadantii]]		[[Category: Dickeya dadantii]]
	[[Category: Chen, Q.]]		[[Category: Chen, Q.]]

OCA at 06:56, 28 March 2012

OCA — Wed, 28 Mar 2012 06:56:28 GMT

←Older revision		Revision as of 06:56, 28 March 2012
Line 11:		Line 11:
	===Crystal structure of acetylcholine bound to a prokaryotic pentameric ligand-gated ion channel, ELIC===		===Crystal structure of acetylcholine bound to a prokaryotic pentameric ligand-gated ion channel, ELIC===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22395605}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22395605 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22395605}}

	==About this Structure==		==About this Structure==
	[[3rqw]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Dickeya_dadantii Dickeya dadantii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RQW OCA].		[[3rqw]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Dickeya_dadantii Dickeya dadantii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RQW OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022395605</ref><references group="xtra"/>
	[[Category: Dickeya dadantii]]		[[Category: Dickeya dadantii]]
	[[Category: Chen, Q.]]		[[Category: Chen, Q.]]

OCA at 05:43, 14 March 2012

OCA — Wed, 14 Mar 2012 05:43:49 GMT

←Older revision		Revision as of 05:43, 14 March 2012
Line 1:		Line 1:
-	[[Image:3rqw.~~jpg~~\|left\|200px]]	+	[[Image:3rqw.png\|left\|200px]]

	<!--		<!--
Line 21:		Line 21:
	[[Category: Tang, P.]]		[[Category: Tang, P.]]
	[[Category: Xu, Y.]]		[[Category: Xu, Y.]]
-	[[Category: ~~Yushida~~, K.]]	+	[[Category: Yoshida, K.]]
	[[Category: Ion channel]]		[[Category: Ion channel]]
	[[Category: Membrane]]		[[Category: Membrane]]
	[[Category: Transport protein]]		[[Category: Transport protein]]

OCA at 05:52, 8 March 2012

OCA — Thu, 08 Mar 2012 05:52:55 GMT

←Older revision		Revision as of 05:52, 8 March 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3rqw.jpg\|left\|200px]]

-	The ~~entry 3rqw~~ is ~~ON HOLD~~ ~~until Apr 28 2013~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3rqw", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3rqw\| PDB=3rqw \| SCENE= }}

-	~~Authors: Pan~~, ~~J.J., Chen, Q., Yushida, K., Cohen, A., Kong, X.P., Xu, Y., Tang, P.~~	+	===Crystal structure of acetylcholine bound to a prokaryotic pentameric ligand-gated ion channel, ELIC===

-	~~Description~~:	+
		+	==About this Structure==
		+	[[3rqw]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Dickeya_dadantii Dickeya dadantii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RQW OCA].
		+	[[Category: Dickeya dadantii]]
		+	[[Category: Chen, Q.]]
		+	[[Category: Cohen, A.]]
		+	[[Category: Kong, X P.]]
		+	[[Category: Pan, J J.]]
		+	[[Category: Tang, P.]]
		+	[[Category: Xu, Y.]]
		+	[[Category: Yushida, K.]]
		+	[[Category: Ion channel]]
		+	[[Category: Membrane]]
		+	[[Category: Transport protein]]

OCA at 07:14, 22 February 2012

OCA — Wed, 22 Feb 2012 07:14:40 GMT

←Older revision		Revision as of 07:14, 22 February 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3rqw is ON HOLD until ~~Paper Publication~~	+	The entry 3rqw is ON HOLD until Apr 28 2013

	Authors: Pan, J.J., Chen, Q., Yushida, K., Cohen, A., Kong, X.P., Xu, Y., Tang, P.		Authors: Pan, J.J., Chen, Q., Yushida, K., Cohen, A., Kong, X.P., Xu, Y., Tang, P.

	Description:		Description:

OCA at 05:14, 18 May 2011

OCA — Wed, 18 May 2011 05:14:51 GMT

←Older revision		Revision as of 05:14, 18 May 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3rqw is ON HOLD	+	The entry 3rqw is ON HOLD until Paper Publication

	Authors: Pan, J.J., Chen, Q., Yushida, K., Cohen, A., Kong, X.P., Xu, Y., Tang, P.		Authors: Pan, J.J., Chen, Q., Yushida, K., Cohen, A., Kong, X.P., Xu, Y., Tang, P.

	Description:		Description: