3rro - Revision history

OCA at 12:35, 14 March 2024

OCA — Thu, 14 Mar 2024 12:35:05 GMT

←Older revision		Revision as of 12:35, 14 March 2024
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/FABG_VIBCH FABG_VIBCH] Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis.		[https://www.uniprot.org/uniprot/FABG_VIBCH FABG_VIBCH] Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis.
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	beta-ketoacyl-(acyl carrier protein) reductase (FabG) catalyzes the key reductive reaction in the elongation cycle of fatty acid synthesis (FAS), which is a vital metabolic pathway in bacteria and a promising target for new antibiotic development. The activation of the enzyme is usually linked to the formation of a catalytic triad and cofactor binding, and crystal structures of FabG from different organisms have been captured in either active or inactive conformations. However, the structural elements which enable activation of FabG require further exploration. Here we report structural, enzymatic, and binding studies of vcFabG, the FabG protein found in the causative agent of cholera, Vibrio cholerae. vcFabG exists predominantly as a dimer in solution and is able to self-associate to form tetramers, which is the state seen in the crystal structure. The formation of the tetramer may be promoted by the presence of the cofactor NADP(H). The transition between dimeric and tetrameric states of vcFabG is related to changes in the conformations of the alpha4/alpha5 helices on the dimer-dimer interface. Two glycine residues adjacent to the dimer interface (G92 and G141) are identified as the hinge for the conformational changes, while the catalytic tyrosine (Y155) and a glutamine residue that forms hydrogen bonds to both loops beta4-alpha4 and beta5-alpha5 (Q152) stabilize the active conformation. The functions of the aforementioned residues were confirmed by the binding and enzymatic assays for the corresponding mutants. IMPORTANCE: This paper describes structural, enzymatic, and binding studies of FabG from Vibrio cholera. In this work we dissect the structural elements responsible for the activation of vcFabG. The structural information provided here is essential for the development of antibiotics specifically targeting bacteria FabG, especially for the multi-drug resistant strains of V. cholerae.
-
-	Dissecting the structural elements for the activation of beta-ketoacyl-(acyl carrier protein) reductase from Vibrio cholerae.,Hou J, Zheng H, Chruszcz M, Zimmerman MD, Shumilin IA, Osinski T, Demas M, Grimshaw S, Minor W J Bacteriol. 2015 Nov 9. pii: JB.00360-15. PMID:26553852<ref>PMID:26553852</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 3rro" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Beta-ketoacyl carrier protein reductase 3D structures\|Beta-ketoacyl carrier protein reductase 3D structures]]		*[[Beta-ketoacyl carrier protein reductase 3D structures\|Beta-ketoacyl carrier protein reductase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 12:46, 17 January 2024

OCA — Wed, 17 Jan 2024 12:46:57 GMT

←Older revision		Revision as of 12:46, 17 January 2024
Line 3:		Line 3:
	<StructureSection load='3rro' size='340' side='right'caption='[[3rro]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3rro' size='340' side='right'caption='[[3rro]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rro]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Vibch Vibch~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RRO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RRO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rro]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae_O1_biovar_El_Tor_str._N16961 Vibrio cholerae O1 biovar El Tor str. N16961]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RRO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RRO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3op4\|3op4]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VCD_002346 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243277 VIBCH])</td></tr>	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rro OCA], [https://pdbe.org/3rro PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rro RCSB], [https://www.ebi.ac.uk/pdbsum/3rro PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rro ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rro OCA], [https://pdbe.org/3rro PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rro RCSB], [https://www.ebi.ac.uk/pdbsum/3rro PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rro ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/FABG_VIBCH FABG_VIBCH] Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Vibch~~]]	+	[[Category: Vibrio cholerae O1 biovar El Tor str. N16961]]
-	[[Category: Anderson, W]]	+	[[Category: Anderson W]]
-	~~[[Category: Structural genomic~~]]	+	[[Category: Chruszcz M]]
-	[[Category: Chruszcz, M]]	+	[[Category: Cooper DR]]
-	[[Category: Cooper~~, D R~~]]	+	[[Category: Grabowski M]]
-	[[Category: Grabowski, M]]	+	[[Category: Hou J]]
-	[[Category: Hou, J]]	+	[[Category: Minor W]]
-	[[Category: Minor, W]]	+	[[Category: Osinski T]]
-	[[Category: Osinski, T]]	+	[[Category: Shumilin I]]
-	[[Category: Shumilin, I]]	+	[[Category: Zheng H]]
-	[[Category: Zheng, H]]	+
-	~~[[Category: Acyl carrier protein]]~~	+
-	~~[[Category: Cell plasma]]~~	+
-	~~[[Category: Csgid]]~~	+
-	~~[[Category: Fabg]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 09:58, 13 April 2022

OCA — Wed, 13 Apr 2022 09:58:26 GMT

←Older revision		Revision as of 09:58, 13 April 2022
Line 1:		Line 1:

	==Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (FabG) from Vibrio cholerae==		==Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (FabG) from Vibrio cholerae==
-	<StructureSection load='3rro' size='340' side='right' caption='[[3rro]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='3rro' size='340' side='right'caption='[[3rro]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rro]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Vibch Vibch]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RRO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3RRO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rro]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibch Vibch]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RRO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RRO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3op4\|3op4]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3op4\|3op4]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VCD_002346 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243277 VIBCH])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VCD_002346 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243277 VIBCH])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3rro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rro OCA], [~~http~~://pdbe.org/3rro PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3rro RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3rro PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rro OCA], [https://pdbe.org/3rro PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rro RCSB], [https://www.ebi.ac.uk/pdbsum/3rro PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rro ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 21:

	==See Also==		==See Also==
-	*[[Beta-ketoacyl~~-ACP~~ reductase\|Beta-ketoacyl~~-ACP~~ reductase]]	+	*[[Beta-ketoacyl carrier protein reductase 3D structures\|Beta-ketoacyl carrier protein reductase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Vibch]]		[[Category: Vibch]]
	[[Category: Anderson, W]]		[[Category: Anderson, W]]

OCA at 16:41, 10 May 2016

OCA — Tue, 10 May 2016 16:41:58 GMT

←Older revision		Revision as of 16:41, 10 May 2016
Line 1:		Line 1:
		+
	==Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (FabG) from Vibrio cholerae==		==Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (FabG) from Vibrio cholerae==
	<StructureSection load='3rro' size='340' side='right' caption='[[3rro]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3rro' size='340' side='right' caption='[[3rro]], [[Resolution\|resolution]] 2.00Å' scene=''>

OCA at 20:15, 30 November 2015

OCA — Mon, 30 Nov 2015 20:15:22 GMT

←Older revision		Revision as of 20:15, 30 November 2015
Line 2:		Line 2:
	<StructureSection load='3rro' size='340' side='right' caption='[[3rro]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3rro' size='340' side='right' caption='[[3rro]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rro]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Vibrio_cholerae_o1_biovar_el_tor Vibrio cholerae o1 biovar el tor~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RRO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RRO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rro]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibch Vibch]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RRO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RRO FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3op4\|3op4]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3op4\|3op4]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VCD_002346 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~686 Vibrio cholerae O1 biovar El Tor~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VCD_002346 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243277 VIBCH])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rro OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rro RCSB], [http://www.ebi.ac.uk/pdbsum/3rro PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rro OCA], [http://pdbe.org/3rro PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3rro RCSB], [http://www.ebi.ac.uk/pdbsum/3rro PDBsum]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	beta-ketoacyl-(acyl carrier protein) reductase (FabG) catalyzes the key reductive reaction in the elongation cycle of fatty acid synthesis (FAS), which is a vital metabolic pathway in bacteria and a promising target for new antibiotic development. The activation of the enzyme is usually linked to the formation of a catalytic triad and cofactor binding, and crystal structures of FabG from different organisms have been captured in either active or inactive conformations. However, the structural elements which enable activation of FabG require further exploration. Here we report structural, enzymatic, and binding studies of vcFabG, the FabG protein found in the causative agent of cholera, Vibrio cholerae. vcFabG exists predominantly as a dimer in solution and is able to self-associate to form tetramers, which is the state seen in the crystal structure. The formation of the tetramer may be promoted by the presence of the cofactor NADP(H). The transition between dimeric and tetrameric states of vcFabG is related to changes in the conformations of the alpha4/alpha5 helices on the dimer-dimer interface. Two glycine residues adjacent to the dimer interface (G92 and G141) are identified as the hinge for the conformational changes, while the catalytic tyrosine (Y155) and a glutamine residue that forms hydrogen bonds to both loops beta4-alpha4 and beta5-alpha5 (Q152) stabilize the active conformation. The functions of the aforementioned residues were confirmed by the binding and enzymatic assays for the corresponding mutants. IMPORTANCE: This paper describes structural, enzymatic, and binding studies of FabG from Vibrio cholera. In this work we dissect the structural elements responsible for the activation of vcFabG. The structural information provided here is essential for the development of antibiotics specifically targeting bacteria FabG, especially for the multi-drug resistant strains of V. cholerae.
		+
		+	Dissecting the structural elements for the activation of beta-ketoacyl-(acyl carrier protein) reductase from Vibrio cholerae.,Hou J, Zheng H, Chruszcz M, Zimmerman MD, Shumilin IA, Osinski T, Demas M, Grimshaw S, Minor W J Bacteriol. 2015 Nov 9. pii: JB.00360-15. PMID:26553852<ref>PMID:26553852</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 3rro" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Beta-ketoacyl-ACP reductase\|Beta-ketoacyl-ACP reductase]]		*[[Beta-ketoacyl-ACP reductase\|Beta-ketoacyl-ACP reductase]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Vibrio cholerae o1 biovar el tor~~]]	+	[[Category: Vibch]]
	[[Category: Anderson, W]]		[[Category: Anderson, W]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]

OCA at 09:39, 4 January 2015

OCA — Sun, 04 Jan 2015 09:39:58 GMT

←Older revision		Revision as of 09:39, 4 January 2015
Line 1:		Line 1:
-	~~{{STRUCTURE_3rro\| PDB=3rro \| SCENE= }}~~	+	==Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (FabG) from Vibrio cholerae==
-	===Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (FabG) from Vibrio cholerae===	+	<StructureSection load='3rro' size='340' side='right' caption='[[3rro]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3rro]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae_o1_biovar_el_tor Vibrio cholerae o1 biovar el tor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RRO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RRO FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3op4\|3op4]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VCD_002346 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=686 Vibrio cholerae O1 biovar El Tor])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rro OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rro RCSB], [http://www.ebi.ac.uk/pdbsum/3rro PDBsum]</span></td></tr>
		+	</table>

-	==~~About this Structure~~==	+	==See Also==
-	[[~~3rro~~]] ~~is a 2 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Vibrio_cholerae_o1_biovar_el_tor Vibrio cholerae o1 biovar el tor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RRO OCA].~~	+	*[[Beta-ketoacyl-ACP reductase\|Beta-ketoacyl-ACP reductase]]
		+	__TOC__
		+	</StructureSection>
	[[Category: Vibrio cholerae o1 biovar el tor]]		[[Category: Vibrio cholerae o1 biovar el tor]]
-	[[Category: Anderson, W.]]	+	[[Category: Anderson, W]]
-	[[Category: ~~CSGID, Center for~~ Structural ~~Genomics of Infectious Diseases.~~]]	+	[[Category: Structural genomic]]
-	[[Category: Chruszcz, M.]]	+	[[Category: Chruszcz, M]]
-	[[Category: Cooper, D R.]]	+	[[Category: Cooper, D R]]
-	[[Category: Grabowski, M.]]	+	[[Category: Grabowski, M]]
-	[[Category: Hou, J.]]	+	[[Category: Hou, J]]
-	[[Category: Minor, W.]]	+	[[Category: Minor, W]]
-	[[Category: Osinski, T.]]	+	[[Category: Osinski, T]]
-	[[Category: Shumilin, I.]]	+	[[Category: Shumilin, I]]
-	[[Category: Zheng, H.]]	+	[[Category: Zheng, H]]
	[[Category: Acyl carrier protein]]		[[Category: Acyl carrier protein]]
	[[Category: Cell plasma]]		[[Category: Cell plasma]]
-	[[Category: Center for structural genomics of infectious disease]]
	[[Category: Csgid]]		[[Category: Csgid]]
	[[Category: Fabg]]		[[Category: Fabg]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
-	[[Category: Structural genomic]]

OCA at 08:11, 16 May 2013

OCA — Thu, 16 May 2013 08:11:07 GMT

←Older revision		Revision as of 08:11, 16 May 2013
Line 1:		Line 1:
-	[[Image:3rro.jpg\|left\|200px]]
-
-	<!--
-	The line below this paragraph, containing "STRUCTURE_3rro", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3rro\| PDB=3rro \| SCENE= }}		{{STRUCTURE_3rro\| PDB=3rro \| SCENE= }}
-
	===Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (FabG) from Vibrio cholerae===		===Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (FabG) from Vibrio cholerae===
-

	==About this Structure==		==About this Structure==
Line 25:		Line 15:
	[[Category: Shumilin, I.]]		[[Category: Shumilin, I.]]
	[[Category: Zheng, H.]]		[[Category: Zheng, H.]]
		+	[[Category: Acyl carrier protein]]
		+	[[Category: Cell plasma]]
		+	[[Category: Center for structural genomics of infectious disease]]
		+	[[Category: Csgid]]
		+	[[Category: Fabg]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Structural genomic]]

OCA at 05:57, 18 May 2011

OCA — Wed, 18 May 2011 05:57:38 GMT

←Older revision		Revision as of 05:57, 18 May 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3rro.jpg\|left\|200px]]

-	The ~~entry 3rro~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3rro", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3rro\| PDB=3rro \| SCENE= }}

-	~~Authors: Hou, J., Chruszcz, M., Cooper, D.R., Grabowski, M., Zheng, H., Osinski, T., Shumilin, I., Anderson, W., Minor, W., Center for Structural Genomics~~ of ~~Infectious Diseases~~ (~~CSGID~~)	+	===Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (FabG) from Vibrio cholerae===

-	~~Description: Crystal~~ structure ~~of 3-ketoacyl-(acyl-carrier-protein) reductase (FabG)~~ from Vibrio cholerae	+
		+	==About this Structure==
		+	[[3rro]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae_o1_biovar_el_tor Vibrio cholerae o1 biovar el tor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RRO OCA].
		+	[[Category: Vibrio cholerae o1 biovar el tor]]
		+	[[Category: Anderson, W.]]
		+	[[Category: CSGID, Center for Structural Genomics of Infectious Diseases.]]
		+	[[Category: Chruszcz, M.]]
		+	[[Category: Cooper, D R.]]
		+	[[Category: Grabowski, M.]]
		+	[[Category: Hou, J.]]
		+	[[Category: Minor, W.]]
		+	[[Category: Osinski, T.]]
		+	[[Category: Shumilin, I.]]
		+	[[Category: Zheng, H.]]

OCA: Protected "3rro" [edit=sysop:move=sysop]

OCA — Thu, 12 May 2011 04:22:32 GMT

Protected "3rro" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:22, 12 May 2011

OCA: New page: '''Unreleased structure''' The entry 3rro is ON HOLD Authors: Hou, J., Chruszcz, M., Cooper, D.R., Grabowski, M., Zheng, H., Osinski, T., Shumilin, I., Anderson, W., Minor, W., Center f...

OCA — Thu, 12 May 2011 04:22:31 GMT

New page: '''Unreleased structure''' The entry 3rro is ON HOLD Authors: Hou, J., Chruszcz, M., Cooper, D.R., Grabowski, M., Zheng, H., Osinski, T., Shumilin, I., Anderson, W., Minor, W., Center f...

New page

'''Unreleased structure'''

The entry 3rro is ON HOLD

Authors: Hou, J., Chruszcz, M., Cooper, D.R., Grabowski, M., Zheng, H., Osinski, T., Shumilin, I., Anderson, W., Minor, W., Center for Structural Genomics of Infectious Diseases (CSGID)

Description: Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (FabG) from Vibrio cholerae