3rw9 - Revision history

OCA at 09:45, 1 March 2024

OCA — Fri, 01 Mar 2024 09:45:48 GMT

←Older revision		Revision as of 09:45, 1 March 2024
Line 3:		Line 3:
	<StructureSection load='3rw9' size='340' side='right'caption='[[3rw9]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3rw9' size='340' side='right'caption='[[3rw9]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rw9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RW9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RW9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rw9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RW9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RW9 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=DSH:5~~-~~S-(3-AMINOPROPYL)-5-THIOADENOSINE'>DSH</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>SRM, SPS1, SRML1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id~~=9606 HUMAN])</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DSH:5-S-(3-AMINOPROPYL)-5-THIOADENOSINE'>DSH</scene></td></tr>
-	~~<tr id='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[https~~:~~//en.wikipedia.org/wiki/Spermidine_synthase Spermidine synthase], with EC number [https://www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=2.~~5~~.1.16 2.5.1.16]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rw9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rw9 OCA], [https://pdbe.org/3rw9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rw9 RCSB], [https://www.ebi.ac.uk/pdbsum/3rw9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rw9 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rw9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rw9 OCA], [https://pdbe.org/3rw9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rw9 RCSB], [https://www.ebi.ac.uk/pdbsum/3rw9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rw9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/SPEE_HUMAN SPEE_HUMAN]] Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM). Has a strong preference for putrescine as substrate, and has very low activity towards 1,3-diaminopropane. Has extremely low activity towards spermidine.	+	[https://www.uniprot.org/uniprot/SPEE_HUMAN SPEE_HUMAN] Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM). Has a strong preference for putrescine as substrate, and has very low activity towards 1,3-diaminopropane. Has extremely low activity towards spermidine.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Aminopropyltransferases are essential enzymes that form polyamines in eukaryotic and most prokaryotic cells. Spermidine synthase (SpdS) is one of the most well-studied enzymes in this biosynthetic pathway. The enzyme uses decarboxylated S-adenosylmethionine and a short-chain polyamine (putrescine) to make a medium-chain polyamine (spermidine) and 5'-deoxy-5'-methylthioadenosine as a byproduct. Here, we report a new spermidine synthase inhibitor, decarboxylated S-adenosylhomocysteine (dcSAH). The inhibitor was synthesized, and dose-dependent inhibition of human, Thermatoga maritima, and Plasmodium falciparum spermidine synthases, as well as functionally homologous human spermine synthase, was determined. The human SpdS/dcSAH complex structure was determined by X-ray crystallography at 2.0 A resolution and showed consistent active site positioning and coordination with previously known structures. Isothermal calorimetry binding assays confirmed inhibitor binding to human SpdS with K(d) of 1.1 +/- 0.3 muM in the absence of putrescine and 3.2 +/- 0.1 muM in the presence of putrescine. These results indicate a potential for further inhibitor development based on the dcSAH scaffold.	+
-		+
-	Binding and inhibition of human spermidine synthase by decarboxylated S-adenosylhomocysteine.,Seckute J, McCloskey DE, Thomas HJ, Secrist JA 3rd, Pegg AE, Ealick SE Protein Sci. 2011 Aug 24. doi: 10.1002/pro.717. PMID:21898642<ref>PMID:21898642</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3rw9" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Spermidine synthase 3D structures\|Spermidine synthase 3D structures]]		*[[Spermidine synthase 3D structures\|Spermidine synthase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Spermidine synthase]]~~	+	[[Category: Ealick SE]]
-	[[Category: Ealick~~, S E]]~~	+	[[Category: McCloskey DE]]
-	~~[[Category: III, J A.Secrist~~]]	+	[[Category: Pegg AE]]
-	[[Category: McCloskey~~, D E~~]]	+	[[Category: Seckute J]]
-	[[Category: Pegg~~, A E~~]]	+	[[Category: Secrist III JA]]
-	[[Category: Seckute, J]]	+	[[Category: Thomas HJ]]
-	[[Category: ~~Thomas, H J~~]]	+
-	[[Category: ~~Aminopropyltransferase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 10:27, 22 June 2022

OCA — Wed, 22 Jun 2022 10:27:43 GMT

←Older revision		Revision as of 10:27, 22 June 2022
Line 1:		Line 1:

	==Crystal Structure of human Spermidine Synthase in Complex with decarboxylated S-adenosylhomocysteine==		==Crystal Structure of human Spermidine Synthase in Complex with decarboxylated S-adenosylhomocysteine==
-	<StructureSection load='3rw9' size='340' side='right' caption='[[3rw9]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='3rw9' size='340' side='right'caption='[[3rw9]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rw9]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RW9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3RW9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rw9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RW9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RW9 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DSH:5-S-(3-AMINOPROPYL)-5-THIOADENOSINE'>DSH</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DSH:5-S-(3-AMINOPROPYL)-5-THIOADENOSINE'>DSH</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SRM, SPS1, SRML1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SRM, SPS1, SRML1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Spermidine_synthase Spermidine synthase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.16 2.5.1.16] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Spermidine_synthase Spermidine synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.16 2.5.1.16] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3rw9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rw9 OCA], [~~http~~://pdbe.org/3rw9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3rw9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3rw9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3rw9 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rw9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rw9 OCA], [https://pdbe.org/3rw9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rw9 RCSB], [https://www.ebi.ac.uk/pdbsum/3rw9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rw9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/SPEE_HUMAN SPEE_HUMAN]] Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM). Has a strong preference for putrescine as substrate, and has very low activity towards 1,3-diaminopropane. Has extremely low activity towards spermidine.	+	[[https://www.uniprot.org/uniprot/SPEE_HUMAN SPEE_HUMAN]] Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM). Has a strong preference for putrescine as substrate, and has very low activity towards 1,3-diaminopropane. Has extremely low activity towards spermidine.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:

	==See Also==		==See Also==
-	*[[Spermidine ~~Synthase~~\|Spermidine ~~Synthase~~]]	+	*[[Spermidine synthase 3D structures\|Spermidine synthase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 28:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Spermidine synthase]]		[[Category: Spermidine synthase]]
	[[Category: Ealick, S E]]		[[Category: Ealick, S E]]

OCA at 02:46, 5 August 2016

OCA — Fri, 05 Aug 2016 02:46:36 GMT

←Older revision		Revision as of 02:46, 5 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of human Spermidine Synthase in Complex with decarboxylated S-adenosylhomocysteine==		==Crystal Structure of human Spermidine Synthase in Complex with decarboxylated S-adenosylhomocysteine==
	<StructureSection load='3rw9' size='340' side='right' caption='[[3rw9]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3rw9' size='340' side='right' caption='[[3rw9]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3rw9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RW9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RW9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3rw9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RW9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RW9 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DSH:5-S-(3-AMINOPROPYL)-5-THIOADENOSINE'>DSH</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DSH:5-S-(3-AMINOPROPYL)-5-THIOADENOSINE'>DSH</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SRM, SPS1, SRML1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SRM, SPS1, SRML1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Spermidine_synthase Spermidine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.16 2.5.1.16] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Spermidine_synthase Spermidine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.16 2.5.1.16] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rw9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rw9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rw9 RCSB], [http://www.ebi.ac.uk/pdbsum/3rw9 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rw9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rw9 OCA], [http://pdbe.org/3rw9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3rw9 RCSB], [http://www.ebi.ac.uk/pdbsum/3rw9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3rw9 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3rw9" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 25:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Spermidine synthase]]		[[Category: Spermidine synthase]]
	[[Category: Ealick, S E]]		[[Category: Ealick, S E]]

OCA at 22:09, 24 December 2014

OCA — Wed, 24 Dec 2014 22:09:42 GMT

←Older revision		Revision as of 22:09, 24 December 2014
Line 8:		Line 8:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rw9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rw9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rw9 RCSB], [http://www.ebi.ac.uk/pdbsum/3rw9 PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rw9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rw9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rw9 RCSB], [http://www.ebi.ac.uk/pdbsum/3rw9 PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SPEE_HUMAN SPEE_HUMAN]] Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM). Has a strong preference for putrescine as substrate, and has very low activity towards 1,3-diaminopropane. Has extremely low activity towards spermidine.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 10:55, 9 December 2014

OCA — Tue, 09 Dec 2014 10:55:48 GMT

←Older revision		Revision as of 10:55, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3rw9.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal Structure of human Spermidine Synthase in Complex with decarboxylated S-adenosylhomocysteine==
		+	<StructureSection load='3rw9' size='340' side='right' caption='[[3rw9]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3rw9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RW9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RW9 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DSH:5-S-(3-AMINOPROPYL)-5-THIOADENOSINE'>DSH</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SRM, SPS1, SRML1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Spermidine_synthase Spermidine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.16 2.5.1.16] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rw9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rw9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rw9 RCSB], [http://www.ebi.ac.uk/pdbsum/3rw9 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Aminopropyltransferases are essential enzymes that form polyamines in eukaryotic and most prokaryotic cells. Spermidine synthase (SpdS) is one of the most well-studied enzymes in this biosynthetic pathway. The enzyme uses decarboxylated S-adenosylmethionine and a short-chain polyamine (putrescine) to make a medium-chain polyamine (spermidine) and 5'-deoxy-5'-methylthioadenosine as a byproduct. Here, we report a new spermidine synthase inhibitor, decarboxylated S-adenosylhomocysteine (dcSAH). The inhibitor was synthesized, and dose-dependent inhibition of human, Thermatoga maritima, and Plasmodium falciparum spermidine synthases, as well as functionally homologous human spermine synthase, was determined. The human SpdS/dcSAH complex structure was determined by X-ray crystallography at 2.0 A resolution and showed consistent active site positioning and coordination with previously known structures. Isothermal calorimetry binding assays confirmed inhibitor binding to human SpdS with K(d) of 1.1 +/- 0.3 muM in the absence of putrescine and 3.2 +/- 0.1 muM in the presence of putrescine. These results indicate a potential for further inhibitor development based on the dcSAH scaffold.

-	~~{{STRUCTURE_3rw9\| PDB=3rw9 \| SCENE= }}~~	+	Binding and inhibition of human spermidine synthase by decarboxylated S-adenosylhomocysteine.,Seckute J, McCloskey DE, Thomas HJ, Secrist JA 3rd, Pegg AE, Ealick SE Protein Sci. 2011 Aug 24. doi: 10.1002/pro.717. PMID:21898642<ref>PMID:21898642</ref>

-	~~===Crystal Structure~~ of ~~human Spermidine Synthase in Complex with decarboxylated~~ S~~-adenosylhomocysteine===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_21898642}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3rw9]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org~~/~~wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RW9 OCA].~~	+

	==See Also==		==See Also==
	*[[Spermidine Synthase\|Spermidine Synthase]]		*[[Spermidine Synthase\|Spermidine Synthase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:021898642<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Spermidine synthase]]		[[Category: Spermidine synthase]]
-	[[Category: Ealick, S E.]]	+	[[Category: Ealick, S E]]
-	[[Category: III, J A.Secrist.]]	+	[[Category: III, J A.Secrist]]
-	[[Category: McCloskey, D E.]]	+	[[Category: McCloskey, D E]]
-	[[Category: Pegg, A E.]]	+	[[Category: Pegg, A E]]
-	[[Category: Seckute, J.]]	+	[[Category: Seckute, J]]
-	[[Category: Thomas, H J.]]	+	[[Category: Thomas, H J]]
	[[Category: Aminopropyltransferase]]		[[Category: Aminopropyltransferase]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 09:38, 20 October 2012

OCA — Sat, 20 Oct 2012 09:38:38 GMT

←Older revision		Revision as of 09:38, 20 October 2012
Line 1:		Line 1:
	[[Image:3rw9.png\|left\|200px]]		[[Image:3rw9.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3rw9", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3rw9\| PDB=3rw9 \| SCENE= }}		{{STRUCTURE_3rw9\| PDB=3rw9 \| SCENE= }}

	===Crystal Structure of human Spermidine Synthase in Complex with decarboxylated S-adenosylhomocysteine===		===Crystal Structure of human Spermidine Synthase in Complex with decarboxylated S-adenosylhomocysteine===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21898642}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 21898642 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_21898642}}		{{ABSTRACT_PUBMED_21898642}}

	==About this Structure==		==About this Structure==
	[[3rw9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RW9 OCA].		[[3rw9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RW9 OCA].
		+
		+	==See Also==
		+	*[[Spermidine Synthase\|Spermidine Synthase]]

	==Reference==		==Reference==

OCA at 07:39, 2 November 2011

OCA — Wed, 02 Nov 2011 07:39:59 GMT

←Older revision		Revision as of 07:39, 2 November 2011
Line 1:		Line 1:
-	[[Image:3rw9.~~jpg~~\|left\|200px]]	+	[[Image:3rw9.png\|left\|200px]]

	<!--		<!--

OCA at 06:00, 21 September 2011

OCA — Wed, 21 Sep 2011 06:00:49 GMT

←Older revision		Revision as of 06:00, 21 September 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3rw9.jpg\|left\|200px]]

-	The ~~entry 3rw9~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3rw9", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3rw9\| PDB=3rw9 \| SCENE= }}

-	~~Authors: Seckute, J., McCloskey, D.E., Thomas, H.J., Secrist III, J.A., Pegg, A.E., Ealick,~~ S~~.E.~~	+	===Crystal Structure of human Spermidine Synthase in Complex with decarboxylated S-adenosylhomocysteine===

-	~~Description~~: ~~Crystal~~ Structure ~~of human Spermidine Synthase in Complex~~ with ~~decarboxylated S~~-~~adenosylhomocysteine~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21898642}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21898642 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21898642}}
		+
		+	==About this Structure==
		+	[[3rw9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RW9 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021898642</ref><references group="xtra"/>
		+	[[Category: Homo sapiens]]
		+	[[Category: Spermidine synthase]]
		+	[[Category: Ealick, S E.]]
		+	[[Category: III, J A.Secrist.]]
		+	[[Category: McCloskey, D E.]]
		+	[[Category: Pegg, A E.]]
		+	[[Category: Seckute, J.]]
		+	[[Category: Thomas, H J.]]
		+	[[Category: Aminopropyltransferase]]
		+	[[Category: Transferase]]

OCA at 05:52, 15 June 2011

OCA — Wed, 15 Jun 2011 05:52:24 GMT

←Older revision		Revision as of 05:52, 15 June 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3rw9 is ON HOLD	+	The entry 3rw9 is ON HOLD until Paper Publication

	Authors: Seckute, J., McCloskey, D.E., Thomas, H.J., Secrist III, J.A., Pegg, A.E., Ealick, S.E.		Authors: Seckute, J., McCloskey, D.E., Thomas, H.J., Secrist III, J.A., Pegg, A.E., Ealick, S.E.

	Description: Crystal Structure of human Spermidine Synthase in Complex with decarboxylated S-adenosylhomocysteine		Description: Crystal Structure of human Spermidine Synthase in Complex with decarboxylated S-adenosylhomocysteine

OCA: Protected "3rw9" [edit=sysop:move=sysop]

OCA — Wed, 18 May 2011 05:17:08 GMT

Protected "3rw9" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:17, 18 May 2011