3s1f - Revision history

OCA at 12:43, 14 March 2024

OCA — Thu, 14 Mar 2024 12:43:03 GMT

←Older revision		Revision as of 12:43, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3s1f' size='340' side='right'caption='[[3s1f]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3s1f' size='340' side='right'caption='[[3s1f]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3s1f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Maize Maize~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S1F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3S1F FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3s1f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S1F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3S1F FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=ZIP:N-(3-METHYLBUT-2-EN-1-YL)-9H-PURIN-6-AMINE'>ZIP</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3bw7\|3bw7]], [[3c0p\|3c0p]], [[2qkn\|2qkn]], [[1w1q\|1w1q]], [[1w1r\|1w1r]], [[1w1s\|1w1s]], [[3dq0\|3dq0]], [[2qpm\|2qpm]], [[3kjm\|3kjm]], [[3s1c\|3s1c]], [[3s1d\|3s1d]], [[3s1e\|3s1e]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=ZIP:N-(3-METHYLBUT-2-EN-1-YL)-9H-PURIN-6-AMINE'>ZIP</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CKX1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4577 MAIZE])</td></tr>~~	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Cytokinin_dehydrogenase Cytokinin dehydrogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.99.12 1.5.99.12] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3s1f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s1f OCA], [https://pdbe.org/3s1f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3s1f RCSB], [https://www.ebi.ac.uk/pdbsum/3s1f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3s1f ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3s1f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s1f OCA], [https://pdbe.org/3s1f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3s1f RCSB], [https://www.ebi.ac.uk/pdbsum/3s1f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3s1f ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CKX1_MAIZE CKX1_MAIZE]] Catalyzes the oxidation of cytokinins, a family of N(6)-substituted adenine derivatives that are plant hormones, where the substituent is an isopentenyl group. Cleaves zeatin, isopentenyladenine, isopentenyladenosine, zeatin riboside and cis-zeatin, but not dihydrozeatin, kinetin and benzylaminopurine.	+	[https://www.uniprot.org/uniprot/CKX1_MAIZE CKX1_MAIZE] Catalyzes the oxidation of cytokinins, a family of N(6)-substituted adenine derivatives that are plant hormones, where the substituent is an isopentenyl group. Cleaves zeatin, isopentenyladenine, isopentenyladenosine, zeatin riboside and cis-zeatin, but not dihydrozeatin, kinetin and benzylaminopurine.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Cytokinins are hormones that regulate plant development and their environmental responses. Their levels are mainly controlled by the cytokinin oxidase/dehydrogenase (CKO), which oxidatively cleaves cytokinins using redox-active electron acceptors. CKO belongs to the group of flavoproteins with an 8alpha-N1-histidyl FAD covalent linkage. Here, we investigated the role of seven active site residues: H105, D169, E288, V378, E381, P427 and L492, in substrate binding and catalysis of the CKO1 from maize (Zea mays, ZmCKO1) combining site-directed mutagenesis with kinetics and X-ray crystallography. Here, we identify E381 as a key residue for enzyme specificity that restricts substrate binding as well as quinone electron acceptor binding. We show that D169 is important for catalysis and that H105 covalently linked to FAD maintains the enzyme's structural integrity, stability and high rates with electron acceptors. The L492A mutation significantly modulates the cleavage of aromatic cytokinins and zeatin isomers. The high resolution X-ray structures of ZmCKO1 and the E381S variant in complex with N6-(2-isopentenyl)adenosine (iPR) reveal the binding mode of cytokinin ribosides. Those of ZmCKO2 and ZmCKO4a contain a mobile domain, which might contribute to binding of the N9 substituted cytokinins. This article is protected by copyright. All rights reserved.	+
-		+
-	Kinetic and structural investigation of the cytokinin oxidase/dehydrogenase active site.,Kopecny D, Koncitikova R, Popelka H, Briozzo P, Vigouroux A, Kopecna M, Zalabak D, Sebela M, Skopalova J, Frebort I, Morera S FEBS J. 2015 Oct 31. doi: 10.1111/febs.13581. PMID:26519657<ref>PMID:26519657</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3s1f" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Cytokinin dehydrogenase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Maize~~]]	+	[[Category: Zea mays]]
-	[[Category: Briozzo, P]]	+	[[Category: Briozzo P]]
-	[[Category: Kopecny, D]]	+	[[Category: Kopecny D]]
-	[[Category: Morera, S]]	+	[[Category: Morera S]]
-	~~[[Category: Covalent flavination]]~~	+
-	~~[[Category: Cytokinin binding]]~~	+
-	~~[[Category: Cytokinin oxidase/dehydrogenase]]~~	+
-	~~[[Category: Fad binding protein]]~~	+
-	~~[[Category: Flavoprotein]]~~	+
-	~~[[Category: Glycosylation]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 10:33, 22 June 2022

OCA — Wed, 22 Jun 2022 10:33:53 GMT

←Older revision		Revision as of 10:33, 22 June 2022
Line 1:		Line 1:

	==Asp169Glu mutant of maize cytokinin oxidase/dehydrogenase complexed with N6-isopentenyladenine==		==Asp169Glu mutant of maize cytokinin oxidase/dehydrogenase complexed with N6-isopentenyladenine==
-	<StructureSection load='3s1f' size='340' side='right' caption='[[3s1f]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='3s1f' size='340' side='right'caption='[[3s1f]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3s1f]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Maize Maize]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S1F OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3S1F FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3s1f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Maize Maize]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S1F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3S1F FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=ZIP:N-(3-METHYLBUT-2-EN-1-YL)-9H-PURIN-6-AMINE'>ZIP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=ZIP:N-(3-METHYLBUT-2-EN-1-YL)-9H-PURIN-6-AMINE'>ZIP</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bw7\|3bw7]], [[3c0p\|3c0p]], [[2qkn\|2qkn]], [[1w1q\|1w1q]], [[1w1r\|1w1r]], [[1w1s\|1w1s]], [[3dq0\|3dq0]], [[2qpm\|2qpm]], [[3kjm\|3kjm]], [[3s1c\|3s1c]], [[3s1d\|3s1d]], [[3s1e\|3s1e]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3bw7\|3bw7]], [[3c0p\|3c0p]], [[2qkn\|2qkn]], [[1w1q\|1w1q]], [[1w1r\|1w1r]], [[1w1s\|1w1s]], [[3dq0\|3dq0]], [[2qpm\|2qpm]], [[3kjm\|3kjm]], [[3s1c\|3s1c]], [[3s1d\|3s1d]], [[3s1e\|3s1e]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CKX1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4577 MAIZE])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CKX1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4577 MAIZE])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Cytokinin_dehydrogenase Cytokinin dehydrogenase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.99.12 1.5.99.12] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Cytokinin_dehydrogenase Cytokinin dehydrogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.99.12 1.5.99.12] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3s1f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s1f OCA], [~~http~~://pdbe.org/3s1f PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3s1f RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3s1f PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3s1f ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3s1f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s1f OCA], [https://pdbe.org/3s1f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3s1f RCSB], [https://www.ebi.ac.uk/pdbsum/3s1f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3s1f ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CKX1_MAIZE CKX1_MAIZE]] Catalyzes the oxidation of cytokinins, a family of N(6)-substituted adenine derivatives that are plant hormones, where the substituent is an isopentenyl group. Cleaves zeatin, isopentenyladenine, isopentenyladenosine, zeatin riboside and cis-zeatin, but not dihydrozeatin, kinetin and benzylaminopurine.	+	[[https://www.uniprot.org/uniprot/CKX1_MAIZE CKX1_MAIZE]] Catalyzes the oxidation of cytokinins, a family of N(6)-substituted adenine derivatives that are plant hormones, where the substituent is an isopentenyl group. Cleaves zeatin, isopentenyladenine, isopentenyladenosine, zeatin riboside and cis-zeatin, but not dihydrozeatin, kinetin and benzylaminopurine.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Cytokinin dehydrogenase]]		[[Category: Cytokinin dehydrogenase]]
		+	[[Category: Large Structures]]
	[[Category: Maize]]		[[Category: Maize]]
	[[Category: Briozzo, P]]		[[Category: Briozzo, P]]

OCA at 12:53, 16 November 2017

OCA — Thu, 16 Nov 2017 12:53:42 GMT

←Older revision		Revision as of 12:53, 16 November 2017
Line 8:		Line 8:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CKX1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4577 MAIZE])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CKX1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4577 MAIZE])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cytokinin_dehydrogenase Cytokinin dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.99.12 1.5.99.12] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cytokinin_dehydrogenase Cytokinin dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.99.12 1.5.99.12] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s1f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s1f OCA], [http://pdbe.org/3s1f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3s1f RCSB], [http://www.ebi.ac.uk/pdbsum/3s1f PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s1f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s1f OCA], [http://pdbe.org/3s1f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3s1f RCSB], [http://www.ebi.ac.uk/pdbsum/3s1f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3s1f ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 17:05, 10 May 2016

OCA — Tue, 10 May 2016 17:05:57 GMT

←Older revision		Revision as of 17:05, 10 May 2016
Line 1:		Line 1:
		+
	==Asp169Glu mutant of maize cytokinin oxidase/dehydrogenase complexed with N6-isopentenyladenine==		==Asp169Glu mutant of maize cytokinin oxidase/dehydrogenase complexed with N6-isopentenyladenine==
	<StructureSection load='3s1f' size='340' side='right' caption='[[3s1f]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3s1f' size='340' side='right' caption='[[3s1f]], [[Resolution\|resolution]] 2.00Å' scene=''>

OCA at 06:25, 23 December 2015

OCA — Wed, 23 Dec 2015 06:25:57 GMT

←Older revision		Revision as of 06:25, 23 December 2015
Line 11:		Line 11:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/CKX1_MAIZE CKX1_MAIZE]] Catalyzes the oxidation of cytokinins, a family of N(6)-substituted adenine derivatives that are plant hormones, where the substituent is an isopentenyl group. Cleaves zeatin, isopentenyladenine, isopentenyladenosine, zeatin riboside and cis-zeatin, but not dihydrozeatin, kinetin and benzylaminopurine.		[[http://www.uniprot.org/uniprot/CKX1_MAIZE CKX1_MAIZE]] Catalyzes the oxidation of cytokinins, a family of N(6)-substituted adenine derivatives that are plant hormones, where the substituent is an isopentenyl group. Cleaves zeatin, isopentenyladenine, isopentenyladenosine, zeatin riboside and cis-zeatin, but not dihydrozeatin, kinetin and benzylaminopurine.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cytokinins are hormones that regulate plant development and their environmental responses. Their levels are mainly controlled by the cytokinin oxidase/dehydrogenase (CKO), which oxidatively cleaves cytokinins using redox-active electron acceptors. CKO belongs to the group of flavoproteins with an 8alpha-N1-histidyl FAD covalent linkage. Here, we investigated the role of seven active site residues: H105, D169, E288, V378, E381, P427 and L492, in substrate binding and catalysis of the CKO1 from maize (Zea mays, ZmCKO1) combining site-directed mutagenesis with kinetics and X-ray crystallography. Here, we identify E381 as a key residue for enzyme specificity that restricts substrate binding as well as quinone electron acceptor binding. We show that D169 is important for catalysis and that H105 covalently linked to FAD maintains the enzyme's structural integrity, stability and high rates with electron acceptors. The L492A mutation significantly modulates the cleavage of aromatic cytokinins and zeatin isomers. The high resolution X-ray structures of ZmCKO1 and the E381S variant in complex with N6-(2-isopentenyl)adenosine (iPR) reveal the binding mode of cytokinin ribosides. Those of ZmCKO2 and ZmCKO4a contain a mobile domain, which might contribute to binding of the N9 substituted cytokinins. This article is protected by copyright. All rights reserved.
		+
		+	Kinetic and structural investigation of the cytokinin oxidase/dehydrogenase active site.,Kopecny D, Koncitikova R, Popelka H, Briozzo P, Vigouroux A, Kopecna M, Zalabak D, Sebela M, Skopalova J, Frebort I, Morera S FEBS J. 2015 Oct 31. doi: 10.1111/febs.13581. PMID:26519657<ref>PMID:26519657</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 3s1f" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 21:08, 30 November 2015

OCA — Mon, 30 Nov 2015 21:08:40 GMT

←Older revision		Revision as of 21:08, 30 November 2015
Line 2:		Line 2:
	<StructureSection load='3s1f' size='340' side='right' caption='[[3s1f]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='3s1f' size='340' side='right' caption='[[3s1f]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3s1f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Zea_mays Zea mays~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S1F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3S1F FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3s1f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Maize Maize]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S1F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3S1F FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=ZIP:N-(3-METHYLBUT-2-EN-1-YL)-9H-PURIN-6-AMINE'>ZIP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=ZIP:N-(3-METHYLBUT-2-EN-1-YL)-9H-PURIN-6-AMINE'>ZIP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bw7\|3bw7]], [[3c0p\|3c0p]], [[2qkn\|2qkn]], [[1w1q\|1w1q]], [[1w1r\|1w1r]], [[1w1s\|1w1s]], [[3dq0\|3dq0]], [[2qpm\|2qpm]], [[3kjm\|3kjm]], [[3s1c\|3s1c]], [[3s1d\|3s1d]], [[3s1e\|3s1e]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bw7\|3bw7]], [[3c0p\|3c0p]], [[2qkn\|2qkn]], [[1w1q\|1w1q]], [[1w1r\|1w1r]], [[1w1s\|1w1s]], [[3dq0\|3dq0]], [[2qpm\|2qpm]], [[3kjm\|3kjm]], [[3s1c\|3s1c]], [[3s1d\|3s1d]], [[3s1e\|3s1e]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CKX1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4577 ~~Zea mays~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CKX1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4577 MAIZE])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cytokinin_dehydrogenase Cytokinin dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.99.12 1.5.99.12] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cytokinin_dehydrogenase Cytokinin dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.99.12 1.5.99.12] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s1f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s1f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3s1f RCSB], [http://www.ebi.ac.uk/pdbsum/3s1f PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s1f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s1f OCA], [http://pdbe.org/3s1f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3s1f RCSB], [http://www.ebi.ac.uk/pdbsum/3s1f PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 14:
	</StructureSection>		</StructureSection>
	[[Category: Cytokinin dehydrogenase]]		[[Category: Cytokinin dehydrogenase]]
-	[[Category: ~~Zea mays~~]]	+	[[Category: Maize]]
	[[Category: Briozzo, P]]		[[Category: Briozzo, P]]
	[[Category: Kopecny, D]]		[[Category: Kopecny, D]]

OCA at 17:25, 25 December 2014

OCA — Thu, 25 Dec 2014 17:25:40 GMT

←Older revision		Revision as of 17:25, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3s1f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S1F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3S1F FirstGlance]. <br>		<table><tr><td colspan='2'>[[3s1f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S1F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3S1F FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=ZIP:N-(3-METHYLBUT-2-EN-1-YL)-9H-PURIN-6-AMINE'>ZIP</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=ZIP:N-(3-METHYLBUT-2-EN-1-YL)-9H-PURIN-6-AMINE'>ZIP</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bw7\|3bw7]], [[3c0p\|3c0p]], [[2qkn\|2qkn]], [[1w1q\|1w1q]], [[1w1r\|1w1r]], [[1w1s\|1w1s]], [[3dq0\|3dq0]], [[2qpm\|2qpm]], [[3kjm\|3kjm]], [[3s1c\|3s1c]], [[3s1d\|3s1d]], [[3s1e\|3s1e]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bw7\|3bw7]], [[3c0p\|3c0p]], [[2qkn\|2qkn]], [[1w1q\|1w1q]], [[1w1r\|1w1r]], [[1w1s\|1w1s]], [[3dq0\|3dq0]], [[2qpm\|2qpm]], [[3kjm\|3kjm]], [[3s1c\|3s1c]], [[3s1d\|3s1d]], [[3s1e\|3s1e]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CKX1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4577 Zea mays])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CKX1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4577 Zea mays])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cytokinin_dehydrogenase Cytokinin dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.99.12 1.5.99.12] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cytokinin_dehydrogenase Cytokinin dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.99.12 1.5.99.12] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s1f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s1f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3s1f RCSB], [http://www.ebi.ac.uk/pdbsum/3s1f PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s1f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s1f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3s1f RCSB], [http://www.ebi.ac.uk/pdbsum/3s1f PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CKX1_MAIZE CKX1_MAIZE]] Catalyzes the oxidation of cytokinins, a family of N(6)-substituted adenine derivatives that are plant hormones, where the substituent is an isopentenyl group. Cleaves zeatin, isopentenyladenine, isopentenyladenosine, zeatin riboside and cis-zeatin, but not dihydrozeatin, kinetin and benzylaminopurine.
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Cytokinin dehydrogenase]]		[[Category: Cytokinin dehydrogenase]]
	[[Category: Zea mays]]		[[Category: Zea mays]]
-	[[Category: Briozzo, P.]]	+	[[Category: Briozzo, P]]
-	[[Category: Kopecny, D.]]	+	[[Category: Kopecny, D]]
-	[[Category: Morera, S.]]	+	[[Category: Morera, S]]
	[[Category: Covalent flavination]]		[[Category: Covalent flavination]]
	[[Category: Cytokinin binding]]		[[Category: Cytokinin binding]]

OCA at 04:47, 5 June 2014

OCA — Thu, 05 Jun 2014 04:47:12 GMT

←Older revision		Revision as of 04:47, 5 June 2014
Line 1:		Line 1:
-	~~[[Image:3s1f.jpg\|left\|200px]]~~	+	==Asp169Glu mutant of maize cytokinin oxidase/dehydrogenase complexed with N6-isopentenyladenine==
-		+	<StructureSection load='3s1f' size='340' side='right' caption='[[3s1f]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~<!--~~	+	== Structural highlights ==
-	~~The line below this paragraph, containing "STRUCTURE_3s1f", creates the "Structure Box" on the page.~~	+	<table><tr><td colspan='2'>[[3s1f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S1F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3S1F FirstGlance]. <br>
-	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~	+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=ZIP:N-(3-METHYLBUT-2-EN-1-YL)-9H-PURIN-6-AMINE'>ZIP</scene><br>
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bw7\|3bw7]], [[3c0p\|3c0p]], [[2qkn\|2qkn]], [[1w1q\|1w1q]], [[1w1r\|1w1r]], [[1w1s\|1w1s]], [[3dq0\|3dq0]], [[2qpm\|2qpm]], [[3kjm\|3kjm]], [[3s1c\|3s1c]], [[3s1d\|3s1d]], [[3s1e\|3s1e]]</td></tr>
-	~~or leave the SCENE parameter empty for the default display.~~	+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CKX1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4577 Zea mays])</td></tr>
-	~~-->~~	+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cytokinin_dehydrogenase Cytokinin dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.99.12 1.5.99.12] </span></td></tr>
-	~~{{STRUCTURE_3s1f\| PDB=3s1f \| SCENE= }}~~	+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s1f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s1f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3s1f RCSB], [http://www.ebi.ac.uk/pdbsum/3s1f PDBsum]</span></td></tr>
-		+	<table>
-	===Asp169Glu mutant of maize cytokinin oxidase/dehydrogenase complexed with N6-isopentenyladenine===	+	__TOC__
-		+	</StructureSection>
-		+
-	==~~About this Structure~~==	+
-	[[3s1f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S1F OCA].	+
	[[Category: Cytokinin dehydrogenase]]		[[Category: Cytokinin dehydrogenase]]
	[[Category: Zea mays]]		[[Category: Zea mays]]

OCA at 06:10, 23 May 2012

OCA — Wed, 23 May 2012 06:10:01 GMT

←Older revision		Revision as of 06:10, 23 May 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3s1f.jpg\|left\|200px]]

-	The ~~entry 3s1f~~ is ~~ON HOLD~~ ~~until May 20 2013~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3s1f", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3s1f\| PDB=3s1f \| SCENE= }}

-	~~Authors: Kopecny, D., Briozzo, P., Morera, S.~~	+	===Asp169Glu mutant of maize cytokinin oxidase/dehydrogenase complexed with N6-isopentenyladenine===

-	~~Description~~: ~~Asp169Glu mutant of maize cytokinin~~ oxidase/dehydrogenase ~~complexed with N6-isopentenyladenine~~	+
		+	==About this Structure==
		+	[[3s1f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Zea_mays Zea mays]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S1F OCA].
		+	[[Category: Cytokinin dehydrogenase]]
		+	[[Category: Zea mays]]
		+	[[Category: Briozzo, P.]]
		+	[[Category: Kopecny, D.]]
		+	[[Category: Morera, S.]]
		+	[[Category: Covalent flavination]]
		+	[[Category: Cytokinin binding]]
		+	[[Category: Cytokinin oxidase/dehydrogenase]]
		+	[[Category: Fad binding protein]]
		+	[[Category: Flavoprotein]]
		+	[[Category: Glycosylation]]
		+	[[Category: Oxidoreductase]]

OCA at 08:33, 16 May 2012

OCA — Wed, 16 May 2012 08:33:56 GMT

←Older revision		Revision as of 08:33, 16 May 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3s1f is ON HOLD until ~~Paper Publication~~	+	The entry 3s1f is ON HOLD until May 20 2013

	Authors: Kopecny, D., Briozzo, P., Morera, S.		Authors: Kopecny, D., Briozzo, P., Morera, S.

	Description: Asp169Glu mutant of maize cytokinin oxidase/dehydrogenase complexed with N6-isopentenyladenine		Description: Asp169Glu mutant of maize cytokinin oxidase/dehydrogenase complexed with N6-isopentenyladenine