3s61 - Revision history

OCA at 09:47, 1 March 2024

OCA — Fri, 01 Mar 2024 09:47:49 GMT

←Older revision		Revision as of 09:47, 1 March 2024
Line 3:		Line 3:
	<StructureSection load='3s61' size='340' side='right'caption='[[3s61]], [[Resolution\|resolution]] 3.03Å' scene=''>		<StructureSection load='3s61' size='340' side='right'caption='[[3s61]], [[Resolution\|resolution]] 3.03Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3s61]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S61 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3S61 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3s61]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S61 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3S61 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=FAD:FLAVIN~~-~~ADENINE+DINUCLEOTIDE'>FAD</scene>~~, ~~<scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=ORN:L-ORNITHINE'>ORN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.03Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[3s5w\|3s5w]]~~</~~div~~><~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=ORN:L-ORNITHINE'>ORN</scene></td></tr>
-	<~~tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">PA2386, pvd-1, pvdA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3s61 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s61 OCA], [https://pdbe.org/3s61 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3s61 RCSB], [https://www.ebi.ac.uk/pdbsum/3s61 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3s61 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3s61 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s61 OCA], [https://pdbe.org/3s61 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3s61 RCSB], [https://www.ebi.ac.uk/pdbsum/3s61 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3s61 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/PVDA_PSEAE PVDA_PSEAE]] Catalyzes the conversion of L-ornithine to N(5)-hydroxyornithine, the first step in the biosynthesis of all hydroxamate-containing siderophores, such as pyoverdin. Pyoverdin is a hydroxamate siderophore composed of a 6,7-dihydroxyquinoline-containing fluorescent chromophore joined to the N-terminus of a partly cyclic octapeptide (D-Ser-L-Arg-D-Ser-L-N(5)-OH-Orn-L-Lys-L-N(5)-OH-Orn-L-Thr-L-Thr in strain PAO1). Specific for NADPH, which plays a role in stabilization of the C4a-hydroperoxyflavin intermediate.<ref>PMID:17015659</ref> <ref>PMID:19368334</ref> <ref>PMID:8106324</ref>	+	[https://www.uniprot.org/uniprot/PVDA_PSEAE PVDA_PSEAE] Catalyzes the conversion of L-ornithine to N(5)-hydroxyornithine, the first step in the biosynthesis of all hydroxamate-containing siderophores, such as pyoverdin. Pyoverdin is a hydroxamate siderophore composed of a 6,7-dihydroxyquinoline-containing fluorescent chromophore joined to the N-terminus of a partly cyclic octapeptide (D-Ser-L-Arg-D-Ser-L-N(5)-OH-Orn-L-Lys-L-N(5)-OH-Orn-L-Thr-L-Thr in strain PAO1). Specific for NADPH, which plays a role in stabilization of the C4a-hydroperoxyflavin intermediate.<ref>PMID:17015659</ref> <ref>PMID:19368334</ref> <ref>PMID:8106324</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The ornithine hydroxylase from Pseudomonas aeruginosa (PvdA) catalyzes the FAD dependent hydroxylation of the sidechain amine of ornithine, which is subsequently formylated to generate the iron chelating hydroxamates of the siderophore pyoverdin. PvdA belongs to the class B flavoprotein monooxygenases, which catalyze the oxidation of substrates using NADPH as electron donor and molecular oxygen. Class B enzymes include the well-studied flavin-containing monooxygenases and Baeyer-Villiger monoogenases. The first two structures of a class B N-hydroxylating monooxygenase were determined with the FAD in oxidized (1.9 A resolution) and reduced states (3.03 A). PvdA has the two expected Rossman-like dinucleotide binding domains for FAD and NADPH, and also a substrate binding domain, with the active site at the interface between the three domains. The structures have NADP(H) and (hydroxy)ornithine bound in a solvent exposed active site, providing structural evidence for substrate and co-substrate specificity and the inability of PvdA to bind FAD tightly. Structural and biochemical evidence indicate that NADP+ remains bound throughout the oxidative half-reaction, which is proposed to shelter the flavin intermediates from solvent and thereby prevent uncoupling of NADPH oxidation from hydroxylated product formation.	+
-		+
-	Two structures of an N-hydroxylating flavoprotein monooxgenase: the ornithine hydroxylase from Pseudomonas aeruginosa.,Olucha J, Meneely KM, Chilton AS, Lamb AL J Biol Chem. 2011 Jul 13. PMID:21757711<ref>PMID:21757711</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3s61" style="background-color:#fffaf0;"></div~~>	+

	==See Also==		==See Also==
Line 28:		Line 18:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lamb, A L~~]]	+	[[Category: Pseudomonas aeruginosa]]
-	[[Category: ~~Olucha, J~~]]	+	[[Category: Lamb AL]]
-	[[Category: ~~Bacterial cytosol]]~~	+	[[Category: Olucha J]]
-	~~[[Category: Class b flavin dependent n-hydroxylating monooxygenase]]~~	+
-	~~[[Category: N5-l-ornithine monooxygenase]]~~	+
-	~~[[Category: Ornithine hydroxylase]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Reduced form~~]]	+

OCA at 10:39, 22 June 2022

OCA — Wed, 22 Jun 2022 10:39:39 GMT

←Older revision		Revision as of 10:39, 22 June 2022
Line 1:		Line 1:

	==Reduced Form of Ornithine Hydroxylase (PvdA) from Pseudomonas aeruginosa==		==Reduced Form of Ornithine Hydroxylase (PvdA) from Pseudomonas aeruginosa==
-	<StructureSection load='3s61' size='340' side='right' caption='[[3s61]], [[Resolution\|resolution]] 3.03Å' scene=''>	+	<StructureSection load='3s61' size='340' side='right'caption='[[3s61]], [[Resolution\|resolution]] 3.03Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3s61]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S61 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3S61 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3s61]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S61 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3S61 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=ORN:L-ORNITHINE'>ORN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=ORN:L-ORNITHINE'>ORN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3s5w\|3s5w]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3s5w\|3s5w]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PA2386, pvd-1, pvdA ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PA2386, pvd-1, pvdA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3s61 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s61 OCA], [~~http~~://pdbe.org/3s61 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3s61 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3s61 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3s61 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3s61 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s61 OCA], [https://pdbe.org/3s61 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3s61 RCSB], [https://www.ebi.ac.uk/pdbsum/3s61 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3s61 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PVDA_PSEAE PVDA_PSEAE]] Catalyzes the conversion of L-ornithine to N(5)-hydroxyornithine, the first step in the biosynthesis of all hydroxamate-containing siderophores, such as pyoverdin. Pyoverdin is a hydroxamate siderophore composed of a 6,7-dihydroxyquinoline-containing fluorescent chromophore joined to the N-terminus of a partly cyclic octapeptide (D-Ser-L-Arg-D-Ser-L-N(5)-OH-Orn-L-Lys-L-N(5)-OH-Orn-L-Thr-L-Thr in strain PAO1). Specific for NADPH, which plays a role in stabilization of the C4a-hydroperoxyflavin intermediate.<ref>PMID:17015659</ref> <ref>PMID:19368334</ref> <ref>PMID:8106324</ref>	+	[[https://www.uniprot.org/uniprot/PVDA_PSEAE PVDA_PSEAE]] Catalyzes the conversion of L-ornithine to N(5)-hydroxyornithine, the first step in the biosynthesis of all hydroxamate-containing siderophores, such as pyoverdin. Pyoverdin is a hydroxamate siderophore composed of a 6,7-dihydroxyquinoline-containing fluorescent chromophore joined to the N-terminus of a partly cyclic octapeptide (D-Ser-L-Arg-D-Ser-L-N(5)-OH-Orn-L-Lys-L-N(5)-OH-Orn-L-Thr-L-Thr in strain PAO1). Specific for NADPH, which plays a role in stabilization of the C4a-hydroperoxyflavin intermediate.<ref>PMID:17015659</ref> <ref>PMID:19368334</ref> <ref>PMID:8106324</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 3s61" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3s61" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Monooxygenase 3D structures\|Monooxygenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Lamb, A L]]		[[Category: Lamb, A L]]
	[[Category: Olucha, J]]		[[Category: Olucha, J]]

OCA at 08:19, 11 August 2016

OCA — Thu, 11 Aug 2016 08:19:04 GMT

←Older revision		Revision as of 08:19, 11 August 2016
Line 1:		Line 1:
		+
	==Reduced Form of Ornithine Hydroxylase (PvdA) from Pseudomonas aeruginosa==		==Reduced Form of Ornithine Hydroxylase (PvdA) from Pseudomonas aeruginosa==
	<StructureSection load='3s61' size='340' side='right' caption='[[3s61]], [[Resolution\|resolution]] 3.03Å' scene=''>		<StructureSection load='3s61' size='340' side='right' caption='[[3s61]], [[Resolution\|resolution]] 3.03Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3s61]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_aeruginosa Pseudomonas aeruginosa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S61 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3S61 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3s61]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S61 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3S61 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=ORN:L-ORNITHINE'>ORN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=ORN:L-ORNITHINE'>ORN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3s5w\|3s5w]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3s5w\|3s5w]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PA2386, pvd-1, pvdA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 ~~Pseudomonas aeruginosa~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PA2386, pvd-1, pvdA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s61 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s61 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3s61 RCSB], [http://www.ebi.ac.uk/pdbsum/3s61 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s61 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s61 OCA], [http://pdbe.org/3s61 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3s61 RCSB], [http://www.ebi.ac.uk/pdbsum/3s61 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3s61 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PVDA_PSEAE PVDA_PSEAE]] Catalyzes the conversion of L-ornithine to N(5)-hydroxyornithine, the first step in the biosynthesis of all hydroxamate-containing siderophores, such as pyoverdin. Pyoverdin is a hydroxamate siderophore composed of a 6,7-dihydroxyquinoline-containing fluorescent chromophore joined to the N-terminus of a partly cyclic octapeptide (D-Ser-L-Arg-D-Ser-L-N(5)-OH-Orn-L-Lys-L-N(5)-OH-Orn-L-Thr-L-Thr in strain PAO1). Specific for NADPH, which plays a role in stabilization of the C4a-hydroperoxyflavin intermediate.<ref>PMID:17015659</ref> <ref>PMID:19368334</ref> <ref>PMID:8106324</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 16:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3s61" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Pseudomonas aeruginosa]]
	[[Category: Lamb, A L]]		[[Category: Lamb, A L]]
	[[Category: Olucha, J]]		[[Category: Olucha, J]]

OCA at 10:26, 4 January 2015

OCA — Sun, 04 Jan 2015 10:26:59 GMT

←Older revision		Revision as of 10:26, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3s61]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S61 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3S61 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3s61]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S61 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3S61 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=ORN:L-ORNITHINE'>ORN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=ORN:L-ORNITHINE'>ORN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3s5w\|3s5w]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3s5w\|3s5w]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PA2386, pvd-1, pvdA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PA2386, pvd-1, pvdA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s61 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s61 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3s61 RCSB], [http://www.ebi.ac.uk/pdbsum/3s61 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s61 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s61 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3s61 RCSB], [http://www.ebi.ac.uk/pdbsum/3s61 PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 14:
	Two structures of an N-hydroxylating flavoprotein monooxgenase: the ornithine hydroxylase from Pseudomonas aeruginosa.,Olucha J, Meneely KM, Chilton AS, Lamb AL J Biol Chem. 2011 Jul 13. PMID:21757711<ref>PMID:21757711</ref>		Two structures of an N-hydroxylating flavoprotein monooxgenase: the ornithine hydroxylase from Pseudomonas aeruginosa.,Olucha J, Meneely KM, Chilton AS, Lamb AL J Biol Chem. 2011 Jul 13. PMID:21757711<ref>PMID:21757711</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
-	[[Category: Lamb, A L.]]	+	[[Category: Lamb, A L]]
-	[[Category: Olucha, J.]]	+	[[Category: Olucha, J]]
	[[Category: Bacterial cytosol]]		[[Category: Bacterial cytosol]]
	[[Category: Class b flavin dependent n-hydroxylating monooxygenase]]		[[Category: Class b flavin dependent n-hydroxylating monooxygenase]]

OCA at 05:24, 5 June 2014

OCA — Thu, 05 Jun 2014 05:24:07 GMT

←Older revision		Revision as of 05:24, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3s61.~~png~~\|~~left~~\|~~200px~~]]	+	==Reduced Form of Ornithine Hydroxylase (PvdA) from Pseudomonas aeruginosa==
		+	<StructureSection load='3s61' size='340' side='right' caption='[[3s61]], [[Resolution\|resolution]] 3.03Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3s61]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S61 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3S61 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene>, <scene name='pdbligand=ORN:L-ORNITHINE'>ORN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3s5w\|3s5w]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">PA2386, pvd-1, pvdA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s61 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s61 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3s61 RCSB], [http://www.ebi.ac.uk/pdbsum/3s61 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The ornithine hydroxylase from Pseudomonas aeruginosa (PvdA) catalyzes the FAD dependent hydroxylation of the sidechain amine of ornithine, which is subsequently formylated to generate the iron chelating hydroxamates of the siderophore pyoverdin. PvdA belongs to the class B flavoprotein monooxygenases, which catalyze the oxidation of substrates using NADPH as electron donor and molecular oxygen. Class B enzymes include the well-studied flavin-containing monooxygenases and Baeyer-Villiger monoogenases. The first two structures of a class B N-hydroxylating monooxygenase were determined with the FAD in oxidized (1.9 A resolution) and reduced states (3.03 A). PvdA has the two expected Rossman-like dinucleotide binding domains for FAD and NADPH, and also a substrate binding domain, with the active site at the interface between the three domains. The structures have NADP(H) and (hydroxy)ornithine bound in a solvent exposed active site, providing structural evidence for substrate and co-substrate specificity and the inability of PvdA to bind FAD tightly. Structural and biochemical evidence indicate that NADP+ remains bound throughout the oxidative half-reaction, which is proposed to shelter the flavin intermediates from solvent and thereby prevent uncoupling of NADPH oxidation from hydroxylated product formation.

-	<!--	+	Two structures of an N-hydroxylating flavoprotein monooxgenase: the ornithine hydroxylase from Pseudomonas aeruginosa.,Olucha J, Meneely KM, Chilton AS, Lamb AL J Biol Chem. 2011 Jul 13. PMID:21757711<ref>PMID:21757711</ref>
-	~~The line below this paragraph, containing "STRUCTURE_3s61", creates~~ the ~~"Structure Box" on the page~~.	+
-	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded)~~,	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3s61\| PDB=3s61 \| SCENE= }}~~	+

-	~~===Reduced Form~~ of ~~Ornithine Hydroxylase (PvdA) from Pseudomonas aeruginosa===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_21757711}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 21757711 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_21757711}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3s61]] is a 2 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S61 OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:021757711<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
	[[Category: Lamb, A L.]]		[[Category: Lamb, A L.]]

OCA at 05:02, 27 July 2011

OCA — Wed, 27 Jul 2011 05:02:45 GMT

←Older revision		Revision as of 05:02, 27 July 2011
Line 1:		Line 1:
-	[[Image:3s61.~~jpg~~\|left\|200px]]	+	[[Image:3s61.png\|left\|200px]]

	<!--		<!--
Line 11:		Line 11:
	===Reduced Form of Ornithine Hydroxylase (PvdA) from Pseudomonas aeruginosa===		===Reduced Form of Ornithine Hydroxylase (PvdA) from Pseudomonas aeruginosa===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21757711}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21757711 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21757711}}

	==About this Structure==		==About this Structure==
	[[3s61]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S61 OCA].		[[3s61]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S61 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021757711</ref><references group="xtra"/>
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
	[[Category: Lamb, A L.]]		[[Category: Lamb, A L.]]

OCA at 07:51, 13 July 2011

OCA — Wed, 13 Jul 2011 07:51:50 GMT

←Older revision		Revision as of 07:51, 13 July 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3s61.jpg\|left\|200px]]

-	The ~~entry 3s61~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3s61", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3s61\| PDB=3s61 \| SCENE= }}

-	~~Authors: Olucha, J., Lamb, A.L.~~	+	===Reduced Form of Ornithine Hydroxylase (PvdA) from Pseudomonas aeruginosa===

-	~~Description~~: ~~Reduced Form of Ornithine Hydroxylase (PvdA)~~ from Pseudomonas aeruginosa	+
		+	==About this Structure==
		+	[[3s61]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S61 OCA].
		+	[[Category: Pseudomonas aeruginosa]]
		+	[[Category: Lamb, A L.]]
		+	[[Category: Olucha, J.]]
		+	[[Category: Bacterial cytosol]]
		+	[[Category: Class b flavin dependent n-hydroxylating monooxygenase]]
		+	[[Category: N5-l-ornithine monooxygenase]]
		+	[[Category: Ornithine hydroxylase]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Reduced form]]

OCA at 05:54, 15 June 2011

OCA — Wed, 15 Jun 2011 05:54:39 GMT

←Older revision		Revision as of 05:54, 15 June 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3s61 is ON HOLD	+	The entry 3s61 is ON HOLD until Paper Publication

-	Authors: Olucha, J., Lamb, A. L.	+	Authors: Olucha, J., Lamb, A.L.

	Description: Reduced Form of Ornithine Hydroxylase (PvdA) from Pseudomonas aeruginosa		Description: Reduced Form of Ornithine Hydroxylase (PvdA) from Pseudomonas aeruginosa

OCA: Protected "3s61" [edit=sysop:move=sysop]

OCA — Wed, 01 Jun 2011 05:27:35 GMT

Protected "3s61" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:27, 1 June 2011

OCA: New page: '''Unreleased structure''' The entry 3s61 is ON HOLD Authors: Olucha, J., Lamb, A. L. Description: Reduced Form of Ornithine Hydroxylase (PvdA) from Pseudomonas aeruginosa

OCA — Wed, 01 Jun 2011 05:27:34 GMT

New page: '''Unreleased structure''' The entry 3s61 is ON HOLD Authors: Olucha, J., Lamb, A. L. Description: Reduced Form of Ornithine Hydroxylase (PvdA) from Pseudomonas aeruginosa

New page

'''Unreleased structure'''

The entry 3s61 is ON HOLD

Authors: Olucha, J., Lamb, A. L.

Description: Reduced Form of Ornithine Hydroxylase (PvdA) from Pseudomonas aeruginosa