3s6n - Revision history

OCA: Redirecting to 5xjl

OCA — Wed, 02 May 2018 07:52:58 GMT

Redirecting to 5xjl

←Older revision		Revision as of 07:52, 2 May 2018
Line 1:		Line 1:
-		+	#REDIRECT [[5xjl]] This PDB entry is obsolete and replaced by 5xjl
-	~~==Crystal Structure of the Gemin2-binding domain of SMN, Gemin2 in Complex with SmD1/D2/F/E/G from Human==~~	+
-	~~<StructureSection load='3s6n' size='340' side='right' caption='~~[[~~3s6n]], [[Resolution\|resolution]] 2.50Å' scene=''>~~	+
-	~~== Structural highlights ==~~	+
-	~~<table><tr><td colspan='2'>[[3s6n~~]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S6N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3S6N FirstGlance]. <br>	+
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SIP1, GEMIN2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), SNRPD1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), SNRPD2, SNRPD1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), SNRPE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), SNRPF, PBSCF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), SNRPG, PBSCG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), SMN1, SMN, SMNT, SMN2, SMNC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s6n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s6n OCA], [http://pdbe.org/3s6n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3s6n RCSB], [http://www.ebi.ac.uk/pdbsum/3s6n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3s6n ProSAT]</span></td></tr>	+
-	~~</table>~~	+
-	~~== Function ==~~	+
-	~~[[http://www.uniprot.org/uniprot/RUXG_HUMAN RUXG_HUMAN]] Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Associated with snRNP U1, U2, U4/U6~~ and U5. [[http://www.uniprot.org/uniprot/RUXE_HUMAN RUXE_HUMAN]] Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Associated with snRNP U1, U2, U4/U6 and U5. [[http://www.uniprot.org/uniprot/GEMI2_HUMAN GEMI2_HUMAN]] The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus.<ref>PMID:18984161</ref> <ref>PMID:9323129</ref> [[http://www.uniprot.org/uniprot/SMD2_HUMAN SMD2_HUMAN]] Required for pre-mRNA splicing. Required for snRNP biogenesis (By similarity). [[http://www.uniprot.org/uniprot/SMD1_HUMAN SMD1_HUMAN]] May act as a charged protein scaffold to promote snRNP assembly or strengthen snRNP-snRNP interactions through nonspecific electrostatic contacts with RNA. [[http://www.uniprot.org/uniprot/RUXF_HUMAN RUXF_HUMAN]] Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Associated with snRNP U1, U2, U4/U6 and U5.	+
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The SMN complex mediates the assembly of heptameric Sm protein rings on small nuclear RNAs (snRNAs), which are essential for snRNP function. Specific Sm core assembly depends on Sm proteins and snRNA recognition by SMN/Gemin2- and Gemin5-containing subunits, respectively. The mechanism by which the Sm proteins are gathered while preventing illicit Sm assembly on non-snRNAs is unknown. Here, we describe the 2.5 A crystal structure of Gemin2 bound to SmD1/D2/F/E/G pentamer and SMN's Gemin2-binding domain, a key assembly intermediate. Remarkably, through its extended conformation, Gemin2 wraps around the crescent-shaped pentamer, interacting with all five Sm proteins, and gripping its bottom and top sides and outer perimeter. Gemin2 reaches into the RNA-binding pocket, preventing RNA binding. Interestingly, SMN-Gemin2 interaction is abrogated by a spinal muscular atrophy (SMA)-causing mutation in an SMN helix that mediates Gemin2 binding. These findings provide insight into SMN complex assembly and specificity, linking snRNP biogenesis and SMA pathogenesis.	+
-		+
-	Structure of a Key Intermediate of the SMN Complex Reveals Gemin2's Crucial Function in snRNP Assembly.,Zhang R, So BR, Li P, Yong J, Glisovic T, Wan L, Dreyfuss G Cell. 2011 Aug 5;146(3):384-95. PMID:21816274<ref>PMID:21816274</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3s6n" style="background-color:#fffaf0;"></div>~~	+
-		+
-	~~==See Also==~~	+
-	*[[Nucleoprotein\|Nucleoprotein]]	+
-	~~== References ==~~	+
-	~~<references/>~~	+
-	~~__TOC__~~	+
-	~~</StructureSection>~~	+
-	~~[[Category: Human]]~~	+
-	~~[[Category: Dreyfuss, G]]~~	+
-	~~[[Category: Glisovic, T]]~~	+
-	~~[[Category: Li, P]]~~	+
-	~~[[Category: So, B R]]~~	+
-	~~[[Category: Wan, L]]~~	+
-	~~[[Category: Yong, J]]~~	+
-	~~[[Category: Zhang, R]]~~	+
-	~~[[Category: Helix protein]]~~	+
-	~~[[Category: Heteromeric heptameric ring]]~~	+
-	~~[[Category: Mrna]]~~	+
-	~~[[Category: Pre-mrna splicing]]~~	+
-	~~[[Category: Protein complex]]~~	+
-	~~[[Category: Rna binding]]~~	+
-	~~[[Category: Sm core]]~~	+
-	~~[[Category: Sm fold]]~~	+
-	~~[[Category: Smn complex]]~~	+
-	~~[[Category: Smn-gemin2 complex]]~~	+
-	~~[[Category: Snrnp biogenesis]]~~	+
-	~~[[Category: Snrnp]]~~	+
-	~~[[Category: Spliceosome]]~~	+
-	~~[[Category: Splicing]]~~	+
-	~~[[Category: Splicing factor]]~~	+
-	~~[[Category: Survival of motor neuron]]~~	+
-	~~[[Category: U-rich snrna]]~~	+

OCA at 06:58, 5 August 2016

OCA — Fri, 05 Aug 2016 06:58:45 GMT

←Older revision		Revision as of 06:58, 5 August 2016
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		+
	==Crystal Structure of the Gemin2-binding domain of SMN, Gemin2 in Complex with SmD1/D2/F/E/G from Human==		==Crystal Structure of the Gemin2-binding domain of SMN, Gemin2 in Complex with SmD1/D2/F/E/G from Human==
	<StructureSection load='3s6n' size='340' side='right' caption='[[3s6n]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3s6n' size='340' side='right' caption='[[3s6n]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3s6n]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S6N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3S6N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3s6n]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S6N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3S6N FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SIP1, GEMIN2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~]), SNRPD1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~]), SNRPD2, SNRPD1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~]), SNRPE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~]), SNRPF, PBSCF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~]), SNRPG, PBSCG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~]), SMN1, SMN, SMNT, SMN2, SMNC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SIP1, GEMIN2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), SNRPD1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), SNRPD2, SNRPD1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), SNRPE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), SNRPF, PBSCF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), SNRPG, PBSCG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), SMN1, SMN, SMNT, SMN2, SMNC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s6n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s6n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3s6n RCSB], [http://www.ebi.ac.uk/pdbsum/3s6n PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s6n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s6n OCA], [http://pdbe.org/3s6n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3s6n RCSB], [http://www.ebi.ac.uk/pdbsum/3s6n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3s6n ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/RUXG_HUMAN RUXG_HUMAN]] Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Associated with snRNP U1, U2, U4/U6 and U5. [[http://www.uniprot.org/uniprot/RUXE_HUMAN RUXE_HUMAN]] Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Associated with snRNP U1, U2, U4/U6 and U5. [[http://www.uniprot.org/uniprot/SMD2_HUMAN SMD2_HUMAN]] Required for pre-mRNA splicing. Required for snRNP biogenesis (By similarity). [[http://www.uniprot.org/uniprot/SMD1_HUMAN SMD1_HUMAN]] May act as a charged protein scaffold to promote snRNP assembly or strengthen snRNP-snRNP interactions through nonspecific electrostatic contacts with RNA. [[http://www.uniprot.org/uniprot/RUXF_HUMAN RUXF_HUMAN]] Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Associated with snRNP U1, U2, U4/U6 and U5.	+	[[http://www.uniprot.org/uniprot/RUXG_HUMAN RUXG_HUMAN]] Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Associated with snRNP U1, U2, U4/U6 and U5. [[http://www.uniprot.org/uniprot/RUXE_HUMAN RUXE_HUMAN]] Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Associated with snRNP U1, U2, U4/U6 and U5. [[http://www.uniprot.org/uniprot/GEMI2_HUMAN GEMI2_HUMAN]] The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus.<ref>PMID:18984161</ref> <ref>PMID:9323129</ref> [[http://www.uniprot.org/uniprot/SMD2_HUMAN SMD2_HUMAN]] Required for pre-mRNA splicing. Required for snRNP biogenesis (By similarity). [[http://www.uniprot.org/uniprot/SMD1_HUMAN SMD1_HUMAN]] May act as a charged protein scaffold to promote snRNP assembly or strengthen snRNP-snRNP interactions through nonspecific electrostatic contacts with RNA. [[http://www.uniprot.org/uniprot/RUXF_HUMAN RUXF_HUMAN]] Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Associated with snRNP U1, U2, U4/U6 and U5.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3s6n" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 23:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Dreyfuss, G]]		[[Category: Dreyfuss, G]]
	[[Category: Glisovic, T]]		[[Category: Glisovic, T]]

OCA at 16:41, 25 December 2014

OCA — Thu, 25 Dec 2014 16:41:38 GMT

←Older revision		Revision as of 16:41, 25 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s6n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s6n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3s6n RCSB], [http://www.ebi.ac.uk/pdbsum/3s6n PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s6n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s6n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3s6n RCSB], [http://www.ebi.ac.uk/pdbsum/3s6n PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RUXG_HUMAN RUXG_HUMAN]] Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Associated with snRNP U1, U2, U4/U6 and U5. [[http://www.uniprot.org/uniprot/RUXE_HUMAN RUXE_HUMAN]] Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Associated with snRNP U1, U2, U4/U6 and U5. [[http://www.uniprot.org/uniprot/SMD2_HUMAN SMD2_HUMAN]] Required for pre-mRNA splicing. Required for snRNP biogenesis (By similarity). [[http://www.uniprot.org/uniprot/SMD1_HUMAN SMD1_HUMAN]] May act as a charged protein scaffold to promote snRNP assembly or strengthen snRNP-snRNP interactions through nonspecific electrostatic contacts with RNA. [[http://www.uniprot.org/uniprot/RUXF_HUMAN RUXF_HUMAN]] Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Associated with snRNP U1, U2, U4/U6 and U5.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 12:47, 9 December 2014

OCA — Tue, 09 Dec 2014 12:47:28 GMT

←Older revision		Revision as of 12:47, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3s6n.~~png~~\|~~left\|200px~~]]	+	==Crystal Structure of the Gemin2-binding domain of SMN, Gemin2 in Complex with SmD1/D2/F/E/G from Human==
		+	<StructureSection load='3s6n' size='340' side='right' caption='[[3s6n]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3s6n]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S6N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3S6N FirstGlance]. <br>
		+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SIP1, GEMIN2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), SNRPD1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), SNRPD2, SNRPD1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), SNRPE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), SNRPF, PBSCF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), SNRPG, PBSCG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), SMN1, SMN, SMNT, SMN2, SMNC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s6n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s6n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3s6n RCSB], [http://www.ebi.ac.uk/pdbsum/3s6n PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The SMN complex mediates the assembly of heptameric Sm protein rings on small nuclear RNAs (snRNAs), which are essential for snRNP function. Specific Sm core assembly depends on Sm proteins and snRNA recognition by SMN/Gemin2- and Gemin5-containing subunits, respectively. The mechanism by which the Sm proteins are gathered while preventing illicit Sm assembly on non-snRNAs is unknown. Here, we describe the 2.5 A crystal structure of Gemin2 bound to SmD1/D2/F/E/G pentamer and SMN's Gemin2-binding domain, a key assembly intermediate. Remarkably, through its extended conformation, Gemin2 wraps around the crescent-shaped pentamer, interacting with all five Sm proteins, and gripping its bottom and top sides and outer perimeter. Gemin2 reaches into the RNA-binding pocket, preventing RNA binding. Interestingly, SMN-Gemin2 interaction is abrogated by a spinal muscular atrophy (SMA)-causing mutation in an SMN helix that mediates Gemin2 binding. These findings provide insight into SMN complex assembly and specificity, linking snRNP biogenesis and SMA pathogenesis.

-	~~{{STRUCTURE_3s6n\| PDB=3s6n \| SCENE= }}~~	+	Structure of a Key Intermediate of the SMN Complex Reveals Gemin2's Crucial Function in snRNP Assembly.,Zhang R, So BR, Li P, Yong J, Glisovic T, Wan L, Dreyfuss G Cell. 2011 Aug 5;146(3):384-95. PMID:21816274<ref>PMID:21816274</ref>

-	~~===Crystal Structure~~ of the ~~Gemin2-binding domain of SMN, Gemin2 in Complex with SmD1/D2/F/E/G from Human===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_21816274}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3s6n]] is a 7 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3S6N OCA].~~	+

	==See Also==		==See Also==
	*[[Nucleoprotein\|Nucleoprotein]]		*[[Nucleoprotein\|Nucleoprotein]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:021816274<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Dreyfuss, G.]]	+	[[Category: Dreyfuss, G]]
-	[[Category: Glisovic, T.]]	+	[[Category: Glisovic, T]]
-	[[Category: Li, P.]]	+	[[Category: Li, P]]
-	[[Category: So, B R.]]	+	[[Category: So, B R]]
-	[[Category: Wan, L.]]	+	[[Category: Wan, L]]
-	[[Category: Yong, J.]]	+	[[Category: Yong, J]]
-	[[Category: Zhang, R.]]	+	[[Category: Zhang, R]]
	[[Category: Helix protein]]		[[Category: Helix protein]]
	[[Category: Heteromeric heptameric ring]]		[[Category: Heteromeric heptameric ring]]

OCA at 19:01, 20 October 2012

OCA — Sat, 20 Oct 2012 19:01:43 GMT

←Older revision		Revision as of 19:01, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3s6n]] is a 7 chain structure ~~of [[Nucleoprotein]]~~ with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S6N OCA].	+	[[3s6n]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S6N OCA].

	==See Also==		==See Also==

OCA at 06:02, 27 July 2012

OCA — Fri, 27 Jul 2012 06:02:53 GMT

←Older revision		Revision as of 06:02, 27 July 2012
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-	[[Image:3s6n.~~jpg~~\|left\|200px]]	+	[[Image:3s6n.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3s6n", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3s6n\| PDB=3s6n \| SCENE= }}		{{STRUCTURE_3s6n\| PDB=3s6n \| SCENE= }}

	===Crystal Structure of the Gemin2-binding domain of SMN, Gemin2 in Complex with SmD1/D2/F/E/G from Human===		===Crystal Structure of the Gemin2-binding domain of SMN, Gemin2 in Complex with SmD1/D2/F/E/G from Human===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21816274}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 21816274 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_21816274}}		{{ABSTRACT_PUBMED_21816274}}

	==About this Structure==		==About this Structure==
-	[[3s6n]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S6N OCA].	+	[[3s6n]] is a 7 chain structure of [[Nucleoprotein]] with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S6N OCA].
		+
		+	==See Also==
		+	*[[Nucleoprotein\|Nucleoprotein]]

	==Reference==		==Reference==

OCA at 06:14, 17 August 2011

OCA — Wed, 17 Aug 2011 06:14:58 GMT

←Older revision		Revision as of 06:14, 17 August 2011
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-	~~'''Unreleased structure'''~~	+	[[Image:3s6n.jpg\|left\|200px]]

-	The ~~entry 3s6n~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3s6n", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3s6n\| PDB=3s6n \| SCENE= }}

-	~~Authors: Zhang, R., So, B.R., Li, P., Yong, J., Glisovic, T., Wan, L., Dreyfuss~~, G.	+	===Crystal Structure of the Gemin2-binding domain of SMN, Gemin2 in Complex with SmD1/D2/F/E/G from Human===

-	~~Description~~:	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21816274}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21816274 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21816274}}
		+
		+	==About this Structure==
		+	[[3s6n]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S6N OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021816274</ref><references group="xtra"/>
		+	[[Category: Homo sapiens]]
		+	[[Category: Dreyfuss, G.]]
		+	[[Category: Glisovic, T.]]
		+	[[Category: Li, P.]]
		+	[[Category: So, B R.]]
		+	[[Category: Wan, L.]]
		+	[[Category: Yong, J.]]
		+	[[Category: Zhang, R.]]
		+	[[Category: Helix protein]]
		+	[[Category: Heteromeric heptameric ring]]
		+	[[Category: Mrna]]
		+	[[Category: Pre-mrna splicing]]
		+	[[Category: Protein complex]]
		+	[[Category: Rna binding]]
		+	[[Category: Sm core]]
		+	[[Category: Sm fold]]
		+	[[Category: Smn complex]]
		+	[[Category: Smn-gemin2 complex]]
		+	[[Category: Snrnp biogenesis]]
		+	[[Category: Snrnp]]
		+	[[Category: Spliceosome]]
		+	[[Category: Splicing]]
		+	[[Category: Splicing factor]]
		+	[[Category: Survival of motor neuron]]
		+	[[Category: U-rich snrna]]

OCA at 05:37, 27 July 2011

OCA — Wed, 27 Jul 2011 05:37:09 GMT

←Older revision		Revision as of 05:37, 27 July 2011
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3s6n is ON HOLD	+	The entry 3s6n is ON HOLD until Paper Publication

	Authors: Zhang, R., So, B.R., Li, P., Yong, J., Glisovic, T., Wan, L., Dreyfuss, G.		Authors: Zhang, R., So, B.R., Li, P., Yong, J., Glisovic, T., Wan, L., Dreyfuss, G.

	Description:		Description:

OCA at 05:54, 15 June 2011

OCA — Wed, 15 Jun 2011 05:54:54 GMT

←Older revision		Revision as of 05:54, 15 June 2011
Line 5:		Line 5:
	Authors: Zhang, R., So, B.R., Li, P., Yong, J., Glisovic, T., Wan, L., Dreyfuss, G.		Authors: Zhang, R., So, B.R., Li, P., Yong, J., Glisovic, T., Wan, L., Dreyfuss, G.

-	Description: ~~Crystal Structure of the Gemin2-binding domain of SMN,Gemin2 in Complex with SmD1/D2/F/E/G from Human~~	+	Description:

OCA: Protected "3s6n" [edit=sysop:move=sysop]

OCA — Wed, 01 Jun 2011 05:28:12 GMT

Protected "3s6n" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:28, 1 June 2011