3s8y - Revision history

OCA at 12:50, 14 March 2024

OCA — Thu, 14 Mar 2024 12:50:13 GMT

←Older revision		Revision as of 12:50, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3s8y' size='340' side='right'caption='[[3s8y]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3s8y' size='340' side='right'caption='[[3s8y]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3s8y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Dsm_14852 Dsm 14852~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S8Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3S8Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3s8y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oleispira_antarctica Oleispira antarctica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S8Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3S8Y FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[3i6y\|3i6y]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>olei01171 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~188908 DSM 14852])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Arylesterase Arylesterase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.2 3.1.1.2]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3s8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s8y OCA], [https://pdbe.org/3s8y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3s8y RCSB], [https://www.ebi.ac.uk/pdbsum/3s8y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3s8y ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3s8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s8y OCA], [https://pdbe.org/3s8y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3s8y RCSB], [https://www.ebi.ac.uk/pdbsum/3s8y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3s8y ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/D0VWZ4_OLEAN D0VWZ4_OLEAN]
-	The uncharacterized alpha/beta-hydrolase protein OLEI01171 from the psychrophilic marine bacterium Oleispira antarctica belongs to the PF00756 family of putative esterases, which also includes human esterase D. In the present paper we show that purified recombinant OLEI01171 exhibits high esterase activity against the model esterase substrate alpha-naphthyl acetate at 5-30 degrees C with maximal activity at 15-20 degrees C. The esterase activity of OLEI01171 was stimulated 3-8-fold by the addition of chloride or several other anions (0.1-1.0 M). Compared with mesophilic PF00756 esterases, OLEI01171 exhibited a lower overall protein thermostability. Two crystal structures of OLEI01171 were solved at 1.75 and 2.1 A resolution and revealed a classical serine hydrolase catalytic triad and the presence of a chloride or bromide ion bound in the active site close to the catalytic Ser148. Both anions were found to co-ordinate a potential catalytic water molecule located in the vicinity of the catalytic triad His257. The results of the present study suggest that the bound anion perhaps contributes to the polarization of the catalytic water molecule and increases the rate of the hydrolysis of an acyl-enzyme intermediate. Alanine replacement mutagenesis of OLEI01171 identified ten amino acid residues important for esterase activity. The replacement of Asn225 by lysine had no significant effect on the activity or thermostability of OLEI01171, but resulted in a detectable increase of activity at 35-45 degrees C. The present study has provided insight into the molecular mechanisms of activity of a cold-active and anion-activated carboxyl esterase.	+
-		+
-	Structure and activity of the cold-active and anion-activated carboxyl esterase OLEI01171 from the oil-degrading marine bacterium Oleispira antarctica.,Lemak S, Tchigvintsev A, Petit P, Flick R, Singer AU, Brown G, Evdokimova E, Egorova O, Gonzalez CF, Chernikova TN, Yakimov MM, Kube M, Reinhardt R, Golyshin PN, Savchenko A, Yakunin AF Biochem J. 2012 Jul 15;445(2):~~193-203. PMID:22519667<ref>PMID:22519667<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3s8y" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Arylesterase]]
-	[[Category: Dsm 14852]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Dong, A]]	+	[[Category: Oleispira antarctica]]
-	[[Category: Kagan, O]]	+	[[Category: Dong A]]
-	[[Category: Petit, P]]	+	[[Category: Kagan O]]
-	[[Category: Savchenko, A]]	+	[[Category: Petit P]]
-	[[Category: Yakunin~~, A F]]~~	+	[[Category: Savchenko A]]
-	~~[[Category: Hydrolase]]~~	+	[[Category: Yakunin AF]]
-	~~[[Category: Rossmann fold~~]]	+

OCA at 10:43, 22 June 2022

OCA — Wed, 22 Jun 2022 10:43:06 GMT

←Older revision		Revision as of 10:43, 22 June 2022
Line 1:		Line 1:

	==Bromide soaked structure of an esterase from the oil-degrading bacterium Oleispira antarctica==		==Bromide soaked structure of an esterase from the oil-degrading bacterium Oleispira antarctica==
-	<StructureSection load='3s8y' size='340' side='right' caption='[[3s8y]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='3s8y' size='340' side='right'caption='[[3s8y]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3s8y]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Dsm_14852 Dsm 14852]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S8Y OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3S8Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3s8y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dsm_14852 Dsm 14852]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S8Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3S8Y FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i6y\|3i6y]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3i6y\|3i6y]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">olei01171 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=188908 DSM 14852])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">olei01171 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=188908 DSM 14852])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Arylesterase Arylesterase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.2 3.1.1.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Arylesterase Arylesterase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.2 3.1.1.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3s8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s8y OCA], [~~http~~://pdbe.org/3s8y PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3s8y RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3s8y PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3s8y ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3s8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s8y OCA], [https://pdbe.org/3s8y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3s8y RCSB], [https://www.ebi.ac.uk/pdbsum/3s8y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3s8y ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 25:		Line 25:
	[[Category: Arylesterase]]		[[Category: Arylesterase]]
	[[Category: Dsm 14852]]		[[Category: Dsm 14852]]
		+	[[Category: Large Structures]]
	[[Category: Dong, A]]		[[Category: Dong, A]]
	[[Category: Kagan, O]]		[[Category: Kagan, O]]

OCA at 07:47, 11 August 2016

OCA — Thu, 11 Aug 2016 07:47:26 GMT

←Older revision		Revision as of 07:47, 11 August 2016
Line 1:		Line 1:
		+
	==Bromide soaked structure of an esterase from the oil-degrading bacterium Oleispira antarctica==		==Bromide soaked structure of an esterase from the oil-degrading bacterium Oleispira antarctica==
	<StructureSection load='3s8y' size='340' side='right' caption='[[3s8y]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3s8y' size='340' side='right' caption='[[3s8y]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3s8y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Oleispira_antarctica Oleispira antarctica~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S8Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3S8Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3s8y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dsm_14852 Dsm 14852]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S8Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3S8Y FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i6y\|3i6y]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i6y\|3i6y]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">olei01171 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=188908 ~~Oleispira antarctica~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">olei01171 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=188908 DSM 14852])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arylesterase Arylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.2 3.1.1.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arylesterase Arylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.2 3.1.1.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s8y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3s8y RCSB], [http://www.ebi.ac.uk/pdbsum/3s8y PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s8y OCA], [http://pdbe.org/3s8y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3s8y RCSB], [http://www.ebi.ac.uk/pdbsum/3s8y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3s8y ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3s8y" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 22:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Arylesterase]]		[[Category: Arylesterase]]
-	[[Category: ~~Oleispira antarctica~~]]	+	[[Category: Dsm 14852]]
	[[Category: Dong, A]]		[[Category: Dong, A]]
	[[Category: Kagan, O]]		[[Category: Kagan, O]]

OCA at 09:50, 4 January 2015

OCA — Sun, 04 Jan 2015 09:50:38 GMT

←Older revision		Revision as of 09:50, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3s8y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Oleispira_antarctica Oleispira antarctica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S8Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3S8Y FirstGlance]. <br>		<table><tr><td colspan='2'>[[3s8y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Oleispira_antarctica Oleispira antarctica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S8Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3S8Y FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i6y\|3i6y]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i6y\|3i6y]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">olei01171 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=188908 Oleispira antarctica])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">olei01171 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=188908 Oleispira antarctica])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arylesterase Arylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.2 3.1.1.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arylesterase Arylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.2 3.1.1.2] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s8y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3s8y RCSB], [http://www.ebi.ac.uk/pdbsum/3s8y PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s8y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3s8y RCSB], [http://www.ebi.ac.uk/pdbsum/3s8y PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 15:		Line 15:
	Structure and activity of the cold-active and anion-activated carboxyl esterase OLEI01171 from the oil-degrading marine bacterium Oleispira antarctica.,Lemak S, Tchigvintsev A, Petit P, Flick R, Singer AU, Brown G, Evdokimova E, Egorova O, Gonzalez CF, Chernikova TN, Yakimov MM, Kube M, Reinhardt R, Golyshin PN, Savchenko A, Yakunin AF Biochem J. 2012 Jul 15;445(2):193-203. PMID:22519667<ref>PMID:22519667</ref>		Structure and activity of the cold-active and anion-activated carboxyl esterase OLEI01171 from the oil-degrading marine bacterium Oleispira antarctica.,Lemak S, Tchigvintsev A, Petit P, Flick R, Singer AU, Brown G, Evdokimova E, Egorova O, Gonzalez CF, Chernikova TN, Yakimov MM, Kube M, Reinhardt R, Golyshin PN, Savchenko A, Yakunin AF Biochem J. 2012 Jul 15;445(2):193-203. PMID:22519667<ref>PMID:22519667</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 23:		Line 23:
	[[Category: Arylesterase]]		[[Category: Arylesterase]]
	[[Category: Oleispira antarctica]]		[[Category: Oleispira antarctica]]
-	[[Category: Dong, A.]]	+	[[Category: Dong, A]]
-	[[Category: Kagan, O.]]	+	[[Category: Kagan, O]]
-	[[Category: Petit, P.]]	+	[[Category: Petit, P]]
-	[[Category: Savchenko, A.]]	+	[[Category: Savchenko, A]]
-	[[Category: Yakunin, A F.]]	+	[[Category: Yakunin, A F]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Rossmann fold]]		[[Category: Rossmann fold]]

OCA at 05:21, 5 June 2014

OCA — Thu, 05 Jun 2014 05:21:16 GMT

←Older revision		Revision as of 05:21, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3s8y.~~png~~\|~~left~~\|~~200px~~]]	+	==Bromide soaked structure of an esterase from the oil-degrading bacterium Oleispira antarctica==
		+	<StructureSection load='3s8y' size='340' side='right' caption='[[3s8y]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3s8y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Oleispira_antarctica Oleispira antarctica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S8Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3S8Y FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3i6y\|3i6y]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">olei01171 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=188908 Oleispira antarctica])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Arylesterase Arylesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.2 3.1.1.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s8y OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3s8y RCSB], [http://www.ebi.ac.uk/pdbsum/3s8y PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The uncharacterized alpha/beta-hydrolase protein OLEI01171 from the psychrophilic marine bacterium Oleispira antarctica belongs to the PF00756 family of putative esterases, which also includes human esterase D. In the present paper we show that purified recombinant OLEI01171 exhibits high esterase activity against the model esterase substrate alpha-naphthyl acetate at 5-30 degrees C with maximal activity at 15-20 degrees C. The esterase activity of OLEI01171 was stimulated 3-8-fold by the addition of chloride or several other anions (0.1-1.0 M). Compared with mesophilic PF00756 esterases, OLEI01171 exhibited a lower overall protein thermostability. Two crystal structures of OLEI01171 were solved at 1.75 and 2.1 A resolution and revealed a classical serine hydrolase catalytic triad and the presence of a chloride or bromide ion bound in the active site close to the catalytic Ser148. Both anions were found to co-ordinate a potential catalytic water molecule located in the vicinity of the catalytic triad His257. The results of the present study suggest that the bound anion perhaps contributes to the polarization of the catalytic water molecule and increases the rate of the hydrolysis of an acyl-enzyme intermediate. Alanine replacement mutagenesis of OLEI01171 identified ten amino acid residues important for esterase activity. The replacement of Asn225 by lysine had no significant effect on the activity or thermostability of OLEI01171, but resulted in a detectable increase of activity at 35-45 degrees C. The present study has provided insight into the molecular mechanisms of activity of a cold-active and anion-activated carboxyl esterase.

-	~~{{STRUCTURE_3s8y\| PDB=3s8y \| SCENE= }}~~	+	Structure and activity of the cold-active and anion-activated carboxyl esterase OLEI01171 from the oil-degrading marine bacterium Oleispira antarctica.,Lemak S, Tchigvintsev A, Petit P, Flick R, Singer AU, Brown G, Evdokimova E, Egorova O, Gonzalez CF, Chernikova TN, Yakimov MM, Kube M, Reinhardt R, Golyshin PN, Savchenko A, Yakunin AF Biochem J. 2012 Jul 15;445(2):193-203. PMID:22519667<ref>PMID:22519667</ref>

-	~~===Bromide soaked structure~~ of ~~an esterase from~~ the ~~oil-degrading bacterium Oleispira antarctica===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22519667}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3s8y]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Oleispira_antarctica Oleispira antarctica]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3S8Y OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022519667<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Arylesterase]]		[[Category: Arylesterase]]
	[[Category: Oleispira antarctica]]		[[Category: Oleispira antarctica]]

OCA at 04:34, 18 July 2012

OCA — Wed, 18 Jul 2012 04:34:24 GMT

←Older revision		Revision as of 04:34, 18 July 2012
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-	[[Image:3s8y.~~jpg~~\|left\|200px]]	+	[[Image:3s8y.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3s8y", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3s8y\| PDB=3s8y \| SCENE= }}		{{STRUCTURE_3s8y\| PDB=3s8y \| SCENE= }}

	===Bromide soaked structure of an esterase from the oil-degrading bacterium Oleispira antarctica===		===Bromide soaked structure of an esterase from the oil-degrading bacterium Oleispira antarctica===

		+	{{ABSTRACT_PUBMED_22519667}}

	==About this Structure==		==About this Structure==
	[[3s8y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Oleispira_antarctica Oleispira antarctica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S8Y OCA].		[[3s8y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Oleispira_antarctica Oleispira antarctica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S8Y OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022519667</ref><references group="xtra"/>
	[[Category: Arylesterase]]		[[Category: Arylesterase]]
	[[Category: Oleispira antarctica]]		[[Category: Oleispira antarctica]]

OCA at 06:40, 15 June 2011

OCA — Wed, 15 Jun 2011 06:40:18 GMT

←Older revision		Revision as of 06:40, 15 June 2011
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-	~~'''Unreleased structure'''~~	+	[[Image:3s8y.jpg\|left\|200px]]

-	The ~~entry 3s8y~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3s8y", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3s8y\| PDB=3s8y \| SCENE= }}

-	~~Authors: Petit, P., Dong, A., Kagan, O., Savchenko, A., Yakunin, A.F.~~	+	===Bromide soaked structure of an esterase from the oil-degrading bacterium Oleispira antarctica===

-	~~Description: Bromide soaked~~ structure ~~of an esterase~~ from ~~the oil~~-~~degrading bacterium~~ Oleispira antarctica	+
		+	==About this Structure==
		+	[[3s8y]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Oleispira_antarctica Oleispira antarctica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S8Y OCA].
		+	[[Category: Arylesterase]]
		+	[[Category: Oleispira antarctica]]
		+	[[Category: Dong, A.]]
		+	[[Category: Kagan, O.]]
		+	[[Category: Petit, P.]]
		+	[[Category: Savchenko, A.]]
		+	[[Category: Yakunin, A F.]]
		+	[[Category: Hydrolase]]
		+	[[Category: Rossmann fold]]

OCA: Protected "3s8y" [edit=sysop:move=sysop]

OCA — Wed, 08 Jun 2011 04:55:10 GMT

Protected "3s8y" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:55, 8 June 2011

OCA: New page: '''Unreleased structure''' The entry 3s8y is ON HOLD Authors: Petit, P., Dong, A., Kagan, O., Savchenko, A., Yakunin, A.F. Description: Bromide soaked structure of an esterase from the...

OCA — Wed, 08 Jun 2011 04:55:09 GMT

New page: '''Unreleased structure''' The entry 3s8y is ON HOLD Authors: Petit, P., Dong, A., Kagan, O., Savchenko, A., Yakunin, A.F. Description: Bromide soaked structure of an esterase from the...

New page

'''Unreleased structure'''

The entry 3s8y is ON HOLD

Authors: Petit, P., Dong, A., Kagan, O., Savchenko, A., Yakunin, A.F.

Description: Bromide soaked structure of an esterase from the oil-degrading bacterium Oleispira antarctica