3sds - Revision history

OCA at 09:49, 1 March 2024

OCA — Fri, 01 Mar 2024 09:49:42 GMT

←Older revision		Revision as of 09:49, 1 March 2024
Line 3:		Line 3:
	<StructureSection load='3sds' size='340' side='right'caption='[[3sds]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='3sds' size='340' side='right'caption='[[3sds]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3sds]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Cocim Cocim~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SDS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SDS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3sds]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Coccidioides_immitis_RS Coccidioides immitis RS]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SDS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SDS FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>CIMG_04084 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~246410 COCIM])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Ornithine_carbamoyltransferase Ornithine carbamoyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.3 2.1.3.3]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sds FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sds OCA], [https://pdbe.org/3sds PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sds RCSB], [https://www.ebi.ac.uk/pdbsum/3sds PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sds ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sds FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sds OCA], [https://pdbe.org/3sds PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sds RCSB], [https://www.ebi.ac.uk/pdbsum/3sds PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sds ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/OTC_COCIM OTC_COCIM]
-	Despite recent advances, the expression of heterologous proteins in Escherichia coli for crystallization remains a nontrivial challenge. The present study investigates the efficacy of maltose-binding protein (MBP) fusion as a general strategy for rescuing the expression of target proteins. From a group of sequence-verified clones with undetectable levels of protein expression in an E. coli T7 expression system, 95 clones representing 16 phylogenetically diverse organisms were selected for recloning into a chimeric expression vector with an N-terminal histidine-tagged MBP. PCR-amplified inserts were annealed into an identical ligation-independent cloning region in an MBP-fusion vector and were analyzed for expression and solubility by high-throughput nickel-affinity binding. This approach yielded detectable expression of 72% of the clones; soluble expression was visible in 62%. However, the solubility of most proteins was marginal to poor upon cleavage of the MBP tag. This study offers large-scale evidence that MBP can improve the soluble expression of previously non-expressing proteins from a variety of eukaryotic and prokaryotic organisms. While the behavior of the cleaved proteins was disappointing, further refinements in MBP tagging may permit the more widespread use of MBP-fusion proteins in crystallographic studies.	+
-		+
-	Expression of proteins in Escherichia coli as fusions with maltose-binding protein to rescue non-expressed targets in a high-throughput protein-expression and purification pipeline.,Hewitt SN, Choi R, Kelley A, Crowther GJ, Napuli AJ, Van Voorhis WC Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Sep 1;67(Pt, 9):~~1006-9. Epub 2011 Aug 13. PMID:21904041<ref>PMID:21904041<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3sds" style="background-color:#fffaf0;"><~~/~~div>~~	+

	==See Also==		==See Also==
	*[[Ornithine carbamoyltransferase 3D structures\|Ornithine carbamoyltransferase 3D structures]]		*[[Ornithine carbamoyltransferase 3D structures\|Ornithine carbamoyltransferase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Cocim~~]]	+	[[Category: Coccidioides immitis RS]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Ornithine carbamoyltransferase]]~~	+	[[Category: Abendroth J]]
-	[[Category: Abendroth, J]]	+	[[Category: Edwards TE]]
-	[[Category: Edwards~~, T E]]~~	+
-	~~[[Category: Structural genomic]]~~	+
-	~~[[Category: Amino acid biosynthesis]]~~	+
-	~~[[Category: Carbamoyl phosphate]]~~	+
-	~~[[Category: Coccidioidomycosis]]~~	+
-	~~[[Category: Dust-borne pathogen]]~~	+
-	~~[[Category: L-citrulline]]~~	+
-	~~[[Category: L-ornithine]]~~	+
-	~~[[Category: Mitochondrial]]~~	+
-	~~[[Category: Otcase]]~~	+
-	~~[[Category: Pathogenic fungus]]~~	+
-	~~[[Category: Ssgcid]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Valley fever~~]]	+

OCA at 07:52, 29 June 2022

OCA — Wed, 29 Jun 2022 07:52:36 GMT

←Older revision		Revision as of 07:52, 29 June 2022
Line 1:		Line 1:

	==Crystal structure of a mitochondrial ornithine carbamoyltransferase from Coccidioides immitis==		==Crystal structure of a mitochondrial ornithine carbamoyltransferase from Coccidioides immitis==
-	<StructureSection load='3sds' size='340' side='right' caption='[[3sds]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='3sds' size='340' side='right'caption='[[3sds]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3sds]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Cocim Cocim]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SDS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3SDS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3sds]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Cocim Cocim]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SDS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SDS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CIMG_04084 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=246410 COCIM])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CIMG_04084 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=246410 COCIM])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Ornithine_carbamoyltransferase Ornithine carbamoyltransferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.3 2.1.3.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Ornithine_carbamoyltransferase Ornithine carbamoyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.3 2.1.3.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3sds FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sds OCA], [~~http~~://pdbe.org/3sds PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3sds RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3sds PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3sds ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sds FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sds OCA], [https://pdbe.org/3sds PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sds RCSB], [https://www.ebi.ac.uk/pdbsum/3sds PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sds ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 20:

	==See Also==		==See Also==
-	*[[Ornithine carbamoyltransferase\|Ornithine carbamoyltransferase]]	+	*[[Ornithine carbamoyltransferase 3D structures\|Ornithine carbamoyltransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 26:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Cocim]]		[[Category: Cocim]]
		+	[[Category: Large Structures]]
	[[Category: Ornithine carbamoyltransferase]]		[[Category: Ornithine carbamoyltransferase]]
	[[Category: Abendroth, J]]		[[Category: Abendroth, J]]

OCA at 21:21, 11 August 2016

OCA — Thu, 11 Aug 2016 21:21:46 GMT

←Older revision		Revision as of 21:21, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of a mitochondrial ornithine carbamoyltransferase from Coccidioides immitis==		==Crystal structure of a mitochondrial ornithine carbamoyltransferase from Coccidioides immitis==
	<StructureSection load='3sds' size='340' side='right' caption='[[3sds]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='3sds' size='340' side='right' caption='[[3sds]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3sds]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Coccidioides_immitis Coccidioides immitis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SDS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SDS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3sds]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Cocim Cocim]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SDS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SDS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CIMG_04084 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~5501 Coccidioides immitis~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CIMG_04084 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=246410 COCIM])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ornithine_carbamoyltransferase Ornithine carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.3 2.1.3.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ornithine_carbamoyltransferase Ornithine carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.3 2.1.3.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sds FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sds OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3sds RCSB], [http://www.ebi.ac.uk/pdbsum/3sds PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sds FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sds OCA], [http://pdbe.org/3sds PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3sds RCSB], [http://www.ebi.ac.uk/pdbsum/3sds PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3sds ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3sds" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 23:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Coccidioides immitis~~]]	+	[[Category: Cocim]]
	[[Category: Ornithine carbamoyltransferase]]		[[Category: Ornithine carbamoyltransferase]]
	[[Category: Abendroth, J]]		[[Category: Abendroth, J]]

OCA at 11:05, 4 January 2015

OCA — Sun, 04 Jan 2015 11:05:23 GMT

←Older revision		Revision as of 11:05, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3sds]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Coccidioides_immitis Coccidioides immitis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SDS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SDS FirstGlance]. <br>		<table><tr><td colspan='2'>[[3sds]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Coccidioides_immitis Coccidioides immitis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SDS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SDS FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CIMG_04084 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5501 Coccidioides immitis])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CIMG_04084 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5501 Coccidioides immitis])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ornithine_carbamoyltransferase Ornithine carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.3 2.1.3.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ornithine_carbamoyltransferase Ornithine carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.3 2.1.3.3] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sds FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sds OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3sds RCSB], [http://www.ebi.ac.uk/pdbsum/3sds PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sds FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sds OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3sds RCSB], [http://www.ebi.ac.uk/pdbsum/3sds PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 14:
	Expression of proteins in Escherichia coli as fusions with maltose-binding protein to rescue non-expressed targets in a high-throughput protein-expression and purification pipeline.,Hewitt SN, Choi R, Kelley A, Crowther GJ, Napuli AJ, Van Voorhis WC Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Sep 1;67(Pt, 9):1006-9. Epub 2011 Aug 13. PMID:21904041<ref>PMID:21904041</ref>		Expression of proteins in Escherichia coli as fusions with maltose-binding protein to rescue non-expressed targets in a high-throughput protein-expression and purification pipeline.,Hewitt SN, Choi R, Kelley A, Crowther GJ, Napuli AJ, Van Voorhis WC Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Sep 1;67(Pt, 9):1006-9. Epub 2011 Aug 13. PMID:21904041<ref>PMID:21904041</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>

Line 25:		Line 25:
	[[Category: Coccidioides immitis]]		[[Category: Coccidioides immitis]]
	[[Category: Ornithine carbamoyltransferase]]		[[Category: Ornithine carbamoyltransferase]]
-	[[Category: Abendroth, J.]]	+	[[Category: Abendroth, J]]
-	[[Category: Edwards, T E.]]	+	[[Category: Edwards, T E]]
-	[[Category: ~~SSGCID, Seattle~~ Structural ~~Genomics Center for Infectious Disease.~~]]	+	[[Category: Structural genomic]]
	[[Category: Amino acid biosynthesis]]		[[Category: Amino acid biosynthesis]]
	[[Category: Carbamoyl phosphate]]		[[Category: Carbamoyl phosphate]]
Line 37:		Line 37:
	[[Category: Otcase]]		[[Category: Otcase]]
	[[Category: Pathogenic fungus]]		[[Category: Pathogenic fungus]]
-	[[Category: Seattle structural genomics center for infectious disease]]
	[[Category: Ssgcid]]		[[Category: Ssgcid]]
-	[[Category: Structural genomic]]
	[[Category: Transferase]]		[[Category: Transferase]]
	[[Category: Valley fever]]		[[Category: Valley fever]]

OCA at 05:29, 5 June 2014

OCA — Thu, 05 Jun 2014 05:29:21 GMT

←Older revision		Revision as of 05:29, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3sds.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of a mitochondrial ornithine carbamoyltransferase from Coccidioides immitis==
		+	<StructureSection load='3sds' size='340' side='right' caption='[[3sds]], [[Resolution\|resolution]] 2.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3sds]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Coccidioides_immitis Coccidioides immitis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SDS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SDS FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CIMG_04084 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5501 Coccidioides immitis])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ornithine_carbamoyltransferase Ornithine carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.3 2.1.3.3] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sds FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sds OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3sds RCSB], [http://www.ebi.ac.uk/pdbsum/3sds PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Despite recent advances, the expression of heterologous proteins in Escherichia coli for crystallization remains a nontrivial challenge. The present study investigates the efficacy of maltose-binding protein (MBP) fusion as a general strategy for rescuing the expression of target proteins. From a group of sequence-verified clones with undetectable levels of protein expression in an E. coli T7 expression system, 95 clones representing 16 phylogenetically diverse organisms were selected for recloning into a chimeric expression vector with an N-terminal histidine-tagged MBP. PCR-amplified inserts were annealed into an identical ligation-independent cloning region in an MBP-fusion vector and were analyzed for expression and solubility by high-throughput nickel-affinity binding. This approach yielded detectable expression of 72% of the clones; soluble expression was visible in 62%. However, the solubility of most proteins was marginal to poor upon cleavage of the MBP tag. This study offers large-scale evidence that MBP can improve the soluble expression of previously non-expressing proteins from a variety of eukaryotic and prokaryotic organisms. While the behavior of the cleaved proteins was disappointing, further refinements in MBP tagging may permit the more widespread use of MBP-fusion proteins in crystallographic studies.

-	<!--	+	Expression of proteins in Escherichia coli as fusions with maltose-binding protein to rescue non-expressed targets in a high-throughput protein-expression and purification pipeline.,Hewitt SN, Choi R, Kelley A, Crowther GJ, Napuli AJ, Van Voorhis WC Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Sep 1;67(Pt, 9):1006-9. Epub 2011 Aug 13. PMID:21904041<ref>PMID:21904041</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3sds"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded)~~,	+
-	~~or leave the SCENE parameter empty for the default display.~~	+
-	-->	+
-	~~{{STRUCTURE_3sds\| PDB=3sds \| SCENE= }}~~	+

-	~~===Crystal structure of~~ a ~~mitochondrial ornithine carbamoyltransferase from Coccidioides immitis===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-		+	==See Also==
-	~~<!--~~	+	*[[Ornithine carbamoyltransferase\|Ornithine carbamoyltransferase]]
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_21904041}}, adds the Publication Abstract to the page~~	+	== References ==
-	~~(as it appears on PubMed at http://www.pubmed.gov), where 21904041 is the PubMed ID number.~~	+	<references/>
-	~~-->~~	+	__TOC__
-	~~{{ABSTRACT_PUBMED_21904041}}~~	+	</StructureSection>
-		+
-	==~~About this Structure~~==	+
-	[[~~3sds~~]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Coccidioides_immitis Coccidioides immitis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SDS OCA].	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:021904041<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Coccidioides immitis]]		[[Category: Coccidioides immitis]]
	[[Category: Ornithine carbamoyltransferase]]		[[Category: Ornithine carbamoyltransferase]]

OCA at 05:10, 21 September 2011

OCA — Wed, 21 Sep 2011 05:10:49 GMT

←Older revision		Revision as of 05:10, 21 September 2011
Line 1:		Line 1:
-	[[Image:3sds.~~jpg~~\|left\|200px]]	+	[[Image:3sds.png\|left\|200px]]

	<!--		<!--
Line 11:		Line 11:
	===Crystal structure of a mitochondrial ornithine carbamoyltransferase from Coccidioides immitis===		===Crystal structure of a mitochondrial ornithine carbamoyltransferase from Coccidioides immitis===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21904041}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21904041 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21904041}}

	==About this Structure==		==About this Structure==
	[[3sds]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Coccidioides_immitis Coccidioides immitis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SDS OCA].		[[3sds]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Coccidioides_immitis Coccidioides immitis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SDS OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021904041</ref><references group="xtra"/>
	[[Category: Coccidioides immitis]]		[[Category: Coccidioides immitis]]
	[[Category: Ornithine carbamoyltransferase]]		[[Category: Ornithine carbamoyltransferase]]
		+	[[Category: Abendroth, J.]]
		+	[[Category: Edwards, T E.]]
	[[Category: SSGCID, Seattle Structural Genomics Center for Infectious Disease.]]		[[Category: SSGCID, Seattle Structural Genomics Center for Infectious Disease.]]
	[[Category: Amino acid biosynthesis]]		[[Category: Amino acid biosynthesis]]

OCA at 16:56, 22 June 2011

OCA — Wed, 22 Jun 2011 16:56:26 GMT

←Older revision		Revision as of 16:56, 22 June 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3sds.jpg\|left\|200px]]

-	The ~~entry 3sds~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3sds", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3sds\| PDB=3sds \| SCENE= }}

-	~~Authors: Seattle Structural Genomics Center for Infectious Disease (SSGCID)~~	+	===Crystal structure of a mitochondrial ornithine carbamoyltransferase from Coccidioides immitis===

-	~~Description: Crystal structure of~~ a ~~mitochondrial ornithine carbamoyltransferase~~ from Coccidioides immitis	+
		+	==About this Structure==
		+	[[3sds]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Coccidioides_immitis Coccidioides immitis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SDS OCA].
		+	[[Category: Coccidioides immitis]]
		+	[[Category: Ornithine carbamoyltransferase]]
		+	[[Category: SSGCID, Seattle Structural Genomics Center for Infectious Disease.]]
		+	[[Category: Amino acid biosynthesis]]
		+	[[Category: Carbamoyl phosphate]]
		+	[[Category: Coccidioidomycosis]]
		+	[[Category: Dust-borne pathogen]]
		+	[[Category: L-citrulline]]
		+	[[Category: L-ornithine]]
		+	[[Category: Mitochondrial]]
		+	[[Category: Otcase]]
		+	[[Category: Pathogenic fungus]]
		+	[[Category: Seattle structural genomics center for infectious disease]]
		+	[[Category: Ssgcid]]
		+	[[Category: Structural genomic]]
		+	[[Category: Transferase]]
		+	[[Category: Valley fever]]

OCA: Protected "3sds" [edit=sysop:move=sysop]

OCA — Wed, 15 Jun 2011 05:59:52 GMT

Protected "3sds" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:59, 15 June 2011

OCA: New page: '''Unreleased structure''' The entry 3sds is ON HOLD Authors: Seattle Structural Genomics Center for Infectious Disease (SSGCID) Description: Crystal structure of a mitochondrial ornit...

OCA — Wed, 15 Jun 2011 05:59:52 GMT

New page: '''Unreleased structure''' The entry 3sds is ON HOLD Authors: Seattle Structural Genomics Center for Infectious Disease (SSGCID) Description: Crystal structure of a mitochondrial ornit...

New page

'''Unreleased structure'''

The entry 3sds is ON HOLD

Authors: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Description: Crystal structure of a mitochondrial ornithine carbamoyltransferase from Coccidioides immitis