3sfg - Revision history

OCA at 07:54, 29 June 2022

OCA — Wed, 29 Jun 2022 07:54:17 GMT

←Older revision		Revision as of 07:54, 29 June 2022
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	==crystal structure of murine norovirus RNA dependent RNA polymerase in complex with 2thiouridine(2TU)==		==crystal structure of murine norovirus RNA dependent RNA polymerase in complex with 2thiouridine(2TU)==
-	<StructureSection load='3sfg' size='340' side='right' caption='[[3sfg]], [[Resolution\|resolution]] 2.21Å' scene=''>	+	<StructureSection load='3sfg' size='340' side='right'caption='[[3sfg]], [[Resolution\|resolution]] 2.21Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3sfg]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Murine_norovirus_1 Murine norovirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SFG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3SFG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3sfg]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Murine_norovirus_1 Murine norovirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SFG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SFG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2TU:1-(BETA-D-RIBOFURANOSYL)-2-THIOXO-2,3-DIHYDROPYRIMIDIN-4(1H)-ONE'>2TU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2TU:1-(BETA-D-RIBOFURANOSYL)-2-THIOXO-2,3-DIHYDROPYRIMIDIN-4(1H)-ONE'>2TU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3nah\|3nah]], [[3nai\|3nai]], [[3qid\|3qid]], [[3sfu\|3sfu]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3nah\|3nah]], [[3nai\|3nai]], [[3qid\|3qid]], [[3sfu\|3sfu]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RdRp ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=223997 Murine norovirus 1])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RdRp ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=223997 Murine norovirus 1])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3sfg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sfg OCA], [~~http~~://pdbe.org/3sfg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3sfg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3sfg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3sfg ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sfg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sfg OCA], [https://pdbe.org/3sfg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sfg RCSB], [https://www.ebi.ac.uk/pdbsum/3sfg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sfg ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 20:

	==See Also==		==See Also==
-	*[[RNA polymerase\|RNA polymerase]]	+	*[[RNA polymerase 3D structures\|RNA polymerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Murine norovirus 1]]		[[Category: Murine norovirus 1]]
	[[Category: Alam, I]]		[[Category: Alam, I]]

OCA at 11:20, 10 December 2016

OCA — Sat, 10 Dec 2016 11:20:42 GMT

←Older revision		Revision as of 11:20, 10 December 2016
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		+
	==crystal structure of murine norovirus RNA dependent RNA polymerase in complex with 2thiouridine(2TU)==		==crystal structure of murine norovirus RNA dependent RNA polymerase in complex with 2thiouridine(2TU)==
	<StructureSection load='3sfg' size='340' side='right' caption='[[3sfg]], [[Resolution\|resolution]] 2.21Å' scene=''>		<StructureSection load='3sfg' size='340' side='right' caption='[[3sfg]], [[Resolution\|resolution]] 2.21Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3nah\|3nah]], [[3nai\|3nai]], [[3qid\|3qid]], [[3sfu\|3sfu]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3nah\|3nah]], [[3nai\|3nai]], [[3qid\|3qid]], [[3sfu\|3sfu]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RdRp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=223997 Murine norovirus 1])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RdRp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=223997 Murine norovirus 1])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sfg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sfg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3sfg RCSB], [http://www.ebi.ac.uk/pdbsum/3sfg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sfg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sfg OCA], [http://pdbe.org/3sfg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3sfg RCSB], [http://www.ebi.ac.uk/pdbsum/3sfg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3sfg ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3sfg" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 10:54, 9 December 2014

OCA — Tue, 09 Dec 2014 10:54:01 GMT

←Older revision		Revision as of 10:54, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3sfg.~~png~~\|~~left~~\|~~200px~~]]	+	==crystal structure of murine norovirus RNA dependent RNA polymerase in complex with 2thiouridine(2TU)==
		+	<StructureSection load='3sfg' size='340' side='right' caption='[[3sfg]], [[Resolution\|resolution]] 2.21Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3sfg]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Murine_norovirus_1 Murine norovirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SFG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SFG FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2TU:1-(BETA-D-RIBOFURANOSYL)-2-THIOXO-2,3-DIHYDROPYRIMIDIN-4(1H)-ONE'>2TU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3nah\|3nah]], [[3nai\|3nai]], [[3qid\|3qid]], [[3sfu\|3sfu]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RdRp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=223997 Murine norovirus 1])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sfg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sfg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3sfg RCSB], [http://www.ebi.ac.uk/pdbsum/3sfg PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Murine norovirus-1 (MNV-1) shares many features with human norovirus (HuNoV) and both are classified within the norovirus genus of Caliciviridae family. MNV-1 is used as the surrogate for HuNoV research since it is the only form that can be grown in cell culture. HuNoV and MNV-1 RNA dependent RNA polymerase (RdRp) proteins with the sequence identity of 59% show essentially identical conformations. Here we report the first structural evidence of 2-thiouridine (2TU) or ribavirin binding to MNV-1 RdRp, based on the crystal structures determined at 2.2A and 2.5A resolutions, respectively. Cellular and biochemical studies revealed stronger inhibitory effect of 2TU on the replication of MNV-1 in RAW 264.7 cells, compared to that of ribavirin. Our complex structures highlight the key interactions involved in recognition of the nucleoside analogs which block the active site of the viral RNA polymerase.

-	~~{{STRUCTURE_3sfg\| PDB=3sfg \| SCENE= }}~~	+	Crystal structures of murine norovirus-1 RNA-dependent RNA polymerase in complex with 2-thiouridine or ribavirin.,Alam I, Lee JH, Cho KJ, Han KR, Yang JM, Chung MS, Kim KH Virology. 2012 May 10;426(2):143-51. Epub 2012 Feb 16. PMID:22341781<ref>PMID:22341781</ref>

-	~~===crystal structure~~ of ~~murine norovirus RNA dependent RNA polymerase in complex with 2thiouridine(2TU)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22341781}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3sfg]] is a 3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Murine_norovirus_1 Murine norovirus 1]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3SFG OCA].~~	+

	==See Also==		==See Also==
	*[[RNA polymerase\|RNA polymerase]]		*[[RNA polymerase\|RNA polymerase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:022341781<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Murine norovirus 1]]		[[Category: Murine norovirus 1]]
-	[[Category: Alam, I.]]	+	[[Category: Alam, I]]
-	[[Category: Kim, K H.]]	+	[[Category: Kim, K H]]
	[[Category: Polymerase]]		[[Category: Polymerase]]
	[[Category: Right hand conformation]]		[[Category: Right hand conformation]]
	[[Category: Rna binding]]		[[Category: Rna binding]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 19:47, 20 October 2012

OCA — Sat, 20 Oct 2012 19:47:37 GMT

←Older revision		Revision as of 19:47, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[3sfg]] is a 3 chain structure ~~of [[RNA polymerase]]~~ with sequence from [http://en.wikipedia.org/wiki/Murine_norovirus_1 Murine norovirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SFG OCA].	+	[[3sfg]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Murine_norovirus_1 Murine norovirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SFG OCA].

	==See Also==		==See Also==

OCA at 08:30, 27 July 2012

OCA — Fri, 27 Jul 2012 08:30:40 GMT

←Older revision		Revision as of 08:30, 27 July 2012
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-	[[Image:3sfg.~~jpg~~\|left\|200px]]	+	[[Image:3sfg.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3sfg", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3sfg\| PDB=3sfg \| SCENE= }}		{{STRUCTURE_3sfg\| PDB=3sfg \| SCENE= }}

	===crystal structure of murine norovirus RNA dependent RNA polymerase in complex with 2thiouridine(2TU)===		===crystal structure of murine norovirus RNA dependent RNA polymerase in complex with 2thiouridine(2TU)===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_22341781}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 22341781 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_22341781}}		{{ABSTRACT_PUBMED_22341781}}

	==About this Structure==		==About this Structure==
-	[[3sfg]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Murine_norovirus_1 Murine norovirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SFG OCA].	+	[[3sfg]] is a 3 chain structure of [[RNA polymerase]] with sequence from [http://en.wikipedia.org/wiki/Murine_norovirus_1 Murine norovirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SFG OCA].
		+
		+	==See Also==
		+	*[[RNA polymerase\|RNA polymerase]]

	==Reference==		==Reference==

OCA at 09:13, 9 May 2012

OCA — Wed, 09 May 2012 09:13:05 GMT

←Older revision		Revision as of 09:13, 9 May 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3sfg.jpg\|left\|200px]]

-	The ~~entry 3sfg~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3sfg", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3sfg\| PDB=3sfg \| SCENE= }}

-	~~Authors: Kim, K.H., Intekhab, A.~~	+	===crystal structure of murine norovirus RNA dependent RNA polymerase in complex with 2thiouridine(2TU)===

-	~~Description~~: ~~crystal~~ structure ~~of murine norovirus RNA dependent RNA polymerase in complex~~ with ~~2thiouridine(2TU)~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22341781}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22341781 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22341781}}
		+
		+	==About this Structure==
		+	[[3sfg]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Murine_norovirus_1 Murine norovirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SFG OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022341781</ref><references group="xtra"/>
		+	[[Category: Murine norovirus 1]]
		+	[[Category: Alam, I.]]
		+	[[Category: Kim, K H.]]
		+	[[Category: Polymerase]]
		+	[[Category: Right hand conformation]]
		+	[[Category: Rna binding]]
		+	[[Category: Transferase]]

OCA at 06:11, 20 July 2011

OCA — Wed, 20 Jul 2011 06:11:25 GMT

←Older revision		Revision as of 06:11, 20 July 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3sfg is ON HOLD	+	The entry 3sfg is ON HOLD until Paper Publication

	Authors: Kim, K.H., Intekhab, A.		Authors: Kim, K.H., Intekhab, A.

	Description: crystal structure of murine norovirus RNA dependent RNA polymerase in complex with 2thiouridine(2TU)		Description: crystal structure of murine norovirus RNA dependent RNA polymerase in complex with 2thiouridine(2TU)

OCA at 18:25, 6 July 2011

OCA — Wed, 06 Jul 2011 18:25:27 GMT

←Older revision		Revision as of 18:25, 6 July 2011
Line 3:		Line 3:
	The entry 3sfg is ON HOLD		The entry 3sfg is ON HOLD

-	Authors: Kim ~~Kyung Hyun~~, ~~Alam~~ Intekhab	+	Authors: Kim, K.H., Intekhab, A.

	Description: crystal structure of murine norovirus RNA dependent RNA polymerase in complex with 2thiouridine(2TU)		Description: crystal structure of murine norovirus RNA dependent RNA polymerase in complex with 2thiouridine(2TU)

OCA: Protected "3sfg" [edit=sysop:move=sysop]

OCA — Wed, 22 Jun 2011 16:22:45 GMT

Protected "3sfg" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:22, 22 June 2011

OCA: New page: '''Unreleased structure''' The entry 3sfg is ON HOLD Authors: Kim Kyung Hyun, Alam Intekhab Description: crystal structure of murine norovirus RNA dependent RNA polymerase in complex w...

OCA — Wed, 22 Jun 2011 16:22:44 GMT

New page: '''Unreleased structure''' The entry 3sfg is ON HOLD Authors: Kim Kyung Hyun, Alam Intekhab Description: crystal structure of murine norovirus RNA dependent RNA polymerase in complex w...

New page

'''Unreleased structure'''

The entry 3sfg is ON HOLD

Authors: Kim Kyung Hyun, Alam Intekhab

Description: crystal structure of murine norovirus RNA dependent RNA polymerase in complex with 2thiouridine(2TU)