3smc - Revision history

OCA at 13:01, 14 March 2024

OCA — Thu, 14 Mar 2024 13:01:16 GMT

←Older revision		Revision as of 13:01, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3smc' size='340' side='right'caption='[[3smc]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3smc' size='340' side='right'caption='[[3smc]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3smc]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SMC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SMC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3smc]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SMC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SMC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=LE3:N-{4-[(R)-METHYLSULFINYL]BUTYL}THIOFORMAMIDE'>LE3</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3smb\|3smb]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=LE3:N-{4-[(R)-METHYLSULFINYL]BUTYL}THIOFORMAMIDE'>LE3</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GLIF, MIF, MMIF ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3smc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3smc OCA], [https://pdbe.org/3smc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3smc RCSB], [https://www.ebi.ac.uk/pdbsum/3smc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3smc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3smc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3smc OCA], [https://pdbe.org/3smc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3smc RCSB], [https://www.ebi.ac.uk/pdbsum/3smc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3smc ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[[https://www.uniprot.org/uniprot/MIF_HUMAN MIF_HUMAN]] Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:[https://omim.org/entry/604302 604302]]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.	+	[https://www.uniprot.org/uniprot/MIF_HUMAN MIF_HUMAN] Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:[https://omim.org/entry/604302 604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/MIF_HUMAN MIF_HUMAN]] Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.<ref>PMID:15908412</ref> <ref>PMID:17443469</ref>	+	[https://www.uniprot.org/uniprot/MIF_HUMAN MIF_HUMAN] Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.<ref>PMID:15908412</ref> <ref>PMID:17443469</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Regulation of cellular processes by dietary nutrients is known to affect the likelihood of cancer development. One class of cancer-preventive nutrients, isothiocyanates (ITCs), derived from the consumption of cruciferous vegetables, is known to have various effects on cellular biochemistry. One target of ITCs is macrophage migration inhibitory factor (MIF), a widely expressed protein with known inflammatory, pro-tumorigenic, pro-angiogenic, and anti-apoptotic properties. MIF is covalently inhibited by a variety of ITCs, which in part may explain how they exert their cancer-preventive effects. We report the crystallographic structures of human MIF bound to phenethylisothiocyanate and to l-sulforaphane (dietary isothiocyanates derived from watercress and broccoli, respectively) and correlate structural features of these two isothiocyanates with their second-order rate constants for MIF inactivation. We also characterize changes in the MIF structure using nuclear magnetic resonance heteronuclear single-quantum coherence spectra of these complexes and observe many changes at the subunit interface. While a number of chemical shifts do not change, many of those that change do not have features similar in magnitude or direction for the two isothiocyanates. The difference in the binding modes of these two ITCs provides a means of using structure-activity relationships to reveal insights into MIF biological interactions. The results of this study provide a framework for the development of therapeutics that target MIF.	+
-		+
-	Structural Interactions Dictate the Kinetics of Macrophage Migration Inhibitory Factor Inhibition by Different Cancer-Preventive Isothiocyanates.,Crichlow GV, Fan C, Keeler C, Hodsdon M, Lolis EJ Biochemistry. 2012 Sep 11. PMID:22931430<ref>PMID:22931430</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3smc" style="background-color:#fffaf0;"></div~~>	+

	==See Also==		==See Also==
Line 29:		Line 19:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Crichlow~~, G V~~]]	+	[[Category: Crichlow GV]]
-	[[Category: Lolis~~, E J]]~~	+	[[Category: Lolis EJ]]
-	~~[[Category: Cytokine]]~~	+
-	~~[[Category: Isomerase-isomerase inhibitor complex]]~~	+
-	~~[[Category: Receptor binding]]~~	+
-	~~[[Category: Secreted~~]]	+

OCA at 08:01, 29 June 2022

OCA — Wed, 29 Jun 2022 08:01:52 GMT

←Older revision		Revision as of 08:01, 29 June 2022
Line 1:		Line 1:

	==Macrophage Migration Inhibitory Factor (MIF) with Covalently Bound L-sulforaphane==		==Macrophage Migration Inhibitory Factor (MIF) with Covalently Bound L-sulforaphane==
-	<StructureSection load='3smc' size='340' side='right' caption='[[3smc]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='3smc' size='340' side='right'caption='[[3smc]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3smc]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SMC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3SMC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3smc]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SMC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SMC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=LE3:N-{4-[(R)-METHYLSULFINYL]BUTYL}THIOFORMAMIDE'>LE3</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=LE3:N-{4-[(R)-METHYLSULFINYL]BUTYL}THIOFORMAMIDE'>LE3</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3smb\|3smb]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3smb\|3smb]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GLIF, MIF, MMIF ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GLIF, MIF, MMIF ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3smc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3smc OCA], [~~http~~://pdbe.org/3smc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3smc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3smc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3smc ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3smc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3smc OCA], [https://pdbe.org/3smc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3smc RCSB], [https://www.ebi.ac.uk/pdbsum/3smc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3smc ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[[~~http~~://www.uniprot.org/uniprot/MIF_HUMAN MIF_HUMAN]] Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:[~~http~~://omim.org/entry/604302 604302]]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.	+	[[https://www.uniprot.org/uniprot/MIF_HUMAN MIF_HUMAN]] Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:[https://omim.org/entry/604302 604302]]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/MIF_HUMAN MIF_HUMAN]] Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.<ref>PMID:15908412</ref> <ref>PMID:17443469</ref>	+	[[https://www.uniprot.org/uniprot/MIF_HUMAN MIF_HUMAN]] Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.<ref>PMID:15908412</ref> <ref>PMID:17443469</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:

	==See Also==		==See Also==
-	*[[Macrophage inhibitory factor\|Macrophage inhibitory factor]]	+	*[[Macrophage inhibitory factor 3D structures\|Macrophage inhibitory factor 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 30:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Crichlow, G V]]		[[Category: Crichlow, G V]]
	[[Category: Lolis, E J]]		[[Category: Lolis, E J]]

OCA at 19:56, 5 August 2016

OCA — Fri, 05 Aug 2016 19:56:43 GMT

←Older revision		Revision as of 19:56, 5 August 2016
Line 1:		Line 1:
		+
	==Macrophage Migration Inhibitory Factor (MIF) with Covalently Bound L-sulforaphane==		==Macrophage Migration Inhibitory Factor (MIF) with Covalently Bound L-sulforaphane==
	<StructureSection load='3smc' size='340' side='right' caption='[[3smc]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='3smc' size='340' side='right' caption='[[3smc]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3smc]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SMC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SMC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3smc]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SMC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SMC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=LE3:N-{4-[(R)-METHYLSULFINYL]BUTYL}THIOFORMAMIDE'>LE3</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=LE3:N-{4-[(R)-METHYLSULFINYL]BUTYL}THIOFORMAMIDE'>LE3</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3smb\|3smb]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3smb\|3smb]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GLIF, MIF, MMIF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GLIF, MIF, MMIF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3smc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3smc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3smc RCSB], [http://www.ebi.ac.uk/pdbsum/3smc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3smc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3smc OCA], [http://pdbe.org/3smc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3smc RCSB], [http://www.ebi.ac.uk/pdbsum/3smc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3smc ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 20:		Line 21:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3smc" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 27:		Line 29:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Crichlow, G V]]		[[Category: Crichlow, G V]]
	[[Category: Lolis, E J]]		[[Category: Lolis, E J]]

OCA at 06:52, 21 December 2014

OCA — Sun, 21 Dec 2014 06:52:03 GMT

←Older revision		Revision as of 06:52, 21 December 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_3smc\| PDB=3smc \| SCENE= }}~~	+	==Macrophage Migration Inhibitory Factor (MIF) with Covalently Bound L-sulforaphane==
-	===Macrophage Migration Inhibitory Factor (MIF) with Covalently Bound L-sulforaphane===	+	<StructureSection load='3smc' size='340' side='right' caption='[[3smc]], [[Resolution\|resolution]] 1.80Å' scene=''>
-	{~~{ABSTRACT_PUBMED_22931430}~~}	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[3smc]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SMC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SMC FirstGlance]. <br>
-	==Disease==	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=LE3:N-{4-[(R)-METHYLSULFINYL]BUTYL}THIOFORMAMIDE'>LE3</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3smb\|3smb]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GLIF, MIF, MMIF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3smc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3smc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3smc RCSB], [http://www.ebi.ac.uk/pdbsum/3smc PDBsum]</span></td></tr>
		+	</table>
		+	== Disease ==
	[[http://www.uniprot.org/uniprot/MIF_HUMAN MIF_HUMAN]] Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:[http://omim.org/entry/604302 604302]]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.		[[http://www.uniprot.org/uniprot/MIF_HUMAN MIF_HUMAN]] Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:[http://omim.org/entry/604302 604302]]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MIF_HUMAN MIF_HUMAN]] Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.<ref>PMID:15908412</ref> <ref>PMID:17443469</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Regulation of cellular processes by dietary nutrients is known to affect the likelihood of cancer development. One class of cancer-preventive nutrients, isothiocyanates (ITCs), derived from the consumption of cruciferous vegetables, is known to have various effects on cellular biochemistry. One target of ITCs is macrophage migration inhibitory factor (MIF), a widely expressed protein with known inflammatory, pro-tumorigenic, pro-angiogenic, and anti-apoptotic properties. MIF is covalently inhibited by a variety of ITCs, which in part may explain how they exert their cancer-preventive effects. We report the crystallographic structures of human MIF bound to phenethylisothiocyanate and to l-sulforaphane (dietary isothiocyanates derived from watercress and broccoli, respectively) and correlate structural features of these two isothiocyanates with their second-order rate constants for MIF inactivation. We also characterize changes in the MIF structure using nuclear magnetic resonance heteronuclear single-quantum coherence spectra of these complexes and observe many changes at the subunit interface. While a number of chemical shifts do not change, many of those that change do not have features similar in magnitude or direction for the two isothiocyanates. The difference in the binding modes of these two ITCs provides a means of using structure-activity relationships to reveal insights into MIF biological interactions. The results of this study provide a framework for the development of therapeutics that target MIF.

-	~~==Function==~~	+	Structural Interactions Dictate the Kinetics of Macrophage Migration Inhibitory Factor Inhibition by Different Cancer-Preventive Isothiocyanates.,Crichlow GV, Fan C, Keeler C, Hodsdon M, Lolis EJ Biochemistry. 2012 Sep 11. PMID:22931430<ref>PMID:22931430</ref>
-	~~[[http://www.uniprot.org/uniprot/MIF_HUMAN MIF_HUMAN]] Pro-inflammatory cytokine. Involved in~~ the ~~innate immune response to bacterial pathogens. The expression~~ of ~~MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti~~-~~inflammatory activity of glucocorticoids~~. ~~Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro)~~, ~~but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance~~, ~~and whether it is important for cytokine activity~~.~~<ref>~~PMID:~~15908412</ref>~~<ref>PMID:~~17443469~~</ref>	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[3smc]] is~~ a ~~3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3SMC OCA].~~	+	</div>

-	==~~Reference~~==	+	==See Also==
-	<references ~~group="xtra"~~/><~~references~~/>	+	*[[Macrophage inhibitory factor\|Macrophage inhibitory factor]]
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Crichlow, G V.]]	+	[[Category: Crichlow, G V]]
-	[[Category: Lolis, E J.]]	+	[[Category: Lolis, E J]]
	[[Category: Cytokine]]		[[Category: Cytokine]]
	[[Category: Isomerase-isomerase inhibitor complex]]		[[Category: Isomerase-isomerase inhibitor complex]]
	[[Category: Receptor binding]]		[[Category: Receptor binding]]
	[[Category: Secreted]]		[[Category: Secreted]]

OCA at 04:28, 25 March 2013

OCA — Mon, 25 Mar 2013 04:28:34 GMT

←Older revision		Revision as of 04:28, 25 March 2013
Line 1:		Line 1:
-	[[Image:3smc.png\|left\|200px]]
-
	{{STRUCTURE_3smc\| PDB=3smc \| SCENE= }}		{{STRUCTURE_3smc\| PDB=3smc \| SCENE= }}
-
	===Macrophage Migration Inhibitory Factor (MIF) with Covalently Bound L-sulforaphane===		===Macrophage Migration Inhibitory Factor (MIF) with Covalently Bound L-sulforaphane===
-
	{{ABSTRACT_PUBMED_22931430}}		{{ABSTRACT_PUBMED_22931430}}
		+
		+	==Disease==
		+	[[http://www.uniprot.org/uniprot/MIF_HUMAN MIF_HUMAN]] Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:[http://omim.org/entry/604302 604302]]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
		+
		+	==Function==
		+	[[http://www.uniprot.org/uniprot/MIF_HUMAN MIF_HUMAN]] Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.<ref>PMID:15908412</ref><ref>PMID:17443469</ref>

	==About this Structure==		==About this Structure==
	[[3smc]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SMC OCA].		[[3smc]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SMC OCA].
		+
		+	==Reference==
		+	<references group="xtra"/><references/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Crichlow, G V.]]		[[Category: Crichlow, G V.]]

OCA at 12:38, 20 February 2013

OCA — Wed, 20 Feb 2013 12:38:09 GMT

←Older revision		Revision as of 12:38, 20 February 2013
Line 1:		Line 1:
-	[[Image:3smc.~~jpg~~\|left\|200px]]	+	[[Image:3smc.png\|left\|200px]]

	{{STRUCTURE_3smc\| PDB=3smc \| SCENE= }}		{{STRUCTURE_3smc\| PDB=3smc \| SCENE= }}

OCA at 06:07, 3 October 2012

OCA — Wed, 03 Oct 2012 06:07:34 GMT

←Older revision		Revision as of 06:07, 3 October 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3smc.jpg\|left\|200px]]

-	~~The entry~~ 3smc ~~is ON HOLD~~ ~~until Oct 26 2013~~	+	{{STRUCTURE_3smc\| PDB=3smc \| SCENE= }}

-	~~Authors: Crichlow, G.V., Lolis, E.J.~~	+	===Macrophage Migration Inhibitory Factor (MIF) with Covalently Bound L-sulforaphane===

-	~~Description: Macrophage Migration Inhibitory Factor (MIF)~~ with ~~Covalently Bound L~~-~~sulforaphane~~	+	{{ABSTRACT_PUBMED_22931430}}
		+
		+	==About this Structure==
		+	[[3smc]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SMC OCA].
		+	[[Category: Homo sapiens]]
		+	[[Category: Crichlow, G V.]]
		+	[[Category: Lolis, E J.]]
		+	[[Category: Cytokine]]
		+	[[Category: Isomerase-isomerase inhibitor complex]]
		+	[[Category: Receptor binding]]
		+	[[Category: Secreted]]

OCA at 19:52, 26 September 2012

OCA — Wed, 26 Sep 2012 19:52:32 GMT

←Older revision		Revision as of 19:52, 26 September 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3smc is ON HOLD until ~~Sep~~ 26 2013	+	The entry 3smc is ON HOLD until Oct 26 2013

	Authors: Crichlow, G.V., Lolis, E.J.		Authors: Crichlow, G.V., Lolis, E.J.

	Description: Macrophage Migration Inhibitory Factor (MIF) with Covalently Bound L-sulforaphane		Description: Macrophage Migration Inhibitory Factor (MIF) with Covalently Bound L-sulforaphane

OCA at 05:08, 27 June 2012

OCA — Wed, 27 Jun 2012 05:08:40 GMT

←Older revision		Revision as of 05:08, 27 June 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3smc is ON HOLD until ~~Jun 27~~ 2013	+	The entry 3smc is ON HOLD until Sep 26 2013

	Authors: Crichlow, G.V., Lolis, E.J.		Authors: Crichlow, G.V., Lolis, E.J.

	Description: Macrophage Migration Inhibitory Factor (MIF) with Covalently Bound L-sulforaphane		Description: Macrophage Migration Inhibitory Factor (MIF) with Covalently Bound L-sulforaphane

OCA at 07:04, 6 June 2012

OCA — Wed, 06 Jun 2012 07:04:40 GMT

←Older revision		Revision as of 07:04, 6 June 2012
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3smc is ON HOLD until ~~Paper Publication~~	+	The entry 3smc is ON HOLD until Jun 27 2013

	Authors: Crichlow, G.V., Lolis, E.J.		Authors: Crichlow, G.V., Lolis, E.J.

	Description: Macrophage Migration Inhibitory Factor (MIF) with Covalently Bound L-sulforaphane		Description: Macrophage Migration Inhibitory Factor (MIF) with Covalently Bound L-sulforaphane