3swn - Revision history

OCA at 09:57, 1 March 2024

OCA — Fri, 01 Mar 2024 09:57:49 GMT

←Older revision		Revision as of 09:57, 1 March 2024
Line 3:		Line 3:
	<StructureSection load='3swn' size='340' side='right'caption='[[3swn]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3swn' size='340' side='right'caption='[[3swn]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3swn]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Fission_yeast Fission yeast~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SWN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SWN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3swn]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SWN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SWN FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>lsm7, SPCC285.12 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~284812 Fission yeast]), lsm5, SPBC20F10.09 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=284812 Fission yeast]), lsm6, SPAC2F3.17c ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3swn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3swn OCA], [https://pdbe.org/3swn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3swn RCSB], [https://www.ebi.ac.uk/pdbsum/3swn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3swn ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3swn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3swn OCA], [https://pdbe.org/3swn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3swn RCSB], [https://www.ebi.ac.uk/pdbsum/3swn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3swn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/LSM7_SCHPO LSM7_SCHPO]] Component of LSm protein complexes, which are involved in RNA processing and may function in a chaperone-like manner. Probable component of the spliceosome. [[https://www.uniprot.org/uniprot/LSM6_SCHPO LSM6_SCHPO]] Component of LSm protein complexes, which are involved in RNA processing and may function in a chaperone-like manner, facilitating the efficient association of RNA processing factors with their substrates. Component of the cytoplasmic LSM1-LSM7 complex, which is thought to be involved in mRNA degradation by activating the decapping step in the 5'-to-3' mRNA decay pathway. Component of the nuclear LSM2-LSM8 complex, which is involved in splicing of nuclear mRNAs. LSM2-LSM8 associates with multiple snRNP complexes containing the U6 snRNA (U4/U6 di-snRNP, spliceosomal U4/U6.U5 tri-snRNP, and free U6 snRNP). It binds directly to the 3'-terminal U-tract of U6 snRNA and plays a role in the biogenesis and stability of the U6 snRNP and U4/U6 snRNP complexes. LSM2-LSM8 probably also is involved degradation of nuclear pre-mRNA by targeting them for decapping, and in processing of pre-tRNAs, pre-rRNAs and U3 snoRNA (By similarity). [[https://www.uniprot.org/uniprot/LSM5_SCHPO LSM5_SCHPO]] Component of LSm protein complexes, which are involved in RNA processing and may function in a chaperone-like manner. LSm5 is required for processing of pre-tRNAs, pre-rRNAs and U3 snoRNA (By similarity).	+	[https://www.uniprot.org/uniprot/LSM7_SCHPO LSM7_SCHPO] Component of LSm protein complexes, which are involved in RNA processing and may function in a chaperone-like manner. Probable component of the spliceosome.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The nuclear LSm2-8 (like Sm) complex and the cytoplasmic LSm1-7 complex play a central role in mRNA splicing and degradation, respectively. The LSm proteins are related to the spliceosomal Sm proteins that form a heteroheptameric ring around small nuclear RNA. The assembly process of the heptameric Sm complex is well established and involves several smaller Sm assembly intermediates. The assembly of the LSm complex, however, is less well studied. Here, we solved the 2.5 A-resolution structure of the LSm assembly intermediate that contains LSm5, LSm6, and LSm7. The three monomers display the canonical Sm fold and arrange into a hexameric LSm657-657 ring. We show that the order of the LSm proteins within the ring is consistent with the order of the related SmE, SmF, and SmG proteins in the heptameric Sm ring. Nonetheless, differences in RNA binding pockets prevent the prediction of the nucleotide binding preferences of the LSm complexes. Using high-resolution NMR spectroscopy, we confirm that LSm5, LSm6, and LSm7 also assemble into a 60-kDa hexameric ring in solution. With a combination of pull-down and NMR experiments, we show that the LSm657 complex can incorporate LSm23 in order to assemble further towards native LSm rings. Interestingly, we find that the NMR spectra of the LSm57, LSm657-657, and LSm23-657 complexes differ significantly, suggesting that the angles between the LSm building blocks change depending on the ring size of the complex. In summary, our results identify LSm657 as a plastic and functional building block on the assembly route towards the LSm1-7 and LSm2-8 complexes.	+
-		+
-	Structure of the LSm657 complex: an assembly intermediate of the LSm1-7 and LSm2-8 rings.,Mund M, Neu A, Ullmann J, Neu U, Sprangers R J Mol Biol. 2011 Nov 25;414(2):165-76. Epub 2011 Oct 6. PMID:22001694<ref>PMID:22001694</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3swn" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
-	*[[Sm-like protein\|Sm-like protein]]	+	*[[Sm-like protein 3D structures\|Sm-like protein 3D structures]]
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Fission yeast]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Mund, M]]	+	[[Category: Schizosaccharomyces pombe 972h-]]
-	[[Category: Neu, A]]	+	[[Category: Mund M]]
-	[[Category: Neu, U]]	+	[[Category: Neu A]]
-	[[Category: Sprangers, R]]	+	[[Category: Neu U]]
-	[[Category: Ullmann~~, J L]]~~	+	[[Category: Sprangers R]]
-	~~[[Category: Nuclear]]~~	+	[[Category: Ullmann JL]]
-	~~[[Category: Rna]]~~	+
-	~~[[Category: Rna binding protein]]~~	+
-	~~[[Category: Rna metabolism]]~~	+
-	~~[[Category: Sm~~]]	+

OCA at 08:13, 29 June 2022

OCA — Wed, 29 Jun 2022 08:13:45 GMT

←Older revision		Revision as of 08:13, 29 June 2022
Line 1:		Line 1:

	==Structure of the LSm657 Complex: An Assembly Intermediate of the LSm1 7 and LSm2 8 Rings==		==Structure of the LSm657 Complex: An Assembly Intermediate of the LSm1 7 and LSm2 8 Rings==
-	<StructureSection load='3swn' size='340' side='right' caption='[[3swn]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='3swn' size='340' side='right'caption='[[3swn]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3swn]] is a 12 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Fission_yeast Fission yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SWN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3SWN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3swn]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Fission_yeast Fission yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SWN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SWN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lsm7, SPCC285.12 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast]), lsm5, SPBC20F10.09 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast]), lsm6, SPAC2F3.17c ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lsm7, SPCC285.12 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast]), lsm5, SPBC20F10.09 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast]), lsm6, SPAC2F3.17c ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3swn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3swn OCA], [~~http~~://pdbe.org/3swn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3swn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3swn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3swn ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3swn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3swn OCA], [https://pdbe.org/3swn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3swn RCSB], [https://www.ebi.ac.uk/pdbsum/3swn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3swn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/LSM7_SCHPO LSM7_SCHPO]] Component of LSm protein complexes, which are involved in RNA processing and may function in a chaperone-like manner. Probable component of the spliceosome. [[~~http~~://www.uniprot.org/uniprot/LSM6_SCHPO LSM6_SCHPO]] Component of LSm protein complexes, which are involved in RNA processing and may function in a chaperone-like manner, facilitating the efficient association of RNA processing factors with their substrates. Component of the cytoplasmic LSM1-LSM7 complex, which is thought to be involved in mRNA degradation by activating the decapping step in the 5'-to-3' mRNA decay pathway. Component of the nuclear LSM2-LSM8 complex, which is involved in splicing of nuclear mRNAs. LSM2-LSM8 associates with multiple snRNP complexes containing the U6 snRNA (U4/U6 di-snRNP, spliceosomal U4/U6.U5 tri-snRNP, and free U6 snRNP). It binds directly to the 3'-terminal U-tract of U6 snRNA and plays a role in the biogenesis and stability of the U6 snRNP and U4/U6 snRNP complexes. LSM2-LSM8 probably also is involved degradation of nuclear pre-mRNA by targeting them for decapping, and in processing of pre-tRNAs, pre-rRNAs and U3 snoRNA (By similarity). [[~~http~~://www.uniprot.org/uniprot/LSM5_SCHPO LSM5_SCHPO]] Component of LSm protein complexes, which are involved in RNA processing and may function in a chaperone-like manner. LSm5 is required for processing of pre-tRNAs, pre-rRNAs and U3 snoRNA (By similarity).	+	[[https://www.uniprot.org/uniprot/LSM7_SCHPO LSM7_SCHPO]] Component of LSm protein complexes, which are involved in RNA processing and may function in a chaperone-like manner. Probable component of the spliceosome. [[https://www.uniprot.org/uniprot/LSM6_SCHPO LSM6_SCHPO]] Component of LSm protein complexes, which are involved in RNA processing and may function in a chaperone-like manner, facilitating the efficient association of RNA processing factors with their substrates. Component of the cytoplasmic LSM1-LSM7 complex, which is thought to be involved in mRNA degradation by activating the decapping step in the 5'-to-3' mRNA decay pathway. Component of the nuclear LSM2-LSM8 complex, which is involved in splicing of nuclear mRNAs. LSM2-LSM8 associates with multiple snRNP complexes containing the U6 snRNA (U4/U6 di-snRNP, spliceosomal U4/U6.U5 tri-snRNP, and free U6 snRNP). It binds directly to the 3'-terminal U-tract of U6 snRNA and plays a role in the biogenesis and stability of the U6 snRNP and U4/U6 snRNP complexes. LSM2-LSM8 probably also is involved degradation of nuclear pre-mRNA by targeting them for decapping, and in processing of pre-tRNAs, pre-rRNAs and U3 snoRNA (By similarity). [[https://www.uniprot.org/uniprot/LSM5_SCHPO LSM5_SCHPO]] Component of LSm protein complexes, which are involved in RNA processing and may function in a chaperone-like manner. LSm5 is required for processing of pre-tRNAs, pre-rRNAs and U3 snoRNA (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Fission yeast]]		[[Category: Fission yeast]]
		+	[[Category: Large Structures]]
	[[Category: Mund, M]]		[[Category: Mund, M]]
	[[Category: Neu, A]]		[[Category: Neu, A]]

OCA at 07:21, 4 August 2016

OCA — Thu, 04 Aug 2016 07:21:53 GMT

←Older revision		Revision as of 07:21, 4 August 2016
Line 1:		Line 1:
		+
	==Structure of the LSm657 Complex: An Assembly Intermediate of the LSm1 7 and LSm2 8 Rings==		==Structure of the LSm657 Complex: An Assembly Intermediate of the LSm1 7 and LSm2 8 Rings==
	<StructureSection load='3swn' size='340' side='right' caption='[[3swn]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3swn' size='340' side='right' caption='[[3swn]], [[Resolution\|resolution]] 2.50Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lsm7, SPCC285.12 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast]), lsm5, SPBC20F10.09 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast]), lsm6, SPAC2F3.17c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lsm7, SPCC285.12 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast]), lsm5, SPBC20F10.09 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast]), lsm6, SPAC2F3.17c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3swn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3swn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3swn RCSB], [http://www.ebi.ac.uk/pdbsum/3swn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3swn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3swn OCA], [http://pdbe.org/3swn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3swn RCSB], [http://www.ebi.ac.uk/pdbsum/3swn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3swn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3swn" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 06:52, 25 December 2014

OCA — Thu, 25 Dec 2014 06:52:56 GMT

←Older revision		Revision as of 06:52, 25 December 2014
Line 2:		Line 2:
	<StructureSection load='3swn' size='340' side='right' caption='[[3swn]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3swn' size='340' side='right' caption='[[3swn]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3swn]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SWN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3swn]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Fission_yeast Fission yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SWN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lsm7, SPCC285.12 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast]), lsm5, SPBC20F10.09 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast]), lsm6, SPAC2F3.17c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3swn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3swn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3swn RCSB], [http://www.ebi.ac.uk/pdbsum/3swn PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3swn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3swn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3swn RCSB], [http://www.ebi.ac.uk/pdbsum/3swn PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LSM7_SCHPO LSM7_SCHPO]] Component of LSm protein complexes, which are involved in RNA processing and may function in a chaperone-like manner. Probable component of the spliceosome. [[http://www.uniprot.org/uniprot/LSM6_SCHPO LSM6_SCHPO]] Component of LSm protein complexes, which are involved in RNA processing and may function in a chaperone-like manner, facilitating the efficient association of RNA processing factors with their substrates. Component of the cytoplasmic LSM1-LSM7 complex, which is thought to be involved in mRNA degradation by activating the decapping step in the 5'-to-3' mRNA decay pathway. Component of the nuclear LSM2-LSM8 complex, which is involved in splicing of nuclear mRNAs. LSM2-LSM8 associates with multiple snRNP complexes containing the U6 snRNA (U4/U6 di-snRNP, spliceosomal U4/U6.U5 tri-snRNP, and free U6 snRNP). It binds directly to the 3'-terminal U-tract of U6 snRNA and plays a role in the biogenesis and stability of the U6 snRNP and U4/U6 snRNP complexes. LSM2-LSM8 probably also is involved degradation of nuclear pre-mRNA by targeting them for decapping, and in processing of pre-tRNAs, pre-rRNAs and U3 snoRNA (By similarity). [[http://www.uniprot.org/uniprot/LSM5_SCHPO LSM5_SCHPO]] Component of LSm protein complexes, which are involved in RNA processing and may function in a chaperone-like manner. LSm5 is required for processing of pre-tRNAs, pre-rRNAs and U3 snoRNA (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Fission yeast]]
	[[Category: Mund, M]]		[[Category: Mund, M]]
	[[Category: Neu, A]]		[[Category: Neu, A]]

OCA at 07:30, 12 November 2014

OCA — Wed, 12 Nov 2014 07:30:27 GMT

←Older revision		Revision as of 07:30, 12 November 2014
Line 2:		Line 2:
	<StructureSection load='3swn' size='340' side='right' caption='[[3swn]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='3swn' size='340' side='right' caption='[[3swn]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3swn]] is a 12 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SWN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3swn]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SWN FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~<br>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lsm7, SPCC285.12 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 Schizosaccharomyces pombe]), lsm5, SPBC20F10.09 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 Schizosaccharomyces pombe]), lsm6, SPAC2F3.17c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 Schizosaccharomyces pombe])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3swn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3swn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3swn RCSB], [http://www.ebi.ac.uk/pdbsum/3swn PDBsum]</span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3swn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3swn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3swn RCSB], [http://www.ebi.ac.uk/pdbsum/3swn PDBsum]</span></td></tr>	+	</table>
-	<table>	+
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 13:		Line 12:
	Structure of the LSm657 complex: an assembly intermediate of the LSm1-7 and LSm2-8 rings.,Mund M, Neu A, Ullmann J, Neu U, Sprangers R J Mol Biol. 2011 Nov 25;414(2):165-76. Epub 2011 Oct 6. PMID:22001694<ref>PMID:22001694</ref>		Structure of the LSm657 complex: an assembly intermediate of the LSm1-7 and LSm2-8 rings.,Mund M, Neu A, Ullmann J, Neu U, Sprangers R J Mol Biol. 2011 Nov 25;414(2):165-76. Epub 2011 Oct 6. PMID:22001694<ref>PMID:22001694</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>

Line 22:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	~~[[Category: Schizosaccharomyces pombe]]~~	+	[[Category: Mund, M]]
-	[[Category: Mund, M.]]	+	[[Category: Neu, A]]
-	[[Category: Neu, A.]]	+	[[Category: Neu, U]]
-	[[Category: Neu, u.]]	+	[[Category: Sprangers, R]]
-	[[Category: Sprangers, R.]]	+	[[Category: Ullmann, J L]]
-	[[Category: Ullmann, J L.]]	+
	[[Category: Nuclear]]		[[Category: Nuclear]]
	[[Category: Rna]]		[[Category: Rna]]

OCA at 05:20, 5 June 2014

OCA — Thu, 05 Jun 2014 05:20:54 GMT

←Older revision		Revision as of 05:20, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3swn.~~png~~\|~~left~~\|~~200px~~]]	+	==Structure of the LSm657 Complex: An Assembly Intermediate of the LSm1 7 and LSm2 8 Rings==
		+	<StructureSection load='3swn' size='340' side='right' caption='[[3swn]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3swn]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SWN FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lsm7, SPCC285.12 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 Schizosaccharomyces pombe]), lsm5, SPBC20F10.09 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 Schizosaccharomyces pombe]), lsm6, SPAC2F3.17c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 Schizosaccharomyces pombe])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3swn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3swn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3swn RCSB], [http://www.ebi.ac.uk/pdbsum/3swn PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The nuclear LSm2-8 (like Sm) complex and the cytoplasmic LSm1-7 complex play a central role in mRNA splicing and degradation, respectively. The LSm proteins are related to the spliceosomal Sm proteins that form a heteroheptameric ring around small nuclear RNA. The assembly process of the heptameric Sm complex is well established and involves several smaller Sm assembly intermediates. The assembly of the LSm complex, however, is less well studied. Here, we solved the 2.5 A-resolution structure of the LSm assembly intermediate that contains LSm5, LSm6, and LSm7. The three monomers display the canonical Sm fold and arrange into a hexameric LSm657-657 ring. We show that the order of the LSm proteins within the ring is consistent with the order of the related SmE, SmF, and SmG proteins in the heptameric Sm ring. Nonetheless, differences in RNA binding pockets prevent the prediction of the nucleotide binding preferences of the LSm complexes. Using high-resolution NMR spectroscopy, we confirm that LSm5, LSm6, and LSm7 also assemble into a 60-kDa hexameric ring in solution. With a combination of pull-down and NMR experiments, we show that the LSm657 complex can incorporate LSm23 in order to assemble further towards native LSm rings. Interestingly, we find that the NMR spectra of the LSm57, LSm657-657, and LSm23-657 complexes differ significantly, suggesting that the angles between the LSm building blocks change depending on the ring size of the complex. In summary, our results identify LSm657 as a plastic and functional building block on the assembly route towards the LSm1-7 and LSm2-8 complexes.

-	<!--	+	Structure of the LSm657 complex: an assembly intermediate of the LSm1-7 and LSm2-8 rings.,Mund M, Neu A, Ullmann J, Neu U, Sprangers R J Mol Biol. 2011 Nov 25;414(2):165-76. Epub 2011 Oct 6. PMID:22001694<ref>PMID:22001694</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3swn"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3swn\| PDB=3swn \| SCENE= }}~~	+

-	~~===Structure~~ of the ~~LSm657 Complex: An Assembly Intermediate~~ of ~~the LSm1 7 and LSm2 8 Rings===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-		+	==See Also==
-	~~<!--~~	+	*[[Sm-like protein\|Sm-like protein]]
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22001694}}, adds the Publication Abstract to the page~~	+	== References ==
-	~~(as it appears on PubMed at http://www.pubmed.gov), where 22001694 is the PubMed ID number.~~	+	<references/>
-	~~-->~~	+	__TOC__
-	~~{{ABSTRACT_PUBMED_22001694}}~~	+	</StructureSection>
-		+
-	==~~About this Structure~~==	+
-	[[~~3swn~~]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SWN OCA].	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022001694<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Schizosaccharomyces pombe]]		[[Category: Schizosaccharomyces pombe]]
	[[Category: Mund, M.]]		[[Category: Mund, M.]]

OCA at 05:14, 4 April 2012

OCA — Wed, 04 Apr 2012 05:14:45 GMT

←Older revision		Revision as of 05:14, 4 April 2012
Line 11:		Line 11:
	===Structure of the LSm657 Complex: An Assembly Intermediate of the LSm1 7 and LSm2 8 Rings===		===Structure of the LSm657 Complex: An Assembly Intermediate of the LSm1 7 and LSm2 8 Rings===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22001694}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22001694 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22001694}}

	==About this Structure==		==About this Structure==
	[[3swn]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SWN OCA].		[[3swn]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SWN OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022001694</ref><references group="xtra"/>
	[[Category: Schizosaccharomyces pombe]]		[[Category: Schizosaccharomyces pombe]]
	[[Category: Mund, M.]]		[[Category: Mund, M.]]

OCA at 11:25, 30 October 2011

OCA — Sun, 30 Oct 2011 11:25:08 GMT

←Older revision		Revision as of 11:25, 30 October 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3swn.png\|left\|200px]]

-	The ~~entry 3swn~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3swn", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3swn\| PDB=3swn \| SCENE= }}

-	~~Authors~~: ~~Mund, M., Neu, A., Ullmann, J.L., Neu, u., Sprangers, R.~~	+	===Structure of the LSm657 Complex: An Assembly Intermediate of the LSm1 7 and LSm2 8 Rings===

-	~~Description: Crystal~~ structure of	+
		+	==About this Structure==
		+	[[3swn]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SWN OCA].
		+	[[Category: Schizosaccharomyces pombe]]
		+	[[Category: Mund, M.]]
		+	[[Category: Neu, A.]]
		+	[[Category: Neu, u.]]
		+	[[Category: Sprangers, R.]]
		+	[[Category: Ullmann, J L.]]
		+	[[Category: Nuclear]]
		+	[[Category: Rna]]
		+	[[Category: Rna binding protein]]
		+	[[Category: Rna metabolism]]
		+	[[Category: Sm]]

OCA at 06:00, 14 September 2011

OCA — Wed, 14 Sep 2011 06:00:51 GMT

←Older revision		Revision as of 06:00, 14 September 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3swn is ON HOLD	+	The entry 3swn is ON HOLD until Paper Publication

	Authors: Mund, M., Neu, A., Ullmann, J.L., Neu, u., Sprangers, R.		Authors: Mund, M., Neu, A., Ullmann, J.L., Neu, u., Sprangers, R.

-	Description: ~~CRYSTAL STRUCTURE OF~~	+	Description: Crystal structure of

OCA at 05:43, 27 July 2011

OCA — Wed, 27 Jul 2011 05:43:16 GMT

←Older revision		Revision as of 05:43, 27 July 2011
Line 3:		Line 3:
	The entry 3swn is ON HOLD		The entry 3swn is ON HOLD

-	Authors: ~~mund~~, m., ~~neu~~, a., ~~ullmann~~, j.l., ~~neu~~, u., ~~sprangers~~, r.	+	Authors: Mund, M., Neu, A., Ullmann, J.L., Neu, u., Sprangers, R.

	Description: CRYSTAL STRUCTURE OF		Description: CRYSTAL STRUCTURE OF