3t1q - Revision history

OCA at 13:14, 14 March 2024

OCA — Thu, 14 Mar 2024 13:14:40 GMT

←Older revision		Revision as of 13:14, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3t1q' size='340' side='right'caption='[[3t1q]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='3t1q' size='340' side='right'caption='[[3t1q]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t1q]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Thet8 Thet8~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T1Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T1Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t1q]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T1Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T1Q FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID~~-~~GUANYLATE+ESTER'>GNP</scene>~~, ~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[3t12\|3t12]], [[3t1o\|3t1o]], [[3t1r\|3t1r]], [[3t1s\|3t1s]], [[3t1t\|3t1t]], [[3t1v\|3t1v]], [[3t1x\|3t1x]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>TTHA1132 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~300852 THET8]), TTHA1131 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Small_monomeric_GTPase Small monomeric GTPase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.2 3.6.5.2]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t1q OCA], [https://pdbe.org/3t1q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t1q RCSB], [https://www.ebi.ac.uk/pdbsum/3t1q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t1q ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t1q OCA], [https://pdbe.org/3t1q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t1q RCSB], [https://www.ebi.ac.uk/pdbsum/3t1q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t1q ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q5SJ83_THET8 Q5SJ83_THET8]
-	The bacterium Myxococcus xanthus uses a G protein cycle to dynamically regulate the leading/lagging pole polarity axis. The G protein MglA is regulated by its GTPase-activating protein (GAP) MglB, thus resembling Ras family proteins. Here, we show structurally and biochemically that MglA undergoes a dramatic, GDP-GTP-dependent conformational change involving a screw-type forward movement of the central beta2-strand, never observed in any other G protein. This movement and complex formation with MglB repositions the conserved residues Arg53 and Gln82 into the active site. Residues required for catalysis are thus not provided by the GAP MglB, but by MglA itself. MglB is a Roadblock/LC7 protein and functions as a dimer to stimulate GTP hydrolysis in a 2:1 complex with MglA. In vivo analyses demonstrate that hydrolysis mutants abrogate Myxococcus' ability to regulate its polarity axis changing the reversal behaviour from stochastic to oscillatory and that both MglA GTPase activity and MglB GAP catalysis are essential for maintaining a proper polarity axis.	+
-		+
-	Structural analysis of the Ras-like G protein MglA and its cognate GAP MglB and implications for bacterial polarity.,Miertzschke M, Koerner C, Vetter IR, Keilberg D, Hot E, Leonardy S, Sogaard-Andersen L, Wittinghofer A EMBO J. 2011 Aug 16. doi: 10.1038/~~emboj.2011.291. PMID:21847100<ref>PMID:21847100<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3t1q" style="background-color:#fffaf0;"><~~/~~div>~~	+

	==See Also==		==See Also==
	*[[Gliding protein\|Gliding protein]]		*[[Gliding protein\|Gliding protein]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Small monomeric GTPase]]~~	+	[[Category: Thermus thermophilus HB8]]
-	~~[[Category: Thet8~~]]	+	[[Category: Koerner C]]
-	[[Category: Koerner, C]]	+	[[Category: Miertzschke M]]
-	[[Category: Miertzschke, M]]	+	[[Category: Vetter IR]]
-	[[Category: Vetter~~, I R~~]]	+	[[Category: Wittinghofer A]]
-	[[Category: Wittinghofer, A]]	+
-	~~[[Category: Alpha/beta protein]]~~	+
-	~~[[Category: Bacterial gtpase]]~~	+
-	~~[[Category: Bacterial polarity]]~~	+
-	~~[[Category: G domain containing protein]]~~	+
-	~~[[Category: Gtpase activating protein]]~~	+
-	~~[[Category: Homodimer]]~~	+
-	~~[[Category: Hydrolase-signaling protein complex]]~~	+
-	~~[[Category: Motility]]~~	+
-	~~[[Category: Pole localisation~~]]	+

OCA at 16:39, 6 July 2022

OCA — Wed, 06 Jul 2022 16:39:27 GMT

←Older revision		Revision as of 16:39, 6 July 2022
Line 1:		Line 1:

	==MglA bound to GppNHp in complex with MglB==		==MglA bound to GppNHp in complex with MglB==
-	<StructureSection load='3t1q' size='340' side='right' caption='[[3t1q]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='3t1q' size='340' side='right'caption='[[3t1q]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t1q]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T1Q OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3T1Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t1q]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T1Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T1Q FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t12\|3t12]], [[3t1o\|3t1o]], [[3t1r\|3t1r]], [[3t1s\|3t1s]], [[3t1t\|3t1t]], [[3t1v\|3t1v]], [[3t1x\|3t1x]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3t12\|3t12]], [[3t1o\|3t1o]], [[3t1r\|3t1r]], [[3t1s\|3t1s]], [[3t1t\|3t1t]], [[3t1v\|3t1v]], [[3t1x\|3t1x]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TTHA1132 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8]), TTHA1131 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TTHA1132 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8]), TTHA1131 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Small_monomeric_GTPase Small monomeric GTPase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.2 3.6.5.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Small_monomeric_GTPase Small monomeric GTPase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.2 3.6.5.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3t1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t1q OCA], [~~http~~://pdbe.org/3t1q PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3t1q RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3t1q PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3t1q ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t1q OCA], [https://pdbe.org/3t1q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t1q RCSB], [https://www.ebi.ac.uk/pdbsum/3t1q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t1q ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 3t1q" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3t1q" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Gliding protein\|Gliding protein]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Small monomeric GTPase]]		[[Category: Small monomeric GTPase]]
	[[Category: Thet8]]		[[Category: Thet8]]

OCA at 15:26, 11 August 2016

OCA — Thu, 11 Aug 2016 15:26:45 GMT

←Older revision		Revision as of 15:26, 11 August 2016
Line 1:		Line 1:
		+
	==MglA bound to GppNHp in complex with MglB==		==MglA bound to GppNHp in complex with MglB==
	<StructureSection load='3t1q' size='340' side='right' caption='[[3t1q]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='3t1q' size='340' side='right' caption='[[3t1q]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t1q]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Thermus_thermophilus Thermus thermophilus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T1Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T1Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t1q]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T1Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T1Q FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t12\|3t12]], [[3t1o\|3t1o]], [[3t1r\|3t1r]], [[3t1s\|3t1s]], [[3t1t\|3t1t]], [[3t1v\|3t1v]], [[3t1x\|3t1x]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t12\|3t12]], [[3t1o\|3t1o]], [[3t1r\|3t1r]], [[3t1s\|3t1s]], [[3t1t\|3t1t]], [[3t1v\|3t1v]], [[3t1x\|3t1x]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TTHA1132 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~274 Thermus thermophilus~~]), TTHA1131 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~274 Thermus thermophilus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TTHA1132 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8]), TTHA1131 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Small_monomeric_GTPase Small monomeric GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.2 3.6.5.2] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Small_monomeric_GTPase Small monomeric GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.2 3.6.5.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t1q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t1q RCSB], [http://www.ebi.ac.uk/pdbsum/3t1q PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t1q OCA], [http://pdbe.org/3t1q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3t1q RCSB], [http://www.ebi.ac.uk/pdbsum/3t1q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3t1q ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3t1q" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 22:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Small monomeric GTPase]]		[[Category: Small monomeric GTPase]]
-	[[Category: ~~Thermus thermophilus~~]]	+	[[Category: Thet8]]
	[[Category: Koerner, C]]		[[Category: Koerner, C]]
	[[Category: Miertzschke, M]]		[[Category: Miertzschke, M]]

OCA at 10:42, 4 January 2015

OCA — Sun, 04 Jan 2015 10:42:02 GMT

←Older revision		Revision as of 10:42, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3t1q]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T1Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T1Q FirstGlance]. <br>		<table><tr><td colspan='2'>[[3t1q]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T1Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T1Q FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t12\|3t12]], [[3t1o\|3t1o]], [[3t1r\|3t1r]], [[3t1s\|3t1s]], [[3t1t\|3t1t]], [[3t1v\|3t1v]], [[3t1x\|3t1x]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t12\|3t12]], [[3t1o\|3t1o]], [[3t1r\|3t1r]], [[3t1s\|3t1s]], [[3t1t\|3t1t]], [[3t1v\|3t1v]], [[3t1x\|3t1x]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TTHA1132 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus]), TTHA1131 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TTHA1132 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus]), TTHA1131 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Small_monomeric_GTPase Small monomeric GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.2 3.6.5.2] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Small_monomeric_GTPase Small monomeric GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.2 3.6.5.2] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t1q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t1q RCSB], [http://www.ebi.ac.uk/pdbsum/3t1q PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t1q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t1q RCSB], [http://www.ebi.ac.uk/pdbsum/3t1q PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 15:		Line 15:
	Structural analysis of the Ras-like G protein MglA and its cognate GAP MglB and implications for bacterial polarity.,Miertzschke M, Koerner C, Vetter IR, Keilberg D, Hot E, Leonardy S, Sogaard-Andersen L, Wittinghofer A EMBO J. 2011 Aug 16. doi: 10.1038/emboj.2011.291. PMID:21847100<ref>PMID:21847100</ref>		Structural analysis of the Ras-like G protein MglA and its cognate GAP MglB and implications for bacterial polarity.,Miertzschke M, Koerner C, Vetter IR, Keilberg D, Hot E, Leonardy S, Sogaard-Andersen L, Wittinghofer A EMBO J. 2011 Aug 16. doi: 10.1038/emboj.2011.291. PMID:21847100<ref>PMID:21847100</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 23:		Line 23:
	[[Category: Small monomeric GTPase]]		[[Category: Small monomeric GTPase]]
	[[Category: Thermus thermophilus]]		[[Category: Thermus thermophilus]]
-	[[Category: Koerner, C.]]	+	[[Category: Koerner, C]]
-	[[Category: Miertzschke, M.]]	+	[[Category: Miertzschke, M]]
-	[[Category: Vetter, I R.]]	+	[[Category: Vetter, I R]]
-	[[Category: Wittinghofer, A.]]	+	[[Category: Wittinghofer, A]]
	[[Category: Alpha/beta protein]]		[[Category: Alpha/beta protein]]
	[[Category: Bacterial gtpase]]		[[Category: Bacterial gtpase]]

OCA at 06:15, 5 June 2014

OCA — Thu, 05 Jun 2014 06:15:53 GMT

←Older revision		Revision as of 06:15, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3t1q.~~png~~\|~~left~~\|~~200px~~]]	+	==MglA bound to GppNHp in complex with MglB==
		+	<StructureSection load='3t1q' size='340' side='right' caption='[[3t1q]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3t1q]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T1Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T1Q FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t12\|3t12]], [[3t1o\|3t1o]], [[3t1r\|3t1r]], [[3t1s\|3t1s]], [[3t1t\|3t1t]], [[3t1v\|3t1v]], [[3t1x\|3t1x]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TTHA1132 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus]), TTHA1131 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Small_monomeric_GTPase Small monomeric GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.2 3.6.5.2] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t1q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t1q RCSB], [http://www.ebi.ac.uk/pdbsum/3t1q PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The bacterium Myxococcus xanthus uses a G protein cycle to dynamically regulate the leading/lagging pole polarity axis. The G protein MglA is regulated by its GTPase-activating protein (GAP) MglB, thus resembling Ras family proteins. Here, we show structurally and biochemically that MglA undergoes a dramatic, GDP-GTP-dependent conformational change involving a screw-type forward movement of the central beta2-strand, never observed in any other G protein. This movement and complex formation with MglB repositions the conserved residues Arg53 and Gln82 into the active site. Residues required for catalysis are thus not provided by the GAP MglB, but by MglA itself. MglB is a Roadblock/LC7 protein and functions as a dimer to stimulate GTP hydrolysis in a 2:1 complex with MglA. In vivo analyses demonstrate that hydrolysis mutants abrogate Myxococcus' ability to regulate its polarity axis changing the reversal behaviour from stochastic to oscillatory and that both MglA GTPase activity and MglB GAP catalysis are essential for maintaining a proper polarity axis.

-	~~<!-~~-	+	Structural analysis of the Ras-like G protein MglA and its cognate GAP MglB and implications for bacterial polarity.,Miertzschke M, Koerner C, Vetter IR, Keilberg D, Hot E, Leonardy S, Sogaard-Andersen L, Wittinghofer A EMBO J. 2011 Aug 16. doi: 10.1038/emboj.2011.291. PMID:21847100<ref>PMID:21847100</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3t1q"~~, ~~creates the "Structure Box" on the page.~~	+
-	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded)~~,	+
-	~~or leave the SCENE parameter empty for the default display.~~	+
-	-->	+
-	~~{{STRUCTURE_3t1q\| PDB=3t1q \| SCENE= }}~~	+

-	~~===MglA bound to GppNHp in complex with MglB===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph~~, ~~{{ABSTRACT_PUBMED_21847100}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 21847100 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_21847100}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3t1q]] is a 3 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T1Q OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:021847100<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Small monomeric GTPase]]		[[Category: Small monomeric GTPase]]
	[[Category: Thermus thermophilus]]		[[Category: Thermus thermophilus]]

OCA at 07:45, 2 November 2011

OCA — Wed, 02 Nov 2011 07:45:12 GMT

←Older revision		Revision as of 07:45, 2 November 2011
Line 1:		Line 1:
-	[[Image:3t1q.~~jpg~~\|left\|200px]]	+	[[Image:3t1q.png\|left\|200px]]

	<!--		<!--

OCA at 06:50, 31 August 2011

OCA — Wed, 31 Aug 2011 06:50:32 GMT

←Older revision		Revision as of 06:50, 31 August 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3t1q.jpg\|left\|200px]]

-	The ~~entry 3t1q~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3t1q", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3t1q\| PDB=3t1q \| SCENE= }}

-	~~Authors: Miertzschke, M., Vetter, I.R., Koerner, C., Wittinghofer, A.~~	+	===MglA bound to GppNHp in complex with MglB===

-	~~Description: MglA bound~~ to ~~GppNHp in complex~~ with ~~MglB~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21847100}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21847100 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21847100}}
		+
		+	==About this Structure==
		+	[[3t1q]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T1Q OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021847100</ref><references group="xtra"/>
		+	[[Category: Small monomeric GTPase]]
		+	[[Category: Thermus thermophilus]]
		+	[[Category: Koerner, C.]]
		+	[[Category: Miertzschke, M.]]
		+	[[Category: Vetter, I R.]]
		+	[[Category: Wittinghofer, A.]]
		+	[[Category: Alpha/beta protein]]
		+	[[Category: Bacterial gtpase]]
		+	[[Category: Bacterial polarity]]
		+	[[Category: G domain containing protein]]
		+	[[Category: Gtpase activating protein]]
		+	[[Category: Homodimer]]
		+	[[Category: Hydrolase-signaling protein complex]]
		+	[[Category: Motility]]
		+	[[Category: Pole localisation]]

OCA at 05:52, 17 August 2011

OCA — Wed, 17 Aug 2011 05:52:43 GMT

←Older revision		Revision as of 05:52, 17 August 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3t1q is ON HOLD	+	The entry 3t1q is ON HOLD until Paper Publication

	Authors: Miertzschke, M., Vetter, I.R., Koerner, C., Wittinghofer, A.		Authors: Miertzschke, M., Vetter, I.R., Koerner, C., Wittinghofer, A.

	Description: MglA bound to GppNHp in complex with MglB		Description: MglA bound to GppNHp in complex with MglB

OCA: Protected "3t1q" [edit=sysop:move=sysop]

OCA — Wed, 03 Aug 2011 08:23:30 GMT

Protected "3t1q" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:23, 3 August 2011

OCA: New page: '''Unreleased structure''' The entry 3t1q is ON HOLD Authors: Miertzschke, M., Vetter, I.R., Koerner, C., Wittinghofer, A. Description: MglA bound to GppNHp in complex with MglB

OCA — Wed, 03 Aug 2011 08:23:29 GMT

New page: '''Unreleased structure''' The entry 3t1q is ON HOLD Authors: Miertzschke, M., Vetter, I.R., Koerner, C., Wittinghofer, A. Description: MglA bound to GppNHp in complex with MglB

New page

'''Unreleased structure'''

The entry 3t1q is ON HOLD

Authors: Miertzschke, M., Vetter, I.R., Koerner, C., Wittinghofer, A.

Description: MglA bound to GppNHp in complex with MglB