3t1x - Revision history

OCA at 13:15, 14 March 2024

OCA — Thu, 14 Mar 2024 13:15:04 GMT

←Older revision		Revision as of 13:15, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3t1x' size='340' side='right'caption='[[3t1x]], [[Resolution\|resolution]] 2.91Å' scene=''>		<StructureSection load='3t1x' size='340' side='right'caption='[[3t1x]], [[Resolution\|resolution]] 2.91Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t1x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Thet8 Thet8~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T1X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T1X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t1x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T1X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T1X FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[3t12\|3t12]], [[3t1o\|3t1o]], [[3t1q\|3t1q]], [[3t1r\|3t1r]], [[3t1s\|3t1s]], [[3t1t\|3t1t]], [[3t1v\|3t1v]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.91Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">TTHA1131 ([https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=300852 THET8])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t1x OCA], [https://pdbe.org/3t1x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t1x RCSB], [https://www.ebi.ac.uk/pdbsum/3t1x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t1x ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t1x OCA], [https://pdbe.org/3t1x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t1x RCSB], [https://www.ebi.ac.uk/pdbsum/3t1x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t1x ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q5SJ83_THET8 Q5SJ83_THET8]
-	The bacterium Myxococcus xanthus uses a G protein cycle to dynamically regulate the leading/lagging pole polarity axis. The G protein MglA is regulated by its GTPase-activating protein (GAP) MglB, thus resembling Ras family proteins. Here, we show structurally and biochemically that MglA undergoes a dramatic, GDP-GTP-dependent conformational change involving a screw-type forward movement of the central beta2-strand, never observed in any other G protein. This movement and complex formation with MglB repositions the conserved residues Arg53 and Gln82 into the active site. Residues required for catalysis are thus not provided by the GAP MglB, but by MglA itself. MglB is a Roadblock/LC7 protein and functions as a dimer to stimulate GTP hydrolysis in a 2:1 complex with MglA. In vivo analyses demonstrate that hydrolysis mutants abrogate Myxococcus' ability to regulate its polarity axis changing the reversal behaviour from stochastic to oscillatory and that both MglA GTPase activity and MglB GAP catalysis are essential for maintaining a proper polarity axis.	+
-		+
-	Structural analysis of the Ras-like G protein MglA and its cognate GAP MglB and implications for bacterial polarity.,Miertzschke M, Koerner C, Vetter IR, Keilberg D, Hot E, Leonardy S, Sogaard-Andersen L, Wittinghofer A EMBO J. 2011 Aug 16. doi: 10.1038/~~emboj.2011.291. PMID:21847100<ref>PMID:21847100<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3t1x" style="background-color:#fffaf0;"><~~/~~div>~~	+

	==See Also==		==See Also==
	*[[Gliding protein\|Gliding protein]]		*[[Gliding protein\|Gliding protein]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Thet8~~]]	+	[[Category: Thermus thermophilus HB8]]
-	[[Category: Koerner, C]]	+	[[Category: Koerner C]]
-	[[Category: Miertzschke, M]]	+	[[Category: Miertzschke M]]
-	[[Category: Vetter~~, I R~~]]	+	[[Category: Vetter IR]]
-	[[Category: Wittinghofer, A]]	+	[[Category: Wittinghofer A]]
-	~~[[Category: Alpha/beta protein]]~~	+
-	~~[[Category: Bacterial polarity]]~~	+
-	~~[[Category: Catalytic gap domain]]~~	+
-	~~[[Category: Gtpase activating protein]]~~	+
-	~~[[Category: Motility]]~~	+
-	~~[[Category: Signaling protein~~]]	+

OCA at 16:39, 6 July 2022

OCA — Wed, 06 Jul 2022 16:39:40 GMT

←Older revision		Revision as of 16:39, 6 July 2022
Line 1:		Line 1:

	==MglB R124A E127A Monomer==		==MglB R124A E127A Monomer==
-	<StructureSection load='3t1x' size='340' side='right' caption='[[3t1x]], [[Resolution\|resolution]] 2.91Å' scene=''>	+	<StructureSection load='3t1x' size='340' side='right'caption='[[3t1x]], [[Resolution\|resolution]] 2.91Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t1x]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T1X OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3T1X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t1x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T1X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T1X FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t12\|3t12]], [[3t1o\|3t1o]], [[3t1q\|3t1q]], [[3t1r\|3t1r]], [[3t1s\|3t1s]], [[3t1t\|3t1t]], [[3t1v\|3t1v]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3t12\|3t12]], [[3t1o\|3t1o]], [[3t1q\|3t1q]], [[3t1r\|3t1r]], [[3t1s\|3t1s]], [[3t1t\|3t1t]], [[3t1v\|3t1v]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TTHA1131 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TTHA1131 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3t1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t1x OCA], [~~http~~://pdbe.org/3t1x PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3t1x RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3t1x PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3t1x ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t1x OCA], [https://pdbe.org/3t1x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t1x RCSB], [https://www.ebi.ac.uk/pdbsum/3t1x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t1x ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 17:
	</div>		</div>
	<div class="pdbe-citations 3t1x" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 3t1x" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Gliding protein\|Gliding protein]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Thet8]]		[[Category: Thet8]]
	[[Category: Koerner, C]]		[[Category: Koerner, C]]

OCA at 12:51, 11 August 2016

OCA — Thu, 11 Aug 2016 12:51:14 GMT

←Older revision		Revision as of 12:51, 11 August 2016
Line 1:		Line 1:
		+
	==MglB R124A E127A Monomer==		==MglB R124A E127A Monomer==
	<StructureSection load='3t1x' size='340' side='right' caption='[[3t1x]], [[Resolution\|resolution]] 2.91Å' scene=''>		<StructureSection load='3t1x' size='340' side='right' caption='[[3t1x]], [[Resolution\|resolution]] 2.91Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t1x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Thermus_thermophilus Thermus thermophilus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T1X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T1X FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t1x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T1X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T1X FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t12\|3t12]], [[3t1o\|3t1o]], [[3t1q\|3t1q]], [[3t1r\|3t1r]], [[3t1s\|3t1s]], [[3t1t\|3t1t]], [[3t1v\|3t1v]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t12\|3t12]], [[3t1o\|3t1o]], [[3t1q\|3t1q]], [[3t1r\|3t1r]], [[3t1s\|3t1s]], [[3t1t\|3t1t]], [[3t1v\|3t1v]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TTHA1131 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~274 Thermus thermophilus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TTHA1131 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t1x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t1x RCSB], [http://www.ebi.ac.uk/pdbsum/3t1x PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t1x OCA], [http://pdbe.org/3t1x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3t1x RCSB], [http://www.ebi.ac.uk/pdbsum/3t1x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3t1x ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3t1x" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Thermus thermophilus~~]]	+	[[Category: Thet8]]
	[[Category: Koerner, C]]		[[Category: Koerner, C]]
	[[Category: Miertzschke, M]]		[[Category: Miertzschke, M]]

OCA at 09:44, 4 January 2015

OCA — Sun, 04 Jan 2015 09:44:05 GMT

←Older revision		Revision as of 09:44, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3t1x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T1X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T1X FirstGlance]. <br>		<table><tr><td colspan='2'>[[3t1x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T1X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T1X FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t12\|3t12]], [[3t1o\|3t1o]], [[3t1q\|3t1q]], [[3t1r\|3t1r]], [[3t1s\|3t1s]], [[3t1t\|3t1t]], [[3t1v\|3t1v]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t12\|3t12]], [[3t1o\|3t1o]], [[3t1q\|3t1q]], [[3t1r\|3t1r]], [[3t1s\|3t1s]], [[3t1t\|3t1t]], [[3t1v\|3t1v]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TTHA1131 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TTHA1131 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t1x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t1x RCSB], [http://www.ebi.ac.uk/pdbsum/3t1x PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t1x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t1x RCSB], [http://www.ebi.ac.uk/pdbsum/3t1x PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 13:		Line 13:
	Structural analysis of the Ras-like G protein MglA and its cognate GAP MglB and implications for bacterial polarity.,Miertzschke M, Koerner C, Vetter IR, Keilberg D, Hot E, Leonardy S, Sogaard-Andersen L, Wittinghofer A EMBO J. 2011 Aug 16. doi: 10.1038/emboj.2011.291. PMID:21847100<ref>PMID:21847100</ref>		Structural analysis of the Ras-like G protein MglA and its cognate GAP MglB and implications for bacterial polarity.,Miertzschke M, Koerner C, Vetter IR, Keilberg D, Hot E, Leonardy S, Sogaard-Andersen L, Wittinghofer A EMBO J. 2011 Aug 16. doi: 10.1038/emboj.2011.291. PMID:21847100<ref>PMID:21847100</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 20:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Thermus thermophilus]]		[[Category: Thermus thermophilus]]
-	[[Category: Koerner, C.]]	+	[[Category: Koerner, C]]
-	[[Category: Miertzschke, M.]]	+	[[Category: Miertzschke, M]]
-	[[Category: Vetter, I R.]]	+	[[Category: Vetter, I R]]
-	[[Category: Wittinghofer, A.]]	+	[[Category: Wittinghofer, A]]
	[[Category: Alpha/beta protein]]		[[Category: Alpha/beta protein]]
	[[Category: Bacterial polarity]]		[[Category: Bacterial polarity]]

OCA at 06:18, 5 June 2014

OCA — Thu, 05 Jun 2014 06:18:46 GMT

←Older revision		Revision as of 06:18, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3t1x.~~png~~\|~~left~~\|~~200px~~]]	+	==MglB R124A E127A Monomer==
		+	<StructureSection load='3t1x' size='340' side='right' caption='[[3t1x]], [[Resolution\|resolution]] 2.91Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3t1x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T1X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T1X FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t12\|3t12]], [[3t1o\|3t1o]], [[3t1q\|3t1q]], [[3t1r\|3t1r]], [[3t1s\|3t1s]], [[3t1t\|3t1t]], [[3t1v\|3t1v]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TTHA1131 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t1x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t1x RCSB], [http://www.ebi.ac.uk/pdbsum/3t1x PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The bacterium Myxococcus xanthus uses a G protein cycle to dynamically regulate the leading/lagging pole polarity axis. The G protein MglA is regulated by its GTPase-activating protein (GAP) MglB, thus resembling Ras family proteins. Here, we show structurally and biochemically that MglA undergoes a dramatic, GDP-GTP-dependent conformational change involving a screw-type forward movement of the central beta2-strand, never observed in any other G protein. This movement and complex formation with MglB repositions the conserved residues Arg53 and Gln82 into the active site. Residues required for catalysis are thus not provided by the GAP MglB, but by MglA itself. MglB is a Roadblock/LC7 protein and functions as a dimer to stimulate GTP hydrolysis in a 2:1 complex with MglA. In vivo analyses demonstrate that hydrolysis mutants abrogate Myxococcus' ability to regulate its polarity axis changing the reversal behaviour from stochastic to oscillatory and that both MglA GTPase activity and MglB GAP catalysis are essential for maintaining a proper polarity axis.

-	~~<!-~~-	+	Structural analysis of the Ras-like G protein MglA and its cognate GAP MglB and implications for bacterial polarity.,Miertzschke M, Koerner C, Vetter IR, Keilberg D, Hot E, Leonardy S, Sogaard-Andersen L, Wittinghofer A EMBO J. 2011 Aug 16. doi: 10.1038/emboj.2011.291. PMID:21847100<ref>PMID:21847100</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3t1x"~~, ~~creates the "Structure Box" on the page.~~	+
-	~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded)~~,	+
-	~~or leave the SCENE parameter empty for the default display.~~	+
-	-->	+
-	~~{{STRUCTURE_3t1x\| PDB=3t1x \| SCENE= }}~~	+

-	~~===MglB R124A E127A Monomer===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph~~, ~~{{ABSTRACT_PUBMED_21847100}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 21847100 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_21847100}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3t1x]] is a 1 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T1X OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:021847100<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Thermus thermophilus]]		[[Category: Thermus thermophilus]]
	[[Category: Koerner, C.]]		[[Category: Koerner, C.]]

OCA at 07:27, 2 November 2011

OCA — Wed, 02 Nov 2011 07:27:19 GMT

←Older revision		Revision as of 07:27, 2 November 2011
Line 1:		Line 1:
-	[[Image:3t1x.~~jpg~~\|left\|200px]]	+	[[Image:3t1x.png\|left\|200px]]

	<!--		<!--

OCA at 06:43, 31 August 2011

OCA — Wed, 31 Aug 2011 06:43:10 GMT

←Older revision		Revision as of 06:43, 31 August 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3t1x.jpg\|left\|200px]]

-	The ~~entry 3t1x~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3t1x", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3t1x\| PDB=3t1x \| SCENE= }}

-	~~Authors: Miertzschke, M., Vetter, I.R., Koerner, C., Wittinghofer, A.~~	+	===MglB R124A E127A Monomer===

-	~~Description~~: ~~MglB R124A E127A Monomer~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21847100}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21847100 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21847100}}
		+
		+	==About this Structure==
		+	[[3t1x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T1X OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021847100</ref><references group="xtra"/>
		+	[[Category: Thermus thermophilus]]
		+	[[Category: Koerner, C.]]
		+	[[Category: Miertzschke, M.]]
		+	[[Category: Vetter, I R.]]
		+	[[Category: Wittinghofer, A.]]
		+	[[Category: Alpha/beta protein]]
		+	[[Category: Bacterial polarity]]
		+	[[Category: Catalytic gap domain]]
		+	[[Category: Gtpase activating protein]]
		+	[[Category: Motility]]
		+	[[Category: Signaling protein]]

OCA at 05:52, 17 August 2011

OCA — Wed, 17 Aug 2011 05:52:54 GMT

←Older revision		Revision as of 05:52, 17 August 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3t1x is ON HOLD	+	The entry 3t1x is ON HOLD until Paper Publication

	Authors: Miertzschke, M., Vetter, I.R., Koerner, C., Wittinghofer, A.		Authors: Miertzschke, M., Vetter, I.R., Koerner, C., Wittinghofer, A.

	Description: MglB R124A E127A Monomer		Description: MglB R124A E127A Monomer

OCA: Protected "3t1x" [edit=sysop:move=sysop]

OCA — Wed, 03 Aug 2011 08:23:42 GMT

Protected "3t1x" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:23, 3 August 2011

OCA: New page: '''Unreleased structure''' The entry 3t1x is ON HOLD Authors: Miertzschke, M., Vetter, I.R., Koerner, C., Wittinghofer, A. Description: MglB R124A E127A Monomer

OCA — Wed, 03 Aug 2011 08:23:41 GMT

New page: '''Unreleased structure''' The entry 3t1x is ON HOLD Authors: Miertzschke, M., Vetter, I.R., Koerner, C., Wittinghofer, A. Description: MglB R124A E127A Monomer

New page

'''Unreleased structure'''

The entry 3t1x is ON HOLD

Authors: Miertzschke, M., Vetter, I.R., Koerner, C., Wittinghofer, A.

Description: MglB R124A E127A Monomer