3t25 - Revision history

OCA at 13:15, 14 March 2024

OCA — Thu, 14 Mar 2024 13:15:17 GMT

←Older revision		Revision as of 13:15, 14 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3t25]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T25 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T25 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3t25]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T25 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T25 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TMO:TRIMETHYLAMINE+OXIDE'>TMO</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3t26\|3t26]], [[3t27\|3t27]], [[3t28\|3t28]], [[3t29\|3t29]], [[3t2a\|3t2a]], [[3t2h\|3t2h]], [[3t2i\|3t2i]], [[3t2j\|3t2j]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TMO:TRIMETHYLAMINE+OXIDE'>TMO</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t25 OCA], [https://pdbe.org/3t25 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t25 RCSB], [https://www.ebi.ac.uk/pdbsum/3t25 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t25 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t25 OCA], [https://pdbe.org/3t25 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t25 RCSB], [https://www.ebi.ac.uk/pdbsum/3t25 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t25 ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/TRY1_BOVIN TRY1_BOVIN]
-	Both crystallization and cryoprotection are often bottlenecks for high-resolution X-ray structure determination of macromolecules. Methylamine osmolytes are known stabilizers of protein structure. One such osmolyte, trimethylamine N-oxide (TMAO), has seen occasional use as an additive to improve macromolecular crystal quality and has recently been shown to be an effective cryoprotective agent for low-temperature data collection. Here, TMAO and the related osmolytes sarcosine and betaine are investigated as primary precipitating agents for protein crystal growth. Crystallization experiments were undertaken with 14 proteins. Using TMAO, seven proteins crystallized in a total of 13 crystal forms, including a new tetragonal crystal form of trypsin. The crystals diffracted well, and eight of the 13 crystal forms could be effectively cryocooled as grown with TMAO as an in situ cryoprotective agent. Sarcosine and betaine produced crystals of four and two of the 14 proteins, respectively. In addition to TMAO, sarcosine and betaine were effective post-crystallization cryoprotective agents for two different crystal forms of thermolysin. Precipitation reactions of TMAO with several transition-metal ions (Fe(3+), Co(2+), Cu(2+) and Zn(2+)) did not occur with sarcosine or betaine and were inhibited for TMAO at lower pH. Structures of proteins from TMAO-grown crystals and from crystals soaked in TMAO, sarcosine or betaine were determined, showing osmolyte binding in five of the 12 crystals tested. When an osmolyte was shown to bind, it did so near the protein surface, interacting with water molecules, side chains and backbone atoms, often at crystal contacts.	+
-		+
-	The use of trimethylamine N-oxide as a primary precipitating agent and related methylamine osmolytes as cryoprotective agents for macromolecular crystallography.,Marshall H, Venkat M, Hti Lar Seng NS, Cahn J, Juers DH Acta Crystallogr D Biol Crystallogr. 2012 Jan;68(Pt 1):~~69-81. Epub 2011, Dec 9. PMID:22194335<ref>PMID:22194335<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3t25" style="background-color:#fffaf0;"><~~/~~div>~~	+

	==See Also==		==See Also==
	*[[Trypsin 3D structures\|Trypsin 3D structures]]		*[[Trypsin 3D structures\|Trypsin 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Trypsin]]~~	+	[[Category: Cahn J]]
-	[[Category: Cahn, J]]	+	[[Category: Juers D]]
-	[[Category: Juers, D]]	+	[[Category: Marshall H]]
-	[[Category: Marshall, H]]	+	[[Category: Venkat M]]
-	[[Category: Venkat, M]]	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Protease~~]]	+

OCA at 16:39, 6 July 2022

OCA — Wed, 06 Jul 2022 16:39:48 GMT

←Older revision		Revision as of 16:39, 6 July 2022
Line 1:		Line 1:

	==TMAO-grown orthorhombic trypsin (bovine)==		==TMAO-grown orthorhombic trypsin (bovine)==
-	<StructureSection load='3t25' size='340' side='right' caption='[[3t25]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='3t25' size='340' side='right'caption='[[3t25]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t25]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T25 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3T25 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t25]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T25 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T25 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TMO:TRIMETHYLAMINE+OXIDE'>TMO</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TMO:TRIMETHYLAMINE+OXIDE'>TMO</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t26\|3t26]], [[3t27\|3t27]], [[3t28\|3t28]], [[3t29\|3t29]], [[3t2a\|3t2a]], [[3t2h\|3t2h]], [[3t2i\|3t2i]], [[3t2j\|3t2j]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3t26\|3t26]], [[3t27\|3t27]], [[3t28\|3t28]], [[3t29\|3t29]], [[3t2a\|3t2a]], [[3t2h\|3t2h]], [[3t2i\|3t2i]], [[3t2j\|3t2j]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3t25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t25 OCA], [~~http~~://pdbe.org/3t25 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3t25 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3t25 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3t25 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t25 OCA], [https://pdbe.org/3t25 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t25 RCSB], [https://www.ebi.ac.uk/pdbsum/3t25 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t25 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 20:

	==See Also==		==See Also==
-	*[[Trypsin\|Trypsin]]	+	*[[Trypsin 3D structures\|Trypsin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 26:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
		+	[[Category: Large Structures]]
	[[Category: Trypsin]]		[[Category: Trypsin]]
	[[Category: Cahn, J]]		[[Category: Cahn, J]]

OCA at 11:21, 10 December 2016

OCA — Sat, 10 Dec 2016 11:21:17 GMT

←Older revision		Revision as of 11:21, 10 December 2016
Line 1:		Line 1:
		+
	==TMAO-grown orthorhombic trypsin (bovine)==		==TMAO-grown orthorhombic trypsin (bovine)==
	<StructureSection load='3t25' size='340' side='right' caption='[[3t25]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='3t25' size='340' side='right' caption='[[3t25]], [[Resolution\|resolution]] 1.70Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t26\|3t26]], [[3t27\|3t27]], [[3t28\|3t28]], [[3t29\|3t29]], [[3t2a\|3t2a]], [[3t2h\|3t2h]], [[3t2i\|3t2i]], [[3t2j\|3t2j]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t26\|3t26]], [[3t27\|3t27]], [[3t28\|3t28]], [[3t29\|3t29]], [[3t2a\|3t2a]], [[3t2h\|3t2h]], [[3t2i\|3t2i]], [[3t2j\|3t2j]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t25 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t25 RCSB], [http://www.ebi.ac.uk/pdbsum/3t25 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t25 OCA], [http://pdbe.org/3t25 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3t25 RCSB], [http://www.ebi.ac.uk/pdbsum/3t25 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3t25 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 16:		Line 17:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3t25" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 13:23, 9 December 2014

OCA — Tue, 09 Dec 2014 13:23:26 GMT

←Older revision		Revision as of 13:23, 9 December 2014
Line 1:		Line 1:
-	[[~~Image:~~3t25.~~png~~\|~~left~~\|~~200px~~]]	+	==TMAO-grown orthorhombic trypsin (bovine)==
		+	<StructureSection load='3t25' size='340' side='right' caption='[[3t25]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3t25]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T25 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T25 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TMO:TRIMETHYLAMINE+OXIDE'>TMO</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t26\|3t26]], [[3t27\|3t27]], [[3t28\|3t28]], [[3t29\|3t29]], [[3t2a\|3t2a]], [[3t2h\|3t2h]], [[3t2i\|3t2i]], [[3t2j\|3t2j]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t25 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t25 RCSB], [http://www.ebi.ac.uk/pdbsum/3t25 PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Both crystallization and cryoprotection are often bottlenecks for high-resolution X-ray structure determination of macromolecules. Methylamine osmolytes are known stabilizers of protein structure. One such osmolyte, trimethylamine N-oxide (TMAO), has seen occasional use as an additive to improve macromolecular crystal quality and has recently been shown to be an effective cryoprotective agent for low-temperature data collection. Here, TMAO and the related osmolytes sarcosine and betaine are investigated as primary precipitating agents for protein crystal growth. Crystallization experiments were undertaken with 14 proteins. Using TMAO, seven proteins crystallized in a total of 13 crystal forms, including a new tetragonal crystal form of trypsin. The crystals diffracted well, and eight of the 13 crystal forms could be effectively cryocooled as grown with TMAO as an in situ cryoprotective agent. Sarcosine and betaine produced crystals of four and two of the 14 proteins, respectively. In addition to TMAO, sarcosine and betaine were effective post-crystallization cryoprotective agents for two different crystal forms of thermolysin. Precipitation reactions of TMAO with several transition-metal ions (Fe(3+), Co(2+), Cu(2+) and Zn(2+)) did not occur with sarcosine or betaine and were inhibited for TMAO at lower pH. Structures of proteins from TMAO-grown crystals and from crystals soaked in TMAO, sarcosine or betaine were determined, showing osmolyte binding in five of the 12 crystals tested. When an osmolyte was shown to bind, it did so near the protein surface, interacting with water molecules, side chains and backbone atoms, often at crystal contacts.

-	~~{{STRUCTURE_3t25\| PDB=3t25 \| SCENE= }}~~	+	The use of trimethylamine N-oxide as a primary precipitating agent and related methylamine osmolytes as cryoprotective agents for macromolecular crystallography.,Marshall H, Venkat M, Hti Lar Seng NS, Cahn J, Juers DH Acta Crystallogr D Biol Crystallogr. 2012 Jan;68(Pt 1):69-81. Epub 2011, Dec 9. PMID:22194335<ref>PMID:22194335</ref>

-	~~===TMAO-grown orthorhombic trypsin (bovine)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22194335}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3t25]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bos_taurus Bos taurus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3T25 OCA].~~	+

	==See Also==		==See Also==
	*[[Trypsin\|Trypsin]]		*[[Trypsin\|Trypsin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:022194335<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Trypsin]]		[[Category: Trypsin]]
-	[[Category: Cahn, J.]]	+	[[Category: Cahn, J]]
-	[[Category: Juers, D.]]	+	[[Category: Juers, D]]
-	[[Category: Marshall, H.]]	+	[[Category: Marshall, H]]
-	[[Category: Venkat, M.]]	+	[[Category: Venkat, M]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Protease]]		[[Category: Protease]]

OCA at 12:10, 20 October 2012

OCA — Sat, 20 Oct 2012 12:10:13 GMT

←Older revision		Revision as of 12:10, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3t25]] is a 1 chain structure ~~of [[Trypsin]]~~ with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T25 OCA].	+	[[3t25]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T25 OCA].

	==See Also==		==See Also==

OCA at 07:50, 26 July 2012

OCA — Thu, 26 Jul 2012 07:50:09 GMT

←Older revision		Revision as of 07:50, 26 July 2012
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	[[Image:3t25.png\|left\|200px]]		[[Image:3t25.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3t25", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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	{{STRUCTURE_3t25\| PDB=3t25 \| SCENE= }}		{{STRUCTURE_3t25\| PDB=3t25 \| SCENE= }}

	===TMAO-grown orthorhombic trypsin (bovine)===		===TMAO-grown orthorhombic trypsin (bovine)===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_22194335}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 22194335 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_22194335}}		{{ABSTRACT_PUBMED_22194335}}

	==About this Structure==		==About this Structure==
-	[[3t25]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T25 OCA].	+	[[3t25]] is a 1 chain structure of [[Trypsin]] with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T25 OCA].
		+
		+	==See Also==
		+	*[[Trypsin\|Trypsin]]

	==Reference==		==Reference==

OCA at 05:42, 11 January 2012

OCA — Wed, 11 Jan 2012 05:42:19 GMT

←Older revision		Revision as of 05:42, 11 January 2012
Line 1:		Line 1:
-	[[Image:3t25.~~jpg~~\|left\|200px]]	+	[[Image:3t25.png\|left\|200px]]

	<!--		<!--
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	===TMAO-grown orthorhombic trypsin (bovine)===		===TMAO-grown orthorhombic trypsin (bovine)===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22194335}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22194335 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22194335}}

	==About this Structure==		==About this Structure==
	[[3t25]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T25 OCA].		[[3t25]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T25 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022194335</ref><references group="xtra"/>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Trypsin]]		[[Category: Trypsin]]

OCA at 07:39, 28 December 2011

OCA — Wed, 28 Dec 2011 07:39:34 GMT

←Older revision		Revision as of 07:39, 28 December 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3t25.jpg\|left\|200px]]

-	The ~~entry 3t25~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3t25", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3t25\| PDB=3t25 \| SCENE= }}

-	~~Authors: Cahn, J., Venkat, M., Marshall, H., Juers, D.~~	+	===TMAO-grown orthorhombic trypsin (bovine)===

-	~~Description~~: ~~TMAO~~-~~grown orthorhombic trypsin (bovine)~~	+
		+	==About this Structure==
		+	[[3t25]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T25 OCA].
		+	[[Category: Bos taurus]]
		+	[[Category: Trypsin]]
		+	[[Category: Cahn, J.]]
		+	[[Category: Juers, D.]]
		+	[[Category: Marshall, H.]]
		+	[[Category: Venkat, M.]]
		+	[[Category: Hydrolase]]
		+	[[Category: Protease]]

OCA at 05:33, 10 August 2011

OCA — Wed, 10 Aug 2011 05:33:19 GMT

←Older revision		Revision as of 05:33, 10 August 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3t25 is ON HOLD	+	The entry 3t25 is ON HOLD until Paper Publication

-	Authors: Cahn, J., Venkat, M., Marshall, H.	+	Authors: Cahn, J., Venkat, M., Marshall, H., Juers, D.

	Description: TMAO-grown orthorhombic trypsin (bovine)		Description: TMAO-grown orthorhombic trypsin (bovine)

OCA: Protected "3t25" [edit=sysop:move=sysop]

OCA — Wed, 03 Aug 2011 08:23:53 GMT

Protected "3t25" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:23, 3 August 2011