3t2a - Revision history

OCA at 16:39, 6 July 2022

OCA — Wed, 06 Jul 2022 16:39:56 GMT

←Older revision		Revision as of 16:39, 6 July 2022
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	==TMAO-grown cubic insulin (porcine)==		==TMAO-grown cubic insulin (porcine)==
-	<StructureSection load='3t2a' size='340' side='right' caption='[[3t2a]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='3t2a' size='340' side='right'caption='[[3t2a]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t2a]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T2A OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3T2A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t2a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T2A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T2A FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TMO:TRIMETHYLAMINE+OXIDE'>TMO</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TMO:TRIMETHYLAMINE+OXIDE'>TMO</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t25\|3t25]], [[3t26\|3t26]], [[3t27\|3t27]], [[3t28\|3t28]], [[3t29\|3t29]], [[3t2h\|3t2h]], [[3t2i\|3t2i]], [[3t2j\|3t2j]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3t25\|3t25]], [[3t26\|3t26]], [[3t27\|3t27]], [[3t28\|3t28]], [[3t29\|3t29]], [[3t2h\|3t2h]], [[3t2i\|3t2i]], [[3t2j\|3t2j]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3t2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t2a OCA], [~~http~~://pdbe.org/3t2a PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3t2a RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3t2a PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3t2a ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t2a OCA], [https://pdbe.org/3t2a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t2a RCSB], [https://www.ebi.ac.uk/pdbsum/3t2a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t2a ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/INS_PIG INS_PIG]] Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.	+	[[https://www.uniprot.org/uniprot/INS_PIG INS_PIG]] Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 21:

	==See Also==		==See Also==
-	*[[~~Molecular Playground/~~Insulin\|~~Molecular Playground/~~Insulin]]	+	*[[Insulin 3D Structures\|Insulin 3D Structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]
	[[Category: Cahn, J]]		[[Category: Cahn, J]]

OCA at 07:35, 5 August 2016

OCA — Fri, 05 Aug 2016 07:35:12 GMT

←Older revision		Revision as of 07:35, 5 August 2016
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		+
	==TMAO-grown cubic insulin (porcine)==		==TMAO-grown cubic insulin (porcine)==
	<StructureSection load='3t2a' size='340' side='right' caption='[[3t2a]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='3t2a' size='340' side='right' caption='[[3t2a]], [[Resolution\|resolution]] 2.10Å' scene=''>
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TMO:TRIMETHYLAMINE+OXIDE'>TMO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TMO:TRIMETHYLAMINE+OXIDE'>TMO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t25\|3t25]], [[3t26\|3t26]], [[3t27\|3t27]], [[3t28\|3t28]], [[3t29\|3t29]], [[3t2h\|3t2h]], [[3t2i\|3t2i]], [[3t2j\|3t2j]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t25\|3t25]], [[3t26\|3t26]], [[3t27\|3t27]], [[3t28\|3t28]], [[3t29\|3t29]], [[3t2h\|3t2h]], [[3t2i\|3t2i]], [[3t2j\|3t2j]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t2a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t2a RCSB], [http://www.ebi.ac.uk/pdbsum/3t2a PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t2a OCA], [http://pdbe.org/3t2a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3t2a RCSB], [http://www.ebi.ac.uk/pdbsum/3t2a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3t2a ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3t2a" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 00:19, 25 December 2014

OCA — Thu, 25 Dec 2014 00:19:57 GMT

←Older revision		Revision as of 00:19, 25 December 2014
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t2a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t2a RCSB], [http://www.ebi.ac.uk/pdbsum/3t2a PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t2a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t2a RCSB], [http://www.ebi.ac.uk/pdbsum/3t2a PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/INS_PIG INS_PIG]] Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 13:32, 9 December 2014

OCA — Tue, 09 Dec 2014 13:32:35 GMT

←Older revision		Revision as of 13:32, 9 December 2014
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-	[[~~Image:~~3t2a.~~png~~\|~~left~~\|~~200px~~]]	+	==TMAO-grown cubic insulin (porcine)==
		+	<StructureSection load='3t2a' size='340' side='right' caption='[[3t2a]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3t2a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T2A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T2A FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TMO:TRIMETHYLAMINE+OXIDE'>TMO</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t25\|3t25]], [[3t26\|3t26]], [[3t27\|3t27]], [[3t28\|3t28]], [[3t29\|3t29]], [[3t2h\|3t2h]], [[3t2i\|3t2i]], [[3t2j\|3t2j]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t2a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t2a RCSB], [http://www.ebi.ac.uk/pdbsum/3t2a PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Both crystallization and cryoprotection are often bottlenecks for high-resolution X-ray structure determination of macromolecules. Methylamine osmolytes are known stabilizers of protein structure. One such osmolyte, trimethylamine N-oxide (TMAO), has seen occasional use as an additive to improve macromolecular crystal quality and has recently been shown to be an effective cryoprotective agent for low-temperature data collection. Here, TMAO and the related osmolytes sarcosine and betaine are investigated as primary precipitating agents for protein crystal growth. Crystallization experiments were undertaken with 14 proteins. Using TMAO, seven proteins crystallized in a total of 13 crystal forms, including a new tetragonal crystal form of trypsin. The crystals diffracted well, and eight of the 13 crystal forms could be effectively cryocooled as grown with TMAO as an in situ cryoprotective agent. Sarcosine and betaine produced crystals of four and two of the 14 proteins, respectively. In addition to TMAO, sarcosine and betaine were effective post-crystallization cryoprotective agents for two different crystal forms of thermolysin. Precipitation reactions of TMAO with several transition-metal ions (Fe(3+), Co(2+), Cu(2+) and Zn(2+)) did not occur with sarcosine or betaine and were inhibited for TMAO at lower pH. Structures of proteins from TMAO-grown crystals and from crystals soaked in TMAO, sarcosine or betaine were determined, showing osmolyte binding in five of the 12 crystals tested. When an osmolyte was shown to bind, it did so near the protein surface, interacting with water molecules, side chains and backbone atoms, often at crystal contacts.

-	~~{{STRUCTURE_3t2a\| PDB=3t2a \| SCENE= }}~~	+	The use of trimethylamine N-oxide as a primary precipitating agent and related methylamine osmolytes as cryoprotective agents for macromolecular crystallography.,Marshall H, Venkat M, Hti Lar Seng NS, Cahn J, Juers DH Acta Crystallogr D Biol Crystallogr. 2012 Jan;68(Pt 1):69-81. Epub 2011, Dec 9. PMID:22194335<ref>PMID:22194335</ref>

-	~~===TMAO-grown cubic insulin (porcine)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22194335}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3t2a]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Sus_scrofa Sus scrofa]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3T2A OCA].~~	+

	==See Also==		==See Also==
	*[[Molecular Playground/Insulin\|Molecular Playground/Insulin]]		*[[Molecular Playground/Insulin\|Molecular Playground/Insulin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:022194335<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]
-	[[Category: Cahn, J.]]	+	[[Category: Cahn, J]]
-	[[Category: Juers, D.]]	+	[[Category: Juers, D]]
-	[[Category: Marshall, H.]]	+	[[Category: Marshall, H]]
-	[[Category: Venkat, M.]]	+	[[Category: Venkat, M]]
	[[Category: Hormone]]		[[Category: Hormone]]

OCA at 10:36, 20 October 2012

OCA — Sat, 20 Oct 2012 10:36:07 GMT

←Older revision		Revision as of 10:36, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[3t2a]] is a 2 chain structure ~~of [[Molecular Playground/Insulin]]~~ with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T2A OCA].	+	[[3t2a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T2A OCA].

	==See Also==		==See Also==

OCA at 02:39, 26 July 2012

OCA — Thu, 26 Jul 2012 02:39:40 GMT

←Older revision		Revision as of 02:39, 26 July 2012
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	[[Image:3t2a.png\|left\|200px]]		[[Image:3t2a.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3t2a", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3t2a\| PDB=3t2a \| SCENE= }}		{{STRUCTURE_3t2a\| PDB=3t2a \| SCENE= }}

	===TMAO-grown cubic insulin (porcine)===		===TMAO-grown cubic insulin (porcine)===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_22194335}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 22194335 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_22194335}}		{{ABSTRACT_PUBMED_22194335}}

	==About this Structure==		==About this Structure==
-	[[3t2a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T2A OCA].	+	[[3t2a]] is a 2 chain structure of [[Molecular Playground/Insulin]] with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T2A OCA].
		+
		+	==See Also==
		+	*[[Molecular Playground/Insulin\|Molecular Playground/Insulin]]

	==Reference==		==Reference==

OCA at 05:32, 11 January 2012

OCA — Wed, 11 Jan 2012 05:32:18 GMT

←Older revision		Revision as of 05:32, 11 January 2012
Line 1:		Line 1:
-	[[Image:3t2a.~~jpg~~\|left\|200px]]	+	[[Image:3t2a.png\|left\|200px]]

	<!--		<!--
Line 11:		Line 11:
	===TMAO-grown cubic insulin (porcine)===		===TMAO-grown cubic insulin (porcine)===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22194335}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22194335 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22194335}}

	==About this Structure==		==About this Structure==
	[[3t2a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T2A OCA].		[[3t2a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T2A OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022194335</ref><references group="xtra"/>
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]
	[[Category: Cahn, J.]]		[[Category: Cahn, J.]]

OCA at 07:40, 28 December 2011

OCA — Wed, 28 Dec 2011 07:40:11 GMT

←Older revision		Revision as of 07:40, 28 December 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3t2a.jpg\|left\|200px]]

-	The ~~entry 3t2a~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3t2a", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3t2a\| PDB=3t2a \| SCENE= }}

-	~~Authors: Cahn, J., Venkat, M., Marshall, H., Juers, D.~~	+	===TMAO-grown cubic insulin (porcine)===

-	~~Description~~: ~~TMAO~~-~~grown cubic insulin (porcine)~~	+
		+	==About this Structure==
		+	[[3t2a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T2A OCA].
		+	[[Category: Sus scrofa]]
		+	[[Category: Cahn, J.]]
		+	[[Category: Juers, D.]]
		+	[[Category: Marshall, H.]]
		+	[[Category: Venkat, M.]]
		+	[[Category: Hormone]]

OCA at 05:53, 17 August 2011

OCA — Wed, 17 Aug 2011 05:53:02 GMT

←Older revision		Revision as of 05:53, 17 August 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3t2a is ON HOLD	+	The entry 3t2a is ON HOLD until Paper Publication

-	Authors: Cahn, J., Venkat, M., Marshall, H.	+	Authors: Cahn, J., Venkat, M., Marshall, H., Juers, D.

	Description: TMAO-grown cubic insulin (porcine)		Description: TMAO-grown cubic insulin (porcine)

OCA: Protected "3t2a" [edit=sysop:move=sysop]

OCA — Wed, 03 Aug 2011 08:24:05 GMT

Protected "3t2a" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:24, 3 August 2011