3t3n - Revision history

OCA at 13:16, 14 March 2024

OCA — Thu, 14 Mar 2024 13:16:26 GMT

←Older revision		Revision as of 13:16, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3t3n' size='340' side='right'caption='[[3t3n]], [[Resolution\|resolution]] 3.09Å' scene=''>		<StructureSection load='3t3n' size='340' side='right'caption='[[3t3n]], [[Resolution\|resolution]] 3.09Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t3n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Thet2 Thet2~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T3N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T3N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t3n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB27 Thermus thermophilus HB27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T3N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T3N FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.09Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=OMC:O2-METHYLYCYTIDINE-5-MONOPHOSPHATE'>OMC</scene>, <scene name='pdbligand=OMG:O2-METHYLGUANOSINE-5-MONOPHOSPHATE'>OMG</scene>, <scene name='pdbligand=OMU:O2-METHYLURIDINE+5-MONOPHOSPHATE'>OMU</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OMC:O2-METHYLYCYTIDINE-5-MONOPHOSPHATE'>OMC</scene>, <scene name='pdbligand=OMG:O2-METHYLGUANOSINE-5-MONOPHOSPHATE'>OMG</scene>, <scene name='pdbligand=OMU:O2-METHYLURIDINE+5-MONOPHOSPHATE'>OMU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;~~'>~~[[3bk1\|3bk1]], [[3bk2\|3bk2]], [[3t3o\|3t3o]]~~</~~div~~>~~</td></tr>~~	+
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ttc0775, TT_C0775 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=262724 THET2])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t3n OCA], [https://pdbe.org/3t3n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t3n RCSB], [https://www.ebi.ac.uk/pdbsum/3t3n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t3n ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t3n OCA], [https://pdbe.org/3t3n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t3n RCSB], [https://www.ebi.ac.uk/pdbsum/3t3n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t3n ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/RNJ_THET2 RNJ_THET2] An RNase that has endonuclease and possibly 5'-3' exonuclease activity. Probably involved in maturation of rRNA and in some organisms also mRNA maturation and/or decay.<ref>PMID:18204464</ref>
-	~~RNase J is a key member of the beta-CASP family of metallo-beta-lactamases involved in the maturation and turnover of RNAs in prokaryotes~~. ~~The B~~. ~~subtilis enzyme possesses both 5'-3' exoribonucleolytic and endonucleolytic activity, an unusual property for a ribonuclease. Here, we present the crystal structure of T. thermophilus~~ RNase ~~J bound to a 4 nucleotide RNA. The structure reveals an RNA-binding channel~~ that ~~illustrates how the enzyme functions in 5'-3' exoribonucleolytic mode and how it can function as an~~ endonuclease~~. A second, negatively charged tunnel leads from the active site,~~ and ~~is ideally located to evacuate the cleaved nucleotide in~~ 5'-3' ~~exonucleolytic mode~~. ~~We show that B. subtilis RNase J1, which shows processive behavior on long RNAs, behaves distributively for substrates less than 5 nucleotides~~ in ~~length. We propose a model involving the binding~~ of ~~the RNA to the surface of the beta-CASP domain to explain the enzyme's processive action.~~	+
-		+
-	~~Molecular basis for the recognition~~ and ~~cleavage of RNA by the bifunctional 5'-3' exo~~/~~endoribonuclease RNase J~~.~~,Dorleans A, Li de la Sierra-Gallay I, Piton J, Zig L, Gilet L, Putzer H, Condon C Structure. 2011 Sep 7;19(9):1252-61. PMID:21893286~~<ref>PMID:~~21893286~~</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3t3n" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 15:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Thet2~~]]	+	[[Category: Thermus thermophilus HB27]]
-	[[Category: Condon, C]]	+	[[Category: Condon C]]
-	[[Category: Dorleans, A]]	+	[[Category: Dorleans A]]
-	[[Category: Gilet, L]]	+	[[Category: Gilet L]]
-	[[Category: ~~Piton, J]]~~	+	[[Category: Li de la Sierra-Gallay I]]
-	~~[[Category: Putzer, H]]~~	+	[[Category: Piton J]]
-	~~[[Category:~~ Sierra-Gallay, I ~~Li de la~~]]	+	[[Category: Putzer H]]
-	[[Category: ~~Zig, L~~]]	+	[[Category: Zig L]]
-	[[Category: ~~5'-3' exoribonuclease~~]]	+
-	[[Category: ~~Endoribonuclease]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Hydrolase-rna complex]]~~	+
-	~~[[Category: Metal dependent hydrolase]]~~	+
-	~~[[Category: Metallo-beta-lactamase]]~~	+
-	~~[[Category: Protein-rna complex]]~~	+
-	~~[[Category: Rna]]~~	+
-	~~[[Category: Rnase j~~]]	+

OCA at 16:41, 6 July 2022

OCA — Wed, 06 Jul 2022 16:41:38 GMT

←Older revision		Revision as of 16:41, 6 July 2022
Line 1:		Line 1:

	==Molecular basis for the recognition and cleavage of RNA (UUCCGU) by the bifunctional 5'-3' exo/endoribonuclease RNase J==		==Molecular basis for the recognition and cleavage of RNA (UUCCGU) by the bifunctional 5'-3' exo/endoribonuclease RNase J==
-	<StructureSection load='3t3n' size='340' side='right' caption='[[3t3n]], [[Resolution\|resolution]] 3.09Å' scene=''>	+	<StructureSection load='3t3n' size='340' side='right'caption='[[3t3n]], [[Resolution\|resolution]] 3.09Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t3n]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Thet2 Thet2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T3N OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3T3N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t3n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thet2 Thet2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T3N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T3N FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OMC:O2-METHYLYCYTIDINE-5-MONOPHOSPHATE'>OMC</scene>, <scene name='pdbligand=OMG:O2-METHYLGUANOSINE-5-MONOPHOSPHATE'>OMG</scene>, <scene name='pdbligand=OMU:O2-METHYLURIDINE+5-MONOPHOSPHATE'>OMU</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OMC:O2-METHYLYCYTIDINE-5-MONOPHOSPHATE'>OMC</scene>, <scene name='pdbligand=OMG:O2-METHYLGUANOSINE-5-MONOPHOSPHATE'>OMG</scene>, <scene name='pdbligand=OMU:O2-METHYLURIDINE+5-MONOPHOSPHATE'>OMU</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bk1\|3bk1]], [[3bk2\|3bk2]], [[3t3o\|3t3o]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3bk1\|3bk1]], [[3bk2\|3bk2]], [[3t3o\|3t3o]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ttc0775, TT_C0775 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=262724 THET2])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ttc0775, TT_C0775 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=262724 THET2])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3t3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t3n OCA], [~~http~~://pdbe.org/3t3n PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3t3n RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3t3n PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3t3n ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t3n OCA], [https://pdbe.org/3t3n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t3n RCSB], [https://www.ebi.ac.uk/pdbsum/3t3n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t3n ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Thet2]]		[[Category: Thet2]]
	[[Category: Condon, C]]		[[Category: Condon, C]]

OCA at 08:58, 11 August 2016

OCA — Thu, 11 Aug 2016 08:58:55 GMT

←Older revision		Revision as of 08:58, 11 August 2016
Line 1:		Line 1:
		+
	==Molecular basis for the recognition and cleavage of RNA (UUCCGU) by the bifunctional 5'-3' exo/endoribonuclease RNase J==		==Molecular basis for the recognition and cleavage of RNA (UUCCGU) by the bifunctional 5'-3' exo/endoribonuclease RNase J==
	<StructureSection load='3t3n' size='340' side='right' caption='[[3t3n]], [[Resolution\|resolution]] 3.09Å' scene=''>		<StructureSection load='3t3n' size='340' side='right' caption='[[3t3n]], [[Resolution\|resolution]] 3.09Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t3n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Thermus_thermophilus_hb27 Thermus thermophilus hb27~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T3N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T3N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t3n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet2 Thet2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T3N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T3N FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OMC:O2-METHYLYCYTIDINE-5-MONOPHOSPHATE'>OMC</scene>, <scene name='pdbligand=OMG:O2-METHYLGUANOSINE-5-MONOPHOSPHATE'>OMG</scene>, <scene name='pdbligand=OMU:O2-METHYLURIDINE+5-MONOPHOSPHATE'>OMU</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OMC:O2-METHYLYCYTIDINE-5-MONOPHOSPHATE'>OMC</scene>, <scene name='pdbligand=OMG:O2-METHYLGUANOSINE-5-MONOPHOSPHATE'>OMG</scene>, <scene name='pdbligand=OMU:O2-METHYLURIDINE+5-MONOPHOSPHATE'>OMU</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bk1\|3bk1]], [[3bk2\|3bk2]], [[3t3o\|3t3o]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bk1\|3bk1]], [[3bk2\|3bk2]], [[3t3o\|3t3o]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ttc0775, TT_C0775 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=262724 ~~Thermus thermophilus HB27~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ttc0775, TT_C0775 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=262724 THET2])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t3n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t3n RCSB], [http://www.ebi.ac.uk/pdbsum/3t3n PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t3n OCA], [http://pdbe.org/3t3n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3t3n RCSB], [http://www.ebi.ac.uk/pdbsum/3t3n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3t3n ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3t3n" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Thermus thermophilus hb27~~]]	+	[[Category: Thet2]]
	[[Category: Condon, C]]		[[Category: Condon, C]]
	[[Category: Dorleans, A]]		[[Category: Dorleans, A]]

OCA at 09:09, 4 January 2015

OCA — Sun, 04 Jan 2015 09:09:12 GMT

←Older revision		Revision as of 09:09, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3t3n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb27 Thermus thermophilus hb27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T3N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T3N FirstGlance]. <br>		<table><tr><td colspan='2'>[[3t3n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb27 Thermus thermophilus hb27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T3N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T3N FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OMC:O2-METHYLYCYTIDINE-5-MONOPHOSPHATE'>OMC</scene>, <scene name='pdbligand=OMG:O2-METHYLGUANOSINE-5-MONOPHOSPHATE'>OMG</scene>, <scene name='pdbligand=OMU:O2-METHYLURIDINE+5-MONOPHOSPHATE'>OMU</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OMC:O2-METHYLYCYTIDINE-5-MONOPHOSPHATE'>OMC</scene>, <scene name='pdbligand=OMG:O2-METHYLGUANOSINE-5-MONOPHOSPHATE'>OMG</scene>, <scene name='pdbligand=OMU:O2-METHYLURIDINE+5-MONOPHOSPHATE'>OMU</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bk1\|3bk1]], [[3bk2\|3bk2]], [[3t3o\|3t3o]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bk1\|3bk1]], [[3bk2\|3bk2]], [[3t3o\|3t3o]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ttc0775, TT_C0775 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=262724 Thermus thermophilus HB27])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ttc0775, TT_C0775 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=262724 Thermus thermophilus HB27])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t3n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t3n RCSB], [http://www.ebi.ac.uk/pdbsum/3t3n PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t3n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t3n RCSB], [http://www.ebi.ac.uk/pdbsum/3t3n PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Thermus thermophilus hb27]]		[[Category: Thermus thermophilus hb27]]
-	[[Category: Condon, C.]]	+	[[Category: Condon, C]]
-	[[Category: Dorleans, A.]]	+	[[Category: Dorleans, A]]
-	[[Category: Gilet, L.]]	+	[[Category: Gilet, L]]
-	[[Category: Piton, J.]]	+	[[Category: Piton, J]]
-	[[Category: Putzer, H.]]	+	[[Category: Putzer, H]]
-	[[Category: Sierra-Gallay, I Li de la.]]	+	[[Category: Sierra-Gallay, I Li de la]]
-	[[Category: Zig, L.]]	+	[[Category: Zig, L]]
	[[Category: 5'-3' exoribonuclease]]		[[Category: 5'-3' exoribonuclease]]
	[[Category: Endoribonuclease]]		[[Category: Endoribonuclease]]

OCA at 06:46, 9 June 2014

OCA — Mon, 09 Jun 2014 06:46:38 GMT

←Older revision		Revision as of 06:46, 9 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3t3n.~~png~~\|~~left~~\|~~200px~~]]	+	==Molecular basis for the recognition and cleavage of RNA (UUCCGU) by the bifunctional 5'-3' exo/endoribonuclease RNase J==
		+	<StructureSection load='3t3n' size='340' side='right' caption='[[3t3n]], [[Resolution\|resolution]] 3.09Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3t3n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb27 Thermus thermophilus hb27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T3N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T3N FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OMC:O2-METHYLYCYTIDINE-5-MONOPHOSPHATE'>OMC</scene>, <scene name='pdbligand=OMG:O2-METHYLGUANOSINE-5-MONOPHOSPHATE'>OMG</scene>, <scene name='pdbligand=OMU:O2-METHYLURIDINE+5-MONOPHOSPHATE'>OMU</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3bk1\|3bk1]], [[3bk2\|3bk2]], [[3t3o\|3t3o]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ttc0775, TT_C0775 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=262724 Thermus thermophilus HB27])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t3n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t3n RCSB], [http://www.ebi.ac.uk/pdbsum/3t3n PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	RNase J is a key member of the beta-CASP family of metallo-beta-lactamases involved in the maturation and turnover of RNAs in prokaryotes. The B. subtilis enzyme possesses both 5'-3' exoribonucleolytic and endonucleolytic activity, an unusual property for a ribonuclease. Here, we present the crystal structure of T. thermophilus RNase J bound to a 4 nucleotide RNA. The structure reveals an RNA-binding channel that illustrates how the enzyme functions in 5'-3' exoribonucleolytic mode and how it can function as an endonuclease. A second, negatively charged tunnel leads from the active site, and is ideally located to evacuate the cleaved nucleotide in 5'-3' exonucleolytic mode. We show that B. subtilis RNase J1, which shows processive behavior on long RNAs, behaves distributively for substrates less than 5 nucleotides in length. We propose a model involving the binding of the RNA to the surface of the beta-CASP domain to explain the enzyme's processive action.

-	~~{{STRUCTURE_3t3n\| PDB=3t3n \| SCENE= }}~~	+	Molecular basis for the recognition and cleavage of RNA by the bifunctional 5'-3' exo/endoribonuclease RNase J.,Dorleans A, Li de la Sierra-Gallay I, Piton J, Zig L, Gilet L, Putzer H, Condon C Structure. 2011 Sep 7;19(9):1252-61. PMID:21893286<ref>PMID:21893286</ref>

-	~~===Molecular basis for the recognition and cleavage~~ of ~~RNA (UUCCGU) by~~ the ~~bifunctional 5'-3' exo/endoribonuclease RNase J===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_21893286}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3t3n]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Thermus_thermophilus_hb27 Thermus thermophilus hb27]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3T3N OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:021893286<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Thermus thermophilus hb27]]		[[Category: Thermus thermophilus hb27]]
	[[Category: Condon, C.]]		[[Category: Condon, C.]]

OCA at 05:34, 29 August 2012

OCA — Wed, 29 Aug 2012 05:34:36 GMT

←Older revision		Revision as of 05:34, 29 August 2012
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	[[Image:3t3n.png\|left\|200px]]		[[Image:3t3n.png\|left\|200px]]

-	<!--
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-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3t3n\| PDB=3t3n \| SCENE= }}		{{STRUCTURE_3t3n\| PDB=3t3n \| SCENE= }}

	===Molecular basis for the recognition and cleavage of RNA (UUCCGU) by the bifunctional 5'-3' exo/endoribonuclease RNase J===		===Molecular basis for the recognition and cleavage of RNA (UUCCGU) by the bifunctional 5'-3' exo/endoribonuclease RNase J===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_21893286}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 21893286 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_21893286}}		{{ABSTRACT_PUBMED_21893286}}

OCA at 10:21, 30 October 2011

OCA — Sun, 30 Oct 2011 10:21:39 GMT

←Older revision		Revision as of 10:21, 30 October 2011
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-	~~'''Unreleased structure'''~~	+	[[Image:3t3n.png\|left\|200px]]

-	The ~~entry 3t3n~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3t3n", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3t3n\| PDB=3t3n \| SCENE= }}

-	~~Authors: Dorleans, A., Li de la Sierra~~-~~Gallay, I., Piton,~~ J~~., Zig, L., Gilet, L., Putzer, H., Condon, C.~~	+	===Molecular basis for the recognition and cleavage of RNA (UUCCGU) by the bifunctional 5'-3' exo/endoribonuclease RNase J===

-	~~Description: Molecular basis for~~ the ~~recognition and cleavage of RNA~~ (~~UUCCGU~~) by the ~~bifunctional~~ 5'-3' ~~exo/endoribonuclease RNase J~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_21893286}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 21893286 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_21893286}}
		+
		+	==About this Structure==
		+	[[3t3n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb27 Thermus thermophilus hb27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T3N OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:021893286</ref><references group="xtra"/>
		+	[[Category: Thermus thermophilus hb27]]
		+	[[Category: Condon, C.]]
		+	[[Category: Dorleans, A.]]
		+	[[Category: Gilet, L.]]
		+	[[Category: Piton, J.]]
		+	[[Category: Putzer, H.]]
		+	[[Category: Sierra-Gallay, I Li de la.]]
		+	[[Category: Zig, L.]]
		+	[[Category: 5'-3' exoribonuclease]]
		+	[[Category: Endoribonuclease]]
		+	[[Category: Hydrolase]]
		+	[[Category: Hydrolase-rna complex]]
		+	[[Category: Metal dependent hydrolase]]
		+	[[Category: Metallo-beta-lactamase]]
		+	[[Category: Protein-rna complex]]
		+	[[Category: Rna]]
		+	[[Category: Rnase j]]

OCA at 06:20, 31 August 2011

OCA — Wed, 31 Aug 2011 06:20:48 GMT

←Older revision		Revision as of 06:20, 31 August 2011
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3t3n is ON HOLD	+	The entry 3t3n is ON HOLD until Paper Publication

-	Authors: Dorleans, A., de la Sierra-Gallay, I.L., Piton, J., Zig, L., Gilet, L., Putzer, H., Condon, C.	+	Authors: Dorleans, A., Li de la Sierra-Gallay, I., Piton, J., Zig, L., Gilet, L., Putzer, H., Condon, C.

	Description: Molecular basis for the recognition and cleavage of RNA (UUCCGU) by the bifunctional 5'-3' exo/endoribonuclease RNase J		Description: Molecular basis for the recognition and cleavage of RNA (UUCCGU) by the bifunctional 5'-3' exo/endoribonuclease RNase J

OCA: Protected "3t3n" [edit=sysop:move=sysop]

OCA — Wed, 03 Aug 2011 08:25:41 GMT

Protected "3t3n" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:25, 3 August 2011

OCA: New page: '''Unreleased structure''' The entry 3t3n is ON HOLD Authors: Dorleans, A., de la Sierra-Gallay, I.L., Piton, J., Zig, L., Gilet, L., Putzer, H., Condon, C. Description: Molecular basi...

OCA — Wed, 03 Aug 2011 08:25:40 GMT

New page: '''Unreleased structure''' The entry 3t3n is ON HOLD Authors: Dorleans, A., de la Sierra-Gallay, I.L., Piton, J., Zig, L., Gilet, L., Putzer, H., Condon, C. Description: Molecular basi...

New page

'''Unreleased structure'''

The entry 3t3n is ON HOLD

Authors: Dorleans, A., de la Sierra-Gallay, I.L., Piton, J., Zig, L., Gilet, L., Putzer, H., Condon, C.

Description: Molecular basis for the recognition and cleavage of RNA (UUCCGU) by the bifunctional 5'-3' exo/endoribonuclease RNase J