3t47 - Revision history

OCA at 13:17, 14 March 2024

OCA — Thu, 14 Mar 2024 13:17:06 GMT

←Older revision		Revision as of 13:17, 14 March 2024
Line 3:		Line 3:
	<StructureSection load='3t47' size='340' side='right'caption='[[3t47]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='3t47' size='340' side='right'caption='[[3t47]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t47]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Staam Staam~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T47 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T47 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t47]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_Mu50 Staphylococcus aureus subsp. aureus Mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T47 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T47 FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[3t46\|3t46]], [[3t48\|3t48]], [[3t49\|3t49]], [[3t4a\|3t4a]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.301Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">SAV0229 ([https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=158878 STAAM])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t47 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t47 OCA], [https://pdbe.org/3t47 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t47 RCSB], [https://www.ebi.ac.uk/pdbsum/3t47 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t47 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t47 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t47 OCA], [https://pdbe.org/3t47 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t47 RCSB], [https://www.ebi.ac.uk/pdbsum/3t47 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t47 ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/A0A0H3JPM4_STAAM A0A0H3JPM4_STAAM] Involved in countering the first line of host defense mechanisms. Efficiently inhibits opsonization, phagocytosis and killing of S.aureus by human neutrophils. Acts by binding and stabilizing human C3 convertases (C4b2a and C3bBb), leading to their inactivation. The convertases are no longer able to cleave complement C3, therefore preventing further C3b deposition on the bacterial surface and phagocytosis of the bacterium. Also prevents C5a-induced neutrophil responses.[ARBA:ARBA00025334]
-	To survive in immune-competent hosts, the pathogen Staphylococcus aureus expresses and secretes a sophisticated array of proteins that inhibit the complement system. Among these are the staphylococcal complement inhibitors (SCIN), which are composed of three active proteins (SCIN-A, -B, and -C) and one purportedly inactive member (SCIN-D or ORF-D). Because previous work has focused almost exclusively on SCIN-A, we sought to provide initial structure/~~function information on additional SCIN proteins~~. ~~To this end we determined crystal structures of an active, N-terminal truncation mutant of SCIN-B (denoted SCIN-B(18-85)) both free and bound to the C3c fragment of complement component C3 at 1~~.~~5 and 3.4 A resolution, respectively. Comparison of the C3c~~/~~SCIN-B(18-85) structure with that of C3c~~/~~SCIN-A revealed that both proteins target the same functional hotspot on the C3b/C3c surface yet harbor diversity~~ in ~~both~~ the ~~type~~ of ~~residues and interactions formed at their C3b/C3c interfaces~~. ~~Most importantly~~, ~~these structures allowed identification~~ of ~~Arg~~(~~44)~~ and ~~Tyr(51~~) ~~as residues key for SCIN-B binding to C3b and subsequent inhibition of the AP C3 convertase. In addition~~, ~~we also solved several crystal structures of SCIN-D~~ to 1.~~3 A limiting resolution. This revealed an unexpected structural deviation in the N-terminal alpha helix relative~~ to ~~SCIN-A and SCIN-B. Comparative analysis of both electrostatic potentials and surface complementarity suggest a physical explanation for the inability of SCIN-D to bind~~ C3b/C3c. Together, these studies provide a more thorough understanding of immune evasion by S. aureus and enhance potential use of SCIN proteins as templates for design of complement targeted therapeutics.	+
-		+
-	~~Diversity in~~ the ~~C3b Convertase Contact Residues~~ and ~~Tertiary Structures~~ of the ~~Staphylococcal Complement Inhibitor (SCIN) Protein Family~~.~~,Garcia BL, Summers BJ, Lin Z, Ramyar KX, Ricklin D, Kamath DV, Fu ZQ, Lambris JD, Geisbrecht BV J Biol Chem. 2012 Jan 2;287(1):628~~-40. ~~Epub 2011 Nov 15. PMID~~:~~22086928<ref>PMID:22086928</ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3t47" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Staam~~]]	+	[[Category: Staphylococcus aureus subsp. aureus Mu50]]
-	[[Category: Garcia~~, B L~~]]	+	[[Category: Garcia BL]]
-	[[Category: Geisbrecht~~, B V~~]]	+	[[Category: Geisbrecht BV]]
-	[[Category: Summers~~, B J]]~~	+	[[Category: Summers BJ]]
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Secreted]]~~	+
-	~~[[Category: Virulence~~]]	+

OCA at 16:42, 6 July 2022

OCA — Wed, 06 Jul 2022 16:42:18 GMT

←Older revision		Revision as of 16:42, 6 July 2022
Line 1:		Line 1:

	==Crystal Structure of truncated form of Staphylococcal Complement Inhibitor D (SCIN-D) at 1.3 Angstrom==		==Crystal Structure of truncated form of Staphylococcal Complement Inhibitor D (SCIN-D) at 1.3 Angstrom==
-	<StructureSection load='3t47' size='340' side='right' caption='[[3t47]], [[Resolution\|resolution]] 1.30Å' scene=''>	+	<StructureSection load='3t47' size='340' side='right'caption='[[3t47]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t47]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Staam Staam]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T47 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3T47 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t47]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staam Staam]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T47 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T47 FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t46\|3t46]], [[3t48\|3t48]], [[3t49\|3t49]], [[3t4a\|3t4a]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3t46\|3t46]], [[3t48\|3t48]], [[3t49\|3t49]], [[3t4a\|3t4a]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAV0229 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158878 STAAM])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAV0229 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158878 STAAM])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3t47 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t47 OCA], [~~http~~://pdbe.org/3t47 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3t47 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3t47 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3t47 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t47 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t47 OCA], [https://pdbe.org/3t47 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t47 RCSB], [https://www.ebi.ac.uk/pdbsum/3t47 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t47 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Staam]]		[[Category: Staam]]
	[[Category: Garcia, B L]]		[[Category: Garcia, B L]]

OCA at 19:39, 11 August 2016

OCA — Thu, 11 Aug 2016 19:39:06 GMT

←Older revision		Revision as of 19:39, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal Structure of truncated form of Staphylococcal Complement Inhibitor D (SCIN-D) at 1.3 Angstrom==		==Crystal Structure of truncated form of Staphylococcal Complement Inhibitor D (SCIN-D) at 1.3 Angstrom==
	<StructureSection load='3t47' size='340' side='right' caption='[[3t47]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='3t47' size='340' side='right' caption='[[3t47]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t47]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Staphylococcus_aureus_subsp._aureus Staphylococcus aureus subsp. aureus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T47 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T47 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t47]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staam Staam]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T47 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T47 FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t46\|3t46]], [[3t48\|3t48]], [[3t49\|3t49]], [[3t4a\|3t4a]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t46\|3t46]], [[3t48\|3t48]], [[3t49\|3t49]], [[3t4a\|3t4a]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAV0229 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~46170 Staphylococcus aureus subsp. aureus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAV0229 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158878 STAAM])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t47 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t47 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t47 RCSB], [http://www.ebi.ac.uk/pdbsum/3t47 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t47 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t47 OCA], [http://pdbe.org/3t47 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3t47 RCSB], [http://www.ebi.ac.uk/pdbsum/3t47 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3t47 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3t47" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Staphylococcus aureus subsp. aureus~~]]	+	[[Category: Staam]]
	[[Category: Garcia, B L]]		[[Category: Garcia, B L]]
	[[Category: Geisbrecht, B V]]		[[Category: Geisbrecht, B V]]

OCA at 09:15, 4 January 2015

OCA — Sun, 04 Jan 2015 09:15:38 GMT

←Older revision		Revision as of 09:15, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3t47]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus Staphylococcus aureus subsp. aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T47 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T47 FirstGlance]. <br>		<table><tr><td colspan='2'>[[3t47]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus Staphylococcus aureus subsp. aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T47 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T47 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t46\|3t46]], [[3t48\|3t48]], [[3t49\|3t49]], [[3t4a\|3t4a]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t46\|3t46]], [[3t48\|3t48]], [[3t49\|3t49]], [[3t4a\|3t4a]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAV0229 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=46170 Staphylococcus aureus subsp. aureus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAV0229 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=46170 Staphylococcus aureus subsp. aureus])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t47 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t47 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t47 RCSB], [http://www.ebi.ac.uk/pdbsum/3t47 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t47 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t47 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t47 RCSB], [http://www.ebi.ac.uk/pdbsum/3t47 PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 13:		Line 13:
	Diversity in the C3b Convertase Contact Residues and Tertiary Structures of the Staphylococcal Complement Inhibitor (SCIN) Protein Family.,Garcia BL, Summers BJ, Lin Z, Ramyar KX, Ricklin D, Kamath DV, Fu ZQ, Lambris JD, Geisbrecht BV J Biol Chem. 2012 Jan 2;287(1):628-40. Epub 2011 Nov 15. PMID:22086928<ref>PMID:22086928</ref>		Diversity in the C3b Convertase Contact Residues and Tertiary Structures of the Staphylococcal Complement Inhibitor (SCIN) Protein Family.,Garcia BL, Summers BJ, Lin Z, Ramyar KX, Ricklin D, Kamath DV, Fu ZQ, Lambris JD, Geisbrecht BV J Biol Chem. 2012 Jan 2;287(1):628-40. Epub 2011 Nov 15. PMID:22086928<ref>PMID:22086928</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 20:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Staphylococcus aureus subsp. aureus]]		[[Category: Staphylococcus aureus subsp. aureus]]
-	[[Category: Garcia, B L.]]	+	[[Category: Garcia, B L]]
-	[[Category: Geisbrecht, B V.]]	+	[[Category: Geisbrecht, B V]]
-	[[Category: Summers, B J.]]	+	[[Category: Summers, B J]]
	[[Category: Immune system]]		[[Category: Immune system]]
	[[Category: Secreted]]		[[Category: Secreted]]
	[[Category: Virulence]]		[[Category: Virulence]]

OCA at 05:48, 5 June 2014

OCA — Thu, 05 Jun 2014 05:48:25 GMT

←Older revision		Revision as of 05:48, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3t47.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal Structure of truncated form of Staphylococcal Complement Inhibitor D (SCIN-D) at 1.3 Angstrom==
		+	<StructureSection load='3t47' size='340' side='right' caption='[[3t47]], [[Resolution\|resolution]] 1.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3t47]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus Staphylococcus aureus subsp. aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T47 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T47 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t46\|3t46]], [[3t48\|3t48]], [[3t49\|3t49]], [[3t4a\|3t4a]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAV0229 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=46170 Staphylococcus aureus subsp. aureus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t47 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t47 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t47 RCSB], [http://www.ebi.ac.uk/pdbsum/3t47 PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	To survive in immune-competent hosts, the pathogen Staphylococcus aureus expresses and secretes a sophisticated array of proteins that inhibit the complement system. Among these are the staphylococcal complement inhibitors (SCIN), which are composed of three active proteins (SCIN-A, -B, and -C) and one purportedly inactive member (SCIN-D or ORF-D). Because previous work has focused almost exclusively on SCIN-A, we sought to provide initial structure/function information on additional SCIN proteins. To this end we determined crystal structures of an active, N-terminal truncation mutant of SCIN-B (denoted SCIN-B(18-85)) both free and bound to the C3c fragment of complement component C3 at 1.5 and 3.4 A resolution, respectively. Comparison of the C3c/SCIN-B(18-85) structure with that of C3c/SCIN-A revealed that both proteins target the same functional hotspot on the C3b/C3c surface yet harbor diversity in both the type of residues and interactions formed at their C3b/C3c interfaces. Most importantly, these structures allowed identification of Arg(44) and Tyr(51) as residues key for SCIN-B binding to C3b and subsequent inhibition of the AP C3 convertase. In addition, we also solved several crystal structures of SCIN-D to 1.3 A limiting resolution. This revealed an unexpected structural deviation in the N-terminal alpha helix relative to SCIN-A and SCIN-B. Comparative analysis of both electrostatic potentials and surface complementarity suggest a physical explanation for the inability of SCIN-D to bind C3b/C3c. Together, these studies provide a more thorough understanding of immune evasion by S. aureus and enhance potential use of SCIN proteins as templates for design of complement targeted therapeutics.

-	~~<!--~~	+	Diversity in the C3b Convertase Contact Residues and Tertiary Structures of the Staphylococcal Complement Inhibitor (SCIN) Protein Family.,Garcia BL, Summers BJ, Lin Z, Ramyar KX, Ricklin D, Kamath DV, Fu ZQ, Lambris JD, Geisbrecht BV J Biol Chem. 2012 Jan 2;287(1):628-40. Epub 2011 Nov 15. PMID:22086928<ref>PMID:22086928</ref>
-	~~The line below this paragraph, containing "STRUCTURE_3t47", creates~~ the ~~"Structure Box" on the page.~~	+
-	~~You may change~~ the ~~PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter~~ (~~which sets the initial scene displayed when the page is loaded~~),	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3t47\| PDB=3t47 \| SCENE= }}~~	+

-	~~===Crystal Structure~~ of ~~truncated form of Staphylococcal Complement Inhibitor D (SCIN-D) at 1.3 Angstrom===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22086928}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22086928 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_22086928}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3t47]] is a 2 chain structure with sequence from [http:~~//en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus Staphylococcus aureus subsp. aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T47 OCA].	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022086928<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Staphylococcus aureus subsp. aureus]]		[[Category: Staphylococcus aureus subsp. aureus]]
	[[Category: Garcia, B L.]]		[[Category: Garcia, B L.]]

OCA at 09:57, 18 January 2012

OCA — Wed, 18 Jan 2012 09:57:24 GMT

←Older revision		Revision as of 09:57, 18 January 2012
Line 1:		Line 1:
-	[[Image:3t47.~~jpg~~\|left\|200px]]	+	[[Image:3t47.png\|left\|200px]]

	<!--		<!--
Line 11:		Line 11:
	===Crystal Structure of truncated form of Staphylococcal Complement Inhibitor D (SCIN-D) at 1.3 Angstrom===		===Crystal Structure of truncated form of Staphylococcal Complement Inhibitor D (SCIN-D) at 1.3 Angstrom===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22086928}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22086928 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22086928}}

	==About this Structure==		==About this Structure==
	[[3t47]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus Staphylococcus aureus subsp. aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T47 OCA].		[[3t47]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus Staphylococcus aureus subsp. aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T47 OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022086928</ref><references group="xtra"/>
	[[Category: Staphylococcus aureus subsp. aureus]]		[[Category: Staphylococcus aureus subsp. aureus]]
	[[Category: Garcia, B L.]]		[[Category: Garcia, B L.]]

OCA at 19:16, 23 November 2011

OCA — Wed, 23 Nov 2011 19:16:08 GMT

←Older revision		Revision as of 19:16, 23 November 2011
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3t47.jpg\|left\|200px]]

-	The ~~entry 3t47~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3t47", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3t47\| PDB=3t47 \| SCENE= }}

-	~~Authors: Garcia, B.L., Geisbrecht, B.V., Summers, B.J~~.	+	===Crystal Structure of truncated form of Staphylococcal Complement Inhibitor D (SCIN-D) at 1.3 Angstrom===

-	~~Description: Crystal~~ Structure ~~of truncated form of Staphylococcal Complement Inhibitor D (SCIN~~-~~D) at 1~~.~~3 Angstrom~~	+
		+	==About this Structure==
		+	[[3t47]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus Staphylococcus aureus subsp. aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T47 OCA].
		+	[[Category: Staphylococcus aureus subsp. aureus]]
		+	[[Category: Garcia, B L.]]
		+	[[Category: Geisbrecht, B V.]]
		+	[[Category: Summers, B J.]]
		+	[[Category: Immune system]]
		+	[[Category: Secreted]]
		+	[[Category: Virulence]]

OCA at 07:08, 24 August 2011

OCA — Wed, 24 Aug 2011 07:08:33 GMT

←Older revision		Revision as of 07:08, 24 August 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3t47 is ON HOLD	+	The entry 3t47 is ON HOLD until Paper Publication

	Authors: Garcia, B.L., Geisbrecht, B.V., Summers, B.J.		Authors: Garcia, B.L., Geisbrecht, B.V., Summers, B.J.

-	Description: ~~Evidence for diversity in both the C3b contact residues and tertiary structures~~ of ~~the staphylococcal complement inhibitor~~ (SCIN) ~~protein family~~	+	Description: Crystal Structure of truncated form of Staphylococcal Complement Inhibitor D (SCIN-D) at 1.3 Angstrom

OCA at 05:33, 10 August 2011

OCA — Wed, 10 Aug 2011 05:33:59 GMT

←Older revision		Revision as of 05:33, 10 August 2011
Line 5:		Line 5:
	Authors: Garcia, B.L., Geisbrecht, B.V., Summers, B.J.		Authors: Garcia, B.L., Geisbrecht, B.V., Summers, B.J.

-	Description: ~~EVIDENCE FOR DIVERSITY IN BOTH THE C3B CONTACT RESIDUES AND TERTIARY STRUCTURES OF THE STAPHYLOCOCCAL COMPLEMENT INHIBITOR~~ (SCIN) ~~PROTEIN FAMILY~~	+	Description: Evidence for diversity in both the C3b contact residues and tertiary structures of the staphylococcal complement inhibitor (SCIN) protein family

OCA: Protected "3t47" [edit=sysop:move=sysop]

OCA — Wed, 03 Aug 2011 08:26:21 GMT

Protected "3t47" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:26, 3 August 2011