3t5m - Revision history

OCA at 10:03, 1 March 2024

OCA — Fri, 01 Mar 2024 10:03:00 GMT

←Older revision		Revision as of 10:03, 1 March 2024
Line 3:		Line 3:
	<StructureSection load='3t5m' size='340' side='right'caption='[[3t5m]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='3t5m' size='340' side='right'caption='[[3t5m]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t5m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bacillus_anthracis_(strain_ames)~~ Bacillus anthracis ~~(strain ames)~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T5M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T5M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t5m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis_str._Ames Bacillus anthracis str. Ames]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T5M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T5M FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>~~, ~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.749Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~BAS1809, BA_1949, GBAA_1949 ([https~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=198094 Bacillus anthracis (strain Ames)])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t5m OCA], [https://pdbe.org/3t5m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t5m RCSB], [https://www.ebi.ac.uk/pdbsum/3t5m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t5m ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t5m OCA], [https://pdbe.org/3t5m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t5m RCSB], [https://www.ebi.ac.uk/pdbsum/3t5m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t5m ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/A0A6L8PEJ7_BACAN A0A6L8PEJ7_BACAN]
-	Microcin C (McC) is heptapeptide adenylate antibiotic produced by Escherichia coli strains carrying the mccABCDEF gene cluster encoding enzymes, in addition to the heptapeptide structural gene mccA, necessary for McC biosynthesis and self-immunity of the producing cell. The heptapeptide facilitates McC transport into susceptible cells, where it is processed releasing a non-hydrolyzable aminoacyl adenylate that inhibits an essential aminoacyl-tRNA synthetase. The self-immunity gene mccF encodes a specialized serine peptidase that cleaves an amide bond connecting the peptidyl or aminoacyl moieties of, respectively, intact and processed McC with the nucleotidyl moiety. Most mccF orthologs from organisms other than E. coli are not linked to the McC biosynthesis gene cluster. Here, we show that a protein product of one such gene, MccF from Bacillus anthracis (BaMccF), is able to cleave intact and processed McC, and we present a series of structures of this protein. Structural analysis of apo-BaMccF and its adenosine monophosphate complex reveals specific features of MccF-like peptidases that allow them to interact with substrates containing nucleotidyl moieties. Sequence analyses and phylogenetic reconstructions suggest that several distinct subfamilies form the MccF clade of the large S66 family of bacterial serine peptidases. We show that various representatives of the MccF clade can specifically detoxify non-hydrolyzable aminoacyl adenylates differing in their aminoacyl moieties. We hypothesize that bacterial mccF genes serve as a source of bacterial antibiotic resistance.	+
-		+
-	Structural and Functional Characterization of Microcin C Resistance Peptidase MccF from Bacillus anthracis.,Nocek B, Tikhonov A, Babnigg G, Gu M, Zhou M, Makarova KS, Vondenhoff G, Aerschot AV, Kwon K, Anderson WF, Severinov K, Joachimiak A J Mol Biol. 2012 Apr 16. PMID:~~22516613<ref>PMID:22516613<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 3t5m" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus anthracis str. Ames]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Anderson~~, W F]]~~	+	[[Category: Anderson WF]]
-	~~[[Category: Structural genomic~~]]	+	[[Category: Gu M]]
-	[[Category: Gu, M]]	+	[[Category: Joachimiak A]]
-	[[Category: Joachimiak, A]]	+	[[Category: Nocek B]]
-	[[Category: Nocek, B]]	+	[[Category: Zhou M]]
-	[[Category: Zhou, M]]	+
-	~~[[Category: Amp]]~~	+
-	~~[[Category: Csgid]]~~	+
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Mccf~~]]	+

OCA at 16:44, 6 July 2022

OCA — Wed, 06 Jul 2022 16:44:05 GMT

←Older revision		Revision as of 16:44, 6 July 2022
Line 1:		Line 1:

	==Crystal structure of the S112A mutant of mycrocine immunity protein (MccF) with AMP==		==Crystal structure of the S112A mutant of mycrocine immunity protein (MccF) with AMP==
-	<StructureSection load='3t5m' size='340' side='right' caption='[[3t5m]], [[Resolution\|resolution]] 1.75Å' scene=''>	+	<StructureSection load='3t5m' size='340' side='right'caption='[[3t5m]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t5m]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bacillus_anthracis_(strain_ames) Bacillus anthracis (strain ames)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T5M OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3T5M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t5m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis_(strain_ames) Bacillus anthracis (strain ames)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T5M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T5M FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BAS1809, BA_1949, GBAA_1949 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198094 Bacillus anthracis (strain Ames)])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BAS1809, BA_1949, GBAA_1949 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198094 Bacillus anthracis (strain Ames)])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3t5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t5m OCA], [~~http~~://pdbe.org/3t5m PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3t5m RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3t5m PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3t5m ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t5m OCA], [https://pdbe.org/3t5m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t5m RCSB], [https://www.ebi.ac.uk/pdbsum/3t5m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t5m ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Anderson, W F]]		[[Category: Anderson, W F]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]

OCA at 09:42, 11 August 2016

OCA — Thu, 11 Aug 2016 09:42:49 GMT

←Older revision		Revision as of 09:42, 11 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of the S112A mutant of mycrocine immunity protein (MccF) with AMP==		==Crystal structure of the S112A mutant of mycrocine immunity protein (MccF) with AMP==
	<StructureSection load='3t5m' size='340' side='right' caption='[[3t5m]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='3t5m' size='340' side='right' caption='[[3t5m]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t5m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_anthracis~~ Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T5M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T5M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t5m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis_(strain_ames) Bacillus anthracis (strain ames)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T5M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T5M FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BAS1809, BA_1949, GBAA_1949 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~1392~~ Bacillus anthracis])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BAS1809, BA_1949, GBAA_1949 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198094 Bacillus anthracis (strain Ames)])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t5m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t5m RCSB], [http://www.ebi.ac.uk/pdbsum/3t5m PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t5m OCA], [http://pdbe.org/3t5m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3t5m RCSB], [http://www.ebi.ac.uk/pdbsum/3t5m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3t5m ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 15:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3t5m" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus anthracis]]
	[[Category: Anderson, W F]]		[[Category: Anderson, W F]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]

OCA at 10:05, 4 January 2015

OCA — Sun, 04 Jan 2015 10:05:31 GMT

←Older revision		Revision as of 10:05, 4 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3t5m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T5M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T5M FirstGlance]. <br>		<table><tr><td colspan='2'>[[3t5m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T5M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T5M FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BAS1809, BA_1949, GBAA_1949 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 Bacillus anthracis])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BAS1809, BA_1949, GBAA_1949 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 Bacillus anthracis])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t5m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t5m RCSB], [http://www.ebi.ac.uk/pdbsum/3t5m PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t5m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t5m RCSB], [http://www.ebi.ac.uk/pdbsum/3t5m PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 13:		Line 13:
	Structural and Functional Characterization of Microcin C Resistance Peptidase MccF from Bacillus anthracis.,Nocek B, Tikhonov A, Babnigg G, Gu M, Zhou M, Makarova KS, Vondenhoff G, Aerschot AV, Kwon K, Anderson WF, Severinov K, Joachimiak A J Mol Biol. 2012 Apr 16. PMID:22516613<ref>PMID:22516613</ref>		Structural and Functional Characterization of Microcin C Resistance Peptidase MccF from Bacillus anthracis.,Nocek B, Tikhonov A, Babnigg G, Gu M, Zhou M, Makarova KS, Vondenhoff G, Aerschot AV, Kwon K, Anderson WF, Severinov K, Joachimiak A J Mol Biol. 2012 Apr 16. PMID:22516613<ref>PMID:22516613</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 20:		Line 20:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus anthracis]]		[[Category: Bacillus anthracis]]
-	[[Category: Anderson, W F.]]	+	[[Category: Anderson, W F]]
-	[[Category: ~~CSGID, Center for~~ Structural ~~Genomics of Infectious Diseases.~~]]	+	[[Category: Structural genomic]]
-	[[Category: Gu, M.]]	+	[[Category: Gu, M]]
-	[[Category: Joachimiak, A.]]	+	[[Category: Joachimiak, A]]
-	[[Category: Nocek, B.]]	+	[[Category: Nocek, B]]
-	[[Category: Zhou, M.]]	+	[[Category: Zhou, M]]
	[[Category: Amp]]		[[Category: Amp]]
-	[[Category: Center for structural genomics of infectious disease]]
	[[Category: Csgid]]		[[Category: Csgid]]
	[[Category: Immune system]]		[[Category: Immune system]]
	[[Category: Mccf]]		[[Category: Mccf]]
-	[[Category: Structural genomic]]

OCA at 06:40, 5 June 2014

OCA — Thu, 05 Jun 2014 06:40:23 GMT

←Older revision		Revision as of 06:40, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3t5m.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of the S112A mutant of mycrocine immunity protein (MccF) with AMP==
		+	<StructureSection load='3t5m' size='340' side='right' caption='[[3t5m]], [[Resolution\|resolution]] 1.75Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3t5m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T5M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T5M FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BAS1809, BA_1949, GBAA_1949 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 Bacillus anthracis])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t5m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t5m RCSB], [http://www.ebi.ac.uk/pdbsum/3t5m PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Microcin C (McC) is heptapeptide adenylate antibiotic produced by Escherichia coli strains carrying the mccABCDEF gene cluster encoding enzymes, in addition to the heptapeptide structural gene mccA, necessary for McC biosynthesis and self-immunity of the producing cell. The heptapeptide facilitates McC transport into susceptible cells, where it is processed releasing a non-hydrolyzable aminoacyl adenylate that inhibits an essential aminoacyl-tRNA synthetase. The self-immunity gene mccF encodes a specialized serine peptidase that cleaves an amide bond connecting the peptidyl or aminoacyl moieties of, respectively, intact and processed McC with the nucleotidyl moiety. Most mccF orthologs from organisms other than E. coli are not linked to the McC biosynthesis gene cluster. Here, we show that a protein product of one such gene, MccF from Bacillus anthracis (BaMccF), is able to cleave intact and processed McC, and we present a series of structures of this protein. Structural analysis of apo-BaMccF and its adenosine monophosphate complex reveals specific features of MccF-like peptidases that allow them to interact with substrates containing nucleotidyl moieties. Sequence analyses and phylogenetic reconstructions suggest that several distinct subfamilies form the MccF clade of the large S66 family of bacterial serine peptidases. We show that various representatives of the MccF clade can specifically detoxify non-hydrolyzable aminoacyl adenylates differing in their aminoacyl moieties. We hypothesize that bacterial mccF genes serve as a source of bacterial antibiotic resistance.

-	~~{{STRUCTURE_3t5m\| PDB=3t5m \| SCENE= }}~~	+	Structural and Functional Characterization of Microcin C Resistance Peptidase MccF from Bacillus anthracis.,Nocek B, Tikhonov A, Babnigg G, Gu M, Zhou M, Makarova KS, Vondenhoff G, Aerschot AV, Kwon K, Anderson WF, Severinov K, Joachimiak A J Mol Biol. 2012 Apr 16. PMID:22516613<ref>PMID:22516613</ref>

-	~~===Crystal structure~~ of the ~~S112A mutant of mycrocine immunity protein (MccF) with AMP===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22516613}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[3t5m]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bacillus_anthracis Bacillus anthracis]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=3T5M OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022516613<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Bacillus anthracis]]		[[Category: Bacillus anthracis]]
	[[Category: Anderson, W F.]]		[[Category: Anderson, W F.]]

OCA at 06:22, 25 July 2012

OCA — Wed, 25 Jul 2012 06:22:51 GMT

←Older revision		Revision as of 06:22, 25 July 2012
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	[[Image:3t5m.png\|left\|200px]]		[[Image:3t5m.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_3t5m", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_3t5m\| PDB=3t5m \| SCENE= }}		{{STRUCTURE_3t5m\| PDB=3t5m \| SCENE= }}

	===Crystal structure of the S112A mutant of mycrocine immunity protein (MccF) with AMP===		===Crystal structure of the S112A mutant of mycrocine immunity protein (MccF) with AMP===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_22516613}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 22516613 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_22516613}}		{{ABSTRACT_PUBMED_22516613}}

OCA at 18:25, 2 May 2012

OCA — Wed, 02 May 2012 18:25:21 GMT

←Older revision		Revision as of 18:25, 2 May 2012
Line 1:		Line 1:
-	[[Image:3t5m.~~jpg~~\|left\|200px]]	+	[[Image:3t5m.png\|left\|200px]]

	<!--		<!--
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	===Crystal structure of the S112A mutant of mycrocine immunity protein (MccF) with AMP===		===Crystal structure of the S112A mutant of mycrocine immunity protein (MccF) with AMP===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22516613}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22516613 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22516613}}

	==About this Structure==		==About this Structure==
	[[3t5m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T5M OCA].		[[3t5m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T5M OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022516613</ref><references group="xtra"/>
	[[Category: Bacillus anthracis]]		[[Category: Bacillus anthracis]]
	[[Category: Anderson, W F.]]		[[Category: Anderson, W F.]]

OCA at 06:21, 7 September 2011

OCA — Wed, 07 Sep 2011 06:21:26 GMT

←Older revision		Revision as of 06:21, 7 September 2011
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-	~~'''Unreleased structure'''~~	+	[[Image:3t5m.jpg\|left\|200px]]

-	The ~~entry 3t5m~~ is ~~ON HOLD~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3t5m", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3t5m\| PDB=3t5m \| SCENE= }}

-	~~Authors: Nocek, B., Zhou, M., Gu, M., Anderson, W.F., Joachimiak, A., Center for Structural Genomics~~ of ~~Infectious Diseases~~ (~~CSGID~~)	+	===Crystal structure of the S112A mutant of mycrocine immunity protein (MccF) with AMP===

-	~~Description: Crystal~~ structure of ~~the S112A mutant~~ of ~~mycrocine immunity protein (MccF) with AMP~~	+
		+	==About this Structure==
		+	[[3t5m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T5M OCA].
		+	[[Category: Bacillus anthracis]]
		+	[[Category: Anderson, W F.]]
		+	[[Category: CSGID, Center for Structural Genomics of Infectious Diseases.]]
		+	[[Category: Gu, M.]]
		+	[[Category: Joachimiak, A.]]
		+	[[Category: Nocek, B.]]
		+	[[Category: Zhou, M.]]
		+	[[Category: Amp]]
		+	[[Category: Center for structural genomics of infectious disease]]
		+	[[Category: Csgid]]
		+	[[Category: Immune system]]
		+	[[Category: Mccf]]
		+	[[Category: Structural genomic]]

OCA at 05:34, 10 August 2011

OCA — Wed, 10 Aug 2011 05:34:41 GMT

←Older revision		Revision as of 05:34, 10 August 2011
Line 3:		Line 3:
	The entry 3t5m is ON HOLD		The entry 3t5m is ON HOLD

-	Authors: Nocek, B., Zhou, M., Gu, M., Anderson, W. F., Joachimiak, A.~~, CSGID~~, Center for Structural Genomics of Infectious Diseases (CSGID)	+	Authors: Nocek, B., Zhou, M., Gu, M., Anderson, W.F., Joachimiak, A., Center for Structural Genomics of Infectious Diseases (CSGID)

-	Description: Crystal structure of the S112A mutant of mycrocine immunity protein (MccF) with AMP.	+	Description: Crystal structure of the S112A mutant of mycrocine immunity protein (MccF) with AMP

OCA: Protected "3t5m" [edit=sysop:move=sysop]

OCA — Wed, 03 Aug 2011 08:28:01 GMT

Protected "3t5m" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:28, 3 August 2011