3t5q - Revision history

OCA at 10:03, 1 March 2024

OCA — Fri, 01 Mar 2024 10:03:04 GMT

←Older revision		Revision as of 10:03, 1 March 2024
Line 3:		Line 3:
	<StructureSection load='3t5q' size='340' side='right'caption='[[3t5q]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='3t5q' size='340' side='right'caption='[[3t5q]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t5q]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/~~Mopeia_lassa_reassortant_29~~ Mopeia ~~lassa~~ reassortant 29]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T5Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T5Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t5q]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Mopeia_Lassa_virus_reassortant_29 Mopeia Lassa virus reassortant 29]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T5Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T5Q FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[3t5n\|3t5n]]~~<~~/div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">NP ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300180 Mopeia Lassa reassortant 29])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t5q OCA], [https://pdbe.org/3t5q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t5q RCSB], [https://www.ebi.ac.uk/pdbsum/3t5q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t5q ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t5q OCA], [https://pdbe.org/3t5q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t5q RCSB], [https://www.ebi.ac.uk/pdbsum/3t5q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t5q ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/Q5S585_9VIRU Q5S585_9VIRU]] Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses (By similarity).[PIRNR:PIRNR004029]	+	[https://www.uniprot.org/uniprot/Q5S585_9VIRU Q5S585_9VIRU] Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses (By similarity).[PIRNR:PIRNR004029]
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Arenaviruses cause disease in industrialized and developing nations alike. Among them, the hemorrhagic fever virus Lassa is responsible for ~300,000-500,000 infections/y in Western Africa. The arenavirus nucleoprotein (NP) forms the protein scaffold of the genomic ribonucleoprotein complexes and is critical for transcription and replication of the viral genome. Here, we present crystal structures of the RNA-binding domain of Lassa virus NP in complex with ssRNA. This structure shows, in contrast to the predicted model, that RNA binds in a deep, basic crevice located entirely within the N-terminal domain. Furthermore, the NP-ssRNA structures presented here, combined with hydrogen-deuterium exchange/MS and functional studies, suggest a gating mechanism by which NP opens to accept RNA. Directed mutagenesis and functional studies provide a unique look into how the arenavirus NPs bind to and protect the viral genome and also suggest the likely assembly by which viral ribonucleoprotein complexes are organized.	+
-		+
-	Crystal structure of the Lassa virus nucleoprotein-RNA complex reveals a gating mechanism for RNA binding.,Hastie KM, Liu T, Li S, King LB, Ngo N, Zandonatti MA, Woods VL Jr, de la Torre JC, Saphire EO Proc Natl Acad Sci U S A. 2011 Nov 29;108(48):19365-70. Epub 2011 Nov 14. PMID:22084115<ref>PMID:22084115</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3t5q" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Nucleoprotein 3D structures\|Nucleoprotein 3D structures]]		*[[Nucleoprotein 3D structures\|Nucleoprotein 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Mopeia ~~lassa~~ reassortant 29]]	+	[[Category: Mopeia Lassa virus reassortant 29]]
-	[[Category: Hastie~~, K M~~]]	+	[[Category: Hastie KM]]
-	[[Category: King~~, L B~~]]	+	[[Category: King LB]]
-	[[Category: Liu, T]]	+	[[Category: Liu T]]
-	[[Category: Ngo, N]]	+	[[Category: Ngo N]]
-	[[Category: Saphire~~, E O]]~~	+	[[Category: Saphire EO]]
-	~~[[Category: Torre, J C.de la~~]]	+	[[Category: Woods VL]]
-	[[Category: Woods~~, V L~~]]	+	[[Category: Zandonatti MA]]
-	[[Category: Zandonatti~~, M A~~]]	+	[[Category: De la Torre JC]]
-	[[Category: ~~Single stranded rna]]~~	+
-	~~[[Category: Ssrna]]~~	+
-	~~[[Category: Viral protein-rna complex~~]]	+

OCA at 16:44, 6 July 2022

OCA — Wed, 06 Jul 2022 16:44:15 GMT

←Older revision		Revision as of 16:44, 6 July 2022
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	==3A structure of Lassa virus nucleoprotein in complex with ssRNA==		==3A structure of Lassa virus nucleoprotein in complex with ssRNA==
-	<StructureSection load='3t5q' size='340' side='right' caption='[[3t5q]], [[Resolution\|resolution]] 3.00Å' scene=''>	+	<StructureSection load='3t5q' size='340' side='right'caption='[[3t5q]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t5q]] is a 12 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [~~http~~://en.wikipedia.org/wiki/Mopeia_lassa_reassortant_29 Mopeia lassa reassortant 29]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T5Q OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3T5Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t5q]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Mopeia_lassa_reassortant_29 Mopeia lassa reassortant 29]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T5Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T5Q FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t5n\|3t5n]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3t5n\|3t5n]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NP ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300180 Mopeia Lassa reassortant 29])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NP ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300180 Mopeia Lassa reassortant 29])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3t5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t5q OCA], [~~http~~://pdbe.org/3t5q PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3t5q RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3t5q PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3t5q ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t5q OCA], [https://pdbe.org/3t5q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t5q RCSB], [https://www.ebi.ac.uk/pdbsum/3t5q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t5q ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/Q5S585_9VIRU Q5S585_9VIRU]] Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses (By similarity).[PIRNR:PIRNR004029]	+	[[https://www.uniprot.org/uniprot/Q5S585_9VIRU Q5S585_9VIRU]] Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses (By similarity).[PIRNR:PIRNR004029]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:

	==See Also==		==See Also==
-	*[[Nucleoprotein\|Nucleoprotein]]	+	*[[Nucleoprotein 3D structures\|Nucleoprotein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 28:		Line 28:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
		+	[[Category: Large Structures]]
	[[Category: Mopeia lassa reassortant 29]]		[[Category: Mopeia lassa reassortant 29]]
	[[Category: Hastie, K M]]		[[Category: Hastie, K M]]

OCA at 18:43, 5 August 2016

OCA — Fri, 05 Aug 2016 18:43:40 GMT

←Older revision		Revision as of 18:43, 5 August 2016
Line 1:		Line 1:
		+
	==3A structure of Lassa virus nucleoprotein in complex with ssRNA==		==3A structure of Lassa virus nucleoprotein in complex with ssRNA==
	<StructureSection load='3t5q' size='340' side='right' caption='[[3t5q]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='3t5q' size='340' side='right' caption='[[3t5q]], [[Resolution\|resolution]] 3.00Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t5n\|3t5n]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t5n\|3t5n]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300180 Mopeia Lassa reassortant 29])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300180 Mopeia Lassa reassortant 29])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t5q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t5q RCSB], [http://www.ebi.ac.uk/pdbsum/3t5q PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t5q OCA], [http://pdbe.org/3t5q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3t5q RCSB], [http://www.ebi.ac.uk/pdbsum/3t5q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3t5q ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3t5q" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 04:55, 25 December 2014

OCA — Thu, 25 Dec 2014 04:55:08 GMT

←Older revision		Revision as of 04:55, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3t5q]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Mopeia_lassa_reassortant_29 Mopeia lassa reassortant 29]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T5Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T5Q FirstGlance]. <br>		<table><tr><td colspan='2'>[[3t5q]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Mopeia_lassa_reassortant_29 Mopeia lassa reassortant 29]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T5Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T5Q FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t5n\|3t5n]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t5n\|3t5n]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300180 Mopeia Lassa reassortant 29])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300180 Mopeia Lassa reassortant 29])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t5q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t5q RCSB], [http://www.ebi.ac.uk/pdbsum/3t5q PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t5q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t5q RCSB], [http://www.ebi.ac.uk/pdbsum/3t5q PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q5S585_9VIRU Q5S585_9VIRU]] Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses (By similarity).[PIRNR:PIRNR004029]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 14:		Line 16:
	Crystal structure of the Lassa virus nucleoprotein-RNA complex reveals a gating mechanism for RNA binding.,Hastie KM, Liu T, Li S, King LB, Ngo N, Zandonatti MA, Woods VL Jr, de la Torre JC, Saphire EO Proc Natl Acad Sci U S A. 2011 Nov 29;108(48):19365-70. Epub 2011 Nov 14. PMID:22084115<ref>PMID:22084115</ref>		Crystal structure of the Lassa virus nucleoprotein-RNA complex reveals a gating mechanism for RNA binding.,Hastie KM, Liu T, Li S, King LB, Ngo N, Zandonatti MA, Woods VL Jr, de la Torre JC, Saphire EO Proc Natl Acad Sci U S A. 2011 Nov 29;108(48):19365-70. Epub 2011 Nov 14. PMID:22084115<ref>PMID:22084115</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>

Line 25:		Line 27:
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Mopeia lassa reassortant 29]]		[[Category: Mopeia lassa reassortant 29]]
-	[[Category: Hastie, K M.]]	+	[[Category: Hastie, K M]]
-	[[Category: King, L B.]]	+	[[Category: King, L B]]
-	[[Category: Liu, T.]]	+	[[Category: Liu, T]]
-	[[Category: Ngo, N.]]	+	[[Category: Ngo, N]]
-	[[Category: Saphire, E O.]]	+	[[Category: Saphire, E O]]
-	[[Category: Torre, J C.de la.]]	+	[[Category: Torre, J C.de la]]
-	[[Category: Woods, V L.]]	+	[[Category: Woods, V L]]
-	[[Category: Zandonatti, M A.]]	+	[[Category: Zandonatti, M A]]
	[[Category: Single stranded rna]]		[[Category: Single stranded rna]]
	[[Category: Ssrna]]		[[Category: Ssrna]]
	[[Category: Viral protein-rna complex]]		[[Category: Viral protein-rna complex]]

OCA at 06:09, 5 June 2014

OCA — Thu, 05 Jun 2014 06:09:54 GMT

←Older revision		Revision as of 06:09, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3t5q.~~png~~\|~~left~~\|~~200px~~]]	+	==3A structure of Lassa virus nucleoprotein in complex with ssRNA==
		+	<StructureSection load='3t5q' size='340' side='right' caption='[[3t5q]], [[Resolution\|resolution]] 3.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3t5q]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Mopeia_lassa_reassortant_29 Mopeia lassa reassortant 29]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T5Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T5Q FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t5n\|3t5n]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300180 Mopeia Lassa reassortant 29])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t5q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t5q RCSB], [http://www.ebi.ac.uk/pdbsum/3t5q PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Arenaviruses cause disease in industrialized and developing nations alike. Among them, the hemorrhagic fever virus Lassa is responsible for ~300,000-500,000 infections/y in Western Africa. The arenavirus nucleoprotein (NP) forms the protein scaffold of the genomic ribonucleoprotein complexes and is critical for transcription and replication of the viral genome. Here, we present crystal structures of the RNA-binding domain of Lassa virus NP in complex with ssRNA. This structure shows, in contrast to the predicted model, that RNA binds in a deep, basic crevice located entirely within the N-terminal domain. Furthermore, the NP-ssRNA structures presented here, combined with hydrogen-deuterium exchange/MS and functional studies, suggest a gating mechanism by which NP opens to accept RNA. Directed mutagenesis and functional studies provide a unique look into how the arenavirus NPs bind to and protect the viral genome and also suggest the likely assembly by which viral ribonucleoprotein complexes are organized.

-	<!--	+	Crystal structure of the Lassa virus nucleoprotein-RNA complex reveals a gating mechanism for RNA binding.,Hastie KM, Liu T, Li S, King LB, Ngo N, Zandonatti MA, Woods VL Jr, de la Torre JC, Saphire EO Proc Natl Acad Sci U S A. 2011 Nov 29;108(48):19365-70. Epub 2011 Nov 14. PMID:22084115<ref>PMID:22084115</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3t5q"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display~~.	+
-	-->	+
-	~~{{STRUCTURE_3t5q\| PDB=3t5q \| SCENE= }}~~	+

-	~~===3A structure~~ of ~~Lassa virus nucleoprotein in complex with ssRNA===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-		+	==See Also==
-	~~<!--~~	+	*[[Nucleoprotein\|Nucleoprotein]]
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22084115}}, adds the Publication Abstract to the page~~	+	== References ==
-	~~(as it appears on PubMed at http://www.pubmed.gov), where 22084115 is the PubMed ID number.~~	+	<references/>
-	~~-->~~	+	__TOC__
-	~~{{ABSTRACT_PUBMED_22084115}}~~	+	</StructureSection>
-		+
-	==~~About this Structure~~==	+
-	[[~~3t5q~~]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Mopeia_lassa_reassortant_29 Mopeia lassa reassortant 29]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T5Q OCA].	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022084115<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Mopeia lassa reassortant 29]]		[[Category: Mopeia lassa reassortant 29]]

OCA at 06:20, 11 January 2012

OCA — Wed, 11 Jan 2012 06:20:16 GMT

←Older revision		Revision as of 06:20, 11 January 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3t5q.png\|left\|200px]]

-	The ~~entry 3t5q~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3t5q", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3t5q\| PDB=3t5q \| SCENE= }}

-	~~Authors: Hastie, K.M., Liu, T., King, L.B., Ngo, N., Zandonatti, M.A., Woods, V.L., de la Torre, J.C., Saphire, E.O.~~	+	===3A structure of Lassa virus nucleoprotein in complex with ssRNA===

-	~~Description~~: 3A structure ~~of Lassa virus nucleoprotein in complex~~ with ~~ssRNA~~	+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22084115}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22084115 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22084115}}
		+
		+	==About this Structure==
		+	[[3t5q]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Mopeia_lassa_reassortant_29 Mopeia lassa reassortant 29]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T5Q OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022084115</ref><references group="xtra"/>
		+	[[Category: Escherichia coli]]
		+	[[Category: Mopeia lassa reassortant 29]]
		+	[[Category: Hastie, K M.]]
		+	[[Category: King, L B.]]
		+	[[Category: Liu, T.]]
		+	[[Category: Ngo, N.]]
		+	[[Category: Saphire, E O.]]
		+	[[Category: Torre, J C.de la.]]
		+	[[Category: Woods, V L.]]
		+	[[Category: Zandonatti, M A.]]
		+	[[Category: Single stranded rna]]
		+	[[Category: Ssrna]]
		+	[[Category: Viral protein-rna complex]]

OCA at 08:38, 30 October 2011

OCA — Sun, 30 Oct 2011 08:38:28 GMT

←Older revision		Revision as of 08:38, 30 October 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3t5q is ON HOLD	+	The entry 3t5q is ON HOLD until Paper Publication

-	Authors: Hastie, K.M. , Liu, T., King, L.B., Ngo, N. , Zandonatti, M.A., Woods, V.L., de la Torre, J.C., Saphire, E.O.	+	Authors: Hastie, K.M., Liu, T., King, L.B., Ngo, N., Zandonatti, M.A., Woods, V.L., de la Torre, J.C., Saphire, E.O.

	Description: 3A structure of Lassa virus nucleoprotein in complex with ssRNA		Description: 3A structure of Lassa virus nucleoprotein in complex with ssRNA

OCA: Protected "3t5q" [edit=sysop:move=sysop]

OCA — Wed, 03 Aug 2011 08:28:09 GMT

Protected "3t5q" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:28, 3 August 2011

OCA: New page: '''Unreleased structure''' The entry 3t5q is ON HOLD Authors: Hastie, K.M. , Liu, T., King, L.B., Ngo, N. , Zandonatti, M.A., Woods, V.L., de la Torre, J.C., Saphire, E.O. Description:...

OCA — Wed, 03 Aug 2011 08:28:08 GMT

New page: '''Unreleased structure''' The entry 3t5q is ON HOLD Authors: Hastie, K.M. , Liu, T., King, L.B., Ngo, N. , Zandonatti, M.A., Woods, V.L., de la Torre, J.C., Saphire, E.O. Description:...

New page

'''Unreleased structure'''

The entry 3t5q is ON HOLD

Authors: Hastie, K.M. , Liu, T., King, L.B., Ngo, N. , Zandonatti, M.A., Woods, V.L., de la Torre, J.C., Saphire, E.O.

Description: 3A structure of Lassa virus nucleoprotein in complex with ssRNA