3t7j - Revision history

OCA at 08:37, 20 March 2024

OCA — Wed, 20 Mar 2024 08:37:22 GMT

←Older revision		Revision as of 08:37, 20 March 2024
Line 3:		Line 3:
	<StructureSection load='3t7j' size='340' side='right'caption='[[3t7j]], [[Resolution\|resolution]] 2.04Å' scene=''>		<StructureSection load='3t7j' size='340' side='right'caption='[[3t7j]], [[Resolution\|resolution]] 2.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t7j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Baker's_yeast Baker's yeast~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T7J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T7J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t7j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T7J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T7J FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[3t7i\|3t7i]], [[3t7k\|3t7k]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.042Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">RTT107 ([https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=559292 Baker's yeast])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t7j OCA], [https://pdbe.org/3t7j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t7j RCSB], [https://www.ebi.ac.uk/pdbsum/3t7j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t7j ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t7j OCA], [https://pdbe.org/3t7j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t7j RCSB], [https://www.ebi.ac.uk/pdbsum/3t7j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t7j ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/RT107_YEAST RT107_YEAST]] Required for resumption of chromosome replication after DNA damage, specifically in S phase. Is recruited to chromatin in the presence of RTT109 and RTT101 in response to stalled replication forks and acts as a scaffold during DNA repair.<ref>PMID:14988729</ref> <ref>PMID:17978089</ref>	+	[https://www.uniprot.org/uniprot/RT107_YEAST RT107_YEAST] Required for resumption of chromosome replication after DNA damage, specifically in S phase. Is recruited to chromatin in the presence of RTT109 and RTT101 in response to stalled replication forks and acts as a scaffold during DNA repair.<ref>PMID:14988729</ref> <ref>PMID:17978089</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Rtt107 (regulator of Ty1 transposition 107; Esc4) is a DNA repair protein from Saccharomyces cerevisiae that can restore stalled replication forks following DNA damage. There are six BRCT (BRCA1 C-terminal) domains in Rtt107 that act as binding sites for other recruited proteins during DNA repair. Several Rtt107 binding partners have been identified, including Slx4, Rtt101, Rad55, and the Smc5/6 (structural maintenance of chromosome) protein complex. Rtt107 can reportedly be recruited to chromatin in the presence of Rtt101 and Rtt109 upon DNA damage, but the chromatin-binding site of Rtt107 has not been identified. Here, we report our investigation of the interaction between phosphorylated histone H2A (gammaH2A) and the C-terminal tandem BRCT repeats (BRCT(5)-BRCT(6)) of Rtt107. The crystal structures of BRCT(5)-BRCT(6) alone and in a complex with gammaH2A reveal the molecular basis of the Rtt107-gammaH2A interaction. We used in vitro mutagenesis and a fluorescence polarization assay to confirm the location of the Rtt107 motif that is crucial for this interaction. In addition, these assays indicated that this interaction requires the phosphorylation of H2A. An in vivo phenotypic analysis in yeast demonstrated the critical role of BRCT(5)-BRCT(6) and its interaction with gammaH2A during the DNA damage response. Our results shed new light on the molecular mechanism by which Rtt107 is recruited to chromatin in response to stalled DNA replication forks.	+
-		+
-	Structure of C-terminal tandem BRCT repeats of Rtt107 protein reveals critical role in interaction with phosphorylated histone H2A during DNA damage repair.,Li X, Liu K, Li F, Wang J, Huang H, Wu J, Shi Y J Biol Chem. 2012 Mar 16;287(12):9137-46. Epub 2012 Jan 19. PMID:22262834<ref>PMID:22262834</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 3t7j" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Baker's yeast]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Li, F]]	+	[[Category: Saccharomyces cerevisiae S288C]]
-	[[Category: Li, X]]	+	[[Category: Li F]]
-	[[Category: Shi, Y]]	+	[[Category: Li X]]
-	[[Category: Wu, J]]	+	[[Category: Shi Y]]
-	~~[[Category: Brct]]~~	+	[[Category: Wu J]]
-	~~[[Category: Dna repair]]~~	+
-	~~[[Category: Phospho-peptide]]~~	+
-	~~[[Category: Protein binding~~]]	+

OCA at 16:46, 6 July 2022

OCA — Wed, 06 Jul 2022 16:46:21 GMT

←Older revision		Revision as of 16:46, 6 July 2022
Line 1:		Line 1:

	==Crystal structure of Rtt107p (residues 820-1070)==		==Crystal structure of Rtt107p (residues 820-1070)==
-	<StructureSection load='3t7j' size='340' side='right' caption='[[3t7j]], [[Resolution\|resolution]] 2.04Å' scene=''>	+	<StructureSection load='3t7j' size='340' side='right'caption='[[3t7j]], [[Resolution\|resolution]] 2.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t7j]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T7J OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3T7J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t7j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T7J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3T7J FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t7i\|3t7i]], [[3t7k\|3t7k]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3t7i\|3t7i]], [[3t7k\|3t7k]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RTT107 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RTT107 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=3t7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t7j OCA], [~~http~~://pdbe.org/3t7j PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=3t7j RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/3t7j PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=3t7j ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3t7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t7j OCA], [https://pdbe.org/3t7j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3t7j RCSB], [https://www.ebi.ac.uk/pdbsum/3t7j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3t7j ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/RT107_YEAST RT107_YEAST]] Required for resumption of chromosome replication after DNA damage, specifically in S phase. Is recruited to chromatin in the presence of RTT109 and RTT101 in response to stalled replication forks and acts as a scaffold during DNA repair.<ref>PMID:14988729</ref> <ref>PMID:17978089</ref>	+	[[https://www.uniprot.org/uniprot/RT107_YEAST RT107_YEAST]] Required for resumption of chromosome replication after DNA damage, specifically in S phase. Is recruited to chromatin in the presence of RTT109 and RTT101 in response to stalled replication forks and acts as a scaffold during DNA repair.<ref>PMID:14988729</ref> <ref>PMID:17978089</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Baker's yeast]]		[[Category: Baker's yeast]]
		+	[[Category: Large Structures]]
	[[Category: Li, F]]		[[Category: Li, F]]
	[[Category: Li, X]]		[[Category: Li, X]]

OCA at 15:07, 4 August 2016

OCA — Thu, 04 Aug 2016 15:07:52 GMT

←Older revision		Revision as of 15:07, 4 August 2016
Line 1:		Line 1:
		+
	==Crystal structure of Rtt107p (residues 820-1070)==		==Crystal structure of Rtt107p (residues 820-1070)==
	<StructureSection load='3t7j' size='340' side='right' caption='[[3t7j]], [[Resolution\|resolution]] 2.04Å' scene=''>		<StructureSection load='3t7j' size='340' side='right' caption='[[3t7j]], [[Resolution\|resolution]] 2.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[3t7j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae Saccharomyces cerevisiae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T7J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T7J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[3t7j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T7J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T7J FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t7i\|3t7i]], [[3t7k\|3t7k]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t7i\|3t7i]], [[3t7k\|3t7k]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RTT107 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~4932 Saccharomyces cerevisiae~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RTT107 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t7j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t7j RCSB], [http://www.ebi.ac.uk/pdbsum/3t7j PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t7j OCA], [http://pdbe.org/3t7j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3t7j RCSB], [http://www.ebi.ac.uk/pdbsum/3t7j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3t7j ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 18:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 3t7j" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Saccharomyces cerevisiae~~]]	+	[[Category: Baker's yeast]]
	[[Category: Li, F]]		[[Category: Li, F]]
	[[Category: Li, X]]		[[Category: Li, X]]

OCA at 10:24, 25 December 2014

OCA — Thu, 25 Dec 2014 10:24:02 GMT

←Older revision		Revision as of 10:24, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[3t7j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T7J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T7J FirstGlance]. <br>		<table><tr><td colspan='2'>[[3t7j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T7J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T7J FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t7i\|3t7i]], [[3t7k\|3t7k]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t7i\|3t7i]], [[3t7k\|3t7k]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RTT107 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RTT107 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t7j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t7j RCSB], [http://www.ebi.ac.uk/pdbsum/3t7j PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t7j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t7j RCSB], [http://www.ebi.ac.uk/pdbsum/3t7j PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RT107_YEAST RT107_YEAST]] Required for resumption of chromosome replication after DNA damage, specifically in S phase. Is recruited to chromatin in the presence of RTT109 and RTT101 in response to stalled replication forks and acts as a scaffold during DNA repair.<ref>PMID:14988729</ref> <ref>PMID:17978089</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 13:		Line 15:
	Structure of C-terminal tandem BRCT repeats of Rtt107 protein reveals critical role in interaction with phosphorylated histone H2A during DNA damage repair.,Li X, Liu K, Li F, Wang J, Huang H, Wu J, Shi Y J Biol Chem. 2012 Mar 16;287(12):9137-46. Epub 2012 Jan 19. PMID:22262834<ref>PMID:22262834</ref>		Structure of C-terminal tandem BRCT repeats of Rtt107 protein reveals critical role in interaction with phosphorylated histone H2A during DNA damage repair.,Li X, Liu K, Li F, Wang J, Huang H, Wu J, Shi Y J Biol Chem. 2012 Mar 16;287(12):9137-46. Epub 2012 Jan 19. PMID:22262834<ref>PMID:22262834</ref>

-	From ~~MEDLINEÂ®~~/~~PubMedÂ®~~, a database of the U.S. National Library of Medicine.<br>	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
	== References ==		== References ==
Line 20:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
-	[[Category: Li, F.]]	+	[[Category: Li, F]]
-	[[Category: Li, X.]]	+	[[Category: Li, X]]
-	[[Category: Shi, Y.]]	+	[[Category: Shi, Y]]
-	[[Category: Wu, J.]]	+	[[Category: Wu, J]]
	[[Category: Brct]]		[[Category: Brct]]
	[[Category: Dna repair]]		[[Category: Dna repair]]
	[[Category: Phospho-peptide]]		[[Category: Phospho-peptide]]
	[[Category: Protein binding]]		[[Category: Protein binding]]

OCA at 06:17, 5 June 2014

OCA — Thu, 05 Jun 2014 06:17:54 GMT

←Older revision		Revision as of 06:17, 5 June 2014
Line 1:		Line 1:
-	[[~~Image:~~3t7j.~~png~~\|~~left~~\|~~200px~~]]	+	==Crystal structure of Rtt107p (residues 820-1070)==
		+	<StructureSection load='3t7j' size='340' side='right' caption='[[3t7j]], [[Resolution\|resolution]] 2.04Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[3t7j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T7J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3T7J FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3t7i\|3t7i]], [[3t7k\|3t7k]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RTT107 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3t7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3t7j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3t7j RCSB], [http://www.ebi.ac.uk/pdbsum/3t7j PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Rtt107 (regulator of Ty1 transposition 107; Esc4) is a DNA repair protein from Saccharomyces cerevisiae that can restore stalled replication forks following DNA damage. There are six BRCT (BRCA1 C-terminal) domains in Rtt107 that act as binding sites for other recruited proteins during DNA repair. Several Rtt107 binding partners have been identified, including Slx4, Rtt101, Rad55, and the Smc5/6 (structural maintenance of chromosome) protein complex. Rtt107 can reportedly be recruited to chromatin in the presence of Rtt101 and Rtt109 upon DNA damage, but the chromatin-binding site of Rtt107 has not been identified. Here, we report our investigation of the interaction between phosphorylated histone H2A (gammaH2A) and the C-terminal tandem BRCT repeats (BRCT(5)-BRCT(6)) of Rtt107. The crystal structures of BRCT(5)-BRCT(6) alone and in a complex with gammaH2A reveal the molecular basis of the Rtt107-gammaH2A interaction. We used in vitro mutagenesis and a fluorescence polarization assay to confirm the location of the Rtt107 motif that is crucial for this interaction. In addition, these assays indicated that this interaction requires the phosphorylation of H2A. An in vivo phenotypic analysis in yeast demonstrated the critical role of BRCT(5)-BRCT(6) and its interaction with gammaH2A during the DNA damage response. Our results shed new light on the molecular mechanism by which Rtt107 is recruited to chromatin in response to stalled DNA replication forks.

-	<!--	+	Structure of C-terminal tandem BRCT repeats of Rtt107 protein reveals critical role in interaction with phosphorylated histone H2A during DNA damage repair.,Li X, Liu K, Li F, Wang J, Huang H, Wu J, Shi Y J Biol Chem. 2012 Mar 16;287(12):9137-46. Epub 2012 Jan 19. PMID:22262834<ref>PMID:22262834</ref>
-	~~The line below this paragraph~~, ~~containing "STRUCTURE_3t7j"~~, ~~creates the "Structure Box" on the page~~.	+
-	~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)	+
-	~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~	+
-	~~or leave the SCENE parameter empty for the default display.~~	+
-	-->	+
-	~~{{STRUCTURE_3t7j\| PDB=3t7j \| SCENE= }}~~	+

-	~~===Crystal structure~~ of ~~Rtt107p (residues 820-1070)===~~	+	From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-		+	== References ==
-	~~<!--~~	+	<references/>
-	~~The line below this paragraph, {{ABSTRACT_PUBMED_22262834}}, adds~~ the ~~Publication Abstract to the page~~	+	__TOC__
-	~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22262834 is the PubMed ID number~~.	+	</StructureSection>
-	-->	+
-	~~{{ABSTRACT_PUBMED_22262834}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[3t7j]] is a 2 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T7J OCA].~~	+
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:022262834<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Li, F.]]		[[Category: Li, F.]]

OCA at 08:03, 25 April 2012

OCA — Wed, 25 Apr 2012 08:03:09 GMT

←Older revision		Revision as of 08:03, 25 April 2012
Line 1:		Line 1:
-	[[Image:3t7j.~~jpg~~\|left\|200px]]	+	[[Image:3t7j.png\|left\|200px]]

	<!--		<!--
Line 11:		Line 11:
	===Crystal structure of Rtt107p (residues 820-1070)===		===Crystal structure of Rtt107p (residues 820-1070)===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_22262834}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 22262834 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_22262834}}

	==About this Structure==		==About this Structure==
	[[3t7j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T7J OCA].		[[3t7j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T7J OCA].
		+
		+	==Reference==
		+	<ref group="xtra">PMID:022262834</ref><references group="xtra"/>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Li, F.]]		[[Category: Li, F.]]

OCA at 06:52, 15 February 2012

OCA — Wed, 15 Feb 2012 06:52:32 GMT

←Older revision		Revision as of 06:52, 15 February 2012
Line 1:		Line 1:
-	~~'''Unreleased structure'''~~	+	[[Image:3t7j.jpg\|left\|200px]]

-	The ~~entry 3t7j~~ is ~~ON HOLD~~ ~~until Paper Publication~~	+	<!--
		+	The line below this paragraph, containing "STRUCTURE_3t7j", creates the "Structure Box" on the page.
		+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
		+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
		+	or leave the SCENE parameter empty for the default display.
		+	-->
		+	{{STRUCTURE_3t7j\| PDB=3t7j \| SCENE= }}

-	~~Authors: Li, X., Li, F., Wu, J., Shi, Y.~~	+	===Crystal structure of Rtt107p (residues 820-1070)===

-	~~Description:~~ Structure of a ~~yeast protein~~	+
		+	==About this Structure==
		+	[[3t7j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3T7J OCA].
		+	[[Category: Saccharomyces cerevisiae]]
		+	[[Category: Li, F.]]
		+	[[Category: Li, X.]]
		+	[[Category: Shi, Y.]]
		+	[[Category: Wu, J.]]
		+	[[Category: Brct]]
		+	[[Category: Dna repair]]
		+	[[Category: Phospho-peptide]]
		+	[[Category: Protein binding]]

OCA at 05:45, 7 September 2011

OCA — Wed, 07 Sep 2011 05:45:21 GMT

←Older revision		Revision as of 05:45, 7 September 2011
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 3t7j is ON HOLD	+	The entry 3t7j is ON HOLD until Paper Publication

	Authors: Li, X., Li, F., Wu, J., Shi, Y.		Authors: Li, X., Li, F., Wu, J., Shi, Y.

	Description: Structure of a yeast protein		Description: Structure of a yeast protein

OCA at 05:54, 17 August 2011

OCA — Wed, 17 Aug 2011 05:54:32 GMT

←Older revision		Revision as of 05:54, 17 August 2011
Line 3:		Line 3:
	The entry 3t7j is ON HOLD		The entry 3t7j is ON HOLD

-	Authors: ~~Xinxin~~ Li, ~~Fudong~~ Li, ~~Jihui~~ Wu, ~~Yunyu~~ Shi	+	Authors: Li, X., Li, F., Wu, J., Shi, Y.

	Description: Structure of a yeast protein		Description: Structure of a yeast protein

OCA: Protected "3t7j" [edit=sysop:move=sysop]

OCA — Wed, 10 Aug 2011 05:36:05 GMT

Protected "3t7j" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:36, 10 August 2011